南极长城站潮间带丝藻和底栖硅藻色素组成和吸收光谱的分析

本文对 1 989年 2月采集长城站前高潮区岩石上附着的丝藻 ,以及在中潮区小石块和水沼边生长的底栖硅藻 ,进行吸收光谱分析、分离。鉴定出底栖硅藻的色素种类有 6种 ,它们分别是胡萝卜素、叶绿素 a、岩藻黄质、叶绿酸、新黄素、叶绿素 c。丝藻的色素种类有 9种 ,分别为胡萝卜素、脱镁叶绿素 a、叶绿素 a、叶绿素 b、叶绿酸、脱酯基叶绿酸、新黄素、叶绿素 c和脱镁叶绿素 c。潮间带底栖硅藻和丝藻有各自特定的吸收光谱 ,它们所含色素种类也有明显差异。藻类色素对不同波长光的吸收能力是不同的 ,因此可以利用吸收光谱来鉴定藻类的种类     

The distribution and flux of particulate matter in the Bideford River Estuary,Prince Edward Island,Canada

The temporal and spatial distribution of total and organic particulate matter is investigated in the Bideford River estuary. Particulate matter is homogenously distributed in both the water column and the surface sediment, due to high rates of resuspension and lateral transport. The measured mean sedimentation rate for the estuary is 183·5 g of particulate matter m^?2 day^?1, of which more than half is due to resuspension.The surface sediment of the estuary is quantitatively the dominant reservoir of organic matter, with an average of 902·5 g of particulate organic carbon (POC) m^?2 and 119·5 g of particulate organic nitrogen (PON) m^?2. Per unit surface area, the sediment contains 450 times more POC and 400 times more PON than the water column. Terrestrial erosion contributes high levels of particulate matter, both organic and inorganic, to the estuary from the surrounding watershed. Low rates of sediment export from the estuary result in the accumulation of the terrigenous material. The allochthonous input of terrigenous organic matter masks any relationship between the indigenous plant biomass and the organic matter.In the water column, a direct correlation exists between the organic matter, i.e. POC and PON, concentration and the phytoplankton biomass as measured by the plant pigments. Resuspension is responsible for the residual organic matter in the water column unaccounted for by the phytoplankton biomass.The particulate content of the water column and the surface sediment of the estuary is compared to that of the adjacent bay. Water-borne particulate matter is exported from the estuary to the bay, so that no significant differences in concentration are noted. The estuarine sediment, however, is five to six times richer in organic and silt-clay content than the bay sediment. Since sediment flux out of the estuary is restricted, the allochthonous contribution of terrigenous particulate matter to the bay sediment is minor, and the organic content of the bay sediment is directly correlated to the autochthonous plant biomass.     

Effet de la turbidité sur la dégradation des pigments phytoplanctoniques dans l'estuaire de la GirondeEffect of turbidity on phytoplanktonic pigments degradation in the Gironde Estuary

In the Gironde Estuary, most part of phytoplanktonic material carried by the rivers is mineralised in the maximum turbidity zone (MTZ). In order to follow the degradation of the phytoplanktonic material into the MTZ, we developed an in vitro approach based on the monitoring of phytoplanktonic pigments. Algal material from two chlorophytes (Scenedesmus suspicatus Chaudat and Chlamydomonas sp.) was incubated in the dark during 28 days into water samples from the Gironde estuary MTZ, at variable suspended solid concentrations (SPM) as well as in a sterilised turbid sample. First order decay constants of chlorophylls a and b and lutein increased by a factor 3 to 5 between SPM of 0 and $\text{3 }\text{g}\text{l}{}^{\mathrm{?1}}$. The production of pheophytin a in the presence of particles and the lack of degradation in the sterilised turbid sample confirmed the effect of attached bacteria on the particles. To cite this article: E. Lemaire et al., C. R. Geoscience 334 (2002) 251–258.     

The paleolimnological analysis of sediments from high mountain lake Nižné Terianske pleso in the High Tatras (Slovakia)

