对大冶铁矿黄石国家矿山公园景观应用的设想

国家矿山公园的建立主要是针对资源枯竭型矿山的可持续发展提供心得思路。论文从矿山景观资源的类型分析入手,探讨大冶铁矿的公园景观资源,针对大冶铁矿景观资源特点,提出应用途径,为大冶铁矿黄山国家矿山公园的建设提供开发和设计依据。     

Separation and relationship of ten marine scuticociliates (Protozoa, Ciliophora) using RAPD fingerprinting method

On the basis of the random amplified polymorphic DNA-fingerprinting (RAPD) method, 10 morphospecies of scuticociliates from 7 genera, including 15 clones of 13 strains, Uronema marinum, Uronema cf marinum, Parauronema virginianum, P. longum, Metanophrys similis, M. sinensis, Paralembus digitiformis, Mesanophrys carcini, Paranophrys magna and Cohnilernbus verrninus were analysed using 8 oligonucleotide primers. The genetic similarity among the clones of the same strain measured by a band-sharing index is 0.97～0.98,while 0.40-4).52 among strains. This value measured is 0.39～0.46 among congeners of the same genus, whereas 0.16～0.47 between different genera. A distance tree was constructed based on 8-primer analysis, in which the scuticociliates investigated were separated into two clusters: one consists of 2 genera, Uronema and Parauronema, and the other was composed of five, Metanophrys, Mestmophrys,Paranophrys, Paralembus and Cohnilembus. It is demonstrated also that the morphospecies Parauronema virginianurn may be a species-complex, i. e., it contains different genospecies.     

日本(虫寻)和底栖短桨蟹线粒体DNA 12SrRNA基因序列的初步研究

以相应引物对日本和底栖短桨蟹的线粒体 DNA1 2 S r RNA基因片段进行了 PCR扩增和序列测定 ,分析比较了 2种间序列差异。结果表明 :日本 1 2 S r RNA基因片段长度为 41 4 bp,底栖短桨蟹为 41 5bp,2种的 A,T,G,C含量分别为 1 51 bp(36.47% ) ,1 53bp(36.96% ) ,43bp(1 0 .39% ) ,67bp(1 6.1 8% )和 1 50 bp(36.1 4 % ) ,1 55bp(37.35% ) ,44bp(1 0 .60 % ) ,66bp(1 5.90 % )。 2种间共出现了 3个碱基的缺失 /插入和 38bp的序列差异 ,其碱基转换与碱基颠换比约为 2 .1 7。     

江西省龟峰丹霞地貌景观类型与成因分析

龟峰丹霞地貌区位于信江盆地南缘,地处扬子板块与华夏板块的结合带.景区内丹霞地貌景观类型齐全,成景层位为上白垩统龟峰群的河口组和塘边组.河口组以紫红、砖红色砾岩、砂岩为主,塘边组以紫红色砂岩、粉砂岩、泥岩为主.本文介绍了区内丹霞地貌类型,并结合对该区地质特征研究,提出岩性差异、地层产状、构造发育和水流影响为龟峰丹霞地貌形成的主要因素.     

转“全鱼”溶菌酶基因大菱鲆的研究

用大西洋条鳕(Macrozoarces americanus)抗冻蛋白启动子opAFP promoter和牙鲆(Paralichthys olivaceus)c型溶菌酶基因构建“全鱼”溶菌酶基因元件opAFP—ly，首次应用电脉冲精子介导法将该基因片段导入到大菱鲆(Scophthalmus maximus)受精卵内，获得原代转基因大菱鲆。先后采用单因子方法和正交实验方法，重复实验确定了最适导入条件：脉冲电压为400V／cm，脉冲次数为5，脉冲宽度为25ms，外源DNA浓度为50mg/L。利用最适导入条件，实现基因元件大规模转移。采用PCR技术对1月龄的原代转基因大菱鲆仔鱼的染色体DNA进行分析，结果表明外源基因的整合率可达28％。     

