螺旋藻多糖对肿瘤细胞生长及Hela细胞早期凋亡作用的实验研究

采用MTT法(四甲基偶氮唑盐比色法)研究了钝顶螺旋藻多糖(PSP)对Hela细胞及HepG2细胞生长的抑制作用。结果表明,随PSP浓度及培养时间的增加,肿瘤细胞存活率逐渐降低,抑制率逐渐增加,以PSP40 mg/L作用72 h最为显著。应用Annexin V/PI双染色流式细胞仪检测了早期Hela细胞凋亡,未经PSP处理的正常Hela细胞凋亡细胞极少,经PSP处理的细胞,凋亡细胞的百分比明显高于正常对照组,其作用随着剂量的增加和时间的延长而增强,具有量效关系和时效关系。结果说明PSP的抗肿瘤机制,除了诱导肿瘤细胞凋亡,还存在细胞毒性等其他机制,是多种机制共同作用的结果。     

三峡大坝会减小东海的上升流和生产力吗?——敬复王保栋、孙霞两位指教

2000年笔者利用很简单的一维稳态箱式模型,指出若三峡大坝减少了流入东海的淡水量,则将减少东海陆棚边缘涌升的黑潮次表层水.由于黑潮次表层水饱含营养盐,因此若涌升速率降低了,涌升至东海大陆架的营养盐也就跟着降低,从而影响生物生产力及渔获.王、孙二位提出了不同的看法,并正确地指出了一维稳态箱式模型过度简化所导致的若干问题.然而,王、孙二位似乎对大陆架上之浮力效应原理有所误解,因而误以为浮力效应原理与箱式模型所得推论相反.     

URANS investigation of the interaction between the free surface and a shallowly submerged underwater vehicle at steady drift

An axisymmetric underwater vehicle (UV) at a steady drift angle experiences the complex three-dimensional crossflow separation. This separation arises from the unfavorable circumferential pressure gradient developed from the windward side toward the leeward side. As is well known, the separated flow in the leeward side gives rise to the formation of a pair of vortices, which affects considerably the forces and moments acting on the UV. In this regard, the main purpose of the present study is to evaluate the role of the leeward vortical flow structure in the hydrodynamic behavior of a shallowly submerged UV at a moderate drift angle traveling beneath the free surface. Accordingly, the static drift tests are performed on the SUBOFF UV model using URANS equations coupled with a Reynolds stress turbulence model. The simulations are carried out in the commercial code STARCCM+ at a constant advance velocity based on Froude number equal to F_n = 0.512 over submergence depths and drift angles ranging from h = 1.1D to h = ∞ and from β = 0 to β = 18.11°, respectively. The validation of the numerical model is partially conducted by using the existing experimental data of the forces and moment acting on the totally submerged bare hull model. Significant interaction between the low-pressure region created by the leeward vortical flow structure and the free surface is observed. As a result of this interaction, the leeward vortical flow structure appears to be largely responsible for the behavior of the forces and moments exerted on a shallowly submerged UV at steady drift.     

Quality control methods for KOOS operational sea surface temperature products

Sea surface temperature SST obtained from the initial version of the Korea Operational Oceanographic System(KOOS) SST satellite have low accuracy during summer and daytime. This is attributed to the diurnal warming effect. Error estimation of SST data must be carried out to use the real-time forecasting numerical model of the KOOS. This study suggests two quality control methods for the KOOS SST system. To minimize the diurnal warming effect, SSTs of areas where wind speed is higher than 5 m/s were used. Depending on the wind threshold value, KOOS SST data for August 2014 were reduced by 0.15°C. Errors in SST data are considered to be a combination of random, sampling, and bias errors. To estimate bias error, the standard deviation of bias between KOOS SSTs and climatology SSTs were used. KOOS SST data yielded an analysis error standard deviation value similar to OSTIA and NOAA NCDC(OISST) data. The KOOS SST shows lower random and sampling errors with increasing number of observations using six satellite datasets. In further studies, the proposed quality control methods for the KOOS SST system will be applied through more long-term case studies and comparisons with other SST systems.     

考虑发电机尾流作用的潮流能理论可开发量的评估

In this study, we construct one 2–dimensional tidal simulation, using an unstructured Finite Volume Coastal Ocean Model(FVCOM). In the 2–D model, we simulated the tidal turbines through adding additional bottom drag in the element where the tidal turbines reside. The additional bottom drag was calculated from the relationship of the bottom friction dissipation and the rated rotor efficiency of the tidal energy turbine. This study analyzed the effect of the tidal energy turbine to the hydrodynamic environment, and calculated the amount of the extractable tidal energy resource at the Guishan Hangmen Channel, considering the rotor wake effect.     