Sedimentological climate proxies and a 200-year long climate record, reconstructed using a data-set of European-wide meteorological data, have been compared at the high mountain lake Niné Terianske pleso in the High Tatras, Slovakia. Diatoms, chrysophyte stomatocysts, chironomids, plant pigments and spheroidal carbonaceous particles (SCPs) were analysed as well as sediment lithostratigraphic parameters. Using a radiometric approach the sediment core was dated and a depth of 4.6 cm was found to correspond to 1852 AD. The sediment accumulation rate (0.0034 g·cm^–2·yr^–1) was one of the lowest identified in the European mountain lake project, MOLAR. Despite this slow accumulation rate a remarkably coherent lithological and stratigraphic record has been recovered. The sediments of this remote mountain site, largely free from the effects of direct human impact, have been found to display a wealth of variability over the last 200 years.The record of spheroidal carbonaceous particles, indicators of anthropogenic pollution deposition, begins around 4.5–5.0 cm in depth (1833–1857). Temporal patterns are typical of European lake sites with the concentration peaking in the late 1970's. The SCP/²¹⁰Pb inventory ratio for the site is also in good agreement with the European latitudinal pattern. A strong influence of sample age on the chrysophyte assemblage composition in the upper-most 4–6 cm indicates that the main changes in the cysts have been related to long term environmental changes, probably pH. Analysis of chironomid remains revealed a stable profundal community. Chironomids as a whole showed no correlations to temperature fluctuations in the last 200 years. Relatively abundant remains of Diamesa sp. head capsules and other taxa closely associated with stream conditions in the older layers contrast with the absence of Diamesa sp. in the recent sediments. This change is considered to be evidence for the existence of a stronger, more stable inlet supplied from permanent granular snow fields in the lake basin. The most important changes in diatom assemblages were observed at 3cm. Many species of the genus Achnanthes spp. together with Navicula schmassmannii and Orthoseira roeseana made up the greatest part of the diatom community above 3 cm, being absent or rare lower in the record. A positive correlation between diatoms and mean summer temperature was found.     

Mass Spectrometry and reverse phase HPLC techniques for the identification of degraded fossil pigments in lake sediments and their application in palaeolimnology

Accurate identification of fossil pigments is essential if they are to be used as biomarker compounds in palaeolimnological studies. In recent years High Performance Liquid Chromatography (HPLC) has greatly enhanced the efficiency with which fossil pigments can be characterised and quantified. Using HPLC, undegraded pigments are typically identified through retention times, absorbance spectra and co-chromatography with authentic reference standards. However, lake sediments may also contain degraded pigments for which there are often no standards, and which may be difficult to identify using HPLC alone. In this study, we submitted HPLC fractions of fossil pigments and pigment derivatives collected from a meromictic lake in south west Tasmania, to a combination of Mass Spectrometry (MS) techniques including Electron Impact (EI) and static Liquid Secondary Ion MS (LSIMS) to identify their molecular ion characteristics and organic chemical composition. Mass Spectrometry permitted the detection of specific mass ions which were used to verify the identity of pigments and their derivatives. These included five carotenoids, chlorophyll a and derivatives, three previously described bacteriochlorophyll c derivatives with molecular weights of 770, 784, and 802, and two undescribed derivatives of bacteriochlorophyll c with molecular weights of 766 and 788. With these improved identifications we speculate on the pathways and modes of pigment degradation in the lake and asses the value of the degraded pigments as biomarkers. The use of MS permitted the identification of a greater number of signature pigments of algal and bacterial communities thus increasing the palaeolimnological value of the sediments. These methods are best applied in fossil pigment studies where there are a large number of unknown pigments and pigment degradation products, and where there are no authentic standards for co-chromatography. Practical suggestions for pigment MS are included in the discussion.     

北极新奥尔松地区湖泊沉积物色素含量变化及环境意义

大量的古气候资料和现代器测数据表明,最近100年来北极气候经历了明显的变暖过程,这必然会对脆弱的极地湖泊生态系统产生影响。以北极新奥尔松地区的湖泊沉积柱样为研究对象,对其中4种色素(叶绿素及其衍生物CD、总胡萝卜素TC、蓝藻叶黄素Myx和颤藻黄素Osc)进行了分析,结合其他物化指标如碳酸钙含量、TOC等,从历史演化的角度重点探讨了新奥尔松地区最近100年以来湖泊初级生产力演化过程。结果表明,在相对寒冷的小冰期,气候对湖泊中藻类的生长不利,湖泊生产力下降,沉积物中色素含量出现低值,生物硅含量降低;表层5cm(对应约1890AD)沉积物中有机质含量显著升高,各色素含量均明显增加,表明小冰期后气温上升,导致湖泊藻类生长迅速,湖泊生产力大大提高,但此时生物硅仍保持在相对较低的水平,这可能与其他藻类的竞争生长有关。最近100年,沉积物中Osc/Myx比值不断降低,表明该湖泊中蓝藻含量不断上升,暗示北极地区人类活动的增强可能导致湖泊营养水平增加。     

