BAC end sequencing of Pacific white shrimp <Emphasis Type="Italic">Litopenaeus vannamei</Emphasis>: a glimpse into the genome of Penaeid shrimp

Little is known about the genome of Pacific white shrimp (Litopenaeus vannamei). To address this, we conducted BAC (bacterial artificial chromosome) end sequencing of L. vannamei. We selected and sequenced 7 812 BAC clones from the BAC library LvHE from the two ends of the inserts by Sanger sequencing. After trimming and quality filtering, 11 279 BAC end sequences (BESs) including 4 609 paired- ends BESs were obtained. The total length of the BESs was 4 340 753 bp, representing 0.18% of the L. vannamei haploid genome. The lengths of the BESs ranged from 100 bp to 660 bp with an average length of 385 bp. Analysis of the BESs indicated that the L. vannamei genome is AT-rich and that the primary repeats patterns were simple sequence repeats (SSRs) and low complexity sequences. Dinucleotide and hexanucleotide repeats were the most common SSR types in the BESs. The most abundant transposable element was gypsy, which may contribute to the generation of the large genome size of L. vannamei. We successfully annotated 4 519 BESs by BLAST searching, including genes involved in immunity and sex determination. Our results provide an important resource for functional gene studies, map construction and integration, and complete genome assembly for this species.     

凡纳滨对虾血淋巴、类淋巴细胞培养

以 ×L-15 培养基为基础,添加不同比例的胎牛血清和对虾肌肉提取液配制成不同的培养液,当 22×L-15培养基、胎牛血清和对虾肌肉提取液的比例分别为 60 %、20 %、20 %时,培养效果较好。培养液 pH 维持在7.0 ~ 7.2时血淋巴和类淋巴细胞生长最好,且类淋巴细胞迁出迅速。绝大部分血淋巴细胞贴壁迅速并伸展成多角样或披针形,一般存活 7 ~ 9 d,少部分呈悬浮状态的血淋巴细胞存活达 60 d 以上。培养中如未及时去除对虾血清,血淋巴细胞迅速衰老并死亡。类淋巴组织细胞 6 d可形成单层,迁出细胞主要有两种形态: 多角样和成纤维样,细胞可存活 16 d以上。     

光合细菌在对虾育苗生产中的应用

１９９５年４月２４日～５月８日，在文登高岛盐场育苗场５０ｍ３水体育苗池，进行了用几株光合细菌（ＲｈｏｄｏｐｓｅｕｄｏｍｏｎａｓＳ．Ｐ．）混合菌液作为中国对虾（Ｐｅｎａｅｕｓｃｈｉｎｅｎｓｉｓ）苗期水质净化剂及辅助饵料，取得了明显效果。育苗池水透明度提高约２０ｃｍ，幼体挂脏率下降５０％，变态提早１ｄ左右，且多产仔虾百万尾     

Effect of copper on the growth of shrimps Litopenaeus vannamei: water parameters and copper budget in a recirculating system

Shrimps( Litopenaeus vannamei) were intensively cultured in a recirculating aquaculture system for 98 days to investigate effects of 0.3 mg/L Cu on its performance, Cu budget, and Cu distribution. Shrimps in Cu-treated systems had greater mean final weight(11.10 vs 10.50 g), body length(107.23 vs 106.42 mm), survival rate(67.80% vs 66.40%), and yield(6.42 vs 5.99 kg/m 3), and lower feed conversion ratio(1.20 vs 1.29) than those in control systems but the differences were not significant. Vibrio numbers remained stable(10 4 –10 6 colony forming units/mL) in the rearing tanks of both control and treated systems. Total ammonium-N, nitrite-N, nitrate-N, pH, chemical oxygen demand, 5-day carbonaceous biochemical oxygen demand, and total suspended solids were similar in controls and treatments. Dissolved Cu concentration in the treated systems decreased from 0.284 to 0.089 mg/L while in the control systems it increased from 0.006 2 to 0.018 mg/L. The main sources of Cu in the treated systems were the artificially added component(75.7% of total input), shrimp feed(21.0%), water(2.06%), and shrimp biomass(1.22%). The major outputs of Cu occurred via the mechanical filter(41.7%), water renewal(15.6%), and draining of the sediment trap(15.1%). The foam fractionator removed only 0.69% of total Cu input. Harvested shrimp biomass accounted for 11.68% of Cu input. The Cu concentration of shrimps in the Cu-treated systems(30.70 mg/kg wet weight) was significantly higher than that in control systems(22.02 mg/kg). Both were below the maximum permissible concentration(50 mg/kg) for Cu in seafood for human consumption in China. Therefore, recirculating systems can be used for commercial on-growing of Litopenaeus vannamei without loss of shrimp quality, even in water polluted by 0.30 mg/L Cu. The mechanical filter is the main route for Cu removal.     

