灰化法磷测定中不同灰化助剂的效果比较——以磷酸氢二钠作为磷标样

对Na 2HPO4加入不同灰化助剂经不同温度（150～550℃）灼烧后磷的回收率进行了研究。所研究的灰化助剂除文献中已深入研究过的MgSO4和Mg(No3)2外，还对MgCl2等十余种盐剂进行了实验。作者认为，在利用目前已被广泛应用的由Solбrzano和Sharp 1980年所提出的方法去分析海水中的颗粒磷和总磷或有机体中的磷时，应当用MgCl2或Mg(Ac）2作为灰化助剂而取代MgSO4。尽管Mg(NO3)2也是高效的灰化辅助剂，但其应用的危险性及更多的手工操作可能限制了其广泛应用。     

几种藻类对氮、磷的同化率与其营养成分的关系

测定了６种单细胞藻对氮，磷的同化率，光合作用速率和各藻的蛋白，氨基酸等营养成分。结果：（１）各藻的氨基酸含量与藻类对氮，磷的同化率呈线性相关；（２）藻的光合作用速率和光合作用指数分别与被藻同化的氨氮和Ｎ／Ｐ比值呈显著的相关关系。     

连续流动分析法测定污水中阴离子表面活性剂、总氮、总磷、挥发酚、氰化物、硫化物的方法及实例

快速同时测定排污口样品中阴离子表面活性剂、总氮、总磷、挥发酚、氰化物、硫化物的分析方法。采用连续流动分析法,确定最佳实验方案。经多次试验验证,呈良好线性关系;质量控制样品测定值均在保证值范围内,样品回收率在95．2％～104％之间,RSD小于2％。     

Bacterial calcification as a possible trigger for francolite precipitation under sulfidic conditions

Francolite (carbonate-fluor-apatite) is known to form in sediments where intense organic-matter decay occurs, but under oxic-to-suboxic conditions, because the alkalinity rise accompanying bacterial sulfate reduction (anoxic conditions) increases francolite solubility, hence preventing its supersaturation and precipitation. However, lagoonal, organic matter-rich, cryptalgal carbonates of Jurassic age located in the French Jura Mountains contain francolite that formed during early diagenesis, under anoxic-sulfidic and highly alkaline conditions. To explain this paradoxical situation, we propose the following “chain of reactions”: the presence of abundant biomass in the sediment would supply the “raw material” to the reactional system, i.e., the initial P budget, released through organic decay. The development of cryptalgal, bacterial mats at the sediment–water interface would have limited exchanges between the water column and the pore space, hampering pore water renewal and favoring the early onset of sulfate-reducing reactions. The onset of sulfate reduction would increase pore water alkalinity, potentially preventing francolite precipitation. In addition, the presence of sulfide ions would induce organic matter sulfurization, resulting in a relative pH rise. Alkalinity and pH rises would trigger the development of conditions leading to carbonate supersaturation, inducing bacterial-structure calcification. The sudden fall in alkalinity induced by early calcification would allow francolite precipitation, despite sulfidic conditions.     

Assessing phosphate rock depletion and phosphorus recycling options

We analyze global elemental phosphorus flows in 2009 for (1) mining to products, (2) animal and human manure flows, (3) crop harvests and animal production, (4) food production, (5) soil erosion, (6) and crop uptake. Informed by the flow assessment the potential and cost of phosphorus usage reduction and recycling measures are quantified, and fed into a constructed phosphorus supply-demand model with reserve assessment to assess the impact of these measures on phosphate rock resource availability. According to our results in 2009 globally 21.4 Mt elemental phosphorus from rock phosphate was consumed in products of which 17.6 Mt used as fertilizers, fully able to cover erosion losses and outputs in agriculture in aggregate, but insufficient from the perspective of bio-available phosphorus in soils. We find substantial scope for phosphorus use reduction, at potentially 6.9 Mt phosphorus, or 32% of 2009 phosphate rock supply. Another 6.1 Mt, or 28% can technologically be recycled from waterways and wastewater, but at a cost substantially above any foreseeable phosphate rock fertilizer price. The model results suggests phosphate rock reserves are sufficient to meet demand into the 22nd century, and can be extended well into the 23rd century with assessed use reduction and recycling measures.     

