民族文化旅游产品的体验化设计与创新——以彝族十月太阳历文化园为例

体验经济赋予了民族文化旅游产品新的内涵,也促使民族文化旅游产品的体验化设计成为必然.民族文化旅游产品体验化设计应遵循产品独特性、体验主题化、高度参与性、文化真实性的原则,从体验主题的确定、体验氛围营造、体验活动的策划、体验线路的安排和旅游纪念品的设计等5个方面进行.     

基于TPB拓展模型游艇旅游行为意向影响机制研究

在改进计划行为理论(TPB)模型基础上,引入人口特征和游艇文化变量,构建游艇旅游行为意向的影响机制模型,以实地调研和问卷调查作为数据来源,运用结构方程模型(SEM)实证检验影响旅游者参与游艇旅游行为意向的主要因素及其作用机制。研究表明:当前我国旅游者参与游艇旅游的意向较高;旅游者的行为态度、主观规范、感知行为控制和游艇文化是影响其选择游艇旅游活动的重要因素;旅游者的行为态度、游艇文化2个变量对游艇旅游行为意向具有显著的正向影响;主观规范和感知行为控制均通过行为态度这一中介变量间接影响游艇旅游行为意向。     

Representing environments in flux: case studies from East Africa

The dominant view in the ecology and anthropology of the 1950s saw populations harmoniously interacting in self-regulating systems; climax forests and stable societies were the ruling hypotheses. Now, however, ecology and social sciences are investigating nature and culture in flux. The flux paradigms of nature and culture describe a human–ecological relationship that is non-equilibrial, historically contingent and constantly negotiated at both material and ideological levels by unequal actors. In this paper, we examine the effect of changing ecological and cultural paradigms on interpretations of environmental change in three areas of East Africa: the North Pare Mountains, Tanzania, the Mkomazi Game Reserve, Tanzania and the Tsavo National Park, Kenya. We explore how discursive and materialist approaches can complement one another, by expanding the domains of ecological inquiry and demanding that analysts cross-check their data for unquestioned assumptions regarding stability, variability and spatial and temporal scales. Rather than testing a ruling hypothesis, we suggest that ecologists and social scientists work with multiple hypotheses, with the aim of understanding the interplay between ecological, environmental and social influences.     

Strain and culture medium optimization for production enhancement of prodiginines from marine-derived Streptomyces sp. GQQ-10

A mutant (GQQ-M6) of a Sponge-Derived streptomyces sp. GQQ-10 obtained by UV-induced mutation was used for producing prodiginines (PGs). Single factor experiments and orthogonal array design (OAD) methods were employed for medium optimization. In the single factor method, the effects of soluble starch, glucose, soybean flour, yeast extract and sodium acetate on PGs production were investigated individually. In the subsequent OAD experiments, the concentrations of these 5 key nutritional components combined with salinity were further adjusted. The mutant strain GQQ-M6 gave a 2.2-fold higher PGs production than that of the parent strain; OAD experiments offered a PGs yield of 61mg L-1, which was 10 times higher than that of the initial GQQ-10 strain under the original cultivation mode.     

转兔防御素基因小球藻培养的营养模式研究

在250mL摇瓶中研究了光自养、异养、混合营养3种营养模式对转兔防御素基因(NP-1)小球藻(Chlorella)的生长、NP-1表达量、总蛋白质含量、pH、葡萄糖消耗以及光衰减等几个方面的影响。结果表明,异养培养为转NP-1基因小球藻的最适营养模式。在此基础上,采用5,15,30L生物反应器补料分批异养培养转NP-1基因小球藻,细胞质量浓度分别达到3.67,4.87,6.39g/L。     

CHIMEHERB中的有效成分对表皮细胞培养生长的影响

为观实CHIMEHERB有效成分,对新生小白鼠和人包皮外板的表皮细胞的生长影响,本文对7个实验浓度13次实验,112组592个组织块进行了组织培养的资料分析和机理的初步探讨,结果说明CHIMEHERB对表皮细胞无任何毒副作用,能100％的促进表皮细胞的生长.其相对生长率:小白鼠组为142.73％,人包皮外板组为440.58％,实验结果与临床应用效果一致.     

海鞘血细胞培养和细菌污染控制

以海鞘(Ciona savignyi)为研究对象,考察了海鞘血细胞原代培养方法及培养中细菌污染的鉴定和控制.血细胞在培养1 h后贴壁,细胞基本以圆形细胞为主,变形细胞一般呈不规则形.变形细胞存活时间较短,而圆形细胞存活时间较长.对于培养过程中的细菌污染,通过细菌分离、培养和纯培养发现两类菌株检出率较高,均为革兰氏阴性菌.经PCR扩增16S rDNA基因序列片断,结果显示这两类菌株分别属于弧菌属(Vibrio)和施万氏菌属(Shewanella).药敏试验结果显示,弧菌属对氯霉素和环丙沙星等具有较强的敏感性;对施万氏菌属较敏感的药物依次为亚胺培南和氯霉素等.最后比较了几种抗生素组合控制血细胞培养中细菌污染的使用效果,其中氯霉素、亚胺培南加双抗的组合有较好的抑菌效果并对培养细胞的贴壁和生长没有影响.     

The effect of chlorine and chlorine dioxide on pathogenic free‐living amoebae (PFLA) in simulated natural conditions: The presence of bacteria and organic matter

Bacteria and bovine faecal matter were introduced into cultures of pathogenic free‐living amoebae, with chlorine or chlorine dioxide. The bacteria and faeces were to simulate natural conditions for a more exacting test of disinfectant demand when compared to axenic conditions. Both chlorine and chlorine dioxide were effective disinfectants under all conditions tested. Axenically and monoxenically cultured amoebae were used, the latter exerted a greater disinfectant demand.     

加强生态环境保护 促进湛江珍珠养殖业持续发展

为促进湛江珍珠养殖业的持续发展,各级政府和海域管理部门应认真贯彻执行国家有关海域管理的法律法规,加强海域环境保护的宣传教育工作,提高养殖者的环境保护意识。珍珠养殖者要创新养殖方式,拓展新的养殖空间,尊重自然规律,自觉保护海洋生态环境,以解决湛江珍珠养殖业存在的养殖海域无证使用、无度开发、生态功能丧失等问题。     

吴尚时对中国近代地理学的贡献与学术思想探讨

吴尚时（1904-1947）是中国近代地理学重要开创者之一。1928年吴尚时获金牌毕业于中山大学,次年考取公费前往法国学习地理学。他先后就读于里昂大学和格日诺布尔大学,授业于A. 阿历克斯,M.巴尔台和R.布朗夏尔等,布氏是维达尔·布拉什知名弟子。1934年吴氏学成归国,任中山大学地理系教授,1939-1945年任地理系主任。时逢日军侵华,学校不断搬迁逃避战火,华南、西南千里迁徙之途成为先生的野外考察路线,这需要非同一般的坚毅意志和对科学的献身精神。先生学术文稿泰半完成于此艰危时期,过度的劳累和极度困苦的环境严重损害了他的健康。1947年,先生去世,年仅43岁。