基于AFLP技术的海南三亚野生和养殖牡蛎遗传多样性研究

2008年夏季,从海南三亚分别采集26份养殖牡蛎和32份野生牡蛎样品(1份样品代表1个体),开展扩增片断长度多态性(AFLP)和数量性状分析,采用POPGENE 32软件计算多态性位点百分率、遗传多样性指数、Hardy-Weinberg遗传偏离指数、群体遗传相似度和遗传距离。基于AFLP分析获得的遗传距离和线粒体细胞色素氧化酶I亚基(CO I)基因序列,采用邻接法构建养殖和野生牡蛎的系统发育树。结果表明,选择性引物对牡蛎品种具有较好的鉴别效率;相比养殖牡蛎,野生牡蛎多样性丰富、品种间同源性较低、亲缘关系相差较远、遗传基础较宽;通过CO I基因序列分析可知,养殖牡蛎均为香港巨牡蛎Crassostrea hongkongensis,而野生牡蛎属于囊牡蛎属Saccostrea,主要为僧帽牡蛎Saccostrea cucullata。目前海南三亚牡蛎种质资源多样性相对丰富,引种还未对三亚自然环境的牡蛎资源造成明显影响。     

Genetic diversity in two Japanese flounder populations from China seas inferred using microsatellite markers and COI sequences

Japanese flounder is one of the most important commercial species in China; however, information on the genetic background of natural populations in China seas is scarce. The lack of genetic data has hampered fishery management and aquaculture development programs for this species. In the present study, we have analyzed the genetic diversity in natural populations of Japanese flounder sampled from the Yellow Sea (Qingdao population, QD) and East China Sea (Zhoushan population, ZS) using 10 polymorphic microsatellite loci and cytochrome c oxidase subunit I (COI) sequencing data. A total of 68 different alleles were observed over 10 microsatellite loci. The total number of alleles per locus ranged from 2 to 9, and the number of genotypes per locus ranged from 3 to 45. The observed heterozygosity and expected heterozygosity in QD were 0.733 and 0.779, respectively, and in ZS the heterozygosity values were 0.708 and 0.783, respectively. Significant departures from Hardy-Weinberg equilibrium were observed in 7 of the 10 microsatellite loci in each of the two populations. The COI sequencing analysis revealed 25 polymorphic sites and 15 haplotypes in the two populations. The haplotype diversity and nucleotide diversity in the QD population were 0.746±0.072 8 and 0.003 34±0.001 03 respectively, and in ZS population the genetic diversity values were 0.712±0.047 0 and 0.003 18±0.000 49, respectively. The microsatellite data (F_st =0.048 7, P<0.001) and mitochondrial DNA data (F_st =0.128, P<0.001) both revealed significant genetic differentiation between the two populations. The information on the genetic variation and differentiation in Japanese flounder obtained in this study could be used to set up suitable guidelines for the management and conservation of this species, as well as for managing artificial selection programs. In future studies, more geographically diverse stocks should be used to obtain a deeper understanding of the population structure of Japanese flounder in the China seas and adjacent regions.     

Field experiments to improve the efficacy of gargoor (fish trap) fishery in Kuwait’s waters

Fish traps were investigated to understand the effects of season, bait type, trap size, and trap soak time on catch rates, catch composition, and trap loss rates from March 2004 to September 2005, to improve the performance and management of Kuwait’s gargoor (cage style fish trap) fishery, which used to be the nation’s most important one in terms of value and landings volume. Catch rates were the highest in April/May (5-8 kg/trap haul) and again in December (7 kg/trap haul). Bait type and trap size also affected catch rates and species composition. Of the seven baits tested, the best catch rates, >5 kg/trap haul, occurred with cuttlefish (Sepia pharaonis), but wolf-herring (Chirocentrus dorab) and mullet (Liza klunzingeri) also produced good results (4-5 kg/trap haul). Within the five tested sizes, the two largest-sized traps captured more fish and larger size fish. Analysis of variance (ANOVA) showed significant differences of catch rate among traps with different baits as well as among traps of different sizes. Duncan test further revealed these differences between two specific baits and sizes. Cluster Analysis of species composition showed more differences among different baits than among different trap sizes. Longer soak times did not result in larger catch rates, but increased trap loss. About 10-day soak time resulted in trap loss 7%, while 40-day soak time could result in a loss of around 20%. Consequently, it is recommended that the gargoor be checked every 10 or fewer days. The average overall catch rate during the study period was lower than that of 1980s (4.5 vs. 5.8 kg/trap haul), indicating a possible decline of fish abundance in Kuwait’s waters. It is recommended that the number of gargoor fishing boats and gargoors from each boat should be limited to allow stock rehabilitation.     