温度和盐度骤变条件下凡纳滨对虾的行为变化研究

以视频记录分析的方法,研究凡纳滨对虾Litopenaeus vannamei在温度和盐度骤变条件下的运动行为、摄食行为及相关生理活动的变化。结果表明,当温度由29℃骤降至18和23℃时,对虾的活动频率、游走速度和颚舟片的摆动频率明显下降,索饵时间显著增加(p<0.05);当温度由29℃骤升至36℃时,对虾的活动频率、游走速度和颚舟片的摆动频率显著升高(p<0.05),索饵时间没有显著差异(p>0.05)。当盐度由30骤降至5和10时,对虾表现出强烈的逃避行为,活动频率、游走速度和颚舟片的摆动频率均显著升高(p<0.05),并且在观察时间内没有发生摄食行为。     

中国对虾血细胞酶细胞化学的初步研究

运用电镜酶细胞化学技术 ,对中国对虾 (Penaeus chinensis)的血细胞和血清中的酚氧化酶、酸性磷酸酶、碱性磷酸酶和过氧化物酶的酶活性部位进行标记。实验结果显示 :酚氧化酶主要以酶原形式存在于大颗粒细胞的颗粒体中 ,酸性磷酸酶和碱性磷酸酶以酶原形式存在于小颗粒细胞的颗粒体中。健康虾和患病虾血细胞中酶活性的表现形式不尽相同 ,在病理情况下 ,颗粒细胞能以细胞解体的方式 ,排出细胞内的颗粒 ,转化成有活性的酶 ,以此提高血淋巴中这几种酶的活性。透明细胞可以吞噬排放颗粒后的颗粒细胞 ,透明细胞中由高尔基体合成的酸性磷酸酶等溶酶体酶主要用于透明细胞的细胞内消化作用。研究认为 :3种血细胞因含有不同的免疫酶以及酶的作用方式不同而具有不同的免疫功能     

中国对虾血细胞及其免疫研究

于１９９１年３月－１９９２年４月青岛近海及一养虾场捕集中国对虾亲虾和越冬亲虾，以扫描电镜和透射电镜观察中国虾的血细胞形态及其亚显微结构；研究定量测定细胞吞噬活性的方法。结果表明，中国对虾血细胞分３种：无颗粒细胞，呈球形或梭形，胞质不含颗粒粒；小颗粒细胞及颗粒细胞，卵球形或椭球球形，胞质含有电子致密颗粒，颗粒可分均匀、非均匀的；小颗粒细胞质富含线粒体，可能与它对异物敏感易发生胞吐作用有关，该细胞是识     

对虾养殖水质与饵料的关系研究

本文报道投喂不同厂家生产的对虾配合饵料致使水质因子变化的比较试验。结果表明：对虾养殖水体的ｐＨ、溶解氧及氨氮含量的变化和饵料的质量有直接关系，且影响到对虾的生存。而养殖水体的盐度、温度的变化和饵料的相关性不甚明显。文中还对配合饵料影响养殖水质因子变化的机理作了初步探讨。     

北方地区秋季日本对虾室内高密度精养的试验

为充分利用每年的8月以后直到翌年3月份都处于休闲状态的育苗场设施资源，笔者于1999年8月在河北黄骅进行日本对虾室内高密度精养的试验。在水温23－28℃、盐度28－32下，8月27日放苗，11月24日收成，养殖时间87d，单位水体产量平均为0．818kg/m^3，平均存活率为50．1％，旬生长率平均为0．75cm，成虾体长平均8cm，饵料系数为2．51，投入与产出比为2．5，平均单位水体创利63．83元／m^3，其结果说明本养殖模式不仅是可行的，而且具有利用率和效益高、实用性强的优点。本文还就相关问题进行了讨论。     

养殖中国对虾肝胰脏细小病毒病的免疫金银染色法诊断研究

80年代以来 ,肝胰脏细小病毒病在养殖中国对虾中广泛流行并造成了一定的损失。作者采用免疫金银染色 (immunogold silver staining,IGSS)技术 ,对患病对虾肝胰脏中的细小病毒(Hepatopancreatic parvovirus,HPV)进行了免疫组织化学的研究。结果表明 ,经 IGSS染色的病虾肝胰脏上皮细胞核内出现了黑色的、圆形或椭圆形、大小为 3～ 15μm的结构。由于该结构与 H- E染色切片上出现的肝胰脏细小病毒包涵体在发生部位、形态和大小上皆一致 ,故而认为该结构为HPV包涵体。证明 ,免疫金银染色法是一种可应用于中国对虾肝胰脏细小病毒病诊断的有效方法。