四种虾蟹类幼体消化酶活力的比较研究

采用酶学分析方法测定了中国对虾、日本对虾、中华绒螯蟹和三疣梭子蟹各期幼体的４种消化酶活力，并对它们在幼体发育过程中消化酶活力的变化规律进行了分析研究。实验结果表明，４种虾蟹类在整个幼体发育过程中，４种消化酶活力表现出３种不同的变化模式，其中虾类和蟹类幼体消化酶活力的变化模式有各自的相似性；而且它们的胃蛋白酶、类胰蛋白酶和淀粉酶活力较高，纤维素酶活力极微。在食性转换过程中，胃蛋白酶、类胰蛋白酶和淀粉酶活力出现较明显的变化。同时还探讨采用淀粉酶／类胰蛋白酶活力（Ａ／Ｔ）比值作为甲壳动物幼体的食性指标     

休眼与非休眠期中华鳖乳酸脱氢酶同工酶的比较分析

本文用聚丙烯酰胺凝胶电泳的方法，分析了休眠与非休眠期中华鳖10种组织的乳酸脱氢酶同工酶，同时测定了休眠与非休眠期乳酸脱氢酶的总活性。结果表明，某些组织的电泳图谱显示出一定差异；多数组织冬眠期LDH同工酶的总活性有所降低。     

中华鳖气单胞菌疖疮病的可溶性蛋白质分析

本文用聚丙烯酰胺凝胶电泳方法,分析了健康鳖与患疖疮病病鳖4种组织和血浆的可溶性蛋白质。结果表明,中华鳖组织和血浆的蛋白质电泳图谱存在组织特异性;病鳖与健康鳖的蛋白质分离谱带上存在明显差异,主要表现为谱带的增多．     

中华绒螯蟹幼蟹维生素C营养需求研究

在每100g等氮、等能纯化饲料中分别添加0,100,200,400,800,1 000mg维生素C(Vc)多聚磷酸酯(LAPP),用这样配制成的试验饲料饲喂已淡化5d的河蟹大眼幼体及后续幼蟹25d,探讨Vc对大眼幼体和幼蟹生长、存活、蜕壳频率及抗逆性的影响,同时探明幼蟹对Vc的适宜需求量,结果表明,饲料Vc添加量为100~400mg/100g时,可以保证幼蟹正常生长、存活和蜕皮,尤其以投喂添加Vc200mg/100g(C₂组)和400mg/100g(C₃组)饲料的Ⅲ期幼蟹增重率、存活率和平均蜕皮频率较高,其中C₂组(上述各指标值)分别为516.18%,62.83%,2.58,C₃组分别为458.89%,66.17%,2.61;饲料中缺少Vc时(如C₀组),Ⅲ期幼蟹的增重率、存活率和平均蜕皮频率最低,分别为265.31%,26.67%和2.26.饲料中Vc的添加量达到800mg/100g(C₄)和1 000mg/100g(C₅)时,幼蟹的蜕皮反而受到抑制,平均蜕皮频率降低,蜕皮周期延长,且存活率降低,到Ⅲ期幼蟹的增重率、存活率和平均蜕皮频率在C₄组分别为355.7%,31.33%,2.39,在C₅组分别为307.53%,28.56%,2.19.此外,各处理组幼蟹对抗逆性影响的试验表明,在一定的范围内,饲料中添加Vc能提高幼蟹的耐低氧能力和耐pH突变能力,而对耐盐度突变能力没有明显的影响.综合上述结果可以得出,为保持幼蟹良好生产性能,饲料中添加的Vc?     

真菌在尼泊尔喜马拉雅地区基于生态系统的适应（EbA）实践中的作用评估（英文）

为了实现可持续发展目标（SDG）,从而实现2020年后全球生物多样性目标并提高人类社会对气候变化的抵御能力,建议将基于生态系统的适应（EbA）等基于自然的方法作为一种有前途的综合适应战略。EbA包括重视生态系统在降低社会对气候变化的脆弱性方面的作用的适应战略。在地球上不同的生物群中,真菌不仅在维持生态系统中的生物地球化学循环/养分循环（支持和调节服务）方面发挥着重要作用,而且还为人类的社会经济和文化利益（供应和文化服务）做出了贡献。本文根据对冬虫夏草（Ophiocordyceps sinensis）和其他野生真菌的实地考察、相关文献回顾以及作者的相关专业知识,对这些生物多样性真菌群体如何对基于生态系统的适应（EbA）方法的知识的科学理解进行了介绍。在尼泊尔,很多当地社区将几种真菌用作食品、药品,真菌同时也是尼泊尔山区社区的重要家庭收入来源,可在灾难期间提供缓冲,并对粮食安全、医疗保健、教育等具有积极作用。对于综合EbA方法,应加强地方机构的管理以及本土（地方）知识的教育,以对生态、社会和经济有用的真菌物种的识别、保护和可持续管理进行适当的政策干预。     