中街山水域虾类组成及其群落多样性

根据2010~2011年中街山列岛水域4个季节的渔业资源调查资料,分析了该水域虾类资源密度和群落多样性,并探讨了底层温度、底层盐度和水深对虾类数量分布和多样性的影响.结果表明:共有虾类20种,隶属于9科18属;广温广盐种无论在种类数还是尾数密度与生物量均占主导地位;虾类生物量和尾数密度在季节分布上,以冬季最高,冬季均与春、夏和秋三季呈显著差异(p值<0.05);平面分布上,生物量与密度指数总体上呈南北高、中间低,东部高西部低的特征;生物量与平均底温显著相关(P<0.05),与平均底盐和水深相关性较差(p值>0.05);葛氏长臂虾、细巧仿对虾、中国毛虾、日本鼓虾和中华管鞭虾为四季的优势种,且均属广温广盐种;虾类群落Margalef丰富度指数为0.61~1.09,Shannon-Wiener多样性指数为0.76~1.31,Pielou均匀度指数为0.50~0.70,其中丰富度指数和多样性指数春季与冬季均存在显著差异(P<0.05),均匀度指数秋季与冬季存在显著差异(p值<0.05).     

不同改良措施对退化羊草（Leymus chinensis）草原的影响

 对1983年通过不同改良措施（播种羊草、耙地、围栏封育）处理过的退化羊草草原群落特征进行比较研究,结果表明：虽然经过23 a的恢复,土壤条件仍有差别,经过耙地处理土壤容重最小,含水量最高;三种改良措施的增产效果都很明显,播种羊草、耙地、围栏封育的群落地上生物量比围栏外分别提高了127.30%、146.64%、138.04%;播种羊草和耙地处理的根茎禾草所占比例较高,而围栏外和围栏封育处理的丛生禾草所占的比例较高;三种改良措施中,旱生植物的绝对生物量都低于围栏外;耙地处理后群落多样性最高,而围栏外均匀性指数最高。综上所述：经过耙地改良所产生的效果维持时间最长,而播种羊草和围栏封育改良的后效依然存在。     

地级以上城市土地利用结构特征及影响因素差异分析

根据2007年地级以上城市的土地和社会经济数据,采用比较法、模型法和主成分法,分析其土地利用结构特征及影响因素。结果发现:①不同规模城市的居住用地、公共设施用地、工业用地、道路广场用地和绿地的比例差距明显,而仓储用地、对外交通用地、市政公用设施用地和特殊用地的比例差异较小;②随城市规模增大,居住用地、公共设施用地、工业用地比、道路广场用地的比例符合《城市用地分类与规划建设用地标准》(GBJ137-90)(以下简称《标准》)、《城市公共设施规划规范》(GB50442-2008)(以下简称《规范》)的程度呈上升趋势;③居住用地比例超出《标准》上限城市较多,且城市规模越小,超标比例越高;④中等规模以上城市的公共设施用地比例偏低,而中小城市公共设施用地比例偏高;⑤特大城市中工业用地比例超标城市数量较多,而中小城市低于《标准》下限的较多;⑥随城市规模增大,道路广场用地比例低于《标准》下限的城市所占比例降低;⑦地级以上城市中至少有1/3城市绿地比例不及《标准》下限;⑧城市规模越大,土地利用的多样化程度越高,土地利用类型越齐全,且土地利用结构多样性指数高的城市呈集聚状,呈现"群"状特点,土地利用结构均衡度递增,但优势度趋减;⑨经济因素、产业结构、交通设施水平和城市人口是影响土地利用结构的主要因素,但对不同规模城市的影响次序不同。     

