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Molecular identification of bloom-forming species Phaeocystis globosa(Prymnesiophyta)and its dispersal based on rDNA ITS sequence analysis 总被引:3,自引:1,他引:3
Abstract-The colony-forming Phaeocystis species are causative agents of dense bloom occurrences incoastal waters worldwide. It is difficult to separate them because of the different morphologies associatedwith their colonial stages. In this study we applied molecular approaches to analyze the genetic variationof Phaeocystis globosa and Phaeocystis pouchetii from several geogrtaphic regions, and to assist in tracingthe dispersal of bloom-forming Phaeocystis species in coastal waters of China. The Sequences of the inter-nal transcribed sacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystisstrains were determined. Sequence comparison shows that P.globosa was the most divergent toP. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences ex-isted between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearlyshows that the species isolated from the southeast coast of China is identified as P. globosa rather thanP. cf. po 相似文献
74.
山东半岛东部诸岛水域叶绿素—a含量和初级生产力 总被引:1,自引:0,他引:1
据1990.11-1991年8月期间,山东半岛东部诸岛水域调查资料,分析了该水域叶绿素-a含量的时空分布和初级生产力的分布与变化,此分布与该水域的温度和营养盐水含量密切相关。叶绿素-a含量的季节变化分三种类型,年变幅为0.11-12.81mg/m^3,年平均值为1.17mg/m^3。初级生产力夏季〉春季〉秋季〉冬季,年变幅为23.00-791.60mg.c/m^2.d年平均为152.0mg.c/m 相似文献
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Abstract. Four sites were sampled in kelp (Macrocysiis pyrifera) forests occupying rocky bottom habitats along a wave exposure gradient in central California. Consistent betwecn-site differences were found in the three major structural elements - the surface canopy, the undcrstory assemblage, and the ground cover/turf assemblage - of kelp forest communities. Macrocysiis pyrifera was found at all four sites. Nereucyslis tuelkeana only at the most exposed site. The understory kelps Laminaria setchellii and Pterygopltora californica were also characteristic of exposed sites. Articulated coralline algae were more abundant at exposed sites than protected, while fleshy red algae showed the opposite pattern. All four study sites were located along 8.5 km of coastline, and thus were assumed to have available to them the same species pool for colonization. The substrate composition was the same and the amount of unconsolidated substrate was similar at all four sites. We suggest that exposure to wave-generated water motion, through its influence on the surface canopy and therefore on the amount of light reaching the bottom, is responsible for these between-site differences. 相似文献
76.
Sequence variation of the first internal transcribed spacer of ribosomal DNA ( ITS - 1 ) was examined and its application to the study of genetic variation was explored in four populations of farter' s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA (analysis of molecular variance) showed that the majority (96.26%) of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 ( = 0.042), indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly. 相似文献
77.
由于海岸带的地理条件和海洋动力特性的复杂性以及人类开发活动对海岸带的影响,近岸海域的生态环境系统在空间分布上有很大的变异性.基于现场实测资料,建立系统的空间变异分析模型,对海岸带生态环境空间变化规律进行分析,可以充分了解不同区域的污染特征和变化趋势.应用所构建的空间变异分析模型对渤海湾天津近岸海域的水环境特性进行分析,结合对应年份的入海流量及其变化,分析了天津渤海湾海岸带污染的主因子和区域水环境的分布特征,结果可以对海洋功能区的划分和水环境综合评价结果进行验证和补充. 相似文献
78.
79.
吕宋海峡输送年际变异数值研究 总被引:1,自引:0,他引:1
利用MITgcm数值模式1980—2001年的常规模拟输出数据,研究了吕宋海峡22年平均的体积、热量和盐量输送随深度的变化,并利用小波变换分别分析了吕宋海峡3个层次(0—145、145—915、915—1 837m)体积和热量输送的年际变化,主要结果如下。(1)在吕宋海峡1 837m层以下,仍有一定数量的西北太平洋海水(体积和盐)西向输送到南海;(2)915—1 615m层的年际体积输送的变化趋势与上两个层次(0—145m、145—915m)类似,但是在某些ENSO时期(如1985、1987、1991—1995年),其年际变化与145—915m层年际变化的位相相反;(3)吕宋海峡年际体积(热量)输送的小波谱图较明显地反映出ENSO现象对吕宋海峡不同层次的输送均产生一定影响。145—915m层次的吕宋海峡年际体积输送与ENSO现象相关性显著。 相似文献
80.
1 .IntroductionTheglobalairtemperatureroseabout 0 .5~ 0 .6°Coverthepast 2 0thcentury ,andtheglobalmeansealevelincreasedbyabout2 0cmduringtheperiod .Theregionalmeansealevelriseswiththerisingglobalmeansealevel.Zuoetal.( 1 997)indicatedthatthemeanrisingrateofabsolutemeansealevelalongtheChinacoastontheassumptionofunifiedisostaticdatumis 2mm a .Woodworth( 1 999)analyzedsealevelspanning 1 76 8tothepresentinLiverpool,andobtainedaseculartrendforheperiodupto 1 880of0 .39± 0 .1 7mm a ,andatrendfort… 相似文献