Cycling of dissolved and particulate nitrogen and carbon in the Framvaren Fjord, Norway: stable isotopic variations

The reaction pathways of nitrogen and carbon in the Framvaren Fjord (Norway) were studied through stable isotope analysis (δ¹⁵N and δ¹³C) of dissolved inorganic and particulate organic matter (POM). The variations in the isotopic compositions of the various C and N pools within the water column were use to evaluate the historical deposition of material to the sediments. The high δ¹⁵N-NH₄⁺ at the O₂/H₂S interface, as a consequence of microbial uptake between 19 and 25 m, results in extremely depleted δ¹⁵N-particulate nitrogen (PN) of approximately 1‰ within the particulate maximum at approximately 19 m. The carbon isotopic distribution of dissolved inorganic carbon (DIC) and particulate organic carbon (POC) within the interface suggests that the distinct microbial flora (Chromatium sp. and Chlorobium sp.) fractionate inorganic carbon to different degrees. The extremely light δ¹³C-POC within the interface (−31‰) appears to be a result of carbon uptake by Chromatium sp. while δ¹³C-POC of −12‰ is more indicative of Chlorobium sp. Nitrogen isotopic mass balance calculations suggested that approximately 75% of the material sinking to the sediments was derived from the dense particulate maximum between 19 and 25 m. The sediment distribution of nitrogen isotopes varied from 2‰ at the surface to approximately 6‰ at 30 cm. The nitrogen isotopic variations with depth may be an indicator of the depth or position of the O₂/H₂S interface in the fjord. Low sediment δ¹⁵N indicated that the interface was within the photic zone of the water column, while more enriched values suggested that the interface was lower in the water column potentially allowing for less fractionation during biological incorporation of dissolved inorganic nitrogen. Results indicate that the dense layers of photo-autotrophic bacteria in the upper water column impart unique carbon and nitrogen isotopic signals that help follow processes within the water column and deposition to the sediments.     

Basin-To-Basin and Year-To-Year Variation of Temperature and Salinity Characteristics in the East Sea (Sea of Japan)

Analysis of CTD data from four CREAMS expeditions carried out in summers of 1993–1996 produces distinct T-S relationships for the western and eastern Japan Basin, the Ulleung Basin and the Yamato Basin. T-S characteristics are mainly determined by salinity as it changes its horizontal pattern in three layers, which are divided by isotherms of 5°C and 1°C; upper warm water, intermediate water and deep cold water. Upper warm water is most saline in the Ulleung Basin and the Yamato Basin. Salinity of intermediate water is the highest in the eastern Japan Basin. Deep cold water has the highest salinity in the Japan Basin. T-S curves in the western Japan Basin are characterized by a salinity jump around 1.2–1.4°C in the T-S plane, which was previously found off the east coast of Korea associated with the East Sea Intermediate Water (Cho and Kim, 1994). T-S curves for the Japan Basin undergo a large year-to-year variation for water warmer than 0.6°C, which occupies upper 400 m. It is postulated that the year-to-year variation in the Japan Basin is caused by convective overturning in winter. This revised version was published online in August 2006 with corrections to the Cover Date.     

福建近海主要底层经济鱼类的种群动态

本文研究了1994年福建近海底拖网渔船主要捕捞对象－带鱼,白姑鱼,二长棘鲷,短尾大眼鲷和条尾绯鲤5种底层和近底层鱼类的种群结构,生长和死亡参数及其开发比率,与1976年比较,带鱼渔获的最大年龄由1976年7龄降至1994年的4齿,白姑鱼,二长棘鲷和短尾大眼鲷由1976年或1982年的5龄降至1994年的3龄,条尾绯鲤由1976年的5龄降至1994年的4龄,带鱼的捕捞死亡系数由1976年的0．491上升到1994年的0．956。二长棘鲷由0．340上升到1．273,短尾大眼鲷由0．505上升到0．868。条尾绯鲤由0．576上升到0．924,白姑鱼由1982年的0．235上升到1994年的0．789,开发比率均比1976年或1982年有较大提高,分别在0．495,0．706,呈现充分开发或过度利用,并讨论了种群变动原因,捕捞结构和底拖网捕捞力量调整及5种鱼类的最小可捕标准问题。     

南海北部近岸海域5～6月微型硅藻

本文对南海北部近岸的南澳、海门、湛江、北海、海口、三亚等6个系样点的水样进行微型浮游植物（＜20μm)的叶绿素a含量测定,并对其微型硅藻（＜20μm)和小型硅藻（＞20μm)作计数、鉴定。结果表明,该区微型硅藻有50多种,其数量以海口最多。为84000个／dm^3;海门和江最少（500个／dm^2),文中描述了我国首次记录的8种双眉藻（Amphora).它们是Amphora castellata,A.coputata,A.cymbelloides,A.margaritifera,A.minima,A.mueeleri,A.reichardtiana和A.subacutiuscula。     

