若干水华相关藻类对太湖水体异味物质贡献的初步研究

太湖水体中嗅味物质2-甲基异莰醇(MIB)和土臭素(Geo)的出现与水华发生在时间上高度重叠,为探寻水华中常见藻类与嗅味的关系,本研究通过对实验室培养藻株和野外水样比较分析,探寻了部分藻株与太湖水体嗅味物质的关系.分析实验室培养的15株蓝藻(其中11株微囊藻)、4株绿藻和4株硅藻,仅硅藻培养物测定出了Geo,所有藻株均未检测出MIB;对太湖典型水样分析结果显示,水体中MIB与Geo的浓度与微囊藻细胞浓度无相关性;实验室模拟微囊藻水华腐败结果显示,无论是好氧还是厌氧条件下均未产生MIB和Geo;这些数据结果说明湖水中MIB和Geo与水华主要种群微囊藻无直接关系.在鱼腥藻水华中测出了高浓度的MIB,周年水样分析结果显示鱼腥藻细胞数与MIB浓度变化规律一致,因此鱼腥藻可能是MIB的重要来源.但实验室培养的Anabaena sp.PCC7120无论是在缺氮还是有氮培养条件下均不产MIB和Geo,说明嗅味物质的产生具有藻株特异性.     

水华和赤潮的毒素及其检测与分析

本文介绍了水华和赤潮藻类各种毒素的类型及特点,对近年来毒素检测分析方法进行了综述,重点介绍微囊藻毒素（Microcystin,MCYST,MC）和麻痹性贝毒（Paralytic shellfish poisoning,PSP）的提取、检测方法。     

超声波和改性粘土集成技术在去除蓝藻水华上的应用

近年来,改性粘土除藻技术广泛应用于"水华"的治理当中,其原理是藻类与改性粘土絮凝后自然沉降.通过对铜绿微囊藻(Microcystis aeruginosa)、集胞藻(Synechocystis sp.)和小球藻(Chlorella vulgaris)的絮凝比较,发现具气囊的铜绿微囊藻比其他两种藻发生再悬浮的幅度更大,次数更多.针对我国的水华藻类是以微囊藻(Microcystis sp.)等为主的情况,研究超声波和改性粘土集成技术对藻类的去除效果.结果表明,超声波和改性粘土集成技术能将藻类去除率明显提高,该法的絮体稳定性比单一絮凝法明显增强,且对群体形态的藻的去除率提升效果更好.另外,在40kHz、160W超声辐照下,铜绿微囊藻的气囊去除率在95%以上,但细胞壁保持完好,细胞活性不变,藻毒素不会因细胞破裂而释放,因此超声波和改性粘土集成方法,是治理我国蓝藻水华的有效方法.     

基于突变理论的太湖蓝藻水华危险性分区评价

蓝藻水华暴发是湖泊生态系统中营养物质长期累积的结果,是系统营养经长期演化后的极端状态.突变理论评价方法无需确定指标权重,减少了人为主观因素,并且计算方便.本文基于突变理论,采取蓝藻水华暴发的表征因子(叶绿素浓度)和导致蓝藻水华暴发的环境因子(总氮和总磷)作为潜在危险性评价指标,蓝藻水华的面积、范围以及暴发频次作为历史危险性评价指标建立多准则蓝藻水华暴发风险评价指标体系,并结合太湖九个分区进行蓝藻水华暴发危险性分区及全湖评价.研究结果表明:竺山湖和西部沿岸为极重危险性湖区;梅梁湾为重度危险性湖区;南部沿岸、贡湖和大太湖为中度危险性湖区;箭湖东茭咀、东太湖和胥湖蓝藻水华暴发危险性较小,为轻微危险性湖区.整体上看,太湖蓝藻水华暴发危险性程度由轻到重基本上沿东南-西北方向变化,与营养盐浓度由低到高分布趋势相一致.根据评价结果,可以明确太湖各区遭遇蓝藻水华暴发危险性的大小,为蓝藻水华风险管理和应急处理提供科学依据.     

水网藻(Hydrodictyon reticulatum)水华在鄱阳湖的纪录

水网藻水华在国外出现较多,在国内鲜有报道.2013年5月对鄱阳湖进行采样时首次发现水网藻水华;结合现场观测及遥感影像确定其分布范围为老爷庙至落星墩水域,分布面积约为26 hm²,推测水网藻可能是由上游稻田等静止水体迁移至鄱阳湖,在相对静水区进行聚集生长,加上合适的理化条件(温度及营养盐),从而形成水华.在此基础上,本文进一步分析了水网藻水华对鄱阳湖水生态环境可能产生的影响.     

吞噬微囊藻的鞭毛虫的培养

从一个有水华发生的池塘里采集分离到一种能吞噬微囊藻的动基体目鞭毛虫(Kinetoplastida),存光学显微镜及激光共聚焦显微镜下观察了该鞭毛虫的形态特征,初步探讨了它的培养方法.该鞭毛虫以集胞藻、微囊藻、鱼腥藻、小球藻以及蛋黄、奶粉、大肠杆菌和酵母为食物进行培养,结果集胞藻、微囊藻、奶粉、蛋黄、及酵母都是该鞭毛虫很好的食物.该鞭毛虫易于培养,繁殖迅速,培养成本低廉,有应用于生态环境中控制微囊藻水华的可能.     

