Isolation and Characterization of Collagen from Squid(Ommastreoges bartrami) Skin

Collagen of squid (Ommastrephes bartrami) skin was examined in the present study. Histology showed that collagen fiber in the skin was partially cross-linked with muscle fiber. Acid-solubilized collagen (ASC) and pepsin-solubilized collagen (PSC) were extracted from the skin and characterized. The results of amino acid composition and electrophoretic patterns revealed that ASC and PSC were both type Ⅰ collagen, containing α1 and α2 chains. FTIR (fourier transform infrared spectroscopy) investigations con-firmed the existence of helical arrangements in PSC of squid skin. The denaturation temperature (Td) and shrinkage temperature (Ts)of PSC were 29.4℃ and 52.8℃, respectively.     

鱿鱼精核蛋白提取物的保健功能研究

以鱿鱼精巢组织中提取的核蛋白为主要成分制成精核蛋白提取物 ,利用功能学评价实验方法对提取物进行抗疲劳、抗氧化以及免疫调节实验。结果表明 :( 1 )该提取物能明显延长小鼠爬杆和负重游泳时间 ,减少小鼠运动时肝糖元的消耗 ,降低运动后血乳酸的水平 ;( 2 )该提取物能降低血中过氧化脂质降解产物丙二醛 ( MDA)含量、增强血中超氧化物歧化酶( SOD)活力 ;提高小鼠 NK细胞活性、巨噬细胞吞噬百分比和吞噬指数及细胞免疫水平。因而 ,该提取物有抗疲劳、抗氧化以及免疫调节作用     

发光细菌法在环渤海污水样品遗传毒性检测中的应用

建立了以经过遗传改造的发光细菌Acinetobacter sp.Rec A为受试物种的环境污染物遗传毒性快速检测方法,该方法最快可在3h内得到毒性评价结果。采用该方法评价了环渤海排污口12份污水样品的遗传毒性。环渤海12个排污口的污水样品均表现出了不同水平的遗传毒性,并呈现出一定的分布特征。其中,高毒水质集中出现在山东半岛污水样品中,中毒水质集中出现在辽东半岛地区,而低毒水质主要集中于京津冀地区。综上,在环渤海的污水样品遗传毒性检测中,这种新型发光细菌法具有快速、灵敏、简便等优点,可为以后近海环境的水质生物毒性检测提供参考依据,具有应用于近海环境水质快速监测与评价的潜力。     

A review of the assessments of the major fisheries resources in South Africa

The waters off South Africa's coastline boast a rich mix of commercially fished species. Quantitative assessments of these marine resources have developed from simple methods first applied in the 1970s, to models that encompass a wide range of methodologies. The more valuable resources have undergone regular assessments in recent decades, with frequencies closely related to the management approach employed for each fishery. Many of these assessments form the operating models used to simulation-test candidate management procedures. This paper provides a comprehensive review of the assessments of 11 of the most important fisheries resources in South Africa. Some assessments use simple biomass dynamics models, whereas others are a hybrid of age- and length-based models, each designed to model the specific characteristics of the resource and fishery concerned. Many of the assessments have been disaggregated by species/stock and/or area as related multispecies/stock/ distribution hypotheses have arisen. This paper explores the similarities and differences in the data available and the methods applied. The review indicates that, whereas the status of three of these resources cannot be estimated reliably at present, the status of six resources is considered to be reasonable to good, whereas that of abalone Haliotis midae and West Coast rock lobster Jasus lalandii remains poor.     

基于形态法的西北太平洋柔鱼种群结构研究

西北太平洋柔鱼是我国鱿钓渔业重要捕捞对象,种群结构是渔业生物学研究的基础内容.文中根据2007年7~10月40°N~45°N,151°E~158°E海域连续采集的1 342尾柔鱼样本,测定其胴长(ML)、腕长等12项形态指标,利用正态线性转化、主成分分析和判别分析等方法研究雌雄个体的种群结构.结果表明,该海域雌、雄柔鱼均存在大小2个种群.均数差异显著性表明,雌性个体2个种群在MW/ML和FW/ML存在显著差异,雄性个体在MW/ML和AL_3/ML存在显著差异,但其形态差异仍属于种内差异.主成分分析和逐步判别分析的判别准确率(雌性60.3%,雄性60.1%),说明所划分的种群在部分形态比指标上差异明显.研究认为,形态学指标可初步区分西北太平洋柔鱼种群,但需结合其他生态学指标和耳石等硬组织进一步划分.     

西北太平洋海洋净初级生产力与柔鱼资源量变动关系的研究

海洋初级生产力在海洋生态中扮演重要角色,其变化影响了海洋渔业的潜在产量。本文根据2004-2013年中国鱿钓组提供的西北太平洋柔鱼(Ommastrephes bartramii)捕捞数据和海洋遥感净初级生产力数据,研究了柔鱼冬春生西部群体资源量变动与净初级生产力的关系。结果发现,柔鱼渔场范围内净初级生产力在经度方向上呈明显的季节性变化,冬春季低,夏秋季高。捕捞月份7-11月对应的适宜净初级生产力范围分别为500~700 mg/(m2·d)(以碳计),500~800 mg/(m2·d),500~1000 mg/(m2·d),500~800 mg/(m2·d)和300~500 mg/(m2·d),最适净初级生产力分别为700 mg/(m2·d),600 mg/(m2·d),700 mg/(m2·d),600 mg/(m2·d)和400 mg/(m2·d)。7-11各月最适净初级生产力平均纬度与捕捞努力量纬度重心呈显著正相关关系(P<0.05),说明了捕捞努力量位置在渔场中不是随机分布,可能受最适净初级生产力的纬度分布的影响。柔鱼年间资源丰度与各年3月份净初级生产力以及7-11月份平均净初级生产力大小显著正相关(P<0.05)。推测每年柔鱼资源量大小可能是由3月份产卵场海域和7-11月捕捞月份渔场净初级生产力水平交互作用的结果。研究表明,异常环境条件(厄尔尼诺和拉尼娜事件)对柔鱼产卵场和渔场的净初级生产力具有显著影响,但调控机制不同。     

