发光细菌法在环渤海污水样品遗传毒性检测中的应用

建立了以经过遗传改造的发光细菌Acinetobacter sp.Rec A为受试物种的环境污染物遗传毒性快速检测方法,该方法最快可在3h内得到毒性评价结果。采用该方法评价了环渤海排污口12份污水样品的遗传毒性。环渤海12个排污口的污水样品均表现出了不同水平的遗传毒性,并呈现出一定的分布特征。其中,高毒水质集中出现在山东半岛污水样品中,中毒水质集中出现在辽东半岛地区,而低毒水质主要集中于京津冀地区。综上,在环渤海的污水样品遗传毒性检测中,这种新型发光细菌法具有快速、灵敏、简便等优点,可为以后近海环境的水质生物毒性检测提供参考依据,具有应用于近海环境水质快速监测与评价的潜力。     

COOL AND FRESHWATER SKIN OF THE OCEAN DURING RAINFALL

Rainfall over the sea modifies the molecular boundary layers of the upper ocean through a variety of different effects. These cover the freshwater flux stabilizing the near-surface layer, additional heat flux established due to rain versus surface temperature differences, modification of physical parameters by temperature and salinity changes, enhancement of the surface roughness, damping of short gravity waves, surface mixing by rain, and transfer of additional momentum from air to sea. They are separately described and included in our surface renewal model to investigate the rain's influence on the cool skin of the ocean and the creation of a haline molecular diffusion layer. Simulations with the upgraded model show that the most important effect on the conductive layer is that of reduced renewal periods followed by additional surface cooling due to rain on the order of 0.1 K. At rain rates below 50 mm h-1 rainfall is not able to completely destroy the mean temperature difference across the cool skin. A freshwater skin is created that exhibits a salinity difference exceeding 4 under strong rainfall. Comparisons with field data of the cool skin taken during the Coupled Ocean Atmosphere Response Experiment confirm the upgraded renewal model. Surface salinity measurements taken during the same field campaign are consistent with the calculated salinity differences across the freshwater skin. The enhancement of surface roughness by natural rain is less pronounced than described in earlier laboratory studies of rain with large drop sizes only.     

Semi-automated bathymetric spectral decomposition delineates the impact of mass wasting on the morphological evolution of the continental slope,offshore Israel

Understanding continental-slope morphological evolution is essential for predicting basin deposition. However, separating the imprints and chronology of different seafloor shaping processes is difficult. This study explores the utility of bathymetric spectral decomposition for separating and characterizing the variety of interleaved seafloor imprints of mass wasting, and clarifying their role in the morphological evolution of the southeastern Mediterranean Sea passive-margin slope. Bathymetric spectral decomposition, integrated with interpretation of seismic profiles, highlights the long-term shape of the slope and separates the observed mass transport elements into several genetic groups: (1) a series of ~25 km wide, now-buried slide scars and lobes; (2) slope-parallel bathymetric scarps representing shallow faults; (3) slope-perpendicular, open slope slide scars; (4) bathymetric roughness representing debris lobes; (5) slope-confined gullies. Our results provide a multi-scale view of the interplay between sediment transport, mass transport and shallow faulting in the evolution of the slope morphology. The base of the slope and focused disturbances are controlled by ~1 km deep salt retreat, and mimic the Messinian base of slope. The top of the open-slope is delimited by faults, accommodating internal collapse of the margin. The now-buried slides were slope-confined and presumably cohesive, and mostly nucleated along the upper-slope faults. Sediment accumulations, infilling the now-buried scars, generated more recent open-slope slides. These latter slides transported ~10 km³ of sediments, depositing a significant fraction (~3 m in average) of the sediments along the base of the studied slope during the past < 50 ka. South to north decrease in the volume of the open-slope slides highlight their role in counterbalancing the northwards diminishing sediment supply and helping to maintain a long-term steady-state bathymetric profile. The latest phase slope-confined gullies were presumably created by channelling of bottom currents into slide-scar depressions, possibly establishing incipient canyon headword erosion.     

关于可控源音频大地电磁法探测深度的探讨

分析了水平电偶极子源均匀半空间电磁场表达式特征,进而推广到双极源电磁场。基于双极源一维模拟结果,研究了可控源音频大地电磁法探测深度与频率、电距、收发距、覆盖层电阻率的关系。结果表明:在保证一定信噪比的前提下,决定可控源音频大地电磁法最大探测深度的是收发距和电阻率综合作用下进入过渡区及近区场时对应的频率,并不是简单的频率越小,探测深度越大。进入近区场,可控源音频大地电磁法不再具有测深能力。最后统计得到电磁场由远区进入过渡区时的|kr|值约为4.789,由过渡区进入近区时的|kr|值约为0.106,进而得到卡尼亚视电阻率的最大探测深度约为0.3倍的收发距,全区视电阻率的最大探测深度为3.7倍的收发距。在某多金属矿上的勘探实例印证了上述认识。     

http://www.sciencedirect.com/science/article/pii/S1674987111001277

A numerical model is developed for investigating the evolution of fracture permeability in a coupled fracture-matrix system in the presence of fracture-skin with simultaneous colloidal and bacterial tr...     

