Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of ≈49 kDa size, and weak signals for proteins of ≈41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of ≈56 and 59 kDa size, and also a weak immunoreaction with a protein of ≈43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14–40 ng/g DW in controls to 373–638 ng/g DW in exposed mussels.     

紫菜一新种——福建紫菜的研究

于1978年1月在福建莆田县乐屿岛采集紫菜属标本,进行分类研究。经鉴定表明,其中一种系新种,定名为福建紫菜Porphyra fujianensis sp.nov.。分类方法见曾呈奎等(1962)。本种特征为:藻体长带状,边缘细胞为刺缘型;雌雄同体,精子囊器与果孢子囊成条状或块状,混生或呈镶嵌式分布,形似条斑紫菜;精子囊器分裂式为(?)A_4B_4C_4,果孢子囊分裂式为♀A_2B_4c_2;藻体产生单孢子,进行无性繁殖。模式标本存放于厦门水产学院养殖系。     

溴素的有机化学──Ⅴ.溴代脂肪酸—2,3,5,6—四溴—1,4—苯二酯的合成

以1，4—苯二酚和不同的短链脂肪酸为基本原料，经溴代、加成、形成酰卤和酰化等反应，合成了六种新的溴化脂肪酸—2，3，5，6—四溴—1，4—苯二酯。通过对产物的碳、氢、溴等元素的定量分析，以及红外吸收光谱和氢核磁共振谱的研究，验证了化合物的结构。     

Numerical Simulation of Residual Circulation due to Bottom Rouglmess Variability Under Tidal Flows in A Semi-Enclosed Bay

Nowadays there are some chronic serious environmental problems, such as eutrophication, blue tide and so on, in a complicated coastal zone or a semi-enclosed bay, because the water exchanges between an inner bay and an outer sea is weak compared with the supply of contaminant. Under this situation, a method to improve the water quality by 3-dimensional small unsymmetrical structures has been proposed by Komatsu et al. In this paper, several numerical simulations of the tidal current and concentration for various arrangements of bottom roughness in a semi-enclosed model bay are carfled out with a depth-averaged 2-D numerical model. The model is solved by the hybrid finite analytic method with nonstaggered grid. And the SIMPLES algorithm with Rhie and Chow＇ s momentum interpolation technique is used for the simulation. The effect of Komatsu＇ s method for water purification is examined by numerical simulation. The result of numerical experiment indicates that it is possible to generate a new tidal residual current and to activate a tidal exchange by bottom roughness arrangement only.     

Ecology of the Symbiotic Coral Cladocora caespitosa (L.) (Faviidae, Scleractinia) in the Bay of Piran (Adriatic Sea): II. Energy Budget

Abstract. The temperate, symbiotic coral Cladocora caespitosa occurs naturally with variable numbers of zooxanthellae. This allows us to study differences in the physiology of symbiotic and "non-symbiotic" polyps and to correlate them with zooxanthellae activities.
Zooxanthellae density in "dark" (normal-colored) polyps was 50 times higher than in "white" (bleached) polyps.
The chlorophyll a content per zooxanthella was 3 times, and Chi c was 13 times higher in white polyps than in dark ones. O₂-flux experiments were conducted with colonies in situ and with single polyps - dark and white - under laboratory conditions. Two approaches were used to evaluate the contribution of zooxanthellae translocation products to animal respiration. Both revealed that the animal tissues derive a higher benefit from the zooxanthellae during periods of low water temperature than during warm periods.     

北部湾三维潮流场数值研究(英文)

本文对北部湾三维潮波进行了数值研究,并将所得结果与现有观测资料作了比较。通过比较发现两者相当一致。文中还绘制了M_2和K_1分潮的同潮图,并对其作了讨论。可以断定,北部湾的日潮优势是由日潮共振所引起的。M_2和K_1分潮流的水平分布表明,强流区位于它们各自的蜕化无潮点附近。还讨论了分潮流的铅直结构,可以看出这种结构十分接近实际分潮流场的特征。     

北黄海沉积物——水界面反硝化速率及影响因素研究

探讨近海氮的循环机制,采用乙炔抑制法和现场静态箱法对北黄海夏季局部海域的反硝化速率进行了研究,该海域反硝化速率在2.5~5.8μmol/m2.h之间,平均4.85μmol/m2.h。影响其反硝化速率的主要因素为溶解氧,其次是温度。北黄海的反硝化速率低于珠江口和长江口海域。     

Excess CO2 and pHexcess in the Intermediate Water Layer of the Northwestern Pacific

Excess CO₂ and pH_excess showing an increase in dissolved inorganic carbon and a decrease in pH from the beginning of the industrial epoch (middle of the 19th century) until the present time have been calculated in the intermediate water layer of the northwestern Pacific and the Okhotsk Sea. It is concluded that: (1) The Kuril Basin (Okhotsk Sea) and the Bussol' Strait areas are characterized by the greatest concentrations of excess CO₂ at isopycnal surfaces due to the processes of formation and transformation of intermediate water mass. (2) The largest difference in excess CO₂ concentration between the Okhotsk Sea and the western subarctic Pacific (about 8 µmol/kg) is found at the = 27.0. (3) The difference in excess CO₂ between the western subarctic Pacific and subtropical regions is significant only in the upper part of the intermediate water layer ( = 26.7–27.0). (4) About 10% of the excess CO₂ accumulation in the subtropical north Pacific is determined by water exchange with the subarctic Pacific and the Okhotsk Sea.     

海藻硫酸多糖911对小鼠脾淋巴细胞增殖反应及对细胞产生IL—2作用的影响

本文以~3H—TdR掺入法观察911对小鼠脾淋巴细胞增殖反应的影响并用CTLL细胞检测了其对IL—2的作用。体外实验结果表明,911对小鼠脾淋巴细胞增殖反应有明显的增强作用,以0.5μg/ml的浓度效果最为明显,相对增殖指数RPI可达200％;体内实验则以5mg/kg体重ip,连续7d效果最好,相对增殖指数RPI可达176％;911用药组小鼠IL—2的产生量均高于对照组,以5mg/kg和10mg/kg效果最好。以上实验证明,这一多糖是一种有希望的新免疫调节药物。     

褐牙鲆(♀)、夏鲆(♂)及其杂交子一代线粒体16S rDNA序列遗传特性的初步研究

以线粒体16S rRNA基因部分片段为代表研究雌褐牙鲆Paralichthys olivaceus、雄夏鲆P. dentatus及其杂交子一代间线粒体DNA的遗传特性。母本与父本序列差异较大,属种间差异,在590个位点中有28个变异位点,而且全部为简约信息位点。使用同一对引物扩增亲本与杂交子代,在杂交子代中未检测到父本的线粒体DNA类型,11个处于两种生长阶段的杂交样本仅检测到一种单倍型,而且与母本的一个单倍型为同一种,表明褐牙鲆与夏鲆杂交线粒体DNA遵循母系遗传规律。