青蛤(Cyclina sinensis) TRAF6基因克隆及其在Poly I:C胁迫下的免疫应答

对青蛤(Cyclina sinensis)转录组文库筛选并克隆得到青蛤TRAF6(Cs TRAF6)基因的c DNA序列,其开放阅读框为2337bp,编码778个氨基酸,分子量为86.59k Da。Cs TRAF6蛋白包含RING型锌指结构,两个TRAF型锌指结构,coiled-coil结构域以及MATH结构域。经生物信息学及分子系统学分析表明Cs TRAF6属于TRAF6家族的一员。利用实时荧光定量PCR方法检测了Cs TRAF6基因在青蛤各个组织以及在Poly I:C侵染下在血淋巴中的时序性表达情况。结果表明,Cs TRAF6基因在青蛤血淋巴、闭壳肌、鳃、性腺、外套膜和肝脏中普遍表达,其中在血淋巴中的表达水平最高。在Poly I:C刺激后的3h,青蛤血淋巴中Cs TRAF6的表达量迅速升高,在6h达到最大值,说明青蛤的Cs TRAF6基因参与了病毒类似物侵染下的免疫应答反应。     

A Method for Automatically Selecting Aids to Navigation Based on their Spatial Influence Domains

To address the limitations of manually selecting aids to navigation (AtNs) on charts, a method for automatically selecting AtNs based on their spatial influence domains (SIDs) is proposed. First, the associations between the spatial attributes of an AtN are analyzed. Second, an SID of the AtN is defined, and a model of the SID is constructed based on the associations between the spatial attributes. Third, the importance of the location of the AtN is weighted based on the SID model. Fourth, an algorithm to automatically select AtNs based on the maximum coverage of the SIDS of preselected AtNs is developed. Finally, several AtNs are selected automatically using the algorithm. The experimental results demonstrate that (1) the proposed method can automatically select AtNs and the results comply with the requirements; (2) the automatic selection can eliminate the human-induced errors or the inconsistent results of manual selections from different operators; and (3) the efficiency of the proposed method is higher than that of current manual methods.     

The wreck of the MV Rena: spatio-temporal analysis of ship-derived contaminants in the sediments and fauna of Astrolabe Reef

ABSTRACT

The October 2011 sinking of the container ship MV Rena on Astrolabe Reef, New Zealand, provided a rare opportunity to examine the fate of shipwreck-derived contaminants on an offshore rocky reef and food chain. Analyses of trace metals, polycyclic aromatic hydrocarbons (PAHs) and organotins indicated significant but localised contamination of Astrolabe Reef but not of nearby Mōtītī Island. Three years after the grounding, PAH concentrations were greater in sediments at Astrolabe (up to 131?mg kg^?1) than at control locations, while organotins from the ship's antifouling hull paint were found exclusively in Astrolabe Reef sediments and biota. Over 80% of Astrolabe sediment samples contained tributyltin at concentrations above guideline sediment levels (>0.07?mg kg^?1). Tributyltin and its decomposition products were also recorded in sea urchins, gastropods, lobster and fishes at concentrations up to 0.2?mg kg^?1. Wreck and cargo-derived metals, particularly copper, tin and zinc, were present in some Astrolabe sediment samples above Australia and New Zealand Environment and Conservation Council guideline concentrations. However, there appeared to be only limited transmission of metals through the food chain. Copper, tin and zinc were recorded at greater concentrations in Astrolabe sea urchins and gastropods compared to control specimens, while metal concentrations in other biota were comparable across impact and control sites. Despite over 3 years having passed since the Rena grounding, the data series does not show any upward or downward trends in contaminant concentrations on Astrolabe Reef. Consequently, there is uncertainty about the long-term implications of the Rena grounding for the ecology of Astrolabe Reef.     

Interactions between whale sharks,Rhincodon typus Smith, 1928 (Orectolobiformes,Rhincodontidae), and Brazilian fisheries: The need for effective conservation measures

This study reports three interaction events between the whale shark (Rhincodon typus) and fisheries in Brazilian waters. Two events were described as incidental captures in a gillnet fishery on the northern coast of Rio de Janeiro State, in southeastern Brazil. The last event took place at the Royal Charlotte Oceanic Bank (RCOB), in the southern region of Bahia State, where five fishermen filmed their interaction with a juvenile specimen. For one of the incidental captures, the fish was landed and consumed by locals, and for the other, the shark was released. In the RCOB, the fishermen touched the whale shark with their hands, wooden sticks and even rode on it. In addition, 74 interviews were conducted with fishermen from Bahia about sightings and possible interactions with R. typus. According to statements made by the fishermen, although not frequent, the conduct reported for the RCOB occasionally takes place in the region. Evaluating information of this nature is important to support government plans that regulate fishing activities in order to reduce incidental captures and the harassment of whale sharks. Encouraging the participation of fishermen in a collaborative monitoring program for R. typus may be a good way to better understand the threats to the species at a reduced cost, particularly for developing countries, such as Brazil.     

