Isolation, distribution and evaluation of cytotoxic and antioxidant activity of cultivable actinobacteria from the Oman Sea sediments

Screening bioactive natural products from bacteria is a determinative step in the drug discovery programs. The present study aim to isolate actinobacteria from the Oman Sea sediments for determining the effects of different culture media and treatments on the yield of the isolation process, and measure the DPPH radical scavenging and Artemia cytotoxic activity of culture extracts of the actinobacterial isolates. A total of 290 actinobacterial isolates were collected from 14 sediment samples. Heat treatment(40.68%) and M_4 medium(29.31%) exhibited the maximum isolation rates of actinobacteria. Streptomyces isolates were dominantly distributed in all of the investigated stations according to 16 S rRNA gene sequencing. The distribution pattern of Streptomyces followed a depth-dependent frequency trend, whereas the members of rare genera including Micromonospora, Nocardia Actinoplanes, Nocardiopsis, Saccharopolyspora and Crossiella were distributed in deeper stations. Approximately,25% of the examined isolates could scavenge 90% of 10~(–4) mol/L DPPH solutions at 1 250 μg/mL final concentration of their ethyl acetate culture extracts. Furthermore, the most potent extracts could scavenge DPPH radicals with IC50 ranges from 356.8 to 566.4 μg/mL. Brine shrimp cytotoxicity tests showed that 38.88% of the examined culture extracts exhibited LC_(50) lower than 1 000 μg/mL against the Artemia cells. Moreover, the most potent culture extracts exhibited LC_(50) range from 335.4 to 534.4 μg/mL. Phylogenetic analysis by 16 S rRNA gene sequence revealed that the OS 005, OS 263 and OS 157 closely related to Streptomyces djakartensis, Streptomyces olivaceus and Nocardiopsis dassonvillei respectively. These results suggested the widespread distribution of the antioxidant and cytotoxic producing actinobacteria in the Oman Sea sediments, which could be considered as promising candidates for the discovery of microbial bioactive compounds.     

Confidence Evaluation of Medium-and Long-Term Earthquake Prediction by Use of the Analytical Hierarchy Process

This paper offers an approach to the relative confidence level of medium- and long-term earthquake prediction methods by use of the analytical hierarchy process (AHP). We construct the criterion judgment matrices according to the results of an expert questionnaire known as the Delphi scheme. From the matrices we can obtain a scale for the relative preference or priority weight of each factor. The quantitative weights might be applied to make synthetic prediction of earthquake risk areas in the northern part of the North China seismic region.     

辽宁省“十五”数字化地磁数据分析系统

依据台站“十五”数字化地磁工作需求,结合地磁学科组运行管理的相关技术要求,研发辽宁省“十五”数字化地磁数据分析系统.该软件采用IDL作为开发语言,Oracle 10g数据库管理数据,主要包含用户管理模块、日志管理模块和数据管理模块3部分,具备数据实时处理、快速成像、操作简便、安全稳定等特点,可以有效解决目前台站地磁数据...     

Reliability bearing capacity analysis of footings on cohesive soil slopes using RFEM

Reliability analysis of bearing capacity of a strip footing at the crest of a simple slope with cohesive soil was carried out using the random finite element method (RFEM). Analyses showed that the coefficient of variation and the spatial correlation length of soil cohesion can have a large influence on footing bearing capacity, particularly for slopes with large height to footing width ratios. The paper demonstrates cases where a footing satisfies a deterministic design factor of safety of 3 but the probability of design failure is unacceptably high. Isotropic and anisotropic spatial variability of the soil strength was also considered.     

使用价值分析优化方法在功能-技术矩阵求解中的应用

功能-技术矩阵,经众多工作者的努力研究,已渐趋成熟与实用。但如何利用功能-技术矩阵构造在广义现代设计领域中适用的科学模式,解决多学科设计方案的组成与评价问题,还存在薄弱环节。本文利用\     

城市部件数字化调查系统的研究与实现

城市部件是城市经济、社会活动的基本载体,是真正属于城市的不可移动的要素,对其进行数字化管理也是城市地理信息系统的重要组成部分.在此探讨了城市部件数字化调查系统的必要功能,详细阐述了城市部件编码方法,并且以丽水市城市部件调查为例介绍城市部件数字化调查系统的应用.     

