基于GTP的地下工程与围岩一体化真三维空间构模

针对地下工程开挖体的基本特征及其真三维建模要求，在广义三棱柱(GTP)模型的基础上进行扩展，提出采用E—GTP模型来构建地下工程开挖体的真三维模型。通过与其围岩地质体的G—GTP模型的耦合，实现了基于GTP的工程开挖体与围岩地质体的一体化建模。该模型将地下三维空间实体抽象为点、线、面、体四类对象，通过结点、TIN边、侧边、TIN面、侧面和GTP体元共6个基本几何元素进行统一表达。该文重点讨论了E—GTP的建模原理与方法，并介绍了基于面向对象思想的一体化空间数据结构。     

从地理信息系统到虚拟地理环境的认知转变

自20世纪60年代初以来,地理信息系统引领了地理信息的分析与服务。然而,由于地理学家对于地理过程开展多维模拟的需求,同时许多大型工程需要对于不同方案的后果事先开展模拟评估,为此中国学者们在世纪相交之际开始了对于虚拟地理环境的探索。本文从地理信息系统到虚拟地理环境的认识上的转变着手,分析了虚拟地理环境的演进过程、当前的阶段定位与挑战,着重探讨了虚拟地理环境研究的新态势：基于虚拟地理环境的地理空间认知研究、虚拟地理环境与实验地理学新方向、大数据背景下的虚拟地理认知实验方法和虚拟地理环境与地理知识工程,并给出最新研究思路。     

Application of Linear Mean-Square Estimation in Ocean Engineering

The attempt to obtain long-term observed data around some sea areas we concern is usually very hard or even impossible in practical offshore and ocean engineering situations. In this paper, by means of linear mean-square estimation method, a new way to extend short-term data to long-term ones is developed. The long-term data about concerning sea areas can be constructed via a series of long-term data obtained from neighbor oceanographic stations, through relevance analysis of different data series. It is effective to cover the insufficiency of time series prediction method’s overdependence upon the length of data series, as well as the limitation of variable numbers adopted in multiple linear regression model. The storm surge data collected from three oceanographic stations located in Shandong Peninsula are taken as examples to analyze the number-selection effect of reference oceanographic stations (adjacent to the concerning sea area) and the correlation coefficients between sea sites which are selected for reference and for engineering projects construction respectively. By comparing the N-year return-period values which are calculated from observed raw data and processed data which are extended from finite data series by means of the linear mean-square estimation method, one can draw a conclusion that this method can give considerably good estimation in practical ocean engineering, in spite of different extreme value distributions about raw and processed data.     

Surface Geophysical Methods used to Verify the Karst Geological Structure in the Built-up Area: A Case Study of Specific Engineering-Geological Conditions

This article presents a research study of complex limestone karst engineering-geological conditions in the municipality Valaská near Banská Bystrica in Slovakia. The aim of the study is to demonstrate the impossibility of spatial identification of cave spaces using surface geophysical methods due to the specific engineering-geological conditions of a thick surface layer of anthropogenic fill containing highly heterogeneous anthropogenic material. Its maximum thickness is 3 m. Another specificific condition of the study area is its location in the built-up area, due to which the applicability of geophysical methods was limited. The article contains methodological recommendations to be used in analogous geological conditions with karst structures topped with anthropogenic fill, which complicates the identification of cave spaces. The recommended solution herein is the identification of the cave system using underground mapping of the karst and its projection onto the surface for which surface geophysical methods have been combined.     

逆断层地震近场地震动影响场和地表形变模拟

本文设计了一个MW7.0级地震的简单的逆断层有限移动源破裂模型,计算了断层周围64个观测点的加速度、速度和位移时程及峰值,简单讨论了近场地震动峰值、断层附近地表永久位移(或地表形变)的分布特点,并讨论了永久位移和地面运动速度大脉冲的关系。重点研究了断层附近三分量的永久位移(或地表形变)大小、方向和地震动作用特点。     

城市与工程测量EDM导线技术标准的分析

基于导线测量误差理论与电磁测距(EDM)导线的特性,分析讨论了城市与工程测量EDM导线的各项技术参数,提出了各级EDM导线技术标准的建议,通过计算验证所给出的这些技术指标是合理可靠的。     

基于全过程的本科毕业设计质量评价体系构建

对毕业设计质量评价方法进行分析,总结出考核方式单一、成绩评定不够客观及监督检查力度小是毕业设计质量评价存在的主要问题。本文从毕业设计质量考核过程化、毕业设计成果呈现方式多样化和毕业设计质量检查制度化3方面构建教师和学生共同评价的全过程质量评价体系,为全面客观评价毕业设计质量提供参考。     

桩板组合结构的技术特点及应用

介绍了桩板组合结构的组成和特点,及其在实际工程中的应用。该结构可作为滩海地区进海路路堤、人工岛护壁、保滩促淤丁坝和离岸堤主体结构。     

Sequence analysis and quantitative detection of Norwalk-like viruses in cultured oysters of China

We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future.     

应用巢式逆转录聚合酶链反应检测鱼类病毒性出血性败血症病毒(VHSV)

参照鱼类病毒性出血性败血症病毒序列,根据PCR引物设计的原则,设计巢式PCR引物,采用巢式逆转录聚合酶链反应(RT-PCR)方法,对如何快速检测鱼类病毒性出血性败血症病的方法进行了较为系统的研究,并对RT-PCR的反应条件进行了优化.结果表明,巢式RT-PCR扩增获得279bp的特异性片断,阴性对照无扩增条带.巢式RT-PCR扩增出的特异性片段经测序分析,结果证实与报道的序列完全一致.该方法最低可检测出0.1pg的鱼类病毒性出血性败血症病毒RNA.初步建立了VHSV的巢式RT-PCR检测方法,该方法灵敏、特异,可为VHSV的检测提供一个快速、有效的手段.