海产鱼类中异尖线虫酶消化检测技术的研究与应用

以3种海鱼(牙鲆、狭鳕、鲱鱼)为样本,针对海产品中主要的致病性寄生虫-异尖线虫,开展了酶消化检测技术的研究.根据鱼肉消化前后干物质的质量变化设计了胃蛋白酶消化效率的计算方法,并按照鱼肉:消化液＝1:10 (g/mL)的反应比例,分别确定了酶水解鱼肉的最佳条件为:初始pH值为1.1,温度为37 ℃,酶活力为8 U/mL左右;消化后所得的虫体采用多重PCR方法替代传统的形态学观察进行种属鉴定,从而初步建立了基于酶水解-多重PCR的异尖线虫的酶消化检测体系.实际样本检测表明,该技术可以较为准确、快速地用于海产鱼类中简单异尖线虫(Anisakis simplex)和伪地新线虫(Pseudoterranova decipiens)的确证性检测.     

基于网络时空核密度的交通事故多发点鉴别方法

交通事故多发点是道路交通安全管理的重要治理对象,如何利用空间统计方法对其进行高效鉴别是研究热点。以华东某地为研究区域,以2013~2015年该研究区域的道路交通事故数据为研究对象,以时空道路网络为视角,通过路网匹配和路网裁剪形成事故时空子路段,提出一种基于交通事故场景的网络时空核密度估计值作为鉴别指标,用累计频率法和零膨胀的负二项回归模型确定鉴别阈值的事故多发点鉴别方法。     

Inheritance Pattern of Microsatellite Loci and Their Use for Kinship Analysis in the Japanese Scallop Patinopecten yessoensis

The inheritance mode of seven microsatellite markers was investigated in Patinopecten yessoensis larvae from four con-trolled crosses,and the feasibility of using these markers for kinship estimation was also examined. All the seven microsatellite loci were compatible with Mendelian inheritance. Neither sex-linked barriers to transmission nor major barriers to fertilization between gametes from the parents were evident. Two of the seven loci showed the presence of null alleles in two families,suggesting the...     

Circulation and dispersion in a cancellate coast: The rivers,bays and estuaries of central Maine

The glacially carved central coast of Maine is incised by river systems with interconnecting channels, offshore-trending submarine ridges, and narrow passages between nearshore islands and headlands. The tidal range exceeds 3 m, leading to complex and vigorous circulation patterns with strong flows in narrow channels, near river mouths, and between islands. The spongiform coastal morphology allows enhanced exchange between offshore waters, estuaries and internecine bays, resulting in rapid dispersal of nutrients, larvae and contaminants throughout the region. A fine-grid numerical circulation model has been used to examine the influences of the tides, river flows and winds on the dispersion of lobster larvae and pollutants in the nearshore and riverine environment. This paper describes the model application, presents a few salient features of the circulation patterns, and examines some implications for the coastal environment. For example, under realistic tides and variable southwest summer winds, about 80% of neutral near-surface particles introduced near the offshore islands (a proxy for stage IV lobster larvae from offshore sources) remain within a few km of the islands over a two-week period. On the other hand, a persistent, periodic sea breeze can remove more than two-thirds of the particles from the domain over the same period. Tidal mixing disperses pollutants entering the upper Kennebec River to the offshore and through internecine passages in about one week.     

A common garden experiment with larval Northeast Arctic and Norwegian coastal cod cohorts in replicated mesocosms

A replicated mesocosm experiment was carried out to evaluate differential effects of feeding conditions for larval Northeast Arctic (NA) cod and Norwegian coastal (NC) cod. The two populations were (1) reared together with a 6-day older NA cohort (mixed) in high (HC) and low prey concentration (LC; 2000 and 200 prey/L initially), and (2) reared separately in HC treatments (non-mixed) to be able to evaluate both the effect of feeding conditions and possible effects of size interaction within mesocosms. The larvae were fed natural zooplankton, and the two stocks were identified in the mixed mesocosms by otolith marking. NA larvae hatched at a larger size, had higher growth rates, and survived better than NC larvae in both mixed and non-mixed mesocosms in the HC treatment. The second cohort clearly survived better in the non-mixed than in the mixed mesocosms, indicating the presence of an interaction effect before cannibalism could occur. We found a significant higher weight-at-length between NC and NA larvae (<12 mm), which was bigger than the effect difference due to feeding conditions. Furthermore, a positive relation between survival and initial growth within mesocosms was found. We suggest that lower growth at early larval stages was accompanied by lower survival, and suggest that this was further enhanced when larvae interacted with older and larger larvae.     

