Species delimitation in the green algal genus Codium (Bryopsidales) from Korea using DNA barcoding

Codium, one of the largest marine green algal genera, is difficult to delimit species boundary accurately based on morphological identification only. DNA barcoding is a powerful tool for discriminating species of seaweeds. The plastid elongation factor TU(tuf A) is considered as maker to perform DNA barcoding of green algal species than rbc L gene due to universality and rapid evolution rate. We conducted DNA barcoding application to Codium specimens from the Jeju Island, Korea to overcome the limit of morphological identification and to confirm the species diversity. As a result of applying tuf A marker, we newly generated fifty-five tuf A barcodes to resolve eight species. Tuf A marker exhibited 6.1%–21.8% interspecific divergences, wider than the gap of rbc L exon 1,3.5%–11.5%. Molecular analysis of rbc L exon 1 sequences of Codium revealed eight distinct species like tuf A analysis separated in five phylogenetic groups. DNA barcoding of the genus Codium using tuf A marker is more helpful to overcome the limit of morphological identification, and this is more potential to reveal cryptic species and to resolve the relationships among subspecies than rbc L analysis alone. The complement of tuf A barcoding and rbc L analyses including morphology for the genus Codium in the northwestern Pacific will give much more reliable achievement for discovering species diversity and resolving the phylogenetic relationships.     

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桑托斯盆地L构造Aptian期火山喷发旋回特征与Itapema组介壳灰岩沉积

桑托斯盆地裂陷初期岩浆活动与储层发育关系密切。本文以盆地L区块火成岩-介壳灰岩复合体为研究对象,通过对火成岩岩石学、年代学测试及钻井、测井资料分析等,首次明确了桑托斯盆地裂陷初期岩浆活动特征及其对介壳灰岩沉积过程的控制,并指出介壳灰岩储层的分布特征。L区块内火成岩分属晚白垩世Santonian期—Campanian期和早白垩世Aptian两期岩浆活动产物,并以Aptian期为主,至少经历了7次不连续喷发和3次侵入过程,形成了块状玄武岩、杏仁状玄武岩和玻基斑状玄武岩(含再沉积玻基斑状玄武岩)等喷发相火成岩和以辉绿岩(局部属粗玄岩)、煌斑岩形式产出的侵入相火成岩。Aptian期岩浆多期活动重塑了古地貌,形成了局部构造凸起,促成并控制了下白垩统Itapema组至少6期介壳灰岩沉积,最终形成了～620 m厚火成岩-介壳灰岩复合体。介壳灰岩储层呈透镜体形状,以“散点式”不连续分布在Aptian期盆内古隆、古断阶(坡)等区域,横向连续性差,垂向连通性受限,其发育规模和质量受古地貌及水深条件等影响。     

基于滑动L2优化准则的GPS坐标时间序列重构

GPS坐标时间序列中不仅包含白噪声,还包含闪烁噪声、随机漫步噪声等有色噪声,这些噪声将影响GPS应用的可靠性,甚至可能对一些地球物理现象做出错误的解释,因此降低GPS坐标时间序列中有色噪声的影响、提高GPS精度是一个重要和基本的问题。提出了一种滑动L₂优化估计方法(ML₂),通过选取合适的窗口建立L₂优化模型,再利用交替迭代乘子法求解每段时间序列的优化问题,并逐年滑动得到整段GPS坐标时间序列的估计。实验结果表明,ML₂方法与奇异谱分析、小波分解、滑动普通最小二乘法相比具有更好的重构效果。     

马氏珠母贝(Pinctada fucata)组织蛋白酶L基因的克隆与表达分析

根据已构建的溶藻弧菌(Vibro alginolyticus)诱导的马氏珠母贝(Pinctada fucata)血淋巴cDNA差减文库得到的ESTs序列, 应用cDNA末端快速扩增(RACE)技术成功克隆了其组织蛋白酶L基因(PFCatL), 并对其进行了生物信息学分析; 应用实时荧光定量PCR (Real-time PCR)技术, 研究了PFCatL基因在溶藻弧菌刺激前后马氏珠母贝足、外套膜、鳃、闭壳肌等8个组织中的表达变化。结果表明, PFCatL基因cDNA全长2004bp, 其中5′非编码区(5′-UTR)50bp, 3′非编码区(3′-UTR)865bp, 开放阅读框(ORF)1089bp, 编码362个氨基酸, 其分子量计算值(MW)为40.52kDa, 理论等电点(IP)为5.20; 生物信息学分析表明, PFCatL含有16个氨基酸残基组成的信号肽序列以及组织蛋白酶前体抑制功能域I29; Clustalw2多重比对发现PFCatL氨基酸序列在催化三联体Cys-His-Asn、底物结合位点以及二硫键形成相关的半胱氨酸残基位点高度保守; Real-time PCR研究发现, PFCatL在马氏珠母贝各组织中均有表达, 但各组织间的表达量存在差异, 其中以肾和闭壳肌中的表达量最高; 溶藻弧菌感染4h后, 外套膜、鳃以及血淋巴中PFCatL基因的表达较感染前显著上调。     

