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101.
Phytoplankton pigment patterns and community composition were investigated in the northern South China Sea using high-performance liquid chromatography and the CHEMTAX software from February 11 to 23, 2009. We recognized four different vertical distribution patterns of pigments: chlorophyll a (Chl a)-like type, divinyl chlorophyll a (DV Chl a) type, even distribution type, and surface type. The average value of ratios of accessory photo-protective pigments (APP) to accessory photo-synthetic pigments was 0.8... 相似文献
102.
高效液相色谱法检测水产品中的孔雀石绿和结晶紫 总被引:3,自引:0,他引:3
样品中的孔雀石绿、结晶紫经试剂盒提取,浓缩后用经固相萃取柱净化、硼氢化钾还原、反向色谱柱分离,使用荧光检测器检测,孔雀石绿、结晶紫的加标回收率在76.1%~92.5%之间,相对标准偏差1.9%~5.8%,检出限均为0.5μg/kg。结果表明:该方法检测孔雀石绿(MG)、结晶紫(GV)处理简单,灵敏度高,节省时间,可用于大量样品的快速分析。 相似文献
103.
Oscar Vicente Monica Boscaiu Miguel ngel Naranjo Elena Estrelles Jos María Bells Pilar Soriano 《Journal of Arid Environments》2004,58(4):705
General responses to salt stress have been investigated in the halophyte Plantago crassifolia. Seed germination was strongly inhibited by NaCl, although seed viability and germination capacity were not affected by salt pre-treatments. A concentration-dependent inhibition of plant growth was observed in the presence of NaCl, which was accompanied by the accumulation of Na+ ions in the leaves, as determined by cation exchange HPLC. A 20-fold increase of proline content in leaves was observed when plants were treated with 500 m
NaCl, suggesting a protective role against high salinity stress for this amino acid, whose possible mechanism of action is discussed. 相似文献
104.
《Limnologica》2017
The colour of the ventral, anal and caudal fins of Eurasian perch can range from pale yellow to intense red. Within spatially separated populations, however, individuals are usually very uniform in colour. We fed astaxanthin- and canthaxanthin-enriched dry feed to juvenile perch from a yellow-finned and a red-finned population to compare the influence of dietary carotenoids on fin colour between these two populations. In this way we wanted to test whether fin colour in perch is predominantly a phenotypically plastic trait or whether differently coloured individuals represent colour morphs. The ventral fins of perch from the red-finned population always exhibited significantly more intense redness when their feed was supplemented with either one or both carotenoid additives. Perch from both populations deposited more canthaxanthin in their ventral fins than astaxanthin, red-finned perch in particular. Yellow-finned perch probably converted canthaxanthin into β-carotene, which was the dominant carotenoid in their ventral fins, whereas the fins of red-finned perch contained only trace amounts of β-carotene. We conclude that these two populations represent colour morphs that differ fundamentally in their ability to metabolise and deposit dietary carotenoids into their ventral fins. Considering the multiple physiological functions of carotenoids, these fundamental differences in carotenoid metabolism between perch colour morphs may have far-reaching consequences for the performance of different populations, for example in their response to parasite infections. 相似文献
105.
利用碱性水解—高效液相色谱联用技术测定了云贵高原四个湖泊沉积物中色氨酸的含量。讨论了色氧酸的早期成岩作用并与其它氨基酸进行比较。利用优化的碱性水解条件(120℃、4.2MNaOH溶液中水解20h)分析沉积物中的色氧酸含量,回收率达93%。实验结果显示,在所研究的四个湖泊沉积物中,色氨酸只是一种微量氨基酸组分,浓度为0.15~2.66μmol/g,占总可水解态氨基酸的0.17%~9.18%。色氨酸的相对摩尔百分含量随沉积深度的增加呈上升趋势,与其它两种芳香性氨基酸酪氨酸和苯丙氨酸的变化趋势相反,揭示了芳香性氨基酸在早期成岩过程中的地球化学行为。研究结果还表明,色氨酸在沉枳物早期成岩过程中被硅藻细胞壁选择性保存。 相似文献
106.
Normal-phase HPLC using a cyano/amino bonded silica stationary phase can provide reliable and rapid fractionation of total neutral fractions from oils and sediments. Separation of aliphatic from aromatic hydrocarbons is possible, but in addition HPLC is able to fractionate aromatic hydrocarbons according to their number of double bonds.Applications of the HPLC fractionation in organic geochemistry are described and illustrated by GC-FID and GC-MS data from an oil and a recent sediment exhibiting a high degree of petrogenic contamination. Alkylphenanthrenes can be obtained free of alkyldibenzothiophenes, allowing evaluation of the methylphenanthrene index by GC-FID without the need for quantification of co-eluting sulphur compounds by GC-FPD.The choice of capillary GC stationary is also considered, particularly with regard to resolution of aromatic steroidal hydrocarbons and alkylphenanthrenes. 相似文献
107.
Pigment signatures of some diatoms isolated from China seas 总被引:1,自引:0,他引:1
1 Introduction Bacillariophyceae (commonly referred to as di- atoms) are a major component of the world’s marine phytoplankton pool, and they form the base of the food chain in many oceanic regions, especially in temperate seawaters (Llewellyn and Gibb, … 相似文献
108.
本研究利用高压液相色谱法(HPLC)法测定:外源睾酮(T)和甲基睾酮(MT)(10μg/g)腹腔注射给入真鲷(Pagrosomusmajor)鱼体后,在血液中的代谢状况.证明,T和MT的吸收和清除很快。MT在注射1h达最大吸收峰(283ng/ml),24h内很快降低。T的吸收和清除状况与MT相似,不同的是其吸收值(最大值为64ng/ml)比MT低许多。随着鱼类养殖至商品规格,类固醇激素的含量将降至很低,因此,MT可做为一种生长促进因子用于水产养殖生产。 相似文献
109.
110.
本文建立了测定海力特胶囊含量和体外溶出度的高效液相色谱方法。采用 Aminex HPX-87H(30 0 mm× 7.8mm)柱 ,以 0 .0 0 2 5 mol· L-1H2 SO4 为流动相 ,柱温为 6 5℃ ,流速为 0 .5 m L·min-1,以示差检测器检测 ,外标法峰面积定量。本法测定线性范围为 1μg~ 4 0μg,方法平均回收率为96 .1% ,RSD为 0 .83% (n=5 ) ,体外溶出度测定结果为 96 %~ 99%。本法测定海力特胶囊含量和溶出度 ,具有方法简便、快捷 ,重复性好的特点 ,可作为制剂质量控制方法。 相似文献