口蹄疫病毒VP1植物表达载体的构建

P1 T重组质粒上含有口蹄疫病毒 (FMDV)GD10分离株的p1cDNA片段 ,以此为模板 ,用PCR方法扩增其中的VP1基因 ,获得大小约 6 40bp的片段。该片段用BglⅡ和BstEⅡ酶切消化后克隆至表达载体 pCAMBIA130 5 .2 ,转化EcoliTOP10感受态细胞。重组质粒经PCR、酶切及序列分析 ,证实VP1基因处于CaMV35S启动子控制 ,且读码框正确     

Endogenous viral elements in algal genomes

Endogenous viral elements （EVEs） are host-genomic fragments originated from viral genomes. They have been found universally in animal and plant genomes. Here we carried out a systematic screening and analy-sis of EVEs in algal genomes and found that EVEs commonly exist in algal genomes. We classified the EVE fragments into three categories according to the length of EVE fragments. Due to the probability of sequence similarity by chance, we ignored the potential function of medium-length EVE fragments. However, long-length EVE fragments probably had capability to encode protein domains or even entire proteins, and some short-length EVE fragments had high similarity with host＇s siRNA sequences and possibly served functions of small RNAs. Therefore, short and long EVE fragments might provide regulomic and proteomic novelty to the host＇s metabolism and adaptation. We also found several EVE fragments shared by more than 3 algal genomes. By phylogenetic analysis of the shared EVEs and their corresponding species, we found that the integration of viral fragments into host genomes was an ancient event, possibly before the divergence of Chlorophytes and Ochrophytes. Our findings show that there is a frequent genetic flow from viruses to algal genomes. Moreover, study on algal EVEs shed light on the virus-host interaction in large timescale and could also help us understand the balance of marine ecosystems.     

White spot syndrome virus inactivation study by using gamma irradiation

The present study was conducted to investigate the effect of gamma irradiation on white spot syndrome virus(WSSV). White spot syndrome virus is a pathogen of major economic importance in cultured penaeid shrimp industries. White spot disease can cause mortalities reaching 100% within 3–10 days of gross signs appearing. During the period of culture, immunostimulant agents and vaccines may provide potential methods to protect shrimps from opportunistic and pathogenic microrganisms. In this study, firstly, WSSV was isolated from infected shrimp and then multiplied in crayfish. WSSV was purified from the infected crayfish haemolymph by sucrose gradient and confirmed by transmission electron microscopy. In vivo virus titration was performed in shrimp, Penaeus semisulcatus. The LD50 of live virus stock was calculated 10 5.4 /mL. Shrimp post-larvae(1–2 g) were treated with gamma-irradiated(different doses) WSSV(10 0 to 10-4 dilutions) for a period of 10 days. The dose/survival curve for irradiated and un-irradiated WSSV was drawn; the optimum dose range for inactivation of WSSV and unaltered antigenicity was obtained 14–15 kGy. This preliminary information suggests that shrimp appear to benefit from treatment with gammairradiated WSSV especially at 14–15 KGy.     

盐度变化对携带白斑综合症病毒(WSSV)的凡纳滨对虾(Litopenaeus vannamei)的影响

研究盐度渐变和突变对WSSV在凡纳滨对虾(Litopenaeus vannamei)体内增殖的影响。结果表明,盐度由起始盐度(23±1)往高盐度(32±1)和低盐度(14±1)突变在整个实验中对凡纳滨对虾的累积死亡率和对虾体内病毒增殖的影响显著(P0.05),先感染WSSV实验,高盐度、起始盐度、低盐度累积死亡率分别为(61.1±10.7)%、(48.9±1.9)%、(63.3±12.0)%,病毒含量分别为6.0×107、3.7×106、5.3×107 copy/g;后感染WSSV实验,分别为(75.6±3.8)%、(52.2±13.4)%、(63.3±3.3)%;病毒含量分别为5.7×107、2.3×106、2.8×107 copy/g。盐度渐变实验各组至24h对虾死亡率低于18.9%,48h出现死亡高峰,72—96h存在显著差异(P0.05),先感染WSSV实验,高盐度、起始盐度、低盐度最大死亡率分别为(75.6±5.1)%、(38.9±5.1)%、(69.3±6.9)%,病毒含量分别为7.6×107、5.3×106、2.4×106copy/g;后感染WSSV实验分别为(46.7±10)%、(45.6±18.9)%、(74.4±13.9)%;病毒含量分别为5.1×106、4.8×105、1.0×107 copy/g。因此盐度变化会影响对虾的抗病能力,可造成对虾体内WSSV快速增殖;对携带WSSV对虾,盐度变化会大大提高WSSV从潜伏感染转为急性感染的可能,盐度是引起WSSV从潜伏感染转为急性感染的关键影响因子之一。     

重铬酸钾容量法测定铁中SnCl2加入超量的处理方法

重铬酸钾容量法测铁中,二氯化锡超量产生黑色汞沉淀,严重影响测定。利用空气氧化作用,无须添加任何试剂,在8-12min内解除汞的影响,较“弃之重来”的传统做法省时省力,用于铁矿样测定获满意结果,     

用ADM软件防止计算机病毒

用ＡＤＭ软件防止计算机病毒陈利群，章驾宇（成都理工学院计算机工程系）为了防止病毒的侵入，目前对磁盘管理常采用以下方法：１．封锁软驱，直接在硬盘上读、写。２．利用ＤＯＳ命令改变文件的属性，使系统文件为只读文件。防止人为破坏。３．用ＡＤＭ对硬盘贴上“标签...     

骨干网络安全防护技术探讨

目前网络的开放性和共享性在方便人们使用的同时,也更容易受到攻击,网络安全问题越来越引起人们的关注。文章通过对现有中国气象局骨干网络安全防护系统的简要分析,结合当今主流的网络安全防护理论和相关软硬件技术,阐述了在骨干网络中建立集入侵检测系统、安全漏洞扫描、防火墙系统和防病毒系统于一体的多层次、全方位的网络安全防护体系的必要性,并对骨干网络安全防护系统的改进方向提出了建议。     

网络时代的计算机病毒特征与防治

介绍了网络时代常见的计算机病毒特征及其防治方法.     

EPSONGT-10000+扫描仪一次故障排除

故障：打开EPSON GT-10000 自带图像扫描程序，点击或调用扫描程序后计算机提示“扫描仪未能找到或扫描仪不能打开”。反复调用及重启计算机调用，故障现象依然。检查：检查扫描仪各项硬件设置及数据线连接，SCSI接口、SCSIID号设置开关、关闭SCSI设备相互通信的外置终结器开关，启动扫描仪，观察启动过程中的各种反应属正常，排除了扫描仪本身故障的可能性；用杀毒工具病毒清除后故障未排除；将扫描应用程序及硬件驱动程序卸载后重装，故障仍未排除。     

广西龙滩电站麻村砂石系统溜井导孔钻探施工工艺

广西龙滩电站订村砂石系统溜井导孔钻探存在对孔斜和岩心采取率要求高、施工条件极差、工期要求紧的施工难点，通过采取有效技术措施，取得良好效果。介绍了该溜井导孔的防斜、取心、扩孔等技术措施。