单环刺螠硫醌氧化还原酶相互作用蛋白质的筛选

硫化物是沿海水域中常见的有害物质,对于多数生物来讲,其在微摩尔级水平便可导致生物体中毒。硫醌氧化还原酶(SQR)是一种谷胱甘肽还原酶家族的膜结合黄素蛋白,为硫化物氧化解毒的一种关键酶。本研究以体外原核表达并复性的单环刺螠SQR为材料,采用His-pulldown技术和SDS-PAGE分析,从单环刺螠体壁细胞裂解液中筛选出两个可能与SQR结合的蛋白质,分子质量分别为40 ku和60 ku。通过毛细管液相色谱-离子肼质谱分析,初步判断其为细胞色素P450(cytochrome P450)和腺苷三磷酸结合盒转运蛋白(ATP-binding cassette transporter),并初步探讨了其可能的功能。     

Variability and propagation of Labrador Sea Water in the southern subpolar North Atlantic

The variability of two modes of Labrador Sea Water (LSW) (upper and deep Labrador Sea Water) and their respective spreading in the interior North Atlantic Ocean are investigated by means of repeated ship surveys carried out along the zonal WOCE line A2/AR19 located at 43–48°N (1993–2007) and along the GOOS line at about 48–51°N (1997–2002). Hydrographic section data are complemented by temperature, salinity, and velocity time series recorded by two moorings. They have been deployed at the western flank of the Mid-Atlantic Ridge (MAR) in the Newfoundland Basin during 1996–2004. The analysis of hydrographic anomalies at various longitudes points to a gradual eastward propagation of LSW-related signals, which happens on time scales of 3–6 years from the formation region towards the MAR. Interactions of the North Atlantic Current (NAC) with the Deep Western Boundary Current (DWBC) close to Flemish Cap point to the NAC being the main distributor of the different types of LSW into the interior of the Newfoundland Basin. Comparisons between the ship data and the mooring records revealed that the mooring sites are located in a region affected by highly variable flow. The mooring time series demonstrate an elevated level of variability with eddy activity and variability associated with the NAC considerably influencing the LSW signals in this region. Hydrographic data taken from Argo profiles from the vicinity of the mooring sites turned out to mimic quite well the temporal evolution captured by the moorings. There is some indication of occasional southward flow in the LSW layer near the MAR. If this can be considered as a hint to an interior LSW-route, it is at least of minor importance in comparison to the DWBC. It acts as an important supplier for the interior North Atlantic, distributing older and recently formed LSW modes southward along the MAR.     

潜艇指挥台围壳对阻力和伴流场影响数值研究

采用Reynolds平均Navier-Stokes(RANS)方法计算潜艇三维粘性流场,分析潜艇指挥台围壳对潜艇水动力性能的影响.采用全附体SUBOFF模型验证了CFD方法,通过将螺旋桨盘面处的实效伴流场、艇体表面压力分布以及模型总阻力的模拟结果与Taylor船池的实验结果进行对比.比较结果显示CFD计算结果与实验数据具有很好的一致性,表明CFD方法可以用于潜艇指挥台围壳设计的水动力计算.通过数值计算研究指挥台围壳的高度和在艇上的分布位置对其后方的流场、螺旋桨盘面处的伴流场和阻力的影响.     

Characteristics of water exchange in the Luzon Strait during September 2006

The Luzon Strait is the only deep channel that connects the South China Sea(SCS) with the Pacific.The transport through the Luzon Strait is an important process influencing the circulation,heat and water budgets of the SCS.Early observations have suggested that water enters the SCS in winter but water inflow or outflow in summer is quite controversial.On the basis of hydrographic measurements from CTD along 120° E in the Luzon Strait during the period from September 18 to 20 in 2006,the characteristics of t...     

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非球形轴对称粒子光散射的散射矩阵元素展开系数的数值计算

扼要地介绍了非球形粒子光散射的散射矩阵元素按广义球函数展开的基本理论,以及球形粒子散射矩阵元素间的关系。在此基础上较详细地阐述了计算展开系数的方法以及在程序研制中所遇到的一些问题。最后,对非球形的Ｃｈｅｂｙｓｈｅｖ粒子光散射的散射矩阵元素的展开系数进行了计算,结果表明数值技术和计算的正确性。     

水资源开发利用引起的环境负效应——以玛纳斯河流域为例

通过对玛纳斯河流域环境负效应研究,确定了水资源开发利用过程中引起环境负效应的制约因子,引入地下水动态均衡方法进行半定量分析,明确地下水量的变化为引起环境负效应的直接因素,为定量进行水资源综合开发与利用、防止环境负效应的产生提供方法和依据。     

The connection between Labrador Sea buoyancy loss, deep western boundary current strength, and Gulf Stream path in an ocean circulation model

The sensitivity of the North Atlantic gyre circulation to high latitude buoyancy forcing is explored in a global, non-eddy resolving ocean general circulation model. Increased buoyancy forcing strengthens the deep western boundary current, the northern recirculation gyre, and the North Atlantic Current, which leads to a more realistic Gulf Stream path. High latitude density fluxes and surface water mass transformation are strongly dependent on the choice of sea ice and salinity restoring boundary conditions. Coupling the ocean model to a prognostic sea ice model results in much greater buoyancy loss in the Labrador Sea compared to simulations in which the ocean is forced by prescribed sea ice boundary conditions. A comparison of bulk flux forced hindcast simulations which differ only in their sea ice and salinity restoring forcings reveals the effects of a mixed thermohaline boundary condition transport feedback whereby small, positive temperature and salinity anomalies in subpolar regions are amplified when the gyre spins up as a result of increased buoyancy loss and convection. The primary buoyancy flux effects of the sea ice which cause the simulations to diverge are ice melt, which is less physical in the diagnostic sea ice model, and insulation of the ocean, which is less physical with the prognostic sea ice model. Increased salinity restoring ensures a more realistic net winter buoyancy loss in the Labrador Sea, but it is found that improvements in the Gulf Stream simulation can only be achieved with the excessive buoyancy loss associated with weak salinity restoring.     

东海陆坡海底块体运动地形分析方法

随着油气资源开发向深水区发展,迫切需要采用一定的方法去了解块体运动的特征以及分析块体运动对深水油气资源开发的可能性.本文阐述了东海陆坡块体运动地形分析方法以及多种数据处理技术的使用.多波束测深系统为人们提供了直观的海底地貌形态特征,地层剖面仪系统能探测海底以下沉积物性质和结构的变化.GIS、Fledermaus、Delph等软件系统可以定量分析海底微地貌形态特征.     

Impacts of systematic precipitation bias on simulations of water and energy balances in Northwest America

At high latitudes and in mountainous areas, evaluation and validation of water and energy flux simu-lations are greatly affected by systematic precipitation errors. These errors mainly come from topographic effects and undercatch of precipitation gauges. In this study, the Land Dynamics （LAD） land surface model is used to investigate impacts of systematic precipitation bias from topography and wind-blowing on water and energy flux simulation in Northwest America. The results show that topographic and wind adjustment reduced bias of streamflow simulations when compared with observed streamflow at 14 basins. These systematic biases resulted in a -50%-100% bias for runoff simulations, a -20%-20% bias for evapotranspiration, and a -40%-40% bias for sensible heat flux, subject to different locations and adjustments, when compared with the control run. Uncertain gauge adjustment leads to a 25% uncertainty for precipitation, a 20% 100% uncertainty for runoff simulation, a less-than-10% uncertainty for evapotranspiration, and a less-than-20% uncertainty for sensible heat flux.