Shallow lake trophic status linked to late Holocene climate and human impacts

Lake Mattamuskeet, North Carolina, USA is a large (162 km²) and shallow (mean depth = 1 m) coastal lake, which was significantly modified to support agricultural activities following European settlement in 1850. Paleolimnological proxies measured on a 400-cm sediment core collected from Lake Mattamuskeet reveal shifts in organic matter input and primary producer community structure in response to climatic and human impacts on the lake during the late Holocene. Stratigraphic changes in organic matter content, nutrients, metals, lignin phenols and photosynthetic pigments were used to divide the sediment core into three intervals. Interval I includes sediment deposited between A.D. 360–1584 and indicates a clear-water, sand-bottom state with low algal abundance. In addition, the lake catchment area experienced two significant fires during this interval that were recorded as charcoal layers in the core around A.D. 360 and A.D. 1435 (calibrated ¹⁴C AMS dates). Trophic structure changed with the onset of Interval II (A.D. 1584–1860) when total algal abundance increased, and the primary producer community was comprised primarily of diatoms, chrysophytes, cryptophytes and cyanobacteria. During this interval there was also an increase in terrestrial organic material input into the lake as well as a shift in plant type from woody gymnosperms to non-woody angiosperms as determined from lignin data. Sediment deposited in Lake Mattamuskeet following European settlement (Interval III, A.D. 1860-present) suggests a dramatic increase in organic-matter deposition, metals, primary-producer abundance and the onset of cyanobacterial dominance. Sedimentary evidence indicates that shallow-water primary producers can respond rapidly to climate change and human development.     

Estuarine phytoplankton dynamics and shift of limiting factors: A study in the Changjiang (Yangtze River) Estuary and adjacent area

Environmental factors in estuaries are highly variable in terms of both spatial and temporal dimensions and hence phytoplankton biomass, as well as community structure, is dynamic. Two cruises were carried out in the Changjiang (Yangtze River) Estuary and adjacent area in spring and summer. The result of CHEMTAX calculation suggests that in spring diatoms and chlorophytes contribute equally to phytoplankton biomass, while phytoplankton community structure is mainly composed of diatoms in summer. We encountered blooms in summer with chlorophyll a (CHLa) over 10 μg l⁻¹ off the Changjiang Estuary and they were mainly caused by diatoms (>90%). Based on the HPLC analysis of samples collected, phytoplankton pigments mainly concentrated beyond the front between 122.5°E and 123°E where nutrients and turbidity were best balanced. Euphotic depth (Z_eu, calculated from Secchi disk depth) to surface mixed layer depth (Z_mix) ratio (i.e. Z_eu/Z_mix) were comparable in spring (average value 1.2) and the ratio increased to 5.2 in summer. Variation of the ratio indicates an apparent shift of light and physical conditions from spring to summer. Correspondingly, CHLa was positively related to Z_eu/Z_mix ratio (r² = 0.83) in spring, indicating the light limitation over the whole investigation area. On the other hand, the relationship of CHLa and Z_eu/Z_mix ratio became unclear when Z_eu/Z_mix ratio >3 in summer. This is probably due to the combination of both light limitation before the front and nutrient limitation beyond the front. In addition, evidence was found that light condition can impact the diagnostic pigments in the Changjiang Estuary.     

枯水期钦州湾浮游植物群落结构组成与分布特征

应用浮游植物特征光合色素的分析方法,研究了2011年枯水期钦州湾浮游植物的结构组成与分布特征。结果表明:枯水期含量较高的浮游植物光合色素按含量高低依次为叶绿素a、岩藻黄素、叶绿素b、青绿素和多甲藻素,其他特征光合色素的含量很低。经CHEMTAX对光合色素转化计算,枯水期普遍检出的浮游植物类群为硅藻、青绿藻和甲藻,是枯水期浮游植物的优势类群,其生物量的平均值(±标准差)分别为(2.36±2.38)μg/L、(0.87±0.53)μg/L、(0.13±0.14)μg/L,变化范围为0.18~7.45μg/L、0.10~1.80μg/L和0.02~0.60μg/L。硅藻、青绿藻和甲藻占枯水期浮游植物生物量比例的平均值(±标准差)分别为59%±21%、30%±16%、6%±4%,占比变化范围为29%~96%、1%~53%和0.4%~14%,其他藻类所占比例很低。河口和外湾靠外海域两个区域以硅藻为优势类群,内湾及外湾近岸硅藻和青绿藻共同为优势类群。河流营养盐输入量和比例的不同决定了钦州湾河口海区浮游植物群落结构的差异,大面积贝类养殖导致了内湾至外湾近岸海区硅藻比例的降低,而外湾水温的增加引起暖水性硅藻大量增长成为优势类群,在温度进一步增加和营养盐持续输入等条件下存在会发生硅藻赤潮的风险。     

放养前后鱼塘沉积物中氮、磷、有机质、色素的变化及环境意义

通过对针塘沉积物中营养盐,有机质及色素在放养前后的测试,对比分析了在人类活动（养殖）的影响下,由于鱼塘水体快速富营养化而引起底质上述指标的变化过程,其中快速沉积环境极有利于沉积色素的保存,具有较好的环境营养状况指示意义,同时有机质,色素和营养盐之间存在着密切的相关关系,这为利用色素方法研究短时间序列环境演变,尤其是在近代人类活动影响下的环境变化提供了一定的依据。