Arbuscular mycorrhizal associations in Solanum centrale (bush tomato), a perennial sub-shrub from the arid zone of Australia

Arbuscular mycorrhizal (AM) fungi convey well documented benefits to plant growth in domesticated species. We investigated AM in Solanum centrale, a desert shrub of central Australia and traditional food for Indigenous Australians. AM were observed in roots of S. centrale from wild and cultivated stands of different ages and management regimes. Greenhouse seedlings grown in sterilised sand were provided with no or minor additions of phosphorus, with or without AM fungi. Inoculated seedlings not fertilised with phosphorus exhibited moderate AM formation. Added phosphorus resulted in an absence of AM. Inoculation did not significantly affect dry weight, root length and plant height of seedlings fertilised with phosphorus but significantly increased the size of unfertilised seedlings. Inoculation significantly increased root phosphorus content, decreased root to shoot ratio and decreased root biomass at all phosphorus additions, despite the absence of observable AM. Thus it appears AM fungi in the root zone influenced certain plant characteristics, regardless of phosphorus nutrition. Overall, S. centrale benefited from the presence of AM through increased phosphorus uptake, but only when the seedlings were growing in soil with extremely low available phosphorus. The response was immediate in our experimental system and is likely to be important in the wild.     

Restoration of the eutrophic Orbetello lagoon (Tyrrhenian Sea, Italy): water quality management

The Orbetello lagoon (Tyrrhenian coast, Italy) receives treated urban and land based fishfarms wastewater. The development of severe eutrophication imposed the three main activity adoption focuses on (1) macroalgae harvesting; (2) pumping of water from the sea; (3) confining wastewater to phytotreatment ponds. The responses to these interventions were rapid and macroalgal reduction growth and seagrass return were recorded. Since 1999, a new macroalgal development was recorded. The aim of this research was to discover whether the recent macroalgal growth can be attributed to the continuing wastewater influx from the remaining persistent anthropic sources (PAS) or from the sediment nutrient release. A monitoring programme was carried out between August 1999 and July 2000 in order to measure dissolved inorganic nitrogen and phosphorus in the wastewaters entering into the lagoon and in central lagoon areas, seaweed and seagrass distribution and lagoon N, P annual budgets. The results showed higher N and P values close to PAS. The distribution of the macroalgal species confirms that the available P comes almost entirely from these remaining PAS. In conclusion, the environmental measures adopted produced a significant reduction in algal biomass development in the lagoon; the macroalgal harvesting activities produced a sediment disturbance with following oxidize conditions, which make P unavailable in the lagoon water, excepting close the PAS.     

Climate warming and the onset of salinization: Rapid changes in the limnology of two northern plains lakes

Water resources of the interior plains region of North America may be adversely affected by climate warming. The climate records of the Battleford region (west central Saskatchewan) indicate that mean annual temperatures have risen by 0.71 °C and mean annual minimum temperatures have risen by 1.03 °C from 1894 to 2007. Snowfall has also increased but total precipitation has not. Concomitant with periodic declines in precipitation, lake elevation has declined and salinity has increased in Jackfish and Murray lakes from 1938 to 2004. This long term increase in salinity is predicted to have caused an approximate 30% loss in diversity of macrobenthos. Phosphorus concentrations have also increased significantly, and Jackfish and Murray lakes would be classified as eutrophic by freshwater trophic indices. However, despite large increases in nutrients in both lakes, algal biomass has not increased and water transparency has not decreased. Although the total amount of planktonic biomass in Jackfish and Murray’s food web is similar to that of freshwater lakes, these lakes contain very low algal biomass (measured as chlorophyll a). In fact, such low algal biomass has not been previously observed in such dilute systems. The algal community in these shallow Prairie lakes appears to be very sensitive to slight changes in climate, and future climate driven increases in salinity of prairie lakes may result in large reductions in algal primary productivity.     

Soil Microbial Abundances and Enzyme Activities in Different Rhizospheres in an Integrated Vertical Flow Constructed Wetland

Rhizosphere microorganism is an important bio‐component for wastewater treatment in constructed wetlands (CWs). Microbial abundance and enzyme activities in the rhizospheres of nine plant species were investigated in an integrated vertical‐flow CW. The abundance of denitrifiers, as well as urease, acid, and alkaline phosphatase activities were positively correlated to plant root biomass. The abundance of bacteria, fungi, actinomycetes, ammonifiers, denitrifiers, and phosphorus decomposers, related to nutrient removal efficiencies in CWs, greatly varied among rhizospheres of different plant species (p < 0.05). Significant differences in rhizosphere enzyme activity among plant species were also observed (p < 0.05), with the exception of catalase activity. The principal component analysis using the data of microbial abundance and enzyme activity showed that Miscanthus floridulus, Acorus calamus, and Reineckia carnea were candidates to be used in CWs to effectively remove nitrogen and phosphorus from wastewater.