健脾解毒活血汤对幽门螺杆菌相关性胃炎脾胃湿热证模型小鼠IL-1β、GAS的影响

目的：观察健脾解毒活血汤对幽门螺旋杆菌相关性胃炎（HAG）脾胃湿热证模型小鼠血清白介素-1β（IL-1β）、胃泌素（GAS）及胃内组织Hp感染数的影响,为研制新型抗Hp感染的中药提供实验依据。方法：选取40只SPF级昆明小鼠为实验对象,将其随机分为空白组、模型组、西药组和中药组,每组各10只。采用Hp菌液灌胃以建立HAG脾胃湿热证小鼠模型,西药组予以奥美拉唑钠肠溶胶囊灌胃,中药组予以健脾解毒活血汤灌胃,均连续给药14d。观察比较各组小鼠血清IL-1β、GAS与胃内组织Hp感染情况。结果：模型组小鼠血清IL-1β、GAS的含量较空白组明显升高（P<0.01,P<0.05）;与模型组比较,中药组和西药组血清IL-1β显著降低（P<0.05,P<0.01）,GAS水平2组无明显差异。模型组小鼠胃组织Hp感染数较空白组明显增加（P<0.01）,中药组和西药组胃组织Hp感染数较模型组显著降低（P<0.05）。结论：健脾解毒活血汤对HAG脾胃湿热证模型小鼠血清IL-1β及Hp感染数有明显改善作用,但对GAS影响不大,在一定程度上可有效治疗脾胃湿热型HAG。     

Isolation of protein from Chlorella sorokiniana CY1 using liquid biphasic flotation assisted with sonication through sugaring-out effect

Microalgae,a sustainable source of multi beneficial components has been discovered and could be utilised in pharmaceutical,bioenergy and food applications.This study aims to investigate the sugaring-out effect on the recovery of protein from wet green microalga,Chlorella sorokiniana CY1 which was assisted with sonication.A comparison of monosaccharides and disaccharides as one of the phaseforming constituents shows that the monosaccharides,glucose was the most suitable sugar in forming the phases with acetonitrile to enhance the production of protein(52% of protein).The protein productivity of microalgae was found to be significantly influenced by the volume ratio of both phases,as the yield of protein increased to 77%.The interval time between the sonication as well as the sonication modes were influencing the protein productivity as well.The optimum protein productivity was obtained with 10s of resting time in between sonication.Pulse mode of sonication was suitable to break down the cell wall of microalgae compared to continuous mode as a lower protein yield was obtained with the application of continuous mode.The optimum condition for protein extraction were found as followed:200g/L glucose as bottom phase with volume ratio of 1:1.25,10s of resting time for ultrasonication,5s of ultrasonication in pulse mode and 0.25g of biomass weight.The high yield of protein about 81% could be obtained from microalgae which demonstrates the potential of this source and expected to play an important role in the future.     

Growth inhibition and oxidative stress in two species of marine diatoms exposed to 1-phenylethanol

1-phenylethanol (1-PEA) is a flavor extensively used in the production of cosmetics, beverages, and food. The release of 1-PEA into coastal environments has aroused great concern. However, its potential effects on marine organisms are still unknown. In order to provide a better understanding of the ecological risks of 1-PEA in marine environments, this study determined the toxic effects of 1-PEA on two marine diatoms (Phaeodactylum tricornutum and Skeletonema costatum). The diatoms were grown in culture medium containing different concentrations of 1-PEA for 96 h. The contents of chlorophyll a, chlorophyll c, glutathione (GSH), malondialdehyde (MDA), and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), were measured at the end of the exposure period. 1-PEA was shown to significantly inhibit the growth of diatoms, with 96-h EC50 values of 257.14 mg/Land 126.46 mg/L in P. tricornutum and S. costatum, respectively. In P. tricornutum, the levels of SOD, CAT, GPx, GSH, and MDA were stimulated only when 1-PEA concentrations were close to or greater than the 96-h EC50 value. However, in S. costatum, the activities of SOD and CAT, and the syntheses of two chlorophylls were inhibited even at an exposure concentration below the 96-h EC50 value. Taken together, these findings indicate a potential ecological risk by discharging 1-PEA into coastal areas and its species-specific toxic effects on marine organisms.     

