环境DNA——洞穴生物研究中的新技术

中国洞穴数量多、分布广,洞穴生物资源丰富,传统生物多样性调查方法效率不高,受特殊环境影响而覆盖范围有限,一定程度上制约了中国洞穴生物多样性研究和保护工作的开展。环境DNA（eDNA）可通过从环境中提取洞穴生物的痕量DNA,利用PCR等技术对其多样性组成、生物量等进行定性和定量调查,将成为未来中国洞穴生物研究中使用的重要手段。文章对eDNA原理、在洞穴生物研究中的优势、目前取得的一些最新研究进展、主要工作流程和注意事项等进行评述,同时进一步分析中国开展相关研究所面临的问题,并展望eDNA在中国洞穴生物研究中的前景。     

北京野鸭湖湿地不同植被退化区的土壤种子库特征

2010年5月,在北京野鸭湖湿地自然保护区选择废弃鱼塘、耕作苜蓿(Medicago)地、撂荒苜蓿地、天然退化湿地和天然湿地作为实验地,其中,天然湿地作为对照。通过土壤种子库萌发实验,对实验地土壤种子库的基本特征以及与地上植被的关系进行了对比研究。结果表明,5种实验地的土壤种子库共有60种植物,隶属于23科46属。其中,萌发物种最多的是菊科(Compositae)、禾本科(Poaceae)和莎草科(Cyperaceae),分别为11种、10种和8种。废弃鱼塘、耕作苜蓿地、撂荒苜蓿地和天然退化湿地种子平均密度为6 885粒/m~2,天然湿地种子的平均密度为16 083粒/m~2,5种实验地的种子库密度差异极显著(n=300,F=4.828,p=0.001<0.05);各实验地的种子库垂直分布有一致的规律性,各实验地0～6 cm土层的种子库密度明显高于6～12 cm土层;各实验地的土壤种子库与地上植被的相似性系数都不高,土壤种子库物种组成的稳定性要高于地上植被。通过研究发现,退化湿地的土壤种子库中有大量的植物种子储藏和湿地植物的种子,这为野鸭湖退化湿地恢复提供了重要的保障,这些潜在的植物种子在野鸭湖退化湿地生态输水过程中会起到应有的恢复作用。建议野鸭湖湿地自然保护区管理部门继续加强退化湿地区域的土壤种子库保护工作,必须重视水环境的恢复、各种人为干扰的消除以及系统保护的加强。     

Peace Parks and jaguar trails: transboundary conservation in a globalizing world

An increasingly utilized strategy for expanding conservation in the developing world has been the promotion of protected areas that supersede national borders. Alternatively known as transfrontier biosphere reserves, transfrontier or transboundary conservation areas, or Peace Parks, these protected areas are aggressively advanced by conservation agencies for their purported ecological and economic benefits. This article provides a comparative assessment of two case studies to understand the various impacts of transboundary conservation. The Great Limpopo Transfrontier Park, which unites protected areas in South Africa, Mozambique and Zimbabwe, is contrasted with efforts to protect jaguars along the United States–Mexico border. We argue that while these cases are promising for the purposes of biodiversity protection, they demonstrate that transboundary conservation can minimize political context, contributes to the hegemony of international conservation agendas, and remains closely linked to economic neoliberalism and decentralization in the developing world.

Recruitment patterns of Serpula vermicularis L. (Polychaeta, Serpulidae) in Loch Creran, Scotland

This study aimed to contribute to conservation management of reefs of Serpula vermicularis by increasing understanding of the factors influencing larval settlement. The study was carried out in Loch Creran, which supports the most extensive known development of S. vermicularis reefs in the world. Settlement plates were deployed to examine the influence of season, depth, reef density, substrate type and orientation. Monthly deployment of plates revealed settlement of S. vermicularis to occur predominantly from mid-June to mid-October, peaking in late August to early September. Settlement of Pomatoceros spp. peaked much earlier, in late May to early June. Deployment of plates at different depths revealed a marked reduction in S. vermicularis settlement intensity between 6 and 12 m. As this corresponds with the deeper limit of the peripheral fringe of serpulid reefs in the loch, it is suggested that this limit is imposed by a depth-correlated settlement response, rather than reduction in available substrata. Comparisons of various substrata showed a preference by S. vermicularis larvae for a slate over a scallop substrate and no evidence of enhanced recruitment to occupied or unoccupied tubes of S. vermicularis, suggesting that gregarious attraction is unlikely to be a factor causing reef formation. Settlement onto the upper side of a horizontal scallop substrate was found to be insignificant in comparison with the underside or a vertically orientated scallop. Evidence for the role of light in controlling the depth and substrate-orientation preferences of S. vermicularis larvae is discussed. Based on the results of this study, recommendations are made regarding remediation of areas suffering reef damage.     

