海藻中类胡萝卜素的提取及含量测定

许多研究已经表明，类胡萝卜素是分布最广泛、最重要的一类光辅助合成色素。类胡萝卜素具有多种生物学功能：作为化学保护剂可抵抗由植物自身叶绿素的光敏氧化作用引起的有害作用，给人体补充β-胡萝卜素可有效降低心血管疾病和癌症的发生概率，高摄入富含类胡萝卜素的饮食和降低白内障发生的危险之间有重要联系(Bendich，1993)。海藻中含有丰富的类胡萝卜素，从结构类型说，通常可以分为三类：胡萝卜素、叶黄素和类胡萝卜素酸。海藻中还含有多种特有的类胡萝卜素，如褐藻黄素、甲藻黄素、硅藻黄素等。直到目前为止，海藻中类胡萝卜素的组成与生理学功能尚没有明确的结论，因而对我国丰富的海藻资源中的类胡萝卜素进行较为系统和全面的化学及生物活性研究已成为摆在我们面前的一个重要课题。本研究选取我国青岛海滨三类大型经济海藻——红、绿、褐藻中资源量丰富的代表性种类作为实验材料，较系统地测定了其中类胡萝卜素的含量，并运用经典的柱层析法对各类胡萝卜素组分进行了分离，通过薄层分析和可见光谱扫描对各分离组分进行了定性、定量分析。     

梁板分离式分层隔震结构减震原理研究

为了拓宽隔震技术的应用范围,提出了梁板分离式分层隔震结构概念,其核心思想是将框架结构或框架剪力墙结构中的所有楼层或某些楼层的全部或部分楼板通过橡胶隔震支座支承于梁上。建立了分层隔震结构的动力分析模型,利用自编程序按时程分析法和振型分解反应谱法分别计算了不同工况下分层隔震结构的地震反应,运用隔震技术的基本原理和振型分析法分析了刚度比和场地条件等因素对减震效果的影响。发现了刚度比变化过程中分层隔震结构的两个极限状态,并利用其中之一初步判断减震效果。     

Another Typical Day in this Typical Town: place as inspiration for music creation and creative expression

Geographically isolated within its own State as well as from the rest of the country, and with the highest percentage of metropolitan primacy of all Australian States, Perth is a city somewhat uncomfortable with its creative and cultural identity. The city is physically peripheral to networks in the arts and creative industries that operate at the national level. And, symbolically, many in Perth’s cultural sector feel a sense of isolation. Yet, in certain cultural sectors, such as music, Perth demonstrably punches above its weight, consistently generating high-quality cultural exports. This paper unpicks this curious juxtaposition. It examines the influence of Perth—as a geographical location and a site of creative inspiration—on the music created and disseminated by local contemporary musicians who write and perform their own indie pop/rock music. I discuss the nuanced relationship between creative clustering and place, drawing on theories regarding the relationship between music and place, the notion of the music scenes and creativity and creative practices. I explore how living in Perth, a geographically isolated city, with a highly concentrated population and associated music sector, influences creative expressions, desires and inspirations, for a select group of local indie pop/rock musicians who achieved national and international notoriety with their music in the late 1990s and 2000s. Navigating isolation but nevertheless energised by strong social connections and scene participation, Perth musicians catalysed creativity in ways that contribute to evolving debates on the links between place, creativity and music.     

扁舵鲣抗氧化肽分离纯化及结构鉴定

【目的】从扁舵鲣(Auxis thazard)的木瓜蛋白酶酶解产物中分离鉴定具有较高1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除率的抗氧化肽。【方法】用木瓜蛋白酶水解扁舵鲣,以DPPH自由基清除能力为检测指标,通过超滤、凝胶过滤层析和反向高效液相色谱分离抗氧化肽,再经过超高效液相/三重四级杆飞行时间质谱(UPLC/Xevo G2-XS QTOF)进行结构鉴定。【结果】从酶解产物中获得3种抗氧化肽,其氨基酸序列分别为β-丙氨酸-1-甲基-L-组氨酸(241 u)、Gly-Ala-Gly-Gly-Pro(357 u)和Val-Glu(246 u)。【结论】扁舵鲣的木瓜蛋白酶酶解产物含有抗氧化活性的肽类,可为其抗氧化肽的开发提供理论依据。     

Modelling of pile wave barriers by effective trenches and their screening effectiveness

The three-dimensional problem of isolation of vibration by a row of piles is studied numerically on the basis of a model replacing the row of piles by an effective trench in order to reduce the modelling complexity. The analysis is accomplished with the aid of an advanced frequency domain boundary element method, which is used for both the infilled trench and the soil medium in conjunction with a coupling procedure based on enforcement of equilibrium and compatibility at the trench–soil interface. Linear elastic or viscoelastic material behaviour is assumed for both the piles and the soil. The piles can be tubular or solid and have circular or square cross-section. The vibration source is a vertical force, harmonically varying with time, and the row of piles acts as a passive wave barrier. The effective trench model is constructed by invoking well known homogenization techniques used in the mechanics of fibre-reinforced composite materials, and its accuracy is compared against a rigorous boundary element analysis modelling each pile separately in full contact with the soil medium. On the basis of the effective trench model, the screening effectiveness of a row of piles is studied through parametric studies.     

立式储液罐橡胶基底隔震模型实验研究

针对立式储液罐地震响应问题,引伸土木结构控制思想,建立了立式储液罐橡胶基底隔震体系的试验模型,进行了地震动试验,试验结果表明,隔震体系对减轻短周期的地震影响是有效的,但对长周期的地震影响是无效的。     

含氮化合物的实验方法初步评价

对吡咯类含氮化合物的两种分离方法以及两步分离法中使用国产与进口试验的实验结果进行了对比与评价。结果表明,分离方法不同,所得吡咯类含氮化合物总体馏分的丰度也不同,一步分离法普遍低于两步法;不同分离方法对吡咯类化合物相对分布的影响较小。国产与进口试验性能不影响吡咯类含氮化合物的绝对丰度与相对分布的分析结果,两步分离法具备分析试剂国产化的条件。     

Isolation and identification of alginate-degrading bacteria and formation of alginase

I~crIONThestudiesofseaalgageneticengineeringstartedintheearly1950s.HOwever,itSdevelopmentishinderedgreatlybecauseitisdifficulttogetisolatedlivecellsandprotoplasts.TheenZymeswhicharesuitabletohigherplantsarenotsuitabletoisolatetheseaalgaecellsandproto...     

罗氏沼虾胸神经节中促进卵母细胞发育的激素的初步分离

罗氏沼虾成虾的胸神经节匀浆经Sephadex-G50柱层析所得第2个峰再经HPLC分离得到9个组分. 第1个组分和罗氏沼虾期卵巢小块一起体外培养36h可使卵母细胞的直径较对照组非常显著地增大P0.01这种增大卵母细胞直径的能力随该组分中蛋白质浓度的增加而增加. 在该组分中的激素分子量在1.3kDa左右. 结果显示罗氏沼虾胸神经节能分泌促进卵母细胞发育的激素.