客家文化景观基因特征 ——以湖南省炎陵县为例

以中国南方山丘区具有代表性的客家文化聚居区--炎陵县为例,采用客家文化景观基因的特征提取技术,从民居建筑、传统习俗、地方方言、宗法制度、民间信仰5个角度,提取了炎陵县客家文化景观基因的特征。研究结果表明：1）该县客家人以开放性“围堡式”的血缘宗族聚居模式,与畲、黎、瑶等少数民族通婚繁衍在山丘区的山谷地带;在“入乡随俗”谋求生存发展的环境中,某些方面已适应当地习俗,但仍保留了基本的客家精神及风俗;并以当地炎帝神农文化为主要文化参照,形成了独具炎陵县特色的客家文化。2）该县客家文化的景观基因在不同发生发展过程中有着衣、食、住、行、商等物质性方面的表达载体和地方语言、宗法制度、民间信仰、民情风俗、文化艺术等非物质性方面的表达形式。     

Cloning and expression analysis of the chloroplast fructose- 1,6-bisphosphatase gene from Pyropia haitanensis

Fructose-1,6-bisphosphatase(FBPase) is one of the key enzymes in Calvin circle and starch biosynthesis. In this study, the full-length of cpFBPase gene from Pyropia haitanensis was cloned by using rapid amplification of cDNA ends(RACE) technology. The nucleotide sequence of PhcpFBPase consists of 1 400 bp, including a 5′ untranslated region(UTR) of 92 bp, a 3′?UTR of 69 bp, and an open reading frame(ORF) of 1 236 bp, which can be translated into a 412-amino-acid putative peptides with a molecular weight of 44.3 kDa and a theoretical pI of 5.23. Multiple sequence alignment indicated that the protein belonged to the chloroplast FBPase enzyme. Phylogenetic analysis showed that the protein assembled with the cpFBPase of a thermal tolerant unicellular red micro-algae Galdieria sulphuraria. Expression patterns analyzed by qRT-PCR revealed that the expression of PhcpFBPase gene in the thallus phage was 7-fold higher than in the conchocelis phage, which suggested the different mechanisms of inorganic carbon utilization among the different life phages of P. haitanensis. And the different response modes of PhcpFBPase mRNA levels to high temperature and desiccation stress indicated that PhcpFBPase played an important role in responsing to abiotic stress.     

高应变法在判定大直径灌注桩缺陷中的应用

测试数据准确可靠的高应变法检测,能够定量分析基桩缺陷,准确判定缺陷位置。通过工程桩的低应变法和高应变法对桩身完整性判定分析的工程实例,结合钻芯法验证,总结高应变法在不同情况下对桩身完整性的评定。     

Bulk-rock Major and Trace Element Compositions of Abyssal Peridotites: Implications for Mantle Melting, Melt Extraction and Post-melting Processes Beneath Mid-Ocean Ridges