基于船位监控系统的拖网捕捞努力量提取方法研究

为了基于船位监控系统提取拖网捕捞努力量,通过统计航速获得3个峰值,拖网作业在第2个峰值,即1~2.1 m/s,拖网作业航向差一般在–50°~50°。利用航速、航向差阈值设定,把拖网船状态划分为慢速、作业、航行,然后提取出捕捞作业状态点,1 423艘拖网船共提取到处于捕捞状态的点318 433个,合计拖网捕捞时间15 921 h,利用反距离加权插值法生成捕捞强度分布变化趋势图。捕捞努力量在渔业资源研究中是重要的参考值之一,与传统的捕捞努力量计算方法相比,该方法具有实时、大范围、快速、分辨率高的特点,能够用于辅助渔业资源保护。     

海带磷酸甘露糖变位酶(PMM)基因的克隆与表达分析

磷酸甘露糖变位酶(PMM)是褐藻胶和岩藻聚糖合成过程中的关键酶之一。本研究利用c DNA末端快速克隆(RACE)技术,获得2条海带PMM基因(Sjpmm1,Sjpmm2)序列。其中,Sjpmm1的开放阅读框(ORF)长759 bp,其编码的Sj PMM1为卤酸脱卤酶(HAD)超家族成员,含252个氨基酸,分子量约为28.51 k Da;而Sjpmm2的ORF长1866 bp,其编码的Sj PMM2属于磷酸己糖变位酶超家族的成员,含621个氨基酸,分子量约为66.49 k Da。海带PMM的二级结构均以?-螺旋为主。进化分析表明,Sjpmm1来自于原始真核生物,而Sjpmm2来源于质体的第一次内共生作用。实时定量PCR分析发现,海带受到高温或低温胁迫时,Sjpmm1和Sjpmm2转录水平上升,以合成岩藻聚糖抵抗环境影响。此外,利用p MAL-c5X载体对Sj PMM1进行体外表达,得到高浓度的可溶性融合蛋白,为后续的Sj PMM功能分析提供基础。     

山东省三级地下水监测站网建设思考

随着国家地下水监测工程的启动,国土资源部提出了国家、省、市新的三级地下水监测站网建设要求。该文介绍了各级地下水监测站网的基本概念、主要建设内容,各级地下水监测站网建设服务对象、基本要求,细化了各级地下水监测站网建设要求,规范了建设行动。     

Outdoor recreation monitoring in coastal and marine areas – an overview of Nordic experiences and knowledge

This paper involves a collection and communication of important knowledge about and experiences with outdoor recreation monitoring in Nordic coastal and marine areas. This is a topic that so far has received little attention, especially among researchers and practitioners working with outdoor recreation monitoring in Nordic coastal and marine areas, who are in need of knowledge on the topic in order to advance monitoring activities and procedures. To remedy this situation, the purpose of this paper is to provide a knowledge base by listing and describing central literature contributions with important insight into outdoor recreation monitoring in Nordic coastal and marine areas. More specifically, this includes information about: (a) where important knowledge about outdoor recreation monitoring in Nordic coastal and marine areas can be found, (b) who the main contributors are and (c) what monitoring knowledge that has been reported so far. The paper also examines what tasks lie ahead for researchers and area managers in order to improve knowledge about outdoor recreation monitoring in Nordic coastal and marine areas. The paper is a central contribution and addition to manuals on outdoor recreation monitoring that are currently available in the Nordic countries.     

Analysis of soil nail walls performance - Case study

This study surveys the performance of temporary soil nail walls with a maximum height of 29.3 m in the Yas project, located in Tehran. Some numerical models, with various modelling approaches, were developed using finite element software and the proper modelling approaches were specified and verified.The modelling results were analysed and compared with the in situ monitored data. The results demonstrate the model’s horizontal deformations are generally greater than the in situ monitored values. To decrease the differences between models and monitored results, the effect of variations of the soil parameters had been surveyed and the limits of needed variations were specified.     

浮托安装进船过程中护舷碰撞力实测研究

浮托安装法广泛应用于大型组块海上安装。导管架平台上部组块浮托安装进船过程中,风、浪、流引起的浮托驳船横向运动造成浮托驳船与导管架桩腿的碰撞,碰撞力可能会对导管架结构造成损伤。陆丰7-2上部组块浮托安装中,为了监测碰撞力大小,设计了碰撞力海上监测系统。通过在导管架外侧四个桩腿上安装光纤光栅应变传感器对碰撞过程中导管架桩腿进行应力监测,进而计算碰撞力。对碰撞过程,载荷作用位置、方向进行简化,并对载荷大小及垂向作用位置对计算的影响进行了研究。结构分析模型简化后,测点von-Mises应力与碰撞力大小成正比,对导管架整体结构建模计算并取局部结构计算比例系数,结合应力实测数据计算出进船过程中驳船对导管架桩腿碰撞力。