GENETIC DIFFERENTIATION WITHIN ERIOCHOIR SINENSIS(MILNE,EDWARDS) REVEALED BY ALLOZYME AND RAPD

ＩＮＴＲＯＤＵＣＴＩＯＮＥｒｉｏｃｈｏｉｒｓｉｎｅｎｓｉｓｉｎＣｈｉｎａｉｓｃｏｍｍｏｎｌｙｃａｌｌｅｄｍｉｔｔｅｎｃｒａｂ .Ｉｔｉｓａｎａｄｒｏｍｏｕｓ,ｗｉｔｈｉｎｄｉｖｉｄｕａｌｓｍｏｖｉｎｇｆｒｏｍｆｒｅｓｈｔｏｍａｒｉｎｅｗａｔｅｒｔｏｓｐａｗｎ .Ｔｈｅｒｅａｒｅｔｗｏｃｏｍｍｅｒｃｉａｌｐｏｐｕｌａｔｉｏｎｓ:ＣｈａｎｇｊｉａｎｇＲｉｖ ｅｒ (ＣＲ)ａｎｄＬｉａｏｈｅＲｉｖｅｒ (ＬＲ) .Ｔｈｅｓｅｔｗｏｐｏｐｕｌａｔｉｏｎｓａｒｅｖｅｒｙｄｉｆｆｉｃｕｌｔｔｏｉｄｅｎｔｉｆｙｆｒｏｍｔｈ…     

First Discovery of Dicerorhinus sumatrensis from Yanjinggou Provides Insights into the Pleistocene Rhinocerotidae of South China

The Rhinocerotidae is one of the most common and important families in the Pleistocene mammalian fauna of South China. Since the last century, most of the Pleistocene rhinocerotid fossils were prematurely assigned to Rhinoceros sinensis, which has resulted in confusion of taxonomy for decades, especially in South China. The Yanjinggou area in Chongqing Municipality of China, where the neotype of R. sinensis came from, has yielded abundant rhinocerotid fossils and is a key area to solve this problem. A recently discovered juvenile skull from a karstic fissure in this area can be referred to Dicerorhinus sumatrensis (Fischer, 1814), which is the first confirmed non-Rhinoceros rhinocerotid from the area. This new finding indicates that non-Rhinoceros fossils might have been mixed in AMNH collection from Yanjinggou and thus the diagnosis of Rhinoceros sinensis needs reformation. On the other hand, Stephanorhinus ?kirchbergensis is also detected in Granger’s collection based on our reobservation. The coexistence of Rhinoceros, Dicerorhinus and Stephanorhinus shows the higher diversity of Pleistocene rhinocerotids in South China.     

中华绒螯蟹精子形成过程酸性磷酸酶的分布特征

将金属盐法与光镜、电镜技术相结合,研究中华绒螯蟹精子形成过程中酸性磷酸酶分布的变化。研究表明,在精子形成的早期,酸性磷酸酶由细胞质中的内质网小泡上产生,随后出现在细胞核内、顶体囊膜上及顶体管内,并且反应产物逐渐由分布均匀的细密颗粒聚集成分布均匀的较大的颗粒;当精子成熟后酸性磷酸酶均匀分布在顶体管中,反应产物颗粒比较大,穿孔器部分发现有少量酸性磷酸酶分布。     

盐度突变对青蛤(Cyclinasinensis)鳃Na~+/K~+-ATPase活性的影响

研究了盐度23下青蛤(Cyclina sinensis)鳃、斧足、外套膜和内脏团中Na+/K+-ATPase的活性差异,并以活性高的鳃组织为对象,对青蛤实施了低盐(23→16)和高盐(23→36)突变的胁迫,监测了鳃组织中Na+/K+-ATPase活性的动态变化。结果表明,盐度23下青蛤鳃组织中Na+/K+-ATPase的活性显著高于其他组织;在盐度突降胁迫下,鳃组织中Na+/K+-ATPase的活性于前16 d逐渐升高,第17d起显著升高(p<0.05),第18 d达到峰值,于第20 d回落,但仍高于起始水平;在盐度骤升胁迫下,鳃组织中Na+/K+-ATPase的活性于前13d逐渐降低,第14 d起显著降低(p<0.05),于第15 d达到最低值,继而回升至平稳水平,但仍低于起始水平。青蛤不同盐度胁迫下其鳃组织中Na+/K+-ATPase活性的响应方式不同,低盐时活性增加,而高盐时活性下降;此外,青蛤在盐度胁迫下对自身渗透压的调节则需较长时间。     

Effects of different dietary lipid contents on growth and lipase activity of Eriocheir sinensis larvae

The effects of different dietary lipid content on the growth and lipase activity of Eriocheir sinensis larvae were studied in the paper. The results showed that the survival, metamorphic rate and weight gain of E. sinensis larvae at different stages of growth all varied significantly with lipid content (P<0.05). Further, the survival and metamorphosis rates were the highest during the larval phases Z₃ to Z₄, and the weight gain was the highest during the larval phases Z₅ to M. During the first 20 h after metamorphosis of every larval stage, the lipase activity increased over time at Z₁, Z₂, Z₃ and M and declined at Z₄ and Z₅, and was influenced significantly by lipid content (P<0.05). In addition, lipase activity at each larval stage began to respond to dietary lipid contents 4 h after the larvae were fed, and tended to be stable after 12 h. The diets with higher lipase activity and lower lipid content were selected to give the suitable recipe of lipid requirements at each larval stage. It was concluded that the suitable lipid requirements at Z₁, Z₂, Z₃, Z₄, Z₅ and M were 6%, 4%, 6%, 8%, 8% and 10%, respectively.