洪泽湖轮虫群落结构及其与环境因子的关系

2010年5月至2011年2月对洪泽湖轮虫进行季度采样,分析了洪泽湖轮虫的种类组成、时空分布及其群落结构与环境因子的关系.结果表明,洪泽湖共观察到轮虫17属34种,优势种为螺形龟甲轮虫(Keratella cochlearis)、前节晶囊轮虫(Asplanchna priodonta)、长肢多肢轮虫(Polyarthra dolichoptera)、萼花臂尾轮虫(Brachionus calyciflorus)和曲腿龟甲轮虫(Keratella valga).轮虫种类的空间分布格局呈现较大差异,种类数在北部成子湖最高(27种),西南部湖区次之(26种),东部沿岸带最低(19种);而密度则表现为西南部湖区略高于成子湖,东部沿岸带最低.轮虫的群落结构季节差异明显,密度和生物量在春季最高,秋季次之,而夏季最低.典范对应分析结果表明,水温、溶解氧及叶绿素a等因子对轮虫种类的季节变化及密度生物量的影响最大;总磷、总氮及可溶性无机氮等水体中的营养盐也是影响轮虫群落结构的重要因素.     

不同水力调度模式的两座调水型水库轮虫群落结构的比较

为了解水力调度对轮虫群落结构的影响,于2016年8月-2017年7月对调水水源相同而水力调度模式不同的两座水库（直流型水库和中等水力滞留时间水库）的轮虫种类、生物量及环境因子进行逐月观测.两座水库中共鉴定出轮虫37种,常见属为臂尾轮属（Brachionus）、异尾轮属（Trichcerca）、龟甲轮属（Keratella）、三肢轮属（Filinia）和多肢轮属（Polyarthra）,它们的种类数分别占直流型水库和中等水力滞留时间水库所检出轮虫总种类数的58%和64%.两座水库轮虫种类虽基本一致,但轮虫优势种（相对生物量大于10%）有较大差异.直流型水库轮虫优势种以角突臂尾轮虫（Brachionus angularis）、镰状臂尾轮虫（B.falcatus）、裂足臂尾轮虫（B.diversicornis）、剪形臂尾轮虫（B.forficula）、截头皱甲轮虫（Ploesoma truncatum）、尖尾疣毛轮虫（Synchaetidae stylata）和真翅多肢轮虫（Polyarthra euryptera）为主;中等水力滞留时间水库主要以卜氏晶囊轮虫（Asplanchna brightwelli）、裂足臂尾轮虫（B.diversicornis）和镰状臂尾轮虫为轮虫优势种.两座水库Bray-Curtis相异系数为0.42~0.77,表明轮虫群落结构差异较大.冗余分析表明,叶绿素a浓度和温度是影响两座水库轮虫群落结构的重要因素.两座水库轮虫群落结构的Bray-Curtis相异系数与基于叶绿素a浓度和水温的环境欧氏距离呈显著正相关（R_adj²=0.426,P<0.05）.结果表明,虽然具有相同的调水水源,但由于两座水库的水力调度不同,叶绿素a浓度和水温有显著差异,轮虫群落结构趋于相异.     

广州市不同类型水体轮虫群落结构的时空变动及与理化因子间的关系

轮虫是水体中重要的浮游生物类群,对环境变化敏感,是水生态系统中食物链及微型食物网的关键环节.于2015年夏季(7月)和冬季(12月)对养殖池塘、水库、广州城市湖泊、珠江河口及珠江河段水域的轮虫和理化环境指标进行了调查分析,共发现轮虫26属、76种,其中裂痕龟纹轮虫(Anuraeopsis fissa)在7月河流水体占绝对优势,但12月数量明显减少.冬季珠江河段和河口水体群落多样性指数与均匀度指数较夏季高,群落结构较夏季稳定.调查水体轮虫丰度范围为33~2625 ind./L,城市湖泊夏季丰度较高,冬季有所下降,而养殖池塘轮虫丰度在冬季有所上升.群落结构相似性分析(ANOSIM)分析表明,不同类型水体之间差异显著,尤以湖泊与河流差异性最大,广布多肢轮虫(Polyarthra vulgaris)在湖泊贡献率最高,暗小异尾轮虫(Trichocerca pusilla)其次;裂痕龟纹轮虫在河流贡献率最高,优势种丰度差异是造成湖泊与河流群落结构差异的主要原因.统计分析表明,轮虫丰度与叶绿素a浓度呈正相关.冗余分析与ANOSIM分析发现广布多肢轮虫在流花湖等叶绿素a浓度较高的静态水体中易形成优势;裂痕龟纹轮虫和角突臂尾轮虫(Brachionus angularis)在珠江河段等总氮和总磷浓度高的富营养化流动水体中易形成优势.综合轮虫群落结构和水质特征,广州市水体富营养化严重,耐污性轮虫种类多,应加强城市水生态系统保护和管理.     

