MORPHOLOGICAL COMPARISON OF THE FEEDING APPARATUS OF THREE CO-OCCURRING SPECIES OF PONYFISHES (LEIOGNATHIDAE)

ImOUocrON0verevoluti0narytirne,intetspeCiliccomPctitioncanresultincharactedisplacementSthatfadiitateresourcepartitioningamongspedesffeper,l985,Wilirarnanayake,l99O).Fisharenotablyopportunisticintheirfeding,toknowWhereandhowtheybowilloftenprovidemoreinsightintotheirerelogythansbolyknowingtheactUalcomPonentSoftheirdiet(Motta,l988,HUmPhrieS,l993).ArmrdinglymorphologicalcomParisonsamongcoxiccutwspchprovidemportantinfonnationnotonlytosySteInatistsfaedwithtaxonondcprobbo,butaIsot0erelogistS…     

投喂频率对凡纳滨对虾(Litopenaeus vannamei) PI3K信号通路及糖代谢相关酶基因表达的影响

适宜的投喂频率能提高对虾的代谢和免疫能力,加快生长,实现提质增效的目标。为探讨不同投喂频率对凡纳滨对虾(Litopenaeus vannamei)糖代谢及相关信号通路的影响,以(7.6±1.0) g的凡纳滨对虾为实验材料,设置2、3、4、6次/d,共4个投喂频率处理组,每组设3个平行,实验持续14 d,实验结束时取样,测定PI3K信号通路相关因子PI3K、Akt、HIF-1α、mTOR,代谢酶基因中HK、PFK、GAPDH、PK、FBP、PEPCK,葡萄糖转运蛋白GLUT1、GLUT2 mRNA表达水平的变化。结果发现:随着投喂频率的增加, PI3K信号通路关键基因表达的水平显著上升(P<0.05),其中6次/d投喂组PI3K、Akt、HIF-1α和mTOR表达水平显著高于2次/d投喂组(P<0.05), 4次/d投喂组mTOR表达水平显著高于2次/d投喂组(P<0.05);代谢关键酶基因HK、PFK、GAPDH、PK、FBP、PEPCK的表达水平随投喂频率的增加而增加,其中6次/d投喂组糖酵解HK、PFK、GAPDH、PK酶基因表达水平显著高于2次/d投喂组(P<0.05), 3、4和6次/d投喂组糖异生酶FBP表达水平显著高于2次/d投喂组(P<0.05),葡萄糖转运蛋白GLUT2在2、3、6次/d投喂频率处理组显著高于4次/d频率投喂组(P<0.05)。得出结论认为较高的投喂频率可以通过激活凡纳滨对虾糖代谢酶基因、糖转运蛋白及PI3K信号通路的表达来提高糖代谢水平。     

基于鱼体运动特征和图像纹理特征的鱼类摄食行为识别与量化

针对水产养殖中的精准投喂问题,以大西洋鲑(Salmo salar)为研究对象,提出一种基于鱼体运动特征和图像纹理特征的鱼群摄食活动强度量化方法,进行鱼类摄食行为识别研究。利用自适应背景差分及光流法得到运动鱼体的速度、转角,并通过信息熵统计速度和转角的分布,之后通过灰度共生矩阵提取能量、熵、对比度、相关性和逆差距5个图像纹理特征值。最后,结合鱼体运动特征及图像纹理特征,对鱼类摄食行为进行识别和检测。实验结果表明,该方法的识别准确率达到了94.17%,相较于单一特征检测本研究的检测精度更高。     

Rhythms of feeding activity and food consumption of two Mediterranean burrowing fishes: Gnathophis mystax (Delaroche) and Chlopsis bicolor Rafinesque

Data on the food consumption, feeding activity and periodicity of two burrowing fish, the congrid eel Gnathophis mystax (Delaroche, 1809) and the false moray Chlopsis bicolor Rafinesque, 1810 are presented. In all, 455 specimens were collected by bottom trawl in the central Mediterranean Sea at depths ranging from 140 to 160 m during four 24‐h day–night sampling cycles. The diet of G. mystax and C. bicolor was investigated by means of stomach content analysis. Diet varied significantly with length and maturity stage. During ontogenetic development G. mystax occupied different trophic levels: diet shifted from amphipods, predominantly in the smaller congrid eels [<25 cm total length (TL)] to fish in the larger individuals (>30 cm TL). Before the transition to the icthyophagous phase, the congrid eel showed a more generalistic foraging behaviour where decapods (Solenocera membranacea, Parapenaeus longirostris, Alpheus glaber), cephalopods (Sepiola spp. and Alloteuthis media), benthic (Gobiidae, Callionymus maculatus, Arnoglossus laterna) and benthopelagic fish (Argentina sphyraena) dominated the diet. Smaller C. bicolor (<25 cm TL) fed primarily on amphipods and cephalopods; bigger specimens fed also on decapods both natantian (A. glaber, Processa canaliculata) and reptantian (Medorippe lanata, Goneplax rhomboides). Daily ration values, computed from the Eggers method, ranged from 2.48% to 2.99% wet body weight for C. bicolor and G. mystax, respectively. Diel patterns in stomach fullness and the trophic niche overlap between the two species were also discussed.     

麦垛山煤矿送料孔施工技术

为解决麦垛山巷道掘进喷浆输料困难等问题,设计施工了Ф558．8mm、深度536．27m的送料孔。介绍了该孔的钻孔施工及固井工艺。     

新型深孔立轴式回转器的研制

介绍了新型立轴式钻机的回转器与给进装置的结构特点与技术参数,以及产品在项目工地的实际应用情况。产品汲取了国内外全液压动力头的优点,具有给进行程长,维修方便等特点。     

Seasonal changes in food uptake by the sea cucumber Apostichopus japonicus in a farm pond: Evidence from C and N stable isotopes

This study investigated the seasonal changes in carbon (C) and nitrogen (N) stable isotope values of several typical food sources of Apostichopus japonicus in a farm pond, including particulate organic matter (POM), macroalgae, benthic microalgae and animals such as nematode and copepod. The stable isotope technique was used to quantify relative contributions of various sources to the food uptake by A. japonicus. The results showed that significant changes occurred in the C and N stable isotope values of sea cucumber food sources due to the seasonality of micro-or macroalgae prosperity and the fluctuation of environmental conditions. The sea cucumber A. japonicus exhibited corresponding alterations in feeding strategy in response to the changes in food conditions. Calculation with a stable isotope mixing model showed that macroalgae was the principal food source for A. japonicus throughout the 1-yr investigation, with the relative contribution averaging 28.1% - 63.2%. The relative contributions of other food sources such as copepod and nematode, POM, benthic microalgae to the total food uptake by sea cucumber averaged 22.6% - 39.1%, 6.3% - 22.2%, 2.8% - 6.5%, and 2.8% - 4.2%, respectively. Together these results indicated that the seasonal changes in food sources led to the obvious temporal differences in the relative contribution of various food sources utilized by A. japonicus. Such findings provide the basic scientific information for improving the aquaculture techniques of A. japonicus, particularly for optimizing the food environment of A. japonicus culture in farm ponds.