孑遗植物四合木(Tetraena mongolica)异地保护条件下的气候生物学特征与光合效率

为了廓清异地保护条件下孑遗濒危植物四合木(Tetraena mongolica)的气候生物学特征、光合生理生态适应性及生境适宜性,应用1971—2011年的地面气象资料进行了不同试验区的气候生物图解与分析,测定了四合木及其伴生种白刺(Nitraria tangutorum)的叶片气体交换参数及光合效率。结果表明:①四合木自然分布区(内蒙古乌海地区)与异地保护区的气候生物学特征存在明显分异;②当年种植的四合木实生苗的生长量大小依次为:乌海四合木核心区实生苗鄂尔多斯实生苗阿拉善实生苗;③自然条件下,5个处理的净光合速率(Pn)依次为阿拉善移植的四合木成株(24.05±1.68μmol·m-2·s-1)乌海客土栽培的四合木(19.97±1.05μmol·m-2·s-1)乌海栽培的四合木实生苗(18.96±1.04μmol·m-2·s-1)鄂尔多斯栽培的四合木实生苗(16.64±0.92μmol·m-2·s-1)阿拉善栽培的四合木实生苗(16.48±0.13μmol·m-2·s-1);异地保护试验区栽培的四合木实生苗的Pn没有明显差异(p≥0.05),即原生境条件下栽培的四合木实生苗的Pn较高。同时,异地保护栽培四合木实生苗中,鄂尔多斯栽培的四合木实生苗的Pn阿拉善栽培的四合木实生苗。从四合木光合作用特征中的Pn、蒸腾速率、气孔导度和胞间CO2浓度、气候生物学特征和生长量等综合研究结果可以推断,从原生境区东移的实生苗迁地保护,使四合木具有更高的生理生态适宜性和环境适应性。     

米槠次生林内4种植物叶片DOM的数量和质量特征

选取福建省三明市米槠次生林内的4种植物,即米槠(CAC),木荷(SCS),山杜英(ELS),芒萁(DID)为研究对象,对其叶片进行了室内淋溶处理,并分析了淋溶液中的可溶性有机碳(DOC)和可溶性有机氮(DON)含量及pH值,芳香性指标(AI)和荧光效率指数(Feff)等,以揭示其淋溶液中可溶性有机质(DOM)的数量和质量特征。结果表明:4种植物叶片DOM含量总体随淋溶次数的增加逐渐减小,其中首次淋溶后淋溶液中的DOC和DON含量最大,分别占总淋溶量的平均值为67.90%和44.51%;淋溶液中前3次DOC和DON淋溶量总和分别占10次淋溶总量的87.40%~94.77%和68.46%~72.49%;且前3次淋溶次数间呈极显著差异(P0.01),其后差异性不显著。DOM的pH值和光谱指标随着淋溶次数的增加逐渐上升.总体上乔木树种DOM的数量和质量比林下植被要高,这是由于乔木树种的DOM含有更多的养分和芳香类和腐殖化的高分子量化合物,而林下植被的DOM含有较多低分子量化合物和易分解组分。     

基于双视角竞争态与亲景度中国入境旅游客源市场分析

利用市场竞争态与亲景度构建综合评价模型,分析1998~2000年和2009~2011年两个时段中国入境旅游客源市场及其变化。结果发现：（1）1998~2000年美国等为明星市场,日本为金牛市场,印度等为幼童市场,英国等为瘦狗市场。（2）2009~2011年新加坡等为明星市场,韩国等为金牛市场,印度等为幼童市场,瑞典等为瘦狗市场。（3）受空间距离的影响,强亲景市场比较稳定,弱亲景、弱疏景、强疏景3种市场则有可能发生较大变化。（4）经过10年的发展明星市场和幼童市场数量保持不变,金牛市场增加到3个,瘦狗市场减少到3个,客源市场逐步壮大,入境旅游市场发展良好。     