Temperature Effect on the Life History of Three Types of Brachionus calyciflorus Females

The effect of temperature on the life history characteristics of amictic females ( AF), unfertilized mictic females (UMF) and fertilized mictic females (FMF) in Brachionus calyciflorus was studied with replicated individual cultures at 20℃ ,25℃ and 30℃ , and with algae Scenedesmus obliquus for their food. There were highly significant effects of both temperature and female type, independently and interactively, on the duration of juvenile and post-reproduction periods, and the number of eggs produced by the rotifer per life cvcle. Among all the temperature-female type combinations, all the juvenile periods of FMF, and the post-reproduction periods of UMF and FMF at 20℃, were the longest, and the number of eggs produced by an UMF at 30℃ was the highest. There were highly significant effects of both temperature and female type on the duration of the reproduction period, but no clear correlation was observed between temperature and female type. The reproduction period of AF was longer than that of UMF and FMF.Only temperature influenced significantly the mean life-span of the three types of females. The duration of juvenile,reproduction and post-reproduction periods as well as the life-span of the three types of females were all reduced very significantly with rise of temperature, but the rates of reduction varied with female type. Among the three types of females, the number of eggs produced per life cycle by an UMF was the highest, and that of a FMF was the lowest. A significant relationship between the number of eggs produced per life cycle and temperature was observed only in the UMF.     

STUDY ON MIXIS POTENTIAL OF ROTIFER RESTING EGGS（ BRACHIONUS PLICATILIS ） WITH DIFFERENT COLLECTION TIMES AND DIFFERENT PRESERVATION PERIODS

The present study investigated the possible changes in the mixis potential of rotifer resting eggs produced by a single stock of Brachionus plicatilis and collected and preserved annually from 1985 - 1998. Several clones derived from each batch of resting eggs were cultured under the same conditions for 21 days. The percentage of clones appearing resting eggs and the average yield of resting eggs produced from each clone were recorded and statistically analyzed to find the differences between the mixis potential of those resting egg batches. Results showed that different batches of resting eggs had different mictic levels among their descendent clones; but no regular relationship was found between the mixis potential of resting eggs and their collection times/preservation periods. Several internal and external factors that might affect the mixis potential of resting eggs were discussed.     

秦皇岛海域“褐潮”海水对卤虫、轮虫存活和摄食的影响研究

为评价秦皇岛褐潮现场海水的毒性,作者利用HPLC技术及CHEMTAX,分析了2013年7月2日采自秦皇岛褐潮高峰期微型及微微型浮游植物的色素组成及群落结构;测定了卤虫(Artemia salina)、轮虫(Brachionus plicatilis)存活率和摄食量。研究表明,褐潮海水微型及微微型浮游植物中,海金藻(Pelagophyceae)占主要优势,其生物量占总生物量的72.6%。褐潮海水抑制卤虫存活,卤虫48 h存活率降至77%;对轮虫24 h存活没有显著效应。此外,褐潮海水抑制卤虫、轮虫的摄食,卤虫、轮虫8 h内摄食量分别由(5.5±0.4)ng/个降至(2.4±0.4)ng/个、由(1.5±0.2)ng/个下降至(0.9±0.1)ng/个。该结果与美国的3株褐潮藻的毒性进行了比较。CCMP 1507对卤虫存活有显著抑制作用,其他两株没有显著效应;3株褐潮藻对轮虫24 h存活都没有显著影响。暴露于3株褐潮藻中,卤虫的摄食量均显著较低;其中CCMP 1507在混合饵料中还抑制卤虫、轮虫对其他藻的摄食。对CCMP 1507各组分分析发现,该藻的毒性主要来源于藻细胞。结合现场试验与室内试验结果分析表明,秦皇岛褐潮对卤虫、轮虫有一定的毒性,其毒性与CCMP 1507的毒性相当。     

山东省的淡水轮虫类

轮虫是池塘、湖泊以及水库中主要浮游动物之一,是鱼类,特别是青、草、鲢、鳙四大家鱼的仔鱼及成鱼的良好食物,对于淡水养殖业有较大的影响。轮虫在淡水生物界的食物链中占重要的位置,它以水体中的微生物和有机碎屑为食,并能以孤雌生殖的方法不断地进行     

用硫酸铜杀灭对虾养殖池内轮虫和原生动物的试验

随着对虾养殖业不断发展,海水富营养化日趋严重,致使虾池水中轮虫密度剧增导致对虾浮头死亡,损失极大。例如1984年乳山县,在8月中旬虾池内轮虫密度达50个/ml,全县由于轮虫造成浮头死亡的虾池3740亩,其中全部死亡的虾池740亩,部分死亡的虾池3000亩,其余的20000余亩的虾池正在大批浮头。 为了解决轮虫对养虾的危害,利用硫酸铜,杀死虾池中的轮虫和原生动物,取得较好的效果,现报告如下:     

轮虫休眠卵研究进展

综述了近年来国内外对轮虫休眠卵的研竞概况。重点介绍了轮虫休眠卵的形态、影响轮虫休眠卵形成的因素、轮虫休眠卵的萌发形式及影响其萌发的因素、自然水体中轮虫休眠卵的研究概况。     

营养强化的轮虫在鲻鱼育苗中的效用

经连续2a采用小球藻轮虫、酵母轮虫和乌贼肝油＋小球藻进行营养强化的轮虫培育鲻鱼苗，直观比较鱼苗的存活率和生长发育情况。结果表明：以乌贼肝油营养强化轮虫为饵的鱼苗，经35d的培育存活率最高，为25％－61％；以小球藻轮虫为饵的鱼苗次之，存活率为13％－24％；而以酵母轮虫为饵鱼苗的存活率是最低的，仅培育10－15d，仔鱼就全部死亡；以乌贼肝油营养强化轮虫为饵的鱼苗生长速度最快，个体大小整齐、肥满，变态时间比小球藻轮虫组提早1－2d，最长达3－4d。