16S rRNA gene phylogenesis of culturable predominant bacteria from diseased Apostichopus japonicus (Holothuroidea, Echinodermata)

Cultured Apostichopus japonicus in China suffers from a kind of skin ulceration disease that has caused severe economic loss in recent years. The disease, pathogens of which are supposed to be bacteria by most researchers, is highly infectious and can often cause all individuals in the same culture pool to die in a very short time. The 16S rRNA gene phylogenesis of the culturable bacteria from the lesions of diseased individuals was conducted to study the biodiversity of the bacterial communities in the lesions and to identify probable pathogen(s) associated with this kind of disease. S. japonica samples were selected from a hatchery located in the eastern part of Qingdao, China. Bacterial universal primers GM5F and DS907R were used to amplify the 16S rRNA gene of bacteria colonies, and touchdown PCR was performed to amplify the target sequences. The results suggest that γ- proteobacteria (Alteromonadales and Vibrionales) of CFB group, many strains of which have been also determined as pathogens in other marine species, are the predominant bacterial genera of the diseased Apostichopus japonicus individuals.     

青岛近海表皮温度和表层温度之差的观测及模糊数学分析

１９９３．１１．２３．９：００－１１．２８．９：００随“东方红”号海洋考察船,采用“走航式海面遥感参数自动观测系统”在青岛近海进行了５个昼夜的连续观测。根据所得资料,首先对表皮水曙和表层水温之差与各因子之间的关系进行了分析,然后利用数学方法对所获数据进行了计算。结果表明,海洋表皮水温与表层水温之差和海面风速,气温与水温之差及太阳辐射等均有明显关系。但此关系难以用传统数学方法准确表述。应用模糊推理方     

桩顶荷载对负摩阻力性状影响的现场试验

负摩阻力是桩周土体沉降产生的桩附加沉降和下拽力的综合效应。为了研究不同的桩顶荷载对负摩阻力基桩性状的影响,在同一个试验场地上,对不同桩顶荷载作用下完全相同的3根桩的负摩阻力性状进行了现场测试。测试结果表明,桩顶荷载对于桩的负摩阻力性状有明显影响。随着桩顶荷载的增加,负摩阻力引起的桩附加沉降越大,桩周土体沉降引起的负摩阻力就越小;负摩阻力引起的下拽力也随着桩顶荷载的增加而减小;中性点位置随着桩顶荷载的增加而上升。这对桩基负摩阻力特性的研究是有益的。     

The eco-nutrition requirements for dietary protein and its rhomb characteristics in juvenile turbot (Scophthalmus maximus L.)

We evaluated the dietary protein requirements of juvenile turbot (Scophthalmus maximus L.) and their effects on aquatic quality. Five experimental diets were formulated containing 450, 480, 500, 520, and 540 g/kg. Each diet was randomly assigned to triplicate groups of juvenile turbot (mean initial body weight 34.5 ± 5.5 g) for 88 d. Both the weight gain ratio and feed efficiency increased with increasing dietary protein up to 500 g/kg, but no further improvement was detected when dietary protein levels were >500 g/kg. Protein intake and digestion increased with protein levels, while fecal nitrogen and nitrogen content in seawater increased only when dietary protein exceeded 500 g/kg. Protein digestibility was highest at intermediate dietary protein levels. Chemical oxygen demand, nitrite-nitrogen (NO2--N) and phosphatic-phosphor (PO43--P) levels increased in the rearing water as dietary protein levels increased. The optimum eco-nutrition level of dietary protein for juvenile turbot was 500 g/kg under the current experimental conditions. The diets containing 540 and 500 g/kg protein had similar growth rates and feed conversion ratios, but levels of ammonia (NH4+) and nitrogen were considerably higher in the water and feces, respectively, at the higher level of dietary protein. The difference in the pattern of change between body weight gain and ammonia concentration in water with increasing dietary protein is described by rhomb characteristics.     

Effect of stocking density on performances of juvenile turbot (Scophthalmus maximus) in recirculating aquaculture systems

Limited information has been available about the influence of loading density on the performances of Scophthalmus maximus, especially in recirculating aquaculture systems (RAS). In this study, turbot (13.84±2.74 g; average weight±SD) were reared at four different initial densities (low 0.66, medium 1.26, sub-high 2.56, high 4.00 kg/m²) for 10 weeks in RAS at 23±1°C. Final densities were 4.67, 7.25, 14.16, and 17.47 kg/m², respectively, which translate to 82, 108, 214, and 282 percent coverage of the tank bottom. Density had both negative and independent impacts on growth. The final mean weight, specific growth rate (SGR), and voluntary feed intake significantly decreased and the coefficient of variation (CV) of final body weight increased with increase in stocking density. The medium and sub-high density groups did not differ significantly in SGR, mean weight, CV, food conversion rate (FCR), feed intake, blood parameters, and digestive enzymes. The protease activities of the digestive tract at pH 7, 8.5, 9, and 10 were significantly higher for the highest density group, but tended to be lower (not significantly) at pH 4 and 8.5 for the lowest density group. The intensity of protease activity was inversely related to feed intake at the different densities. Catalase activity was higher (but not significantly) at the highest density, perhaps because high density started to induce an oxidative effect in turbot. In conclusion, turbot can be cultured in RAS at a density of less than 17.47 kg/m². With good water quality and no feed limitation, initial density between 1.26 and 2.56 kg/m² (final: 7.25 and 14.16 kg/m²) would not negatively affect the turbot cultured in RAS. For culture at higher density, multi-level feeding devices are suggested to ease feeding competition.