基于PSR模型的地质遗迹景观脆弱性评价——以永福县为例

地质遗迹景观脆弱性是地质遗迹景观系统对人类活动和自然条件变化反映的重要指标,是评价地质遗迹景观可持续利用的重要指标。文章以永福县典型地质遗迹景观为例,运用地质遗迹景观脆弱性影响因素压力-状态-响应（PSR）模型,从压力、状态、响应3个层面选取17个评价指标,构建地质遗迹景观脆弱性评价指标体系,采用层次分析法（AHP）确定各项指标权重,并运用德尔菲专家咨询法建立5级地质遗迹景观脆弱性划分标准,最后通过综合评分法对永福县地质遗迹景观脆弱性进行评价。结果表明:永福县地质遗迹景观顶子山、小山峡评价值为16.797 0、19.142 1,属微度脆弱;福禄泉、石占溪瀑布评价值为 39.162 8、39.777 5,属低度脆弱;拉孝江、百寿河、板峡湖评价值为 58.546 5、52.330 5、 52.520 9,属中度脆弱;百寿岩、新村屯洞、永福岩评价值为 65.743 0、64.089 3、72.939 5,属高度脆弱,这与实地调查结果相吻合,反映出永福县地质遗迹现状,由此说明PSR模型评价地质遗迹景观脆弱性具有实用性。      

川渝地区喀斯特石林地貌研究

根据川渝地区石林地貌的地质背景、形态类型、成因机制,将其分为太安式、小岩湾式、龙门山式、格萨拉式等4种型式,它们分别发育于下古生界灰岩、二叠系阳新灰岩、石炭系黄龙灰岩、侏罗系-白垩系钙质灰岩屑砾岩中,并分布于不同的气候地貌区.它们分布在海拔1500m以上的高夷平面至海拔400m左右的河谷一级阶地等不同的地貌位置,说明其地貌形成从始新世末-渐新世初开始,经历了多阶段演化,至今仍在发展.这些石林景观都具有独特的科学与美学价值,其中一些已建立了地质公园和相关的保护区.加强对这些石林景观的有效保护与合理利用,具有重要的科学、环境和经济意义.     

双壳贝类CK1基因家族的鉴定及表达分析

酪蛋白激酶1 (CK1)是一种重要的蛋白激酶家族,其在DNA损伤应答和修复、细胞增殖和凋亡、胚胎发育和稳态等重要的生物学过程中都具有复杂多样的调控作用。为了理解CK1基因家族在双壳贝类中的特征、进化及生物学功能,实验采用比较基因组学及生物信息学的方法对双壳贝类CK1基因家族进行了鉴定分析,通过虾夷扇贝高温应激实验,研究了CK1基因家族在高温应激时的表达规律。结果显示,在虾夷扇贝、栉孔扇贝、长牡蛎与侏儒蛤中均存在CK1α,CK1αlike,CK1δ,CK1γ3,并发现CK1ε,CK1γ1,CK1γ2在双壳贝类中发生了丢失。时空表达分析发现,双壳贝类CK1基因均在胚胎发育早期集中表达,并以多细胞/囊胚期为界呈现出母源/合子特异性表达模式。CK1为关键基因的基因共表达模块显著富集在错配修复、核苷酸剪切修复等相关通路上,暗示了CK1基因在双壳贝类胚胎发育过程中参与调控DNA损伤修复过程,从而维持早期胚胎发育时的基因组稳定性。双壳贝类CK1基因在成体中呈现组织特异的表达模式,主要在鳃和雄性性腺中具有相对较高的表达量。虾夷扇贝受到高温应激后,其鳃中PyCK1α, PyCK1αlike和PyCK1δ...     

西南印度洋深海热液硫化物区沉积物微生物群落结构和固氮基因多样性

A sediment sample was collected from a deep-sea hydrothermal vent field located at a depth of 2 951 m on the Southwest Indian Ridge. Phylogenetic analyses were performed on the prokaryotic community using polymerase chain reaction（PCR） amplification of the 16 S rRNA and nifH genes. Within the Archaea, the dominant clones were from marine benthic group E（MBGE） and marine group I（MGI） belonging to the phyla Euryarchaeota and Thaumarchaeota, respectively. More than half of the bacterial clones belonged to the Proteobacteria, and most fell within the Gammaproteobacteria. No epsilonproteobacterial sequence was observed. Additional phyla were detected including the Actinobacteria, Bacteroidetes, Planctomycetes, Acidobacteria, Nitrospirae, Chloroflexi, Chlorobi, Chlamydiae, Verrucomicrobia, and candidate divisions OD1, OP11, WS3 and TM6, confirming their existence in hydrothermal vent environments. The detection of nifH gene suggests that biological nitrogen fixation may occur in the hydrothermal vent field of the Southwest Indian Ridge. Phylogenetic analysis indicated that only Clusters I and III NifH were present. This is consistent with the phylogenetic analysis of the microbial 16 S rRNA genes, indicating that Bacteria play the main role in nitrogen fixation in this hydrothermal vent environment.