EPS脚本在地形图测绘中的应用技巧

随着城市化发展进程的加速以及智慧城市理念的拓展和延伸,对于现代化城市空间信息应用的需求量变得更丰富,更快捷,更高效;相对于数字城市时期的测绘地理信息,现如今对于数据的采集方式、数据整理、数据存储、数据管理等方面提出了更高的要求;在城市大比例尺地形图中的体现更为明显,这就对城市大比例尺地形图测绘提出了新要求,如何更快捷、更高效、更准确的获取、整理及管理测绘数据以供不断提升的使用需求,成了推动测绘技术革新的动力,这其中,各种新技术的应用及新型测绘软件的应用有效的提高了生产效率。     

背负式移动激光扫描系统测绘大比例尺地形图精度试验研究

将背负式移动激光扫描系统应用在测绘大比例尺地形图中,其扫描精度至关重要。本文利用徕卡Pegasus Backpack对苏州工业园园区测绘地理信息大楼进行扫描,采用Inertial Explorer、Infinity、AoTumatic Processing对点云数据进行预处理,运用RealWorks提取特征点,将特征点在MicroStation V8联图中绘制成1：500地形图。通过与传统方法绘制的1：500地形图相叠合,发现两幅地形图具有很好重合度。对地物检测点精度分析后,得到点位中误差为0.026 m,高程中误差为0.041 m。研究结果表明徕卡Pegasus Backpack满足1：500地形图测量精度要求。     

随机模型对解算西安流动SLR站坐标的影响

合理的参数估计及精度评定不仅需要可靠的函数模型,而且需要正确的随机模型。从权函数和粗差编辑两方面,研究了不同随机模型对西安流动卫星激光测距（satellite laser ranging,SLR）站坐标解算的影响,采用全球Lageos-1卫星观测数据计算了西安流动SLR站坐标。计算结果表明:①西安流动SLR站的观测精度和坐标解算精度均达到厘米级。②随机模型直接影响SLR站坐标的解算结果及可靠性;对于相同的计算弧段,抗差方差分量估计得到的站坐标精度最高、结果最稳定,残差加权均方差最小,观测资料利用率也最高;对于相同的计算方案,采用的SLR数据越多,坐标估计精度越高。     

国产卫星在基础地质调查中的适用性对比研究

为了更好地了解国产卫星的应用性能,围绕地矿业务化应用中的关键环节,对比分析了GF-1卫星数据和ZY-1 02C卫星数据在基岩裸露区基础地质调查中的适用性。从业务应用的角度,按照地质调查业务流程以及相关技术规范和指南,重点从信息承载能力、综合制图能力、地质要素识别能力3个方面评价了GF-1卫星数据和ZY-1 02C卫星数据在遥感基础地质调查中的适用性。     

基于时间序列Sentinel-1A数据的水稻识别

基于时间序列的Sentinel-1A数据,利用光谱相似性方法(SSM)对广东省台山县汶村镇和海宴镇进行了水稻识别。首先将SSM应用于时间序列SAR图像中,计算光谱相似度;再通过设置阈值获得初始水稻种植面积图;最后消除斑点噪声,获得水稻种植面积图。结果表明,基于VH极化图像,利用SSM和阈值法获得的水稻种植面积图的总体精度最高为97.34%,Kappa系数为0.94。因此,时间序列Sentinel-1A数据对于识别水稻或其他作物具有很大的潜力。     

地形图分幅编号全球适用性剖析

从工作中遇到的地形图分幅编号问题着手,简要阐述了我国现有地形图分幅编号的情况,分析了不同区域地形图分幅编号的有关问题以及解决对策,并针对全球地理信息资源建设项目中分幅编号问题提出了解决方案,对于实现全球地形图统一分幅编号具有重要的参考价值。     

移动扫描系统在1∶2 000地形图成果检验中的应用

基于移动扫描系统提出一套新的1∶2 000地形图检验流程,通过与全站仪、RTK等传统数学精度检验方式,论证移动扫描系统三维点云数学精度对于1∶2 000地形图精度检测的适用性。通过与传统人工外业巡视等传统地理精度检验方式,对比、分析全景影像对于地理精度检测的辅助作用。实验结果表明,移动扫描系统的三维点云数据数学精度符合1∶2 000地形图测绘成果质量精度检测要求,以全景影像辅助地理精度检查一定程度降低外业检验时长。因此,将移动扫描系统运用于1∶2 000等大比例尺地形图成果质量检验工作能一定程度优化检验流程、提升质检效率,为测绘成果质量检验工作提供新思路。