Biodiversity of meiofauna in the intertidal khe nhan mudflat,can gio mangrove forest,vietnam with special emphasis on free living nematodes

The ecological aspect of meiofaunal communities in Can Gio mangrove forest, Ho Chi Minh city, Vietnam has not been investigated before. The composition, distribution, density and biodiversity of meiofaunal communities were studied along an intertidal transect at the Khe Nhan mudflat. Each time, three replicate samples were collected in four stations along a transect following the water line from low tide level up to the mangrove forest edge. In total, 18 meiofaunal taxa were found with the dominant taxa belonging to Nematoda, Copepoda, Sarcomastigophora and Polychaeta. The densities of meiofauna ranged from 1156 inds/10 cm² to 2082 inds/10 cm². The increase in densities from the mangrove forest edge towards the low water line was significant. Along the mudflat transect, the biodiversity (expressed by different indices) was relatively high at different taxonomic levels but did not vary significantly along the mudflat except for taxa richness. Eighty nematode genera belonging to 24 families with Comesomatidae having the highest abundance 33.8 % were found.Theristus andNeochromadora decreased in densities from the lower water line towards the mangrove forest edge, whileParacomesoma andHopperia are typical and more abundant at the middle of the mudflat.Halalaimus increased from high on the mudflat to the low water line.     

Root structure,distribution and biomass in five multipurpose tree species of Western Himalayas

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The tree root distribution pattern and biomass of seventeen year old trees of Grewia optiva, Morus alba, Celtis australis, Bauhinia variegata and Robinia pseudoacacia were studied by excavation method. B. variegata roots penetrated to a maximum depth of 4.78 m, whereas, M. alba roots were found down to 1.48 m depth. Lateral spread was minimum in B. variegata （1.10 m）and maximum inR. pseudoacacia （7.33 m）. Maximum root biomass of 6.30 kg was found in R. pseudoacacia and minimum （2.43 kg） was found in M. alba. For four species viz.,G. optiva, M. alba, C. australis andR. pseudoacacia, 68%-87% root biomass occurred within top 0-30 cm soil depth, but forB. variegata this was only45%. The soil binding factor was maximum in G. optiva and minimum in B. variegata. Soil physico-chemical properties also showed wide variation. The study suggests thatB. variegata with a deep root system is the most suitable species for plantation under agroforestry systems. R. pseudoacacia and G. optiva with deep root systems, more lateral spread and high soil binding factor are suitable for plantation on degraded lands for soil conservation.     

Spatial correlation and ecological characteristics analysis of management area for biodiversity conservation and relevant regionalization

Formulation of different ecological zone plans according to the corresponding protection targets and the necessity of proper conservation policy is one of the measures to achieve the goal of ecological conservation in China.In order to clarify the interrelation among key ecological zone plans,this paper carried out the research on spatial relation of priority areas of biodiversity conservation and three key ecological areas（key ecological function areas,key regions of ecological service function,national nature reserves） and the research on ecological conditions,based on multi-scale ecological spatial theme information,which incorporates elements like ecologi-cal quality and type,and by the aid of spatial information analysis and GIS modeling.The results showed a contrastively fine spatial consistency with 68.8% of priority areas of biodiversity conservation overlapping with three key ecological areas.Although the envi-ronment in priority areas of biodiversity conservation were in good conditions,protection pressure is also increasing,powerful supervi-sion and protection should not be ignored.The environmental conditions in the overlapping areas,as a whole,were superior to those in the non-overlapping areas.Since two areas have different characteristics,targeted protection measures should be formulated based on this difference,which will be very important for biodiversity conservation in priority areas of biodiversity conservation.     

人工井液电阻率测井测量时间确定方法

测量时间是人工井液电阻率测井的关键参数,长期依靠施工经验来确定,尚缺少该参数的计算模型和计算方法。为解决这一问题,从影响测量时间的地层富水性、渗透率、地层水的视电阻率、示踪剂溶解速度等多种因素入手,应用质量衡算定律建立测井最佳测量时间计算模型,推导出最佳测量时间计算公式,并通过现场钻孔对比实验验证。结果表明,模型建立的最佳测量时间计算公式可以为盐化测井测量时间的选取提供可靠的依据,可为人工井液电阻率测井获取含（隔）水层水文地质参数提供技术支持,更好地指导现场水文测井工作。