北京城区河湖水质分析

调研结果显示,2003年北京城区河湖(11个监测水体)总磷、总氮含量分别为0．142mg/L、1．481mg/L,已达到比较 严重的富营养状态．北京城市河湖属于藻型水体,初级生产力主要决定于浮游藻类的群落结构与密度．河湖水体中浮游 藻类密度为37867．82×10~4cells/L,其群落由蓝藻(Cyanophyta)、绿藻(Chlorophyta)、硅藻(Bcillariophyta)、甲藻(Pyrrophy— ta)、隐藻(Cryptophyta)、黄藻(Xanthophyta)、金藻(Chrysophyta)和裸藻(Eugleniphyta)构成．群落中蓝藻占绝对优势 (89．54%)．在近几年的夏秋季连续发生程度不同的微囊藻(microcystis)水华,对水体功能和城市景观造成了不良影响． 主要原因是：(1)氮磷和有机物的污染,(2)给城市河湖补给的水量少,(3)河湖生态系统被损害,水体自净能力差．本文 对如何改善北京城市河湖水质提出了建议．     

秦皇岛入海河流的污染水平、排放特征及对褐潮暴发区生源要素的影响

为查明秦皇岛近海大面积褐潮连年暴发的成因,在2013年3—11月对该地区主要入海河流和沿岸褐潮暴发区的生源要素污染进行了连续调查研究。调查结果表明,所调查入海河流普遍为劣V类地表水,总氮(TN)超标严重,按照氮污染程度由高到低排列依次为大蒲河洋河戴河石河汤河东沙河。各河流中的碳、氮、磷、硅等污染物浓度在时间变化上没有统一规律。基于综合污染指数法的评价结果显示,大蒲河和洋河为重度污染,戴河和汤河从先前的轻度污染加重为中度污染。从污染物入海量上看,TN和化学需氧量(COD)是排放量最高的两种河源污染物,其中溶解态氮占TN的74.6%。在所调查河流中,洋河和大蒲河分别贡献了TN的38.2%和33.2%,同时大蒲河还贡献了75.8%的活性磷酸盐和37.7%的活性硅酸盐,而75.9%的COD来自汤河、洋河和大蒲河。秦皇岛河源污染物排放在时间上较为集中在6—9月的丰水期,但各河流单独的排放具有随机性,没有统一的季节性规律,表现出受人为调控影响明显的特点。秦皇岛沿岸褐潮暴发区的生源要素变化与河源污染物排放有显著性相关(P=0.05)。     

春季长江口富营养化水域浮游植物群集对冲淡水和上升流的响应

A comprehensive study on the phytoplankton standing stocks, species composition and dominant species in the eutrophic Changjiang(Yangtze River) Estuary(CE) was conducted to reveal the response of phytoplankton assemblage to Changjiang Diluted Water(CDW) and upwelling in the spring. Phytoplankton presented peak standing stocks(13.03 μg/L of chlorophyll a, 984.5×103 cells/L of phytoplankton abundance) along the surface isohaline of 25. Sixty-six species in 41 genera of Bacillariophyta and 33 species in 19 genera of Pyrrophyta were identified, as well as 5 species in Chlorophyta and Chrysophyta. Karenia mikimotoi was the most dominant species, followed by Prorocentrum dentatum, Paralia sulcata, Pseudo-nitzschia delicatissima and Skeletonema costatum. A bloom of K. mikimotoi was observed in the stratified stations, where the water was characterized by low nitrate, low phosphate, low turbidity, and specific ranges of temperature(18–22 °C) and salinity(27–32). K.mikimotoi and P. dentatum accumulated densely in the upper layers along the isohaline of 25. S. costatum was distributed in the west of the isohaline of 20. Benthonic P. sulcata presented high abundance near the bottom,while spread upward at upwelling stations. CDW resulted in overt gradients of salinity, turbidity and nutritional condition, determining the spatial distribution of phytoplankton species. The restricted upwelling resulted in the upward transport of P. sulcata and exclusion of S. costatum, K. mikimotoi and P. dentatum. The results suggested that CDW and upwelling were of importance in regulating the structure and distribution of phytoplankton assemblage in the CE and the East China Sea.     

Bottom-up control of phytoplankton growth in spring blooms in Central Yellow Sea,China

The source and significance of three nutrients – nitrogen, phosphorous and silicon – were investigated by a modified dilution method performed on seawater samples from the Central Yellow Sea (CYS), in spring blooming period of 2007. This modified dilution method accounted for the phytoplankton growth rate, microzooplankton grazing mortality rate, the internal and external nutrient pools, as well as nutrients supplied through remineralization by microzooplankton grazing. The results indicate that phytoplankton growth during the bloom is mostly contributed by internal nutrient pools (K_I=0.062–1.730). The external nutrient pools (K_E=<0–0.362) are also of importance for phytoplankton growth during the bloom at some sampling sites. Furthermore, the contribution of the recycled-nutrient pool by remineralization (K_R=<0–0.751) is significant when microzooplankton grazing rate was higher than 0.5 d⁻¹ during the spring phytoplankton blooms in the Central Yellow Sea. Compared with internal phosphorus, internal nitrogen and silicon contribute more to the phytoplankton production at most sampling stations.