浮萍发酵饲料养殖巴西鲷的试验

本试验应用纤维素酶对纤维素的降解，可以提高鱼类对粗纤维的利用率，促进养分的良好消化吸收的机理，将浮萍作为辅料与"菜籽饼"等饲料配比发酵养殖巴西鲷，适应其杂食性偏植物性的需要，主养巴西鲷越冬种396尾.经过144d养殖，产成鱼223.7kg，投入与产出比为13.18，取得了较好的经济效益.     

生物制氢：Ⅰ．理论研究进展

对生物制氢的理论研究进行了评论性的回顾。讨论了光合成生物制氢系统、光分解生物制氢系统、水气交换反应生物制氢系统、光合-发酵杂交生物制氢系统和厌氧发酵生物制氢系统、离体氢酶生物制氢系统等 6个生物制氢系统。乙醇型发酵生物制氢理论（双碳发酵产氢学说或理论）是生物制氢理论的新发展。     

未熟有机质发酵后

采用对未熟有机质富集培养本源菌再进行类发酵(Eh>-300mV)的模拟实验方法,重点研究了发酵样品中某些生物标志化合物的变化,探讨了在低演化阶段,由于细菌对有机质的参与和改造,液态烃类形成的地球化学特征。样品发酵后,烃类,尤其是低相对分子质量烃类含量增加,CPI和OEP值接近或略高于1。族组成中,沥青质含量减少,烃类和非烃增加。饱和烃中,C-13位上带有正烷基侧链的三环萜烷出现;高含量的伽马蜡烷被检出;生物构型藿类化合物向地质构型藿烷转化,C-22位上立体异构化平衡参数明显增大,接近或达到异构化终点值;单芳甾类烃与三芳甾类烃共存;甾体化合物含量的总体趋势是相对贫乏,平衡参数较低。     

闲话马奶酒和普通奶酒

近年来,人们介绍草原佳酿马奶酒的文章多了起来。这自然是好事。但有不少人的文章没分清马奶酒和普通奶酒之间的区别。其实两者的制作方法具有本质上的不同,马奶酒是发酵饮料,普通奶酒是蒸馏酒。     

海洋细菌LP621 右旋糖苷酶分批发酵动力学研究

对海洋细菌Pseudoalteromonas tetraodonis LP621右旋糖苷酶进行了50L发酵罐分批发酵的代谢特性的研究.结果表明LP621右旋糖苷酶合成和菌体生长呈部分生长偶联型.据分批发酵试验结果采用Logistic方程、Luedeking-Piret方程建立了描述菌株生长、产酶以及葡萄糖底物消耗分批发酵动力学模型.动力学模型计算值结果与实验值拟合良好,较好反映了菌株右旋糖苷酶分批发酵过程的动力学特征.     

Biological hydrogen production from organic wastewater with a novel strain B49 isolated from anaerobic sewage sludge

Water pollution of river basin has increased with increasing discharge of sewage and the water quality has deteriorated due to the discharge of organic wastewater. Pretreatment of organic wastewater before discharging is of significance for the protection of water resources. The anaerobic digestion of organic substrates has been a well-developed biological treatment method for wastewater and wastes. Especially, the biohydrogen production using organic wastewater can produce an energy product and simultaneously reduce the pollution intensity of the wastes. Microorganisms with high hydrogen-producing capacity perform an important function in biohydrogen production. In this paper, we investigated the zymolytic characteristics of a novel strain of bacteria B49 isolated from anaerobic activated sludge using waste liquid from a sugarhouse as substrate. The effect of yeast extract on microbial conversion of waste liquid from the sugarhouse was investigated. Different carbohydrates （such as glucose, sucrose, trisaccharide, molasses, etc.） in the waste liquid serve as major energy sources for B49＇s cell growth. The hydrogen yield of 2410 mL-H2/L-culture was obtained using glucose as substrate. When the waste liquid from the sugarhouse （molasses） was used as substrate, maximum hydrogen yield of 2460 mL-H2/L-culture was obtained at 2% of molasses.     

瑞丰生化公司一科研成果获江西省科技进步二等奖

在6月19日召开的江西省科学技术大会上，瑞丰生化有限责任公司张文宣、张金儿、赵南、朱江萍、刘义雄、黄学荃、宋友礼等人研究的《赤霉素产生菌高产菌株原生质体诱变筛选及发酵工艺的研究》科研成果，喜获江西省人民政府颁发的科学技术进步二等奖。在此之前的专家评审认为，该成果居于国内同类研究的领先水平。     

海洋放线菌Y18的分子鉴定、生物活性及其抗菌物质发酵条件优化

从北冰洋楚科奇海海洋沉积物中分离到一株放线菌Y18,其良好生长需要海水。经16S rRNA基因序列分析确定该菌株属于拟诺卡氏菌属(Nocardiopsis),次级代谢产物合成酶基因及抗菌活性筛选发现,它含有聚酮类Ⅱ型(PKSⅡ)和非核糖体含硫多肽类(non ribosome-bound sulfur-containing peptides,NRPS)化合物合成酶基因,并且对枯草芽孢杆菌和白色念珠菌有抑制作用。以枯草芽孢杆菌为指示菌对Y18的发酵条件进行了初步优化,适合的发酵条件为:发酵培养基(蛋白胨2 g,酪素水解物2 g,酵母膏2 g,葡萄糖1 g,海水1 L),种子液接种量4%,发酵温度28℃,发酵培养基初始pH值8.0,培养时间6天,1 000 mL三角瓶装液量20%。并对Y18发酵液稳定性进行了测定,结果显示:发酵液对酸碱、热的稳定性都较强,4℃下贮藏发酵液的抗菌活性保留时间较长。     

Optimization of fermentation conditions for 1,3-propanediol production by marine Klebsiella pneumonia HSL4 using response surface methodology

The industrially important organic compound 1,3-propanediol(1,3-PDO) is mainly used as a building block for the production of various polymers. In the present study, response surface methodology protocol was followed to determine and optimize fermentation conditions for the maximum production of 1,3-PDO using marine-derived Klebsiella pneumoniae HSL4. Four nutritional supplements together with three independent culture conditions were optimized as follows: 29.3 g/L glycerol, 8.0 g/L K 2 HPO 4, 7.6 g/L(NH 4) 2 SO 4, 3.0 g/L KH 2 PO 4, pH 7.1, cultivation at 35°C for 12 h. Under the optimal conditions, a maximum 1,3-PDO concentration of 14.5 g/L, a productivity of 1.21 g/(L·h) and a conversion of glycerol of 0.49 g/g were obtained. In comparison with the control conditions, fermentation under the optimized conditions achieved an increase of 38.8% in 1,3-PDO concentration, 39.0% in productivity and 25.7% in glycerol conversion in flask. This enhancement trend was further confirmed when the fermentation was conducted in a 5-L fermentor. The optimized fermentation conditions could be an important basis for developing lowcost, large-scale methods for industrial production of 1,3-PDO in the future.     

绿潮藻浒苔微生物高效降解及生物乙醇制备

从绿潮藻暴发海域的底泥和腐烂浒苔(Ulva prolifera)中,筛选出一株可高效降解浒苔纤维素的微生物菌株(F菌株),通过刚果红染色实验以及真菌的ITS分析,鉴定为曲霉属真菌。以浒苔为诱导培养基制备F菌株的粗酶液,测得其滤纸纤维素酶活为34.79 U/m L。在发酵实验中,当酶解条件为6%的粗酶液添加量、反应温度为38℃、反应时间为60 h、pH为6.8时,浒苔纤维素降解效果最好,酶解液发酵乙醇产量最高达到28.98 g/L。