0917号台风“芭玛”折向东南向移动原因分析

利用NCEP全球数据同化系统(GDAS)1°×1°分析资料,对0917号台风"芭玛"折向东南向移动原因进行了分析。结果表明,中高纬度环流调整是"芭玛"折向东南向移动的根本原因;0918号台风"茉莉"通过改变外围环境场的强度、形状对"芭玛"台风产生间接影响,而两台风之间逆时针互旋以及台风"茉莉"外围强大的环流对台风"芭玛"的直接作用是台风"芭玛"折向东南向移动的关键。对台风"芭玛"经纬向UV最大风速变化诊断分析表明,"芭玛"经纬向UV最大风速中心的转移对"芭玛"折向东南向移动有重要影响,经纬向UV最大风速差的变化对"芭玛"转向具有预示作用,经纬向最大风速差的合成风方向与台风中心未来移动方向有一定的关系。     

不同刚度植物杆群对规则波传播及紊动特性影响研究

以不同刚度硅胶圆杆群为概化植物模型,测定其抗弯弹性模量,通过波浪水槽实验,研究规则波在不同刚度植物杆群内的流速分布、紊动特征及不同刚度杆群的消浪效果。实验结果表明,当波浪通过柔性杆群时,受其摆动的影响,流速周期变化从单峰型逐渐转变成双峰型,杆群刚度越小形成的二次波峰越明显;不同刚度杆群内水体紊动强度变化显示,杆群刚度越大,造成杆群内水体的紊动强度越大;随着杆群抗弯弹性模量的增大,其消浪系数也增大,消浪系数的增长与材料的抗弯弹性模量值非线性关系,而是在某一弹性模量范围内,对消浪系数的影响较为敏感。     

吡啶硫酮类防污剂对华美盘管虫早期不同发育阶段的毒性效应研究

以中国南海常见的多毛类——华美盘管虫(Hydroides elegans)作为受试生物,分别研究了吡啶硫酮铜和吡啶硫酮锌对早期不同发育阶段的华美盘管虫的急性毒理效应。发现暴露于吡啶硫酮铜的华美盘管虫精子和卵子的受精半数抑制浓度(IC50)分别为19.49μg/L和88.44μg/L,担轮幼虫24 h半数致死浓度(24h-LC50)和48 h半数致死浓度(48h-LC50)分别为7.35μg/L和5.00μg/L,后担轮幼虫24h-LC50和48h-LC50分别为8.57μg/L和5.87μg/L;暴露于吡啶硫酮锌的华美盘管虫精子和卵子的受精IC50分别为36.74μg/L和159.59μg/L,担轮幼虫24 h-LC50和48 h-LC50分别为8.57μg/L和6.67μg/L,后担轮幼虫24 h-LC50和48 h-LC50分别为12.03μg/L和8.07μg/L。表明华美盘管虫早期发育的不同阶段对吡啶硫酮铜和吡啶硫酮锌的敏感性表现出一定差异,与配子受精率和后担轮幼虫的毒性反应相比,担轮幼虫对吡啶硫酮铜和吡啶硫酮锌的毒性表现出较高的敏感性;而且,对吡啶硫酮铜的敏感性普遍高于吡啶硫酮锌。     

中国北方砂栖纤毛虫的空间分布及其驱动因素

为深入了解不同地理尺度因素对驱动微型生物群落空间结构的相对影响,本研究于2020年10月调查了中国北方14个砂质潮间带的砂栖纤毛虫群落。研究结果如下：（1）检获纤毛虫105种,隶属26目65属,按丰度优势依次为帆口目、小胸目和环毛目;（2）黄海、渤海两区域的环境因子存在显著差异,但纤毛虫群落组成趋于相似;（3）偏Mental分析显示,环境条件较空间距离在纤毛虫群落组成的驱动中更为重要,其中盐度、粒度和海滩坡度是解释纤毛虫空间分布的最优环境因子组合,而潮差和溶解无机氮含量是次要影响因子;（4）沿海区域微型生物的强扩散效应在一定程度上掩盖了环境异质性影响。综上,由于沿海生态系统中微型生物受到更少扩散限制,在空间分布格局的形成上,环境条件的影响比空间距离更重要。本研究为海洋微型生物地理学提供了基础数据,有助于在全球变化背景下制定沙滩管理和保护规划。     

The 2020/21 Extremely Cold Winter in China Influenced by the Synergistic Effect of La Niña and Warm Arctic

In the first half of winter 2020/21,China has experienced an extremely cold period across both northern and southern regions,with record-breaking low temperatures set in many stations of China.Meanwhile,a moderate La Ni?a event which exceeded both oceanic and atmospheric thresholds began in August 2020 and in a few months developed into its mature phase,just prior to the 2020/21 winter.In this report,the mid?high-latitude large-scale atmospheric circulation anomalies in the Northern Hemisphere,which were forced by the negative phase of Arctic Oscillation,a strengthened Siberian High,an intensified Ural High and a deepened East Asian Trough,are considered to be the direct reason for the frequent cold surges in winter 2020/21.At the same time,the synergistic effect of the warm Arctic and the cold tropical Pacific(La Ni?a)provided an indispensable background,at a hemispheric scale,to intensify the atmospheric circulation anomalies in middle-to-high latitudes.In the end,a most recent La Ni?a prediction is provided and the on-coming evolution of climate is discussed for the remaining part of the 2020/21 winter for the purpose of future decision-making and early warning.