真鲷淋巴细胞的分离

用Ficoll-Paque和Percoll液,使用高速离心法对真鲷末梢血中的淋巴细胞进行分离实验.结果指出,用Ficoll-Paque液分离时,2100r/min、20C离心35min,活淋巴细胞数占全部被分离白血球的(79.5±6.5)%.当用Percoll液分离时,在1400r/min、25℃离心30min,淋巴细胞数占全部被分离白血球的(77.2±4.3)%.可见,这2种分离培养液都可用干真鲷淋巴细胞的分离,但在各自的最佳条件下Ficoll-Paque的分离效果略好于Percoll.     

海滨锦葵种子含油量的遗传分析

海滨锦葵(Kosteletzkya virginiva)是重要的能源油料植物,对海滨锦葵种子含油量进行遗传分析具有重要意义.以高油海滨锦葵品种KV23(P1和低油海滨锦葵品种KV16(P2)及其杂交组合的F1、F2群体为材料,以单株种子含油量为指标,应用P1、P2、F1和F2 4个世代的数量性状主基因+多基因混合遗传模型联合分离分析方法,对海滨锦葵种子含油量进行了遗传分析.结果表明,海滨锦葵种子含油量可能由两时加性-显性-上位性主基因+加性-显性多基因共同控制,主基因遗传率为49.87%,主基因和多基因共同解释的遗传率为80.14%.     

欠定盲源分离的稀疏分量分析方法

分析了解决欠定盲源分离问题的稀疏分量分析方法,通过聚类算法得到混合矩阵的估计,通过优化算法恢复源信号。总结了各种实现"聚类"和"优化"的具体方法,论述了欠定盲源分离和稀疏分量分析、压缩传感之间的紧密联系,对欠定盲源分离进行了展望。     

Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas： segregation and null allele identification for linkage analysis

Microsatellites were screened in a backcross family of the Pacific oyster, Crassostrea gigas. Fifteen microsatellite loci were distinguishable and polymorphic with 6 types of allele-combinations. Null alleles were detected in 46.7% of loci, accounting for 11.7% of the total alleles. Four loci did not segregate in Mendelian Ratios. Three linkage groups were identified among 7 of the 15 segregating loci. Fluorescence-based automated capillary electrophoresis (ABI 310 Genetic Analyzer) that used to detect the microsatellite loci, has been proved a fast, precise, and reliable method in microsatellite genotyping.     

Optimization of preparative separation and purification of total polyphenols from Sargassum tenerrimum by column chromatography

Polyphenols from the ethanol extracts of Sargassum tenerrimum (ST) with potent antiallergic effects were studied to optimize separation process through column chromatography. The adsorption and desorption characteristics of three widely used adsorbents: macroporous resin, silica gel, and polyvinylpolypyrrolidone (PVPP), were critically evaluated respectively and studied for the optimization of preparative separation of polyphenols. Static operations on these adsorbents showed that macroporous resin had the best adsorption and desorption capability among the three adsorbents. Dynamic adsorption and desorption with macroporous resin packed column were also conducted to optimize the parameters such as: with the optimal values shown in brackets, the concentration of extract solution (4 times diluted), pH value (6–7), adsorption speed (3 BV h⁻¹, bed volumes/per hour), concentration of ethanol (80%), elution speed (3 BV h⁻¹) and elution volume (7 BV). The chromatographic process so optimized gave a purity of 62.43% from the crude polyphenols, providing a promising basis for large scale preparation of bioactive polyphenols upon further scaling up tests.     

功在国家，利在人民：博大徐光宪

88岁的徐光宪院士,以其学术成就和推动我国稀土加工技术巨大进步,奠定了他在化学界泰斗地位,使我国稀土分离技术走进世界最前列,因此荣膺2008年度中国国家级最高科技奖。     

Optimization of Preparative Separation and Purification of Total PolyphenoJs from Sargassum tenerrimum by Column Chromatography

Polyphenols from the ethanol extracts of Sargassum tenerrimum （ST） with potent antiallergic effects were studied to optimize separation process through column chromatography. The adsorption and desorption characteristics of three widely used adsorbents： macroporous resin, silica gel, and polyvinylpolypyrrolidone （PVPP）, were critically evaluated respectively and studied for the optimization of preparative separation of polyphenols. Static operations on these adsorbents showed that macroporous resin had the best adsorption and desorption capability among the three adsorbents. Dynamic adsorption and desorption with macroporous resin packed column were also conducted to optimize the parameters such as： with the optimal values shown in brackets, the concen- tration of extract solution （4 times diluted）, pH value （6-7）, adsorption speed （3BVh-1, bed volumes/per hour）, concentration of ethanol （80%）, elution speed （3 BV ht） and elution volume （7 BV）. The chromatographic process so optimized gave a purity of 62.43% from the crude polyphenols, providing a promising basis for large scale preparation of bioactive polyphenols upon further scaling up tests.     

地磁内、外源场分离及其强震前内、外源电流体系的演化特征

利用地磁内外源场分离方法,反演1997年11月8日玛尼地震前地下和空间等效电流体系的演化图像,分析了地磁低点位移出现前后等效电流体系变化特征.结果表明:内、外场等效电流体系的变化与地震"低点位移"异常现象有着内在的联系,等效电流体系变化可能是地磁低点位移异常现象产生的原因之一.     

方斑东风螺肿吻症病原菌的分离鉴定及药敏分析

从海南省文昌县某养殖场患病方斑东风螺(Babylonia areolata)的吻、肌肉和肝脏中分离到2株优势菌。人工感染证实其中1株为致病菌,经形态学检测和生化鉴定,该病原菌为鳗弧菌生物变种Ⅰ(Vibrio anguillarum BiovarⅠ)。该病原菌对方斑东风螺的LD50值为1.7×10~6 cfu/g。药敏试验表明派拉西林、罗红霉素、红霉素、洁霉素、氟哌酸、先锋必、妥布霉素、菌必治、复方新诺明、利福平、复达欣、呋喃妥因等12种抗生素类药物对该病原菌敏感。     

天然来源的α-葡萄糖苷酶抑制剂的研究进展

<正>α-葡萄糖苷酶(α-glucosidase,EC3.2.1.20)是一类能够催化碳水化合物非还原末端的α-1,4-糖苷键水解并释放出葡萄糖的酶,包括麦芽糖酶、异麦芽糖酶、蔗糖酶和海藻糖酶等。它在动物体内许多代谢途径中有重要意义,包括糖蛋白和糖脂的合成后加