Formation and early development of tetraspores of Polysiphonia urceolata （Rhodomelaceae, Rhodophyta）

Polysiphonia urceolata is one type of potential commercial red seaweeds used for breeding and cultivation, because of its significant biochemical and biomedical application. However, the information of breeding and seedling incubation for cultivation is limited, especially the early development. In this study, tetrasporohyte and gametophyte of P. urceolata were taken as the study materials in Huiquan Bay, Qingdao, China. The cleaned and sterilized tetrasporophytes and gametophytes were pre-cultured in sterilized seawater, then nurtured at 18°C, 25 μmol photons m⁻² s⁻¹ in 12:12 h (light:dark) photoperiod. Continuous observation under microscope showed that the early development consists of bipolar division stage and seedling stage. In the division stage, tetraspores germinate into bipolar sporelings that further differentiate into a colorless rhizoidal portion and a lightly pigmented upright shoot. The lightly pigmented rhizoidal cell develops to a rhizoid and the larger pigmented cell transforms to an erect axis. In the seedling stage, several quasi-protuberances appear on the erect axis and form juvenile seedlings. The results demonstrate the culture of P. urceolata from tetraspores under laboratory conditions. Supported by National Key Technology Support Program, Development Program of China (No.2006AA09Z21), National Natural Science Foundation of China (No. 40618001 and N_CUHK438/06) and Shandong Agricultural Seed Stock Breeding Project     

Effect of Dietary Phosphorus on the Growth and Body Components of Juvenile Synechogobius hasta

The effect of dietary phosphorus on the growth and body components of juvenile Synechogobius hasta was determined. Different percentages of dietary phosphorus (0.63, 0.77, 0.93, 1.06, 1.22 and 1.36) were tested by feeding the fish (body weight, 15.81 g ± 0.32 g; 20 individuals each group; 3 groups each percentage) at a surplus of 5%–10% above satiation for 35 d. Dietary phosphorus did not significantly affect the specific growth rate, feed intake, feed conversion ratio and protein efficiency rate. Nitrogen ...     

Methods Comparison for Microsatellite Marker Development： Different Isolation Methods, Different Yield Efficiency

Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology, quantitative genetics and genomics. Therefore, it is extremely necessary to select several versatile, low-cost, efficient and time- and labor-saving methods to develop a large panel of microsatellite markers. In this study, we used Zhikong scallop (Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency, while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time- and cost-saving, it is difficult to obtain a large number of microsatellite markers, mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study, we recommend two methods, microsatellite-enriched library construction method and FIASCO-colony hybridization method, for large-scale microsatellite marker development. Both methods were derived from the mi-crosatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes.     

不同投喂方式对中国明对虾幼体生长发育及抗病力的研究

研究β-1,3/1,6-D-glucan及不同投喂方式对中国明对虾(Fenneropenaeus chinensis)幼体的存活、生长、发育、非特异性免疫及抗副溶血弧菌能力的影响.将β-glucan以0.25%的剂量添加到微黏合饲料中,以不同的投喂方式投喂对虾幼体,即D0(一直投喂基础饲料);D1(一直投喂含0.25%β-葡聚糖饲料);3D1-3D0(3 d投喂含0.25%β-葡聚糖的饲料-3 d投喂基础饲料);1D1-3D0(1 d投喂含0.25%β-葡聚糖的饲料-3 d投喂基础饲料),共4个处理,每个处理随机设置3个重复,对虾幼体养殖于可控温的半开放系统中,每个养殖桶中盛有50 L盐度为30～32消毒海水,养殖密度为100尾/L.实验结果表明,在孵化后14 d,投喂D1饲料组在总存活率,变态指数,体长,特定生长率方面均高于其它各组,虽然有时差异不显著.在免疫指标方面,在9和14 dph分别进行了酚氧化酶(PO)、碱性磷酸酶(ALP)、超氧化物歧化酶(SOD)、酸性磷酸酶(ACP)活力的测定,结果表明,投喂D1饲料组的4个免疫指标均高于其它3组,虽然有时差异不显著.在14 dph的攻毒实验结果表明,对照组的累积死亡率显著高于D1组(P<0.05),而1D1-3D0和3D1-3D0的累积死亡率则与其它各组均不显著.实验结果表明,间隔投喂方式并不适合于对虾幼体,在中国明对虾幼体饲料中使用β-葡聚糖时,建议添加量为0.25%,并采用一直投喂的方式.     