Recent progress in estimating uncertainty in geomagnetic field modeling

Abstract

Recent work pertaining to estimating error and accuracies in geomagnetic field modeling is reviewed from a unified viewpoint and illustrated with examples. The formulation of a finite dimensional approximation to the underlying infinite dimensional problem is developed. Central to the formulation is an inner product and norm in the solution space through which a priori information can be brought to bear on the problem. Such information is crucial to estimation of the effects of higher degree fields at the Core-Mantle boundary (CMB) because the behavior of higher degree fields is masked in our measurements by the presence of the field from the Earth's crust. Contributions to the errors in predicting geophysical quantities based on the approximate model are separated into three categories: (1) the usual error from the measurement noise; (2) the error from unmodeled fields, i.e. from sources in the crust, ionosphere, etc.; and (3) the error from truncating to a finite dimensioned solution and prediction space. The combination of the first two is termed low degree error while the third is referred to as truncation error.

The error analysis problem consists of “characterizing” the difference δz = z—z, where z is some quantity depending on the magnetic field and z is the estimate of z resulting from our model. Two approaches are discussed. The method of Confidence Set Inference (CSI) seeks to find an upper bound for |z—?|. Statistical methods, i.e. Bayesian or Stochastic Estimation, seek to estimate E(δz² ), where E is the expectation value. Estimation of both the truncation error and low degree error is discussed for both approaches. Expressions are found for an upper bound for |δz| and for E(δz² ). Of particular interest is the computation of the radial field, B., at the CMB for which error estimates are made as examples of the methods. Estimated accuracies of the Gauss coefficients are given for the various methods. In general, the lowest error estimates result when the greatest amount of a priori information is available and, indeed, the estimates for truncation error are completely dependent upon the nature of the a priori information assumed. For the most conservative approach, the error in computing point values of B_r at the CMB is unbounded and one must be content with, e.g., averages over some large area. The various assumptions about a priori information are reviewed. Work is needed to extend and develop this information. In particular, information regarding the truncated fields is needed to determine if the pessimistic bounds presently available are realistic or if there is a real physical basis for lower error estimates. Characterization of crustal fields for degree greater than 50 is needed as is more rigorous characterization of the external fields.     

旋转触探数学模型及其试验分析

通过对旋转触探用双螺旋探头的受力分析,建立了螺旋探头的贯入阻力和扭矩与贯入速度和转速,以及岩土的力学性能参数间的数学模型。结合数学模型和试验数据对旋转触探技术的影响因素进行了分析,为设计探头结构和进一步理解该技术的力学机理提供了理论依据。     

不同采出程度下石油组分变化特征

利用GHM烃分析仪对水驱模拟实验过程中不同阶段的采出原油进行分析,研究其原油组成的变化规律,探索原油组成与采出程度之间的内在关系。实验表明 :原油的采出程度与采出流体PV数之间具有良好的对应关系;随着采出程度逐渐提高,原油中正构烷烃的碳数范围及主峰碳数逐渐后移,原油的轻 /重比值呈现降低趋势。实验还证明,某些在空间上呈圆柱型、具有规则支链的分子比相近碳数的直链分子更容易被驱出。该项研究对于从微观角度深入探索驱油机理、提高原油采收率具有实际意义。     

钢筋混凝土框架结构耗能分析

本文从能量的观点,利用CANNY程序对普通钢筋混凝土框架结构进行了分析.通过对3种不同框架结构在不同地震动(31条)输入下的能量反应分析,研究了滞回耗能及阻尼耗能与总输入能的关系.针对本文模型的计算结果,给出了滞回耗能与总输入能关系的表达式,并初步探讨了其影响系数.研究表明,滞回耗能及阻尼耗能与总输入能之间有良好的线性关系.