一类信号恢复问题的投影迭代硬阈值算法

本文给出了基于L₀模求解该问题的非凸模型,借助于稀疏正则化方法来克服问题的不适定性。该模型利用紧小波框架对信号进行稀疏逼近,并利用L₀模度量稀疏性。提出了求解该模型的投影迭代硬阈值算法,并证明了算法的全局收敛性。该算法每一步都有闭式解,计算过程简洁高效。数值实验表明,方法在重建信号的视觉质量和量化指标方面均优于所对比的pFISTA方法。     

褐藻纲海带目Laminaria属和Saccharina属基于三个分子标记的系统发育分析

In the past two decades, many studies have focused on the classification within genus Laminaria, ultimately trying to divide it into two subgroups or genera: Laminaria and Saccharina. A significant debate still surrounds the question of its division, as the conflicting phylogenetic hypotheses that have resulted from the classification studies are based on different taxon sampling, molecular markers, or analysis methods. It is aimed at elucidate the molecular phylogeny within Laminaria and Saccharina. The nine species of Laminariales are sampled from northern Asia and Europe, and 23 new sequences in the nuclear, plastid, and mitochondrial genomes are determined to identify their taxonomic status. The phylogenetic analyses of 71 species are performed, including representatives from six of the seven families of the order Laminariales, based on three separate data sets. An evidence is provided to strongly support a clear split that maintains the two recognized genera, Laminaria and Saccharina, with Laminaria appearing to be the ancestor group. Further, analyses indicate that all taxa in Saccharina and Laminaria did not form a monophyletic lineage, instead Laminariaceae and Lessoniaceae grouped together interlacedly, and Costariaceae appeared as the sister taxon of the Lessoniaceae–Laminariaceae clade. In the phylogenetic analysis, mitochondrial c oxidase I(COI) sequences appeared to be the most credible molecular marker which was more befitting than nuclear encoded internal transcribed spacer(ITS) and plastid encoded rbcL for establishment of Laminariales systematics. It is the most comprehensive phylogeny of the order Laminariales, and contributes to an enhanced understanding and estimation of the phylogenetic relationships for the economically important seaweeds, Laminaria and Saccharina.     

主成分分析在航空瞬变电磁去噪中的应用

由于航空瞬变电磁二次场数据是一种宽频带信号且幅度较小,因此常常受到随机噪声、天电噪声以及人文噪声的影响。研究针对上述噪声的去噪方法,可以提高处理和解释的精度。这里给出了基于主成分分析的航空瞬变电磁去噪方法,首先应用主成分分析方法对叠加抽道后的单测点衰减曲线进行分解,然后应用曲线趋势对比法和L曲线法对分解后的成分进行分析,确定出有效信号成分,最后使用这些有效信号成分进行重构,从而达到抑制噪声的目的。模拟信号分析以及实测数据的实验结果表明,应用主成分分析法处理航空瞬变电磁数据,在保持反映地下介质分布的有效信号幅度基本不变的条件下,能有效抑制天电噪声和人文噪声,为保幅处理和解释奠定了良好的基础。     

基于压缩感知原理的L1范数AVO反演算法

基于压缩感知稀疏信号采样与重构理论,利用AVO反演方法将传统的L2范数改变为L1范数,反演地下地层在L1范数下的稀疏脉冲反射系数。反演得到的稀疏尖峰将局部地下结构通过有限数量的层状结构的叠加来表示,能够提高纵向精度,与传统的AVO反演算法相比提高了薄层的反演效果且具有一定的抗噪性。数值模型及实际数据的结果表明,基于压缩感知原理的L1范数AVO反演方法更加准确、分辨率更高。     

用Atmega8L实现无线恒温控制燃气热水器

介绍了无线恒温控制系统的作用和组成、单片机Atmega8L、无线模块nRF905的特点和引脚、步进电机的控制和驱动，热敏电阻的连接以及整个系统的软硬件实现。