珠母贝人工繁育优化技术的研究

在室内水泥池分别进行了移池培育、不同种类附着器、光照强度、水流和冲洗对珠母贝附着幼虫变态和稚贝存活的研究,结果表明:附着密度为2.18个/cm~2±0.50个/cm~2时,移池培育,其变态幼虫密度为0.95个/cm~2±0.13个/cm~2,20 d稚贝存活密度0.41个/~2±0.08个/cm~2,移池培育显著提高附着幼虫变态和稚贝存活;附着板、附着绳、网片、小石块变态幼虫密度分别为0.70个/cm~2±0.08个/cm~2、1.38个/cm~2±0.15个/cm~2、0.97个/cm~2±0.12个/cm~2、1.04个/cm~2±0.28个/cm~2,稚贝存活密度分别为0.36个/cm~2±0.06个/cm~2、0.62个/cm~2±0.07个/cm~2、0.45个/cm~2±0.07个/cm~2、0.60个/cm~2±0.08个/cm~2,附着绳显著提高附着幼虫变态和稚贝存活;光照强度为300～600 lx时变态幼虫密度为0.87个/cm~2±0.07个/cm~2,20 d稚贝存活密度0.45个/cm~2±0.08个/cm~2,强光不利于幼虫的变态和稚贝存活;水流速为2.3 cm/s时变态幼虫密度为1.08个/cm~2±0.07个/cm~2,20 d稚贝存活密度0.87个/cm~2±0.07个/cm~2,冲洗的变态幼虫密度为1.64个/cm~2±0.19个/cm~2,20 d稚贝存活密度1.00个/cm~2±0.12个/cm~2,水流或冲洗显著提高附着幼虫变态和稚贝存活.     

氮钾配施对生姜产量和砂姜黑土肥力的效应

研究了淮北平原砂姜黑土地区生姜氮钾配施的增产效应和对土壤养分的影响。结果表 明:氮钾配施可以大幅度提高生姜的产量,施钾的增产幅度,柴姜为15.3%～58.4%,平均增产率 为35.8%;狮子头姜产量提高18.2%～46.9%,平均增产28.7%。增施氮钾肥可有效提高土壤有 机质、全N、有效P、速效K、缓效K、有效Ca和有效Mg含量,降低NO3-盐含量,促使土壤pH趋于 中性,对全P和碱解N含量影响较小。土壤N素和P素大量盈余,不施钾时土壤K亏损严重,施钾 处理土壤钾基本上处于盈余状态,有利于维持和提高土壤肥力。     

Evidence for the Retrograde Accretion onto Supermassive Black Holestwo

Recently, Sloan Digital Sky Survey successfully carried out the reverberation mapping of a sky area, aiming to test the R ? L relation that has been already widely used. Here, R is the responsivity-weighted radius of the broad line region, and L is the optical luminosity at 5100 Å. Two results have been obtained from the data in the first year: (1) The time lags of AGNs (Active Galactic Nuclei) with a high accretion rate are much shorter than that estimated from the R ? L relation, which confirmed the results of reverberation mapping observations made by the Lijiang 2.4 meter telescope. (2) Some AGNs with a lower accretion rate also have very short time lags. The shortening of the time lags of the AGNs with a low accretion rate is caused by the retrograde accretion of black holes. This result has verified from observations the theoretical prediction made by Wang et al. (2014). The discovery of the black holes with a retrograde accretion has important significance, it indicates that the cosmological evolution of the black holes in quasars is implemented via the inherently random accretion.     

GRS 1915+105的X射线能谱分析及黑洞自旋测量

GRS 1915+105是银河系内的低质量黑洞X射线双星,其能谱和黑洞自旋已经得到了广泛的研究.自2018年6月开始,其X射线流量下降到了低流量水平,其间偶尔会产生多波段的耀发.利用Insight-HXMT(Insight-Hard X-ray Modulation Telescope,简称为慧眼)卫星在2020年8月30日到2020年10月13日之间对GRS1915+105的观测数据,研究了其能谱特性,发现在此次爆发过程中, X射线能谱可以用一个康普顿化的多温黑体谱很好地拟合.整个爆发的硬度强度图(Hardness-Intensity Diagram, HID)一直处于软态.采用GRS1915+105的最新动力学参数M=12.4_-1.8^+2.0M⊙, i=60°±5°, D=8.6_-1.6^+2.0kpc (M、M⊙、i和D分别表示黑洞质量、太阳质量、盘倾角和距离),得到其无量纲黑洞自旋a*的一个下限a*> 0.9990,确认了GRS 1915+105是一个具有极端自旋的黑洞.考虑本地吸收体的...     

射电类星体B3 1637+436A双峰轮廓宽发射线的光变研究

通过对比间隔17yr (静止系12.5yr)的Sloan Digital Sky Survey (SDSS)、Keck LRIS (Low-Resolution Imaging Spectrograph)和Baryon Oscillation Spectroscopic Survey (BOSS)的光学光谱, 对宽线双峰射电类星体B3 1637+436A的吸积盘性质做了初步研究.该类星体的SDSS (2001年5月21日)和Keck LRIS (2003年6月28\lk 日)的连续谱没有明显差异, 而BOSS (2018月6月17日)连续谱在V波段比SDSS暗了约1.2星等.这3条光谱的Hα宽发射线均表现出明显的双峰轮廓. 和连续谱一样, 宽线双峰的发射线轮廓也被认为是来自于吸积盘.通过上述3条光谱的Hα宽线双峰的盘模型拟合发现: BOSS光谱的Hα宽线双峰可以用单个盘模型很好地拟合,谱线发射区距离中心黑洞约900--3000引力半径. 而SDSS和Keck LRIS的Hα双峰轮廓需要用两个盘模型拟合, 对应的吸积盘发射区有两个区域,其中一个发射区(``外盘'')与BOSS类似, 另一个发射区(``内盘'')位于约400--900引力半径范围内, 到中心黑洞的距离显著小于外盘. 结合连续谱的光变特征,推测内盘消失是造成SDSS/Keck LRIS光谱和BOSS光谱在连续谱和发射线轮廓上同时发生光变的主要原因.