266nm短脉冲激光对泥鳅鱼的胚胎效应

首次使用266nm激光脉冲对泥鳅受精卵进行辐照,引起畸变。辐照分别对全卵、只对胚盘、只对卵黄三种方式进行。对实验结果的分析说明266nm激光对泥鳅受精卵的作用是光化学作用,而不是光致热效应,可能引起DNA分子结构—碱基的变化。核质关系实验证明了细胞质对细脆发育过程有控制作用。     

温度、体重和摄食水平对花尾胡椒鲷幼鱼粪便比能值及吸收率的影响

鱼类没有消化的食物通过粪便而排出体外.没有消化的食物、黏液、消化道脱落细胞、代谢酶以及细菌等就组成了鱼类的粪便.排粪后所保留的食物能可以通过吸收率来估算,其计算式为:A=C-FC/C×100%,式中A(吸收率)以百分数表示;C(摄食量)等于摄入的食物质量乘以食物比能值;F(粪便排出量)等于排出的粪便质量乘以粪便比能值.吸收率在营养学上又称为消化率.粪便比能值的测定在计算吸收率时是非常必要的,但在以往对鱼类吸收率的研究[1～6]中,由于粪便收集的困难及粪便的样品量通常很少,均没有对不同条件下鱼类所排出粪便的比能值进行单独的测定.     

Microbial Diversity in Nankai Trough Sediments at a Depth of 3,843 m

Dense populations of bivalves, primarily Calyptogena sp., were observed at cold seeps of the Nankai Trough. Bacterial input to the sediment was estimated through determination of phospholipid ester-linked fatty acid (PLFA) and DNA profiles. Results indicated a bacterial biomass of 10⁹ cells (g dry wt)^-1 while individual fatty acid profiles revealed a predominance of monounsaturated fatty acids, mainly 18:1 isomers. The presence of these fatty acids can be interpreted to reflect a response to low temperature and a predominance of psychrophilic bacteria. DNA fragments encoding bacterial ribosomal RNA small-subunit sequences (16S rDNA) were amplified by the polymerase chain reaction method using DNA extracted directly from the sediment samples. From the sequencing results, at least 19 kinds of bacterial 16S rDNAs related to mostly the Proteobacteria and a few gram-positive bacteria were identified. These results suggest that the bacterial community in the Nankai Trough sediments consists of mainly bacteria belonging to the Proteobacteria , , and subdivisions. Bacteria belonging to the and subdivisions, which are known to include epibiont and sulfate reducing bacteria, respectively, were mostly detected in the sediment obtained from inside the area of the Calyptogena community, and the -Proteobacteria may function to supply reduced sulfur to bacterial endosymbionts of Calyptogena.     

黑鲷和青石斑鱼对气泡幕反应的比较

在室内水池中观察了黑鲷（Ｓｐａｒｕｓｍａｃｒｏｃｅｐｈａｌｕｓ）和青石斑鱼（Ｅｐｉｎｅｐｈｅｌｕｓａｗｏａｒａ）对孔径０．５ｍｍ、孔距５．０ｃｍ，空气压力０．２５ｋｇ／ｃｍ２固定气泡幕的反应。结果表明，气泡幕对两种海水鱼都有显著的阻拦作用，平均阻拦率分别为黑鲷７５．１％、青石斑鱼８２．４％。分析显示，气泡幕对两种鱼的阻拦率间无显著差异。在本实验中，该两种鱼对气泡幕均无明显的适应现象     

绒螯蟹种质资源研究进展

本文综述了近年来有关绒螯蟹属在形态学、遗传学和育种学方面的研究进展 ,着重介绍了同工酶、RAPD和 mt DNA技术在绒螯蟹群体遗传学中的应用 ,提出遗传学分类标准的建立是绒螯蟹属分子分类的关键所在。