虾夷扇贝TET基因在配子发生和早期发育中的表达模式

DNA甲基化参与调节动物配子发生和胚胎发育过程,TET基因负责DNA主动去甲基化,在基因印迹去除和细胞全能性获得中起重要作用。为了解海洋贝类配子发生和胚胎发育过程中DNA去甲基化如何发生,本研究以虾夷扇贝为研究对象,鉴定了其TET基因,并分析了该基因在性腺和胚胎幼虫发育中的表达变化。结果表明,虾夷扇贝基因组中含有1个TET基因(PyTET),该基因长39127 bp,包含10个外显子,编码1592个氨基酸,其蛋白具有完整的2OGFeDO超家族的加氧酶结构域。在性腺发育过程中,PyTET基因表达峰值出现在休止期精巢和增殖期卵巢,原位杂交结果显示其在精原细胞、精母细胞中均有表达,在卵母细胞中的表达明显高于卵原细胞;在早期发育过程中,其峰值出现在囊胚期。以上结果提示虾夷扇贝配子发生和胚胎发育过程中均发生了DNA主动去甲基化,这将有助于系统了解表观调控在贝类发育中的作用。     

回忆二位革命家在唐山地震时的决策

1时任北京卫戌区司令吴忠唐山地震发生时,北京卫戌区司令吴忠正在密云水库度假。我当时在密云水库地震台工作,负责测震观测。1976年7月28日凌晨3时42分,一场毁灭性的大地震降临在河北省冀东地区,北京的大地也发生了剧烈的振动。强烈地震过后一个多小时,大约5点多钟,天逐渐在亮起来,已能看清路边的草木,地震台领导找到我说“你快随这位解放军去水库管理处,首长要了解情况”。我当时只穿了背心、短裤,脚上是拖鞋,强烈的振动已顾不上礼节,我就跟着那位战士来到了密云水库接待大厅。大厅内只有吴忠司令员和身边的一位警卫员,大厅此时已成了卫戌…     

怀化市国土资源局科学利用国土资源促进地方经济发展——湖南省怀化市国土资源局探索"服务"新模式

连续6年实现全市耕地占补平衡 连续4年被评为全省矿产开发秩序先进单位 经营性土地使用权和矿业权出让100%实行招标、拍卖、挂牌出让 杜绝了新征用地因补偿安置未到位引起的上访 没有发生一起因地质灾害造成的群死群伤事件 2007年,怀化市国土资源局立足本地资源优势,坚持合理开发,科学利用,有力地促进了本地经济的发展.我们的思路是:上服务好国家、下服务好本地;对得起当今,还要对得起子孙.     

浅谈村民违法建房现象的成因及对策

近年来,农村违法建房现象时有发生,有些地方到了"村村点火,组组冒烟"的程度。而承担执法责任的国土、规划、城管等部门更是疲于奔命,但收效甚微。作为一名基层国土资源管理的工作人员,笔者认为当前村民违法建房有如下几个特点:     

海洋微藻间竞争研究进展

微藻是海洋生态系统中最主要的初级生产者,也是引发赤潮的主要生物[1-2].微藻之间的竞争作用是海洋生态系统的重要组成部分,对竞争机理的探讨,可进一步解释群落结构演替和赤潮发生原因,为赤潮治理提供新的思路,对海洋生态系统的修复和保护具有重要意义,竞争机理在微藻开发利用上的应用,将取得重大的经济效益和社会效益[3].     