中国Holdridge生命地带及其多样性的时空变化分析

在分析目前生态地理模型及其实现方法基础上,提出基于ARC/INFO与VC++综合集成的先插值再运行生命地带模型的方法和技术路线,克服了以前模型实现过程中所存在的局限性。利用中国1962～2002年735个站点逐日温度与降水量观测数据,通过对Holdridge生命地带模型和生态多样性模型进行模拟运算后获得中国Holdridge生命地带时空分布序列图及生命地带多样性序列数值。对模拟运算,结果分析发现:生命地带类型变化随着气候条件的变化与土地覆被变化存在很好的对应关系,生命地带多样性与年均生物温度、年降水量均呈现出震荡波动的变化模式。另外对中国Holdridge生命地带的多样性、连通性及斑块形状等景观指数进行计算分析,讨论了生命地带多样性与生态环境稳定性之间的相关关系。     

Proximate composition of albacore tuna,Thunnus alalunga,from the temperate South Pacific and Tasman Sea

The proximate composition was determined of the white muscle of albacore tuna, Thunnus alalunga, caught by surface trolling along the Subtropical Convergence Zone east of the South Island of New Zealand ("Area 1"), and to the north and west of the North Island ("Area 2"). Fish from Area 1 were 70 ± 10 cm (mean ± s.d.) in fork length, and higher in oil content (4.6 ± 2.9%), than Area 2 fish which measured 55 ± 10 cm and contained 2.8 ±1.7% oil. The ash content of the white muscle of all fish sampled was 1.2 ± 0.1%, and the soluble carbohydrate content 0.36 ± 0.04%. Crude protein contents were calculated at c. 25%. Statistical relationships between moisture and oil contents of both white and red muscle were determined; they can be used to predict oil content from a known moisture content. Oil content was positively correlated with fork length, and negatively correlated with the sea surface temperature at time of capture. Oil levels in the fillet were highest in the muscle layer immediately below the skin and decreased logarithmically with depth. In fish with low white‐muscle oil content (below 5%), the corresponding red‐muscle oil content was higher; above 6% white muscle oil content the corresponding red‐muscle oil content was lower. The proximate composition of whole fish, the head, frame, skin, and viscera is presented.     

Diets of alpine galaxias (Galaxias paucispondylus Stokell) and longjawed galaxias (G. prognathus Stokell) in a South Island,New Zealand,high‐country stream

Thedietsofthealpinegalaxias(Galaxias paucispondylus Stokell, 1938) and the longjawed galaxias (G. prognathus Stokell, 1940) were studied in Deep Creek, a high‐country stream in the central South Island of New Zealand. Both species are small, slender fish with entirely freshwater life cycles. Their diets were very similar and consisted of aquatic invertebrates, dominated by the larvae of Deleatidium spp. (Ephemeroptera), Hydrobiosis spp. (Trichoptera), and Chironomidae (Diptera). Although diets partly reflected what was available in the stream, both fish selected soft‐bodied prey in preference to cased or harder shelled prey. Both species are probably nocturnal feeders.     

Timing and habitat of koaro (Galaxias brevipinnis) spawning in streams draining MtTaranaki,New Zealand

A spawning site of Galaxias brevipinnis Giinther was located in a New Zealand stream for the first time. It was at the edge of a riffle and only partially submerged. The habitat used for spawning matched that described for G. brevipinnis in Australia. Spawning was estimated to have occurred between late April and early May and the eggs hatched in late May. The species of fish spawning was identified by rearing the eggs through to identifiable juveniles and by DNA sequencing of one individual.