鱿鱼(Squid)肝脏蛋白酶解物中ACE活性抑制肽的分离纯化及活性分析

为提高鱿鱼加工下脚料的利用率,以秘鲁鱿鱼肝脏为研究对象,采用生物酶解技术和凝胶蛋白分离技术等对其进行研究。研究结果表明:鱿鱼肝脏蛋白酶解液经过超滤处理后(分子质量为20k Da),用Sephadex G-100初步分离,得到五个组分,对分离的组分进行研究,结果表明降血压活性能力最强的是组分二,测定其半抑制浓度(IC50)为1.8mg/m L。对组分二进行活性条件分析,结构表明其ACE抑制活性在温度0—100°C、p H 1—12之间基本没有变化。最后用DEAE阴离子交换柱和Sephadex LH-20对组分二继续分离纯化,得到组分六和组分七,测定其半抑制浓度(IC50)分别达到1.52 mg/m L和2.16 mg/m L。本研究可为利用鱿鱼加工下脚料开发降血压活性肽产品提供理论基础。     

Approximate analysis for relative motion of satellite formation flying in elliptical orbits

This paper studies the relative motion of satellite formation flying in arbitrary elliptical orbits with no perturbation. The trajectories of the leader and follower satellites are projected onto the celestial sphere. These two projections and celestial equator intersect each other to form a spherical triangle, in which the vertex angles and arc-distances are used to describe the relative motion equations. This method is entitled the reference orbital element approach. Here the dimensionless distance is defined as the ratio of the maximal distance between the leader and follower satellites to the semi-major axis of the leader satellite. In close formations, this dimensionless distance, as well as some vertex angles and arc-distances of this spherical triangle, and the orbital element differences are small quantities. A series of order-of-magnitude analyses about these quantities are conducted. Consequently, the relative motion equations are approximated by expansions truncated to the second order, i.e. square of the dimensionless distance. In order to study the problem of periodicity of relative motion, the semi-major axis of the follower is expanded as Taylor series around that of the leader, by regarding relative position and velocity as small quantities. Using this expansion, it is proved that the periodicity condition derived from Lawden’s equations is equivalent to the condition that the Taylor series of order one is zero. The first-order relative motion equations, simplified from the second-order ones, possess the same forms as the periodic solutions of Lawden’s equations. It is presented that the latter are further first-order approximations to the former; and moreover, compared with the latter more suitable to research spacecraft rendezvous and docking, the former are more suitable to research relative orbit configurations. The first-order relative motion equations are expanded as trigonometric series with eccentric anomaly as the angle variable. Except the terms of order one, the trigonometric series’ amplitudes are geometric series, and corresponding phases are constant both in the radial and in-track directions. When the trajectory of the in-plane relative motion is similar to an ellipse, a method to seek this ellipse is presented. The advantage of this method is shown by an example.     

Effect of flying altitude and pulse repetition frequency on laser scanner penetration rate for digital elevation model generation in a tropical forest

In tropical forests, the penetration ability of airborne laser scanning (ALS) may be limited because of highly dense vegetation cover. However, in the typical planning of ALS surveys, the ability of laser pulses to penetrate forests is not considered. Nine round-trip flight lines covering the area of a tropical forest on the northeast side of the Tsengwen Reservoir in Taiwan were designed in this study. Five flight lines flew at altitudes of 1.525, 1.830, 2.135, 2.440, and 2.745 km, and the other four had pulse repetition frequencies (PRFs) of 100, 150, 200, and 250 kHz. The laser penetration index (LPI) is a quantitative index measuring the penetration ability of the ALS and consists of the ratio of the number of laser pulses reaching the forest floor to the total number of laser pulses. The LPI was used to represent the laser penetration rate and investigate the influence of flying altitude and PRF on the LPI. The results showed that as the flying altitude decreased by 1 km, the average LPI increased by 10%, and as the PRF decreased by 50 kHz, the average LPI increased by 2%. The effect of the LPI on digital elevation models (DEMs) was confirmed by visual images obtained by DEMs at five altitudes. The DEM obtained at an altitude of 2.745 km was coarsely textured, whereas that obtained at an altitude of 1.525 km was finely textured. The in-situ height data obtained from the electronic Global Navigation Satellite System (eGNSS) were compared with the data of the ALS-generated DEMs. The results indicated that when the LPI ≥60%, the height difference between the in situ data and DEM data was not prominent. However, when the LPI <60%, the ALS-derived DEM could be higher or lower than the in-situ height; the largest difference between the two was 1.7 m. The LPI of a forest should be considered for ALS survey planning, especially when consistent DEM precision for large tropical forest areas is paramount.