Phylogenetic analysis of Pectinidae(Bivalvia) based on the ribosomal DNA internal transcribed spacer region

The ribosomal DNA internal transcribed spacer (ITS) region is a useful genomic region for understanding evolutionary and genetic relationships. In the current study, the molecular phylogenetic analysis of Pectinidae (Mollusca: Bivalvia) was performed using the nucleotide sequences of the nuclear ITS region in nine species of this family. The sequences were obtained from the scallop species Argopecten irradians, Mizuhopecten yessoensis, Amusium pleuronectes and Mimachlamys nobilis, and compared with the published sequences of Aequipecten opercularis, Chlamys farreri, C. distorta, M. varia, Pecten maximus, and an outgroup species Perna viridis. The molecular phylogenetic tree was constructed by the neighbor-joining and maximum parsimony methods. Phylogenetic analysis based on ITS1, ITS2, or their combination always yielded trees of similar topology. The results support the morphological classifications of bivalve and are nearly consistent with classification of two subfamilies (Chlamydinae and Pectininae) formulated by Waller. However, A. irradians, together with A. opercularis made up of genera Amusium, evidences that they may belong to the subfamily Pectinidae. The data are incompatible with the conclusion of Waller who placed them in Chlamydinae by morphological characteristics. These results provide new insights into the evolutionary relationships among scallop species and contribute to the improvement of existing classification systems.     

Seismically induced slope instabilities and the corresponding treatments: the case of a road in the Wenchuan earthquake hit region

On May 12, 2008, a magnitude 8.0 earthquake hit Wenchuan County, Sichuan Province resulted in great loss of life and properties. Besides, abundant landslides and slope failures were triggered in the most seriously hit areas and caused disastrous damages to infrastructures and public facilities. Moreover, abundant unstable slopes caused by the quake have the potential to cause damages for a considerable long period of time. The variety of these slopes and the corresponding treatments are connected with the topographical and geological conditions of the sites. It is decided to document and identify some of these major slope instabilities caused by the earthquake and their treatments. The paper shows the condition of a road in Dujiangyan through in situ explorations. The case history showed significant implications to the reconstruction of the quake-hit regions and future disaster prevention and management works.     

对虾传染性皮下及造血组织坏死病毒衣壳蛋白基因的克隆表达及抗体制备

本文根据GenBank登录的对虾传染性皮下及造血组织坏死病毒(IHHNV)的全基因组序列(NC-002190),设计出主要结构蛋白基因的相应引物,从厦门发病的对虾中,提取DNA,以此为模板,利用PCR扩增目的基因.再将目的基因分别与pGEX-6p1载体和pET-28a载体连接,构建重组表达载体pGEX-Ⅳ、pET-Ⅳ,重组子经酶切和测序鉴定后导入表达型大肠杆菌BL21,IPTG诱导表达,SDS-PAGE检测,大小约为62KDa和41KDa,与预测大小一致.将pET-Ⅳ表达重组蛋白经纯化,免疫小鼠,Western免疫印迹反应结果表明其抗体均可与pGEX-Ⅳ和pET-Ⅳ表达的蛋白发生特异性反应.本研究的结果为对虾IHHNV的快速检测及其免疫提供了研究基础.     

相手蟹属两种蟹类线粒体16SrRNA基因序列的比较

测定了相手蟹属（Sesarma）红螯相手蟹（S．haematocheir）和褶痕相手蟹（S．plicata）线粒体16SrRNA基因部分片段的序列,二者的序列长度相同,均为533bp,且A、T、G、C的含量相似,分别为198bp（37．1％）,206bp（38．6％）,84bp（15．8％）,45bp（8．4％）和200bp（37．5％）,205bp（38．5％）,81bp（15．2％）,47bp（8．8％）;二者的序列有49处差异,其中21个位点为转换、22个位点为颠换和6个缺失／插入位点。进一步对20种相手蟹属蟹类的长度为361bp的16SrRNA基因同源序列进行分析,发现AT的含量为78．6％～82．9％,明显高于GC的含量,且存有91个变异位点。从NJ树和遗传距离来看,在分布于中国的3种相手蟹中,无齿相手型（S．dehaani）和红螯相手蟹的亲缘关系最近（d=0．0151）,而它们与褶痕相手蟹的亲缘关系则较远（d=0．0924／0．09231。分布于中国的相手蟹和分布于北美的相手蟹之间存在着较大的遗传距离（差异）,表明它们之间有着较远的亲缘关系,互为单系起源。