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新疆沙冬青叶片蛋白质的双向电泳技术研究 总被引:1,自引:1,他引:0
提取新疆沙冬青叶片中蛋白质受到脂类、酚类等次生代谢产物的干扰,针对这种现象,进行了组分提取优化方法和双向电泳技术的改进。实验分析表明,用乙醚淋洗完整叶片,50 mmol·L-1 Tris-HCl缓冲液,上样量240 μg(7 cm胶条),考马斯亮蓝染色等方法可很大程度增加提取可溶性蛋白含量,结合丙酮-三氯乙酸的沉降,可较完整地提取蛋白质组。 相似文献
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Rafeel Riaz 《Astronomische Nachrichten》2024,345(2-3):e230150
Circumstellar disk (CD) around the first generation of stars have been numerically investigated here to see if the cooling regimes also play a role in the formation and evolution of the disk associated with the most massive protostars (MMPS). Also, an emphasis is given to exploring the effect of the initial turbulent motion of the metal-free gas on the resulting morphology of the CD. For this, a systematic range of Mach number has been examined. It has been found that the disk-to-star mass ratio / is larger when the model evolution is based on the first H line cooling followed by subsequent cooling via collision-induced emission than in the model where only H line cooling remains operative. Also, irrespective of the initial turbulence in the cloud, the former type of model yields a CD as massive as 7.66 M while the latter type produces a disk as massive as 1.29 M . Moreover, the inner part of the CD is found dominated by higher subsonic turbulent motions in the case of the former type of models than in the latter type. CDs around MMPS in both types of models show stable disk structures. 相似文献
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