双偏振相控阵雷达与业务雷达的定量对比及观测精度研究

相控阵天气雷达突破了全机械驱动天线天气雷达的时空分辨率瓶颈,能够提供更加快速、精细的观测资料.但阵列天线存在性能参数随扫描角偏离法向而恶化的情况,使相控阵雷达定量测量存在困难.本文针对中国华南地区最新布网的双偏振相控阵天气雷达,通过与当地S波段业务雷达在相同区域内的定量对比,评估了反射率因子差分反射率因子的误差量级及其...     

断陷盆地缓坡带河流沉积砂体定量表征及控制因素分析:以霸县凹陷文安斜坡东营组三段为例

断陷湖盆缓坡河流成因砂体是重要的油气储集单元.根据岩心观察、钻井岩/电特征并结合地震沉积学方法,分析断陷湖盆缓坡河流沉积体系和砂体时空分布特征,能为油气精细勘探提供可靠的依据.研究表明,霸县凹陷文安斜坡中部东营组三段周期性地发育4条呈NE-SW辫-曲复合型河流沉积,由河道沉积、砂坝沉积和泛滥平原沉积3种亚相以及辫状河道...     

Sequence analysis and quantitative detection of Norwalk-like viruses in cultured oysters of China

We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future.     

基于遥感的城市景观生态环境格局分析

借助RS和GIS技术,获取研究区城市的景观生态格局指标,运用定量分析的方法对各种生态类型在城市化进程中对景观格局的作用进行了研究。揭示了部分生态类型持续减少将对生态平衡造成破坏的情况,为城市的职能部门提出生态环境建设的建议和措施。     

马氏珠母贝TRACP基因的克隆及组织表达

【目的】克隆马氏珠母贝(Pinctada martensii)抗酒石酸酸性磷酸酶(Tartrate resistant acid phosphatase,TRACP)基因,分析该基因在不同组织中的表达模式。【方法】用RACE技术克隆得马氏珠母贝TRACP基因(PmTRACP),用实时荧光定量PCR分析该基因在外套膜、闭壳肌、足、性腺、珍珠囊、肝胰腺和鳃中的表达。【结果与结论】PmTRACP基因长度为2 034 bp,开放式阅读框972 bp,编码323个氨基酸,5′UTR长度为27 bp,3′UTR长度为1 035 bp。预测PmTRACP分子质量约为36.39 ku,理论等电点为5.97。该基因含有一个钙调神经磷酸酶样磷酸酯酶结构域。PmTRACP与其他物种TRACP的同源性为48%～65%,与长牡蛎(Crassostrea gigas)TRACP的同源性最高,同时D³²、D⁷⁰、Y⁷³、N¹⁰⁸、H²⁰³、H²¹²、H²³⁷、H²³⁹等8个氨基酸活性位点和N¹¹⁴糖基化位点在不同物种TRACP中高度保守。PmTRACP与长牡蛎TRACP的亲缘关系最近。PmTRACP在马氏珠母贝各个组织均有表达,且在肝胰腺和珍珠囊中高表达。     

Peaks of People: Using Topographic Prominence as a Method for Determining the Ranked Significance of Population Centers

This article borrows a statistical method from physical geography—topographical prominence—to suggest a new technique for measuring the relative significance or rank of population centers. Unlike raw population measures, prominence gives consideration to both the spatial intensity of concentrated population areas as well as the spatial dependence or independence of neighboring settlement clusters in relation to one another. We explain how to apply the topographic prominence calculation method to gridded population data and examine its practical utility through case studies of several U.S. states. We then discuss some ways in which parametric choices about point-to-surface transformations can result in considerably different outcomes and offer further suggestions for conceptualizing and measuring population center significance.     

青蛤(Cyclina sinensis)IKK基因的克隆及其在鳗弧菌(Vibrio anguillarum)刺激下的表达分析

利用构建的青蛤(Cyclina sinensis)转录组文库,筛选到青蛤IKK基因的类似序列。经设计引物克隆比对后确认为CsIKK基因。利用生物信息学软件在线对该基因进行结构分析。采用PCR技术克隆基因,并使用实时荧光定量PCR技术克隆得到CsIKK基因在青蛤五个不同组织中的表达情况及在鳗弧菌(Vibrioanguillarum)的刺激下IKK基因在青蛤血淋巴中的时序性表达情况。综合结果得到, CsIKK基因序列开放阅读框长2298bp,编码765个氨基酸。IKK基因在青蛤的血淋巴、外套膜、闭壳肌、肝脏、性腺和鳃六个组织中均表达,在血淋巴中表达量最高。青蛤IKK基因在鳗弧菌胁迫下表达量在6h时达到最大值,与对照组相比差异极显著(P0.01),表明该基因所指导的蛋白是青蛤重要的免疫信号通路蛋白。     

全国重要矿产总量预测方法

全国重要矿产资源评价涉及25种重要矿产的近百种矿床类型,需要在1∶20万尺度水平圈定成矿预测远景区,并科学估算各远景区资源量,为国家矿产资源战略勘查和战略部署提供技术支撑。为了保证预测成果在同一层面上进行全国汇总,制定了全国一致的、标准的技术要求和方法。通过对中国以往一轮、二轮区划预测方法、全国矿产资源总量预测方法及国外最新预测方法的分析和总结,结合近年来预测理论的新发展,确定了全国重要矿产总量预测的理论基础是成矿系列理论、现代成矿动力学理论和综合信息矿产预测理论,采用的预测思路是矿床模型综合地质信息预测方法。文章陈述了总量预测的方法流程和预测技术要求,有关方法思路可供资源评价人员参考。     

江苏省夏季最高温度定量预报方法

以江苏省徐州、南京、射阳3个探空站2002～2006年7～8月逐日观测资料为基础,选取了影响最高温度变化的因子,利用逐步回归方法建立了以徐州、南京、射阳3地为中心的区域预报模型,并对模型的回归效果和预报情况进行分析.剖析了其用于实际预报的合理性和可信度,同时与欧洲中期天气预报中心动力数值预报结果相结合,利用高斯权重插值方法将预报场的格点资料捕值到江苏各站点,通过PP法,完成了江苏省最高温度的定量预报.预报当天最高温度误差在1℃以下的概率为5O%左右,2℃以下概率在8O%左右,该方法可用于最高温度预报.     

区域生态安全动态变化及空间差异定量分析:以陕北黄土高原为例

1 Introduction Ecological security is defined as the goal of stakeholders to create a condition where the physical surroundings of a community provide for the needs of its inhabitants without diminishing its natural stock (Chen, 2002; Wachernagel et al.,…