This paper presents the first comprehensive major and traceelement data for 130 abyssal peridotite samples from the Pacificand Indian ocean ridge–transform systems. The data revealimportant features about the petrogenesis of these rocks, mantlemelting and melt extraction processes beneath ocean ridges,and elemental behaviours. Although abyssal peridotites are serpentinized,and have also experienced seafloor weathering, magmatic signaturesremain well preserved in the bulk-rock compositions. The betterinverse correlation of MgO with progressively heavier rare earthelements (REE) reflects varying amounts of melt depletion. Thismelt depletion may result from recent sub-ridge mantle melting,but could also be inherited from previous melt extraction eventsfrom the fertile mantle source. Light REE (LREE) in bulk-rocksamples are more enriched, not more depleted, than in the constituentclinopyroxenes (cpx) of the same sample suites. If the cpx LREErecord sub-ridge mantle melting processes, then the bulk-rockLREE must reflect post-melting refertilization. The significantcorrelations of LREE (e.g. La, Ce, Pr, Nd) with immobile highfield strength elements (HFSE, e.g. Nb and Zr) suggest thatenrichments of both LREE and HFSE resulted from a common magmaticprocess. The refertilization takes place in the ‘cold’thermal boundary layer (TBL) beneath ridges through which theascending melts migrate and interact with the advanced residues.The refertilization apparently did not affect the cpx relicsanalyzed for trace elements. This observation suggests grain-boundaryporous melt migration in the TBL. The ascending melts may notbe thermally ‘reactive’, and thus may have affectedonly cpx rims, which, together with precipitated olivine, entrappedmelt, and the rest of the rock, were subsequently serpentinized.Very large variations in bulk-rock Zr/Hf and Nb/Ta ratios areobserved, which are unexpected. The correlation between thetwo ratios is consistent with observations on basalts that D_Zr/D_Hf< 1 and D_Nb/D_Ta < 1. Given the identical charges (5⁺ forNb and Ta; 4⁺ for Zr and Hf) and essentially the same ionicradii (R_Nb/R_Ta = 1·000 and R_Zr/R_Hf = 1·006–1·026),yet a factor of 2 mass differences (M_Zr/M_Hf = 0·511 andM_Nb/M_Ta = 0·513), it is hypothesized that mass-dependentD values, or diffusion or mass-transfer rates may be importantin causing elemental fractionations during porous melt migrationin the TBL. It is also possible that some ‘exotic’phases with highly fractionated Zr/Hf and Nb/Ta ratios may existin these rocks, thus having ‘nugget’ effects onthe bulk-rock analyses. All these hypotheses need testing byconstraining the storage and distribution of all the incompatibletrace elements in mantle peridotite. As serpentine containsup to 13 wt % H₂O, and is stable up to 7 GPa before it is transformedto dense hydrous magnesium silicate phases that are stable atpressures of 5–50 GPa, it is possible that the serpentinizedperidotites may survive, at least partly, subduction-zone dehydration,and transport large amounts of H₂O (also Ba, Rb, Cs, K, U, Sr,Pb, etc. with elevated U/Pb ratios) into the deep mantle. Thelatter may contribute to the HIMU component in the source regionsof some oceanic basalts. KEY WORDS: abyssal peridotites; serpentinization; seafloor weathering; bulk-rock major and trace element compositions; mantle melting; melt extraction; melt–residue interaction; porous flows; Nb/Ta and Zr/Hf fractionations; HIMU mantle sources     

兼顾主要元素和微量元素的部分熔融模拟计算方法及应用

本文提出了用线性约束条件下的非线性最优化方法,同时拟合部分溶融过程中的主要和微量元素;通过对新疆东天山某些花岗岩类成因的实际模拟计算,取得了良好效果;此方法解决了以往单纯考虑微量元素时难以解决的源岩矿物发生反应、残留相矿物含量难以确定等困难,赋予部分熔融程度更为实际的意义。     

High connectivity and directional gene flow in European Atlantic and Mediterranean populations of Ciona intestinalis sp. A

Understanding the factors that cause population divergence has long been of interest to marine biologists in their attempts to interpret the effect of human‐mediated vectors. Broadcast‐spawning species with limited dispersal capability are excellent candidates to measure the present‐day patterns of genetic diversity. The tunicate Ciona intestinalis (Ascidiacea) is comprised of a complex of morphologically cryptic species that form vigorous aggregates in eutrophic habitats (harbors, gulfs and lagoons) where they can compete with the epibenthic community and cause biofouling problems. This study investigated biogeographic variability and migration patterns of C. intestinalis sp. A along Northeast Atlantic and Mediterranean coasts using microsatellite markers. Data presented here on 371 specimens collected from 17 populations reveal high genetic polymorphism, but with a deficit of heterozygote deficiency. Absence of evidence for isolation by distance suggests that the genetic patterns do not reflect the geographic distribution of sampled populations. Substantial gene flow and artificial potential for dispersal boost high levels of within‐population genetic variability and prevent genetic differentiation within and between seas. A predominant eastward migration pattern was revealed by the data set, with very limited opportunity for C. intestinalis sp. A to travel westward. This directional movement indicates that other properties (e.g. habitat quality, genetic traits, mating system, life cycle) may cause adaptive divergence at a large biogeographic scale.     