Confirmed faecal pollution to bore well waters of Mysore city

 Water-borne infections are the most common causes of infectious diseases in developing countries, often resulting from the lack of a protected water supply, a faulty water-supply system or improper sewage disposal. A recent study of 107 samples from bore wells of Mysore city has revealed that 51 samples (47.66%) contain Nitrate greater than 45 ppm, an indication that the water would be harmful for drinking. Out of 51 samples tested for faecal pollution, using a H₂S-strip test, 37 samples turned black and these were subjected to microbial identification. Out of these 37 samples, 63 H₂S producing strains were isolated and the H₂S producing organisms are Klebsiella ozaene;Klebsiella pneumoniae;Proteus mirabilis;Proteus vulgaris;Salmonella sp. and Citrobacter freundi. The frequency of occurrence of the above-mentioned six micro-organism strains are as follows: Proteus mirabilis (19);Proteus vulgaris (14);Citrobacter freundi (13);Salmonella sp. (8);Klebsiella pneumoniae (5); and Klebsiella ozaene (4). Klebsiella and Proteus are known to cause urinary infection and Salmonella intestional infection. Received: 7 September 1998 · Accepted: 10 November 1998     

2001-2020年福建沿海赤潮灾害分级和时空分布特征研究

利用2001—2020年福建沿海赤潮事件记录资料和自然灾害风险判定方法,根据赤潮成灾面积、持续时间、危害类型、渔业直接经济损失等指标综合计算赤潮灾害指数。基于自然断点法,对赤潮灾害指数进行Ⅰ级、Ⅱ级、Ⅲ级、Ⅳ级等4个灾害级别分等定级。系统分析了福建沿海赤潮生物种类、时空分布特征和演变规律。结果表明:(1)2001—2020年福建沿海赤潮以灾害程度较轻的Ⅰ级和Ⅱ级为主,灾害程度较重的Ⅲ级和Ⅳ级仅占总次数的8.9%,但其造成的渔业直接经济损失达总损失的95.0%。(2)赤潮暴发次数和面积总体呈现下降趋势,但Ⅲ级和Ⅳ级灾害频次呈现波动特征。(3)季节尺度上表现为单波峰特征,5—6月是赤潮灾害最为严重的时段,赤潮暴发次数、面积和持续时间占总体的比例分别为73.3%、84.6%和74.9%,Ⅲ级和Ⅳ级灾害占总次数的95.2%。(4)空间尺度上,福州、宁德、厦门沿海赤潮累计次数和规模较大,但Ⅲ级和Ⅳ级赤潮灾害主要分布在泉州以北的福建沿海,泉州以南的福建海域赤潮灾害级别整体较低。(5)2001—2020年福建沿海赤潮原因种逐渐增多,硅藻占比减小、甲藻占比升高,有毒赤潮主要出现在宁德、福州、泉州海域,以米氏凯伦藻(Karenia mikimotoi)引发居多。(6)硅藻赤潮主要暴发在峡湾和海湾海域,而甲藻赤潮在峡湾、海湾和开阔的近岸海域均易暴发,近年来甲藻赤潮暴发位置呈现由福建北部向南部沿海扩张的趋势。引起这些变化的原因据推测与全球气候变化及福建近岸海域环境的变化密切相关。     

大水面日本沼虾(Macrobrachium nipponensis)资源人工增殖

挑选受精卵颜色呈褐色或接近褐色的抱卵虾,放入网箱多点布局孵化,直接放流孵出幼体是大水面日本沼虾资源增殖的有效方法.2001年,采用此法对湖北省阳新县网湖日水沼虾进行增殖,其产量从增殖前(2000年)的10000kg上升至35000kg,取得显著增殖效果,进箱孵化的抱卵数量(N,kg)可用计划生产量(Y,kg),总回捕率(R)及个体平均体重(W,g/ind)计算:N≥Y·(1000·R·W)-1.     

