弘扬廉政文化 促进廉政建设——全省国土资源系统"廉洁国土"文艺汇演剪影

近年来,我省国土资源系统认真按照省委和省纪委“关于加强廉政文化建设”的要求,紧密结合国土资源改革发展的实际,组织开展了一系列行之有效的活动,丰富了学廉思廉的内容,增强了反腐倡廉教育的渗透力和影响力。在我厅深入开展学习实践科学发展观之际,为充分展示全系统党风廉政建设成果,在省厅的精心筹划和全系统各级党组织的认真准备下,     

文登市加强国土资源文化建设的做法

近年来,文登市国土资源局大力加强国土资源文化建设,不断激发干部活力,提高部门形象力和影响力,极大地促进了国土资源管理工作的开展。1构建和谐进取的精神文化(1)抓理论教育,为精神文化建设奠实基础。坚持把科学发展观的学习作为主题教育的第一讲。每年度下发政治理论学习计划     

大口黑鲈抗菌肽hepcidin cDNA序列和结构分析

以大口黑鲈为材料,提取肝脏总RNA,经RT-PCR扩增出hepcidin cDNA的开放阅读框(ORF)及3′端非编码区序列,应用5′RACE方法得到大口黑鲈hepcidin cDNA5′末端。将所获得的两个片段分别克隆到T载体后进行测序,并拼接成大口黑鲈hepcidin全长cDNA。序列分析表明:大口黑鲈hepcidin全长cDNA为564bp,含有一个258bp的ORF,编码86个氨基酸残基,由信号肽(24个残基)、前肽(42个残基)和成熟肽(20个残基)3部分组成hepcidin前体。在前肽部分具有前肽转化酶典型的RX(K/R)R基元,成熟肽部分含有8个保守的半胱氨酸残基,可形成四个链内二硫桥,使β-折叠结构保持稳定。大口黑鲈Hepcidin与其他鱼类的同源性在29.7%~90.5%间,尤其是信号肽区域,与鳜、尼罗罗非鱼、真鲷、花鲈、黑鯛、金眼狼鲈仅有2~3个氨基酸的差别。     

Cloning and sequence analysis of a full-length cDNA of <Emphasis Type="Italic">SmPP1cb</Emphasis> encoding turbot protein phosphatase 1 beta catalytic subunit

Reversible protein phosphorylation, catalyzed by protein kinases and phosphatases, is an important and versatile mechanism by which eukaryotic cells regulate almost all the signaling processes. Protein phosphatase 1 （PP1） is the first and well-characterized member of the protein serine/threonine phosphatase family. In the present study, a full-length cDNA encoding the beta isoform of the catalytic subunit of protein phosphatase l（PPlcb）, was for the first time isolated and sequenced from the skin tissue of flatfish turbot Scophthalmus maximus, designated SmPPlcb, by the rapid amplification of cDNA ends （RACE） technique. The cDNA sequence of SmPPlcb we obtained contains a 984 bp open reading frame （ORF）, flanked by a complete 39 bp 5＇ untranslated region and 462 bp 3＇ untranslated region. The ORF encodes a putative 327 amino acid protein, and the N-terminal section of this protein is highly acidic, Met-Ala-Glu-Gly-Glu-Leu-Asp-Val-Asp, a common feature for PP1 catalytic subunit but absent in protein phosphatase 2B （PP2B）. And its calculated molecular mass is 37 193 Da and pI 5.8. Sequence analysis indicated that, SmPPlcb is extremely conserved in both amino acid and nucleotide acid levels compared with the PPlcb of other vertebrates and invertebrates, and its Kozak motif contained in the 5＇UTR around ATG start codon is GXXAXXGXXATGG, which is different from mammalian in two positions A6 and G3, indicating the possibility of different initiation of translation in turbot, and also the 3＇UTR of SmPPlcb is highly diverse in the sequence similarity and length compared with other animals, especially zebraf＇lsh. The cloning and sequencing of SmPPlcb gene lays a good foundation for the future work on the biological functions of PP1 in the flatfish turbot.     

Genetic diversity analysis with ISSR PCR on green algae Chlorella vulgaris and Chlorella pyrenoidosa

In the present study, genetic polymorphism and diversity in unicellular clones of Chlorella vulgaris Beijerinck and Chlorella pyrenoidosa Chick were studied with Inter Simple Sequence Repeats PCR (ISSR PCR). Samples including four clones of C. vulgaris and three clones of C. pyrenoidosa were purified by single-clone-choice method. For four C. vulgaris unicellular clones, the total number of the bands scored for 18 primers was 298; and the number of the polymorphic bands was 118, of which 39.6% were polymorphic. The size of PCR products ranged from 200 to 2 500 bp. The total number of bands scored for 18 primers, the number of polymorphic bands and the percentage of three C. pyrenoidosa unicellular clones was 194.83 and 30.8%, respectively. POPGENE analysis show that the average Nei genetic diversity (h *) and Shannon index of diversity (I *) in the four C. vulgaris unicellular clones was 0.2181 and 0.3208, respectively, which is slightly higher than those of the three C. pyrenoidosa unicellular clones (0.190 3 and 0.274 8), which agreed with the percentage of polymorphic bands in the mixed samples of the two species. The results suggest that ISSR is a useful method to Chlorella for intra-species genetic analysis.     

栾川县国土资源局廉政文化建设成效显著

几年来,栾川县国土资源局廉政文化建设常抓不惚,成效显著。自2007年9月份该局被县纪委确定为“廉政文化进机关”工作试点单位后,局党组高度重视,采取全方位、多层次、高标准、深内涵的活动方式,抓好四项工作,实现机关作风五个转变。最近,又被洛阳市纪委、监察局授予市级“廉政文化进机关”示范单位。     

人民生福祉 机关所系

清明前夕,笔者有幸在广州亲聆少壮派国学专家郑赋老师主讲的一堂十分精彩的中国传统文化普及课,课堂上,郑老师屡屡提及的一位圣贤的名字及其事迹令全场的人为之动容,这位圣贤的名字相信大家都不感到陌生,他就是：范仲淹。     

中国生态文化协会常务理事会在京召开

本刊讯(记者王馨凤)2月27日下午,中国生态文化协会常务理事会在北京国际竹藤大厦召开。中国生态文化协会会长、全国政协人口资源环境委员会副主任江泽慧在会上做了题为《弘扬生态文化倡导绿色生活充分发挥     

冲绳日本绒螯蟹线粒体DNA 12S rRNA序列的研究

采集日本冲绳源河川和边野古川的日本绒螯蟹样品 ,参考果蝇与蚤状氵蚤线粒体 DNA12 Sr RNA基因片段序列进行了其相同片段的引物设计、PCR扩增及序列测定。结果表明冲绳两河川 4只日本绒螯蟹的碱基序列完全相同 ,为 4 58bp,其 A、T、G、C含量分别为 16 0 bp(34.94 % )、 179bp(39.0 8% )、4 9bp(10 .70 % )、70 bp(15.2 8% ) ,同果蝇与蚤状氵蚤相同基因片段的序列含量相似。     

浅淡奇石及其文化渊源

介绍了我国奇石观赏的起源和发展进程 ,对我国石文化传统和各发展阶段的石文化特点进行了探讨