Seismic performance evaluation of steel arch bridges against major earthquakes. Part 1: dynamic analysis approach

In this study the inelastic behavior of steel arch bridges subjected to strong ground motions from major earthquakes is investigated by dynamic analyses of a typical steel arch bridge using a three‐dimensional (3D) analytical model, since checking seismic performance against severe earthquakes is not usually performed when designing such kinds of bridge. The bridge considered is an upper‐deck steel arch bridge having a reinforced concrete (RC) deck, steel I‐section girders and steel arch ribs. The input ground motions are accelerograms which are modified ground motions based on the records from the 1995 Hyogoken‐Nanbu earthquake. Both the longitudinal and transverse dynamic characteristics of the bridge are studied by investigation of time‐history responses of the main parameters. It is found that seismic responses are small when subjected to the longitudinal excitation, but significantly large under the transverse ground motion due to plasticization formed in some segments such as arch rib ends and side pier bases where axial force levels are very high. Finally, a seismic performance evaluation method based on the response strain index is proposed for such steel bridge structures. Copyright © 2004 John Wiley & Sons, Ltd.     

石油烃暴露对栉孔扇贝组织Wnt4基因表达的影响

采用荧光定量PCR的方法测定了不同浓度石油烃暴露下栉孔扇贝各组织Wnt4基因相对表达量的变化,结果表明,栉孔扇贝各组织Wnt4基因表达量在不同浓度石油烃影响下变化程度具有明显差异,对照组表达量大小顺序为精巢>卵巢>鳃丝>消化盲囊;在浓度为0.1mg·L^-1石油烃影响下,除鳃丝Wnt4基因表达量有上升趋势之外,其余各组织均无显著变化(P>0.05);而在0.3mg·L^-1及以上石油烃浓度组,四种组织Wnt4基因表达量随暴露时间均出现明显上升或者被抑制的现象(P<0.05),各组织Wnt4基因被激活和被抑制的时间和程度均有不同,受石油烃暴露影响的程度为鳃丝>卵巢>精巢>消化盲囊。本研究认为0.3mg·L-1及以上浓度石油烃暴露对栉孔扇贝各组织特别是鳃丝Wnt4基因表达量的影响较大,对栉孔扇贝的生长发育影响明显;各组织Wnt4基因表达量在不同浓度石油烃的影响下呈现一定的规律性,其变化跟各组织解毒指标的变化具有一定相似性,是较好的反映栉孔扇贝在石油烃暴露下其生长发育变化程度的生物学指标。     

喜马拉雅山雪冰主要离子的时空变化特征及来源分析

对喜马拉雅山不同地区的3个雪坑和2个浅雪芯及东绒布冰川80.36 m冰芯的主要阴阳离子数据进行了分析.结果表明:主要离子浓度具有显著的季节变化特征,非季风期各主要离子浓度以高值为主,夏季风期离子浓度以低值为主;但Cl-、Na 和K 等也表现出偶然的高浓度事件.东绒布冰川雪坑和达索普浅雪芯的Na 、K 和Cl-的浓度均远高于喜马拉雅山南坡的卓奥友雪坑和Nun Kun浅雪芯的相应值,前者为后者的数倍,表明Na 、K 和Cl-的浓度受地理位置的影响显著.喜马拉雅山南坡卓奥友雪坑的NH4 浓度远高于喜马拉雅山北坡各雪坑、浅雪芯的NH4 浓度,表明喜马拉雅山对NH4 的传播起到了显著的屏障作用,但喜马拉雅山对粉尘来源离子(如Ca2 和Mg2 等)的空间贡献并不是一个有效的屏障.HYSPLIT_4模式模拟的空气轨迹图表明,喜马拉雅山地区冬春季的雪冰主要离子主要是来自南亚的塔尔沙漠以及西亚的干燥少雨的高原地区,或更遥远的北非撒哈拉沙漠,而并非通常认为的中亚和我国西北干旱、半干旱区.