国外深水钢悬链线立管研究发展现状

介绍国外在新型深水立管系统--钢悬链线立管关键技术方面的研究发展现状,论述浮体一、二阶运动对钢悬链线立管疲劳寿命的影响、浮体升沉运动对钢悬链线立管触地点疲劳寿命的影响;钢悬链线立管与海底相互作用机制的实验研究及结果;钢悬链线立管涡致振动与疲劳的研究现状.并简要论述钢悬链线立管触地点问题的研究结论.     

武汉市似大地水准面GPS水准建模与软件研制

本文针对高程异常格网线性内插模型在表达光滑曲面时出现的近似误差,提出了采用基于统计学习理论的机器学习方法,结合粗差检测技术和虚拟观测值,建立高精度GPS水准似大地水准面模型。武汉市GPS水准似大地水准面几何建模实例表明了方法的可靠性与优势。单机版、WEB版、PDA版系列软件的成功研制,最大限度地实现了似大地水准面模型的普及应用。     

CBERS-02B星数据融合方法评价

CBERS—02B星(以下简称02B星)多光谱CCD数据与全色HR数据空间分辨率相差较大,给图像融合带来一定困难。在对IHS变换、主成分分析变换、Brovey 变换、GS变换等融合算法分析的基础上,利用02B星数据进行了融合试验。通过对试验结果的目视评价与定量分析发现,PC变换和GS变换方法融合图像纹理信息较清晰,光谱保真度较好。在GS变换融合中,可利用02B星CCD相机第5波段模拟GS变换的低分辨率输入,融合结果统计值优于PC变换。     

城市测绘技术进步与产品结构的发展

测绘是国民经济和社会发展的基础性保障工作,是发展高新技术又充分应用高新技术的行业,信息技术、3S技术的发展,对城市测绘行业产生深远影响,空间数据的采集技术、处理技术、管理技术和利用技术不断创新,信息采集效率、管理水平和成果质量不断提高。与此同时,城市测绘的产品日益丰富,应用日益广泛,形成包括模拟产品、数字产品、系统产品和综合服务在内的完整的产品结构体系,测绘工作的社会经济价值充分显现出来。     

基于主成分分析的ENVISAT ASAR数据在福建省漳州市香蕉园地提取中的应用(英文)

Banana is one of the main economic agrotypes in Zhangzhou, Fujian Province. The multi-temporal ENVISAT ASAR data with different polarization are used to classify the banana fields in this paper. Principal component analysis (PCA) was applied for six pairs of ASAR dual-polarization data. For its large leaves, banana has high backscatter. So the value of banana fields is high and shows very bright in the 1st component, which makes it much easier for banana fields extraction. Dual-polarization data provide more information, and the VV and VH backscatter of banana show different characters with other land covers. Based on the analysis of the radar signature of banana fields and other land covers and the 1st component, banana fields are classified using object-oriented classifier. Compared to the field survey data and ASTER data, the accuracy of banana fields in the study area is 83.5%. It shows that the principal component analysis provides the useful information in SAR images analysis and makes the extraction of banana fields easier. Supported by the Program for New Century Excellent Talents in University (NCET-05-0573), Fujian Science and Technology Project (No.2006I0018), the Science Project of the Education Department of Fujian Province(No. 2006F5022).     

主成分分析法在油荧光光谱波段选择中的应用

355nm激光器发射激光入射到海水表面,激发海表面溢油的荧光光谱,运用高光谱图像降维中应用广泛的分段主成分分析算法对油荧光光谱进行波段选择。该算法把每个分段被映射到主成分的信息量的大小作为是否被选择的标准,保证了选择波段的信息丰富;通过分段分析消除了传统主成分分析的全局性引起的波段忽略问题,获得较为满意的降维效果。