长江口邻近海域氮同位素的周日变化特征及其对营养盐过程的指示

长江口邻近海域的氮循环过程一直是近海富营养化研究领域的热点问题, 然而目前较少有针对短周期的调查研究。本文调查了该区域氮、磷、硅等主要营养盐及多种形态的氮稳定同位素的在潮周期周日变化特征。结果显示, NO_3~-、NH_4~+、PO_4~(3-)和SiO_3~(2-)的浓度范围分别为 14.09—55.85、0.21—2.26、0.82—1.08 和 16.80—33.85μmol/L, 而δ~(15)N-NO_3~-、δ~(18)O-NO_3~-、δ~(15)N_P和δ~(13)C_P 等的分布范围分别为 4.7‰—11.1‰、-2.0‰—7.8‰、-1.2‰—7.9‰和-22.9‰— -14.7‰。NO_3~-、PO_4~(3-)和SiO_2~(3-)均与盐度呈现明显的负相关特征, 说明三者的主要来源为长江冲淡水; 4NH(10)则随盐度升高而浓度增加, 且在底层高浓度出现时刻与高盐水团输入时刻一致, 说明外海输入是该区域铵盐的主要来源。另一方面, 氮稳定同位素(δ~(15)N-NO_2~-和δ~(15)N_P)未表现出随盐度的变化规律, 而δ~(18)3O-NO_3~-和δ~(13)C_P 则随盐度升高而增加, 说明淡水输入的同位素值低于海洋水平。通过对比中、高浓度叶绿素水体中NO_3~-、δ~(15)N-NO_3~-、δ~(18)O-NO_3~-的变化特征可以看出, 随NO_3~-浓度降低, δ~(15)N-NO_3~-值升高, 且呈现 6.2‰的分馏系数, 且氧同位素也随之增大, 指示水体中浮游植物对硝酸盐的同化吸收作用。而另一方面, 两种同位素的增加差值表现出△δ~(18)O︰△δ~(15)N1 , 说明硝酸盐在被消耗的同时还发生着补充作用。在较高盐度的水体中, 发现4NH(10)呈现出向硝酸盐的转化趋势并引起δ~(15)N-NO_3~-降低, 指示了底部明显的硝化过程, 与此前的研究结果一致。本文结果以期丰富对河口区氮循环和迁移转化的认知。     

浙江南部外海鱼类种类组成和数量分布

根据2006年5月、9月、11月和2007年2月浙江南部外海渔业资源调查结果,用渔获率作为鱼类资源分布的数量指标,对浙江南部外海的鱼类种类组成、数量分布、季节变化趋势作了定量分析。结果表明,浙江南部外海调查海域鱼类种类约有205种,其中海南鲽鲆、长臂沙鲽是2个东海区新记录种。刺鲳、竹笑鱼、发光鲷、条尾鲱鲤、尖牙鲷、短鳍红娘鱼、短鳄齿鱼、带鱼、多棘腔吻鳕、花斑蛇鲻等22种鱼类是调查海域底拖网的主要捕捞鱼类,约占鱼类总渔获量的64．58％,而其它种类的渔获量较少。不同季节的经济鱼类渔获物组成相差较大,渔获量高峰期主要出现在夏、秋季,主要捕捞刺鲳、竹笑鱼、条尾鲱鲤、短鳍红娘鱼、黄鳍马面钝等,而带鱼主要出现在春、夏季和冬季。资源数量分布较高的海区是27°30′-28°30′N海域以及26°00′-27°00′N、121°30′-123°00′E海域,而春季、秋季和冬季虽然资源密度没有夏季高,秋季和冬季调查海域的鱼类资源数量明显下降,但其数量的地理分布趋势与夏季基本相同。主要种类的空间分布特点如下：刺鲳、带鱼、海鳗、日本红娘鱼、日本方头鱼等的主要渔场分布在80-100m水深海域;黑鲮蟓、真鲷、鲐鱼、高体若鳕等的主要渔场分布在100-200m水深海域;而竹笑鱼、条尾鲱鲤、短鳍红娘鱼等则在整个调查海域都有分布,属于分布范围广的种类。     

Effects of hydrostatic pressure on microbial alteration of sinking fecal pellets

We used a new experimental device called PASS (PArticle Sinking Simulator) during MedFlux to simulate changes in in situ hydrostatic pressure that particles experience sinking from mesopelagic to bathypelagic depths. Particles, largely fecal pellets, were collected at 200 m using a settling velocity NetTrap (SV NetTrap) in Ligurian Sea in April 2006 and incubated in high-pressure bottles (HPBs) of the PASS system under both atmospheric and continuously increasing pressure conditions, simulating the pressure change experienced at a sinking rate of 200 m d⁻¹. Chemical changes over time were evaluated by measuring particulate organic carbon (POC), carbohydrates, transparent exopolymer particles (TEP), amino acids, lipids, and chloropigments, as well as dissolved organic carbon (DOC) and dissolved carbohydrates. Microbial changes were evaluated microscopically, using diamidinophenylindole (DAPI) stain for total cell counts and catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH) for phylogenetic distinctions. Concentrations (normalized to POC) of particulate chloropigments, carbohydrates and TEP decreased under both sets of incubation conditions, although less under the increasing pressure regime than under atmospheric conditions. By contrast, dissolved carbohydrates (normalized to DOC) were higher after incubation and significantly higher under atmospheric conditions, suggesting they were produced at the expense of the particulate fraction. POC-normalized particulate wax/steryl esters increased only under pressure, suggesting biochemical responses of prokaryotes to the increasing pressure regime. The prokaryotic community initially consisted of 43% Bacteria, 12% Crenarchaea and 11% Euryarchaea. After incubation, Bacteria dominated (90%) the prokaryote community in all cases, with γ-Proteobacteria comprising the greatest fraction, followed by the Cytophaga–Flavobacter cluster and α-Proteobacteria group. Using the PASS system, we obtained chemical and microbial evidence that degradation by prokaryotes associated with fecal pellets sinking through mesopelagic waters is limited by the increasing pressure they experience.