Development of a real-time PCR method for Thalassiosira rotula rapid detection

Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula. The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection.     

The impact of the M2 internal tide on data-assimilative model estimates of the surface tide

We use a synthetic data experiment to assess the accuracy of ocean tides estimated from satellite altimetry data, with emphasis on the impact of the phase-locked internal tide, which has a surface expression of several centimeters near its sites of genesis. Previous tidal estimates have regarded this signal as a random measurement error; however, it is deterministic and not scale-separated from the barotropic (surface) tide around complex bathymetric features. The synthetic data experiments show that the internal tide has a negligible impact on the barotropic tidal fields inferred under these circumstances, and the barotropic dissipation (a quadratic functional of the tidal fields) is in good agreement with the energetics of the three-dimensional regional primitive equations model which is the source of the synthetic data.     

真鲷早期发育阶段行为生态学研究

真鲷Pagrosomus major Temminck et Schlegel早期发育阶段游泳行为的发育可以分为:(1)旋转式运动;(2)阶梯型运动;(3)间断式水平运动和滑翔;(4)巡游以及(5)疾游五种类型。前期仔鱼的摄食方式主要是伴有甩头动作的前冲摄食;后期仔鱼的摄食主要有两种类型:一种是身体弯成S形的“S攻击型”;另一种为不作摄食姿势的“咬食攻击型”。摄食方式随着发育水平和饵料种类的不同而异。文中对发育过程中出现的攻击行为、护食行为、互食行为、猝死现象亦进行了描述。并对游泳、摄食以及各种行为的生态意义和实际意义进行了讨论。     

Numerical study of enhanced energy dissipation near a seamount

Using a two-dimensional primitive equation model, we examine nonlinear responses of a semidiurnal tidal flow impinging on a seamount with a background Garrett-Munk-like (GM-like) internal wavefield. It is found that horizontally elongated pancake-like structures of high vertical wavenumber near-inertial current shear are created both in the near-field (the region over the slope of the seamount) and far-field (the region over the flat bottom of the ocean). An important distinction is that the high vertical wavenumber near-inertial current shear is amplified only at mid-latitudes in the far-field (owing to a parametric subharmonic instability (PSI)), whereas it is amplified both at mid-and high-latitudes (above the latitude where PSI can occur) in the near-field. In order to clarify the generating mechanism for the strong shear in the near-field, additional numerical experiments are carried out with the GM-like background internal waves removed. The experiments show that the strong shear is also created, indicating that it is not caused by the interaction between the background GM-like internal waves and the semidiurnal internal tides. One possible explanation is proposed for the amplification of high vertical wavenumber near-inertial current shear in the near-field where tide residual flow resulting from tide-topography interaction plays an important role in transferring energy from high-mode internal tides to near-inertial internal waves.     

派比安台风对上海黄浦江潮位的影响及成因探讨

2000年派比安台风产生的历史第二高潮位对上海的防汛带来严重影响。本文通过比较历史上的风暴潮，从动力机制、天文高潮与增水极值相碰头，潮波共振和水利工程的影响等四个方面分析了这次风暴潮造成的高水位，及台风余振期边缘波的影响，并用SLOSH（Sea,Lake,Overland Surge from Hurricanes)模式模拟了这次风暴潮，为防台减灾的正确决策提供了理论依据。     

应用遗传神经网格方法分析赤潮监测数据

简要介绍了遗传神经网络方法的原理 ,探讨了应用遗传神经网络方法研究辽东湾海域丹麦细柱藻 (Leptocylindrusdanicus)赤潮与其环境因子之间的关系 ,计算了各环境因子对丹麦细柱藻赤潮的贡献。结果表明 ,温度、盐度、DIN的变化对研究海域丹麦细柱藻种群密度的增长有比较重要的影响 ,DIN是营养限制因子。遗传神经网络是分析赤潮监测数据的有效方法 ,值得进一步探索     

The Huelva (Spain) Tsunami-Ready Station and Its Interaction with Storm Emma (March 2018)

Abstract

The sea level station operating since 1996 at Mazagón (Huelva, Spain) has been progressively upgraded to fit tsunami warning requirements, due to its location in one of the main regions at risk. Its radar water level sensor was complemented in 2017, with the addition of a pressure sensor. The performance of both sea level sensors and their response to sea level oscillations, at different frequencies, is assessed. Particular emphasis is put on the effect of extreme events, such as Storm Emma, when alternative methods to obtain 1-min data are tested, in contrast to the one based on arithmetic means. The overall differences are small, for the whole period of study (centered-root-mean-square-error below 1?cm, for 5-min, and hourly data; similar tidal parameters and sea level oscillations with periods between 30?s and 5?min). However, during Storm Emma, the pressure sensor presents sensibly lower readings than the radar, with the centered-root-mean-square-error rising to 80?mm on the March 2^nd 2018. A new method to compute 1-min data, based on medians, reduced this value to 10?mm for the same day.     

近40a来海南岛南岸的相对海平面变化

根据海南岛榆林验潮站１９５４～１９９２年连续３９ａ潮位观测资料，经过统一基准面校正后，运用多种统计分析方法研究，得出近４０ａ来海南岛南岸的相对海平面变化呈上升趋势，相应的平均上升速率为０．６４ｍｍ／ａ，这一数值较近百年来１～２ｍｍ／ａ的全球海平面上升速率明显偏小，反映出同期海南岛南岸的地面是微弱抬升的。     

粘土矿物去除赤潮生物的动力学研究

于１９９２年４－９月进行粘土矿的絮凝赤潮生物（微型原甲藻）的动力学研究；考察粘土种类、浓度、第二组分（ＰＡＣＳ）和ＰＨ等因素对絮凝速率的影响，建立粘土矿物絮凝赤潮生物的动力学模型，从理论上分析和讨论各种因素的影响，并提出提高絮凝速率的方法和途径。结果表明，高岭土体系絮凝速率大于蒙脱土体系，其速率方程可用双分子反应来描述。其中，速率常数随絮凝过程发生变化；粒子间的相互作用能和作用半径是控制和影响絮凝