太平洋鳕鱼脑中硫苷脂的分离纯化和分析

以太平洋鳕鱼为原料,从鳕鱼脑中分离硫苷脂,确立了提取及纯化的条件,并对硫苷脂的纯度和分子种进行分析。首先,采用氯仿/甲醇(2∶1,v/v)提取总脂。然后,依次采用氯仿/甲醇/水(7∶3∶0.3,v/v/v)洗脱硅胶柱层析,含0.2mol/L乙酸铵的氯仿/甲醇/水(30∶60∶8,v/v/v)洗脱DEAE Sephadex-A25离子交换柱层析,40%甲醇脱盐和100%甲醇洗脱反相C8柱层析,获得硫苷脂纯品。最后,利用500YMC Diol液相色谱柱(3.0mm×250mm,5μm)分离,以正己烷/异丙醇(70∶30,v/v),异丙醇/水/甲酸/氨水(100∶13∶1∶0.14,v/v/v/v)为流动相梯度洗脱,采用负电喷雾电离(ESI)和母离子扫描模式,用液相色谱-串联质谱(LC-MS/MS)法分析了鳕鱼脑硫苷脂的纯度和分子种组成,并比较了其与哺乳动物脑中硫苷脂分子种组成的异同。结果表明,本实验制得的鳕鱼脑硫苷脂纯度为90.74%,与哺乳动物类似,鳕鱼脑硫苷脂的长链碱基以鞘氨醇为主,主要分子种为d18∶1-C24∶1,但脂肪酸的羟基化程度略低,且含少量独特的分子种,如d18∶1-22∶1和d18∶2-25∶2。     

Eualus heterodactylus sp. nov., a new hippolytid shrimp from Chinese coast of the Yellow Sea （Crustacea,Decapoda, Caridea）

A new species from the caridean family Hippolytidae, Eualus heterodactylus sp. nov. is described and illustrated based on the specimens collected from Chinese coast of the Yellow Sea. The new species is a part of an informal species group characterized by the possession of epipods on the anterior three pairs of pereopods, and is distinguished from other species of this group by the dactyli of the third to fifth pereopods possessing distinctly stair-like flexor margins in males.     

基于cox1条形码的鱼肚DNA分子鉴定

为鉴定鱼肚的正品来源,运用一对特异性引物扩增并测定10 个不同价格（700～1 500 元/kg）鱼肚样本的线粒体DNA 细胞色素c 氧化酶Ⅰ（cox1）约660 bp 的序列.通过BLASTN 比对DNA 序列同源度,判定鱼肚所属种类分别为鲈形目石首鱼科（Sciaenidae）、马鲅科（Eleutheronema）、笛鲷科（Lutjanidae）、带鱼科（Trichiuridae）.cox1 条码序列获取便捷,基于此条码序列的物种鉴定技术可用于鱼肚种类鉴别.     

Identification of five sea cucumber species through PCR-RFLP analysis

Sea cucumbers are traditional marine food and Chinese medicine in Asia. The rapid expansion of sea cucumber market has resulted in various problems, such as commercial fraud and mislabeling. Conventionally, sea cucumber species could be distinguished by their morphological and anatomical characteristics; however, their identification becomes difficult when they are processed. The aim of this study was to develop a new convenient method of identifying and distinguishing sea cucumber species. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of mitochondrial cytochrome oxidase I gene (COI) was used to identifing five sea cucumber species (Apostichopus japonicus, Cucumaria frondosa, Thelenota ananas, Parastichopus californicus and Actinopyga lecanora). A 692 bp fragment of COI was searched for BamHI, KpnI, PstI, XbaI and Eco31I restriction sites with DNAMAN 6.0, which were then used to PCR-RFLP analysis. These five sea cucumber species can be discriminated from mixed sea cucumbers. The developed PCR-RFLP assay will facilitate the identification of sea cucumbers, making their source tracing and quality controlling feasible.     

Effects of substratum type on fish assemblages in shallow areas of a tropical estuary

While there is already a comprehensive understanding of the effects of environmental variables, such as dissolved oxygen, temperature and salinity, on the structure, biomass and metabolism of aquatic biota in estuarine habitats, the effect of sedimentation, a harmful underlying factor, remains unclear. The aim of this study was to assess the differences in fish assemblages along the freshwater to salt water gradient of a large tropical estuary, and to evaluate the effects on them of habitat disturbance associated with shallow water sedimentation in the intertidal areas. Fish and environmental variables were recorded in the upper, middle and lower salinity zones of the estuary at ebb tide in both the dry and wet seasons. Sediment samples associated with different levels of habitat disturbance were analysed using granulometry, and their organic content and dissolved oxygen levels were quantified. Water temperature, salinity, pH and dissolved oxygen levels were also measured. Habitat disturbance was found to be correlated with the density, biomass and richness of fish assemblages. A total of 77 species were recorded, forming two distinct fish assemblages, with the Eleotridae family dominating in the upper zone, and Gerreidae, Gobiidae and Tetraodontidae the most common in the middle and lower estuary. Changes in the structure of fish assemblages, including reductions in density, biomass and richness, were associated with disturbance to natural features, where muddy sediment was replaced by sandy sediment and the quantity of organic matter fell. Atherinella brasiliensis was the species which showed a preference for the disturbed areas in the middle and lower zones, while Dormitator maculatus showed a preference for them in the upper estuary. They may be taken as indicators of habitat disturbance due to shallow sedimentation.