欧氏六线鱼Hexagrammos otakii Jordan & Starks性腺发育的周年变化研究

根据对青岛附近水域欧氏六线鱼（已达性成熟年龄）性腺周年宏观和组织学观察，性腺发育可分为：①重复发育Ⅱ期；②开始成熟期；③接近成熟期；④临产期或产卵期；⑤产后期。按性腺指数变化并结合性腺组织切片确定，欧氏六线鱼性腺发育在青岛海区一年一个周期，繁殖期在１０月下旬至１２月，繁殖盛期是１１月下旬至１２月中旬。     

The development of Nemurella pictetii Klapálek (Plecoptera: Nemouridae) in two springstreams in central Europe

From April 1997 to June 1998 Nemurella pictetii populations were regularly sampled in two springstreams at 220 and 850 m a.s.l., respectively, in Hesse (Germany), at approximately 51°N. Random samples of larvae were taken at three week intervals during the vegetation period, and once a month during winter. Sex, instar, body length, head capsule width and wing pad length of all larvae were recorded. Temperatures were recorded every hour, temporal patterns of temperature agreed closely between sites. Mean winter lows were 3.9 °C at both sites, the mean summer high was 11.9 °C at the lower site, as opposed to 9.6 °C at the mountain site.At both sites, adult emergence started in May. At the mountain site, recruitment started in late July and continued into autumn. There was cohort splitting in the young generation. Some individuals grew rapidly until October–November, but last instar larvae first appeared in March the next year. 1600 degree-days above 0 °C were accumulated during complete development. At the lower site, recruitment began in early July, and cohort splitting also occurred. Fast growing summer recruits emerged as adults in late August, having accumulated only 700 degree-days (above 0 °C). Their offspring hatched in November-December and emerged the next spring, having accumulated also only 700 degree-days. However, only part of the population was bivoltine. Many of the summer recruits grew more slowly and accumulated close to 1900 degree days until they emerged the next spring, together with the offspring of their own fast-growing siblings. Dependence of growth rate on temperature could not be estimated and appears to vary with daylength. For example, 3–6 °C support growth and development provided daylength exceeds 10 hrs of light, or is rising.At both sites and in all cohorts individuals emerging earliest were larger than later emerging ones. The size decline is significantly correlated with number of days after the winter solstice. For the first time it is shown that the decline does not occur shortly before adult emergence but actually takes place several instars before the last. Size differences are then carried on, and amplified, during subsequent molts, until adulthood. The literature presently relates seasonal size declines of insects to high or rising temperatures experienced by larvae approaching adulthood. Our data show that, at least in Nemurella, this explanation fails. On average, females were distinctly larger than males. Differences in mean last instar size were noticed also between sites and years. They remain presently unexplained. The mean sex ratio in both populations was close to 1:1.     

Discourse,representation and urban planning: How a critical approach to discourse helps reveal the spatial re-ordering of street sex work1

This paper shows how a critical approach to discourse sheds light on processes of spatial re-orderings. It uses a case study of urban planning in an area of street sex work to explore the ways in which various representations of prostitution can be used to inform planning decisions. Representations of sex worker identity also expose complex spatial and social geographies and evolving processes of marginalisation and exclusion.     

基于性别差异的城市旅游电子地图的设计

城市旅游电子地图是人们出行旅游必备的信息指南。为了设计满足不同性别的旅游用户的需求的城市旅游电子地图,本文分析了基于性别差异用户的认知特点,以及性别差异对旅游电子地图编绘的影响。重点提出了基于性别差异的城市旅游电子地图的内容设计、符号设计和语音功能设计。最后,本文探讨了城市旅游电子地图的编制原则,主要包括内容设计原则、界面设计原则和符号设计原则。基于用户性别进行地图设计的研究思想可以更好地为广大用户提供更加全面翔实的旅游信息和服务。     

黄海南部小黄鱼洄游分布及种群结构特征

Monthly fishery survey data of the small yellow croaker Larimichthys polyactis in the southern Yellow Sea from2003 and 2013 were employed to evaluate the variation in the resource distribution and biological characteristics(especially body length and sex ratio) in the population on a decadal scale.The results indicated that the small yellow croaker migrated from the Shawai fishing ground to the Dasha fishing ground in spring and was mainly distributed in the central and western parts of the Dasha fishing ground in April and May.Larimichthys polyactis in the Dasha fishing ground migrated eastward to offshore wintering grounds in autumn and reached the central Dasha fishing ground in October and November.The small yellow croaker entered the western waters of the Shawai fishing ground in winter.A large number of age 0+ fish occurred in the Shawai and Jiangwai fishing grounds in October of 2003 and 2013.The body lengths of the spawning stock and wintering stock in 2013 were larger than those in 2003,and the monthly sex ratios(female to male) were significantly less than 1 in both years.The monthly distribution of this fish in the southern Yellow Sea was consistent with a previous finding that "the stock migrated between the wintering grounds in the west of Jeju Island and the Lüsi spawning grounds" but tended to move more northward,with the spawning grounds extending outward.In the past decade,body length variation experienced a decline after an increase,rather than a steady decrease.The sex ratio in the single-stick stow net showed a tendency to increase over the decade,but was either less than or more than 1 depending on the fishing gear;therefore,further studies should be conducted to determine the sex structure.     

Primary molecular basis of androgenic gland endocrine sex regulation revealed by transcriptome analysis in Eriocheir sinensis

In crustaceans, the male sexual dif ferentiation and maintenance are specially regulated by androgenic gland(AG). However, little is known about the genes involved in the regulation process.RNA-Seq was performed on AG with ejaculatory duct(AG_ED) and ejaculatory duct(ED) as control in Eriocheir sinensis, one of the most important economic and ?shery crabs with typically sex dimorphism. A total of 925 unigenes were identi?ed as dif ferentially expressed genes(DEGs) and the expression of nine genes randomly selected was con?rmed by qRT-PCR. 667 unigenes were up-regulated in AG_ED, being supposed to be AG preferential genes. Among them, the full length of i nsulin-like androgenic gland factor( IAG) cDNA named as Es-IAG was obtained as a logo gene of AG, which together with the genes i nsulin-like receptor( INR), and s ingle insulin binding domain protein( SIBD), might constitute the sex regulation pathway. Several sex related genes were identi?ed, and their function will have to be investigated. Also,the identi?cation of j uvenile hormone epoxide hydrolase 1( JHEH1), ecdysteroid 22-hydroxylase( DIB) and e cdysone receptor( ECR) preliminarily clari?ed the molecular regulation mechanism of eyestalk-AG-testis axis, which plays important roles in molting and reproduction. The results will enhance our understanding for the molecular basis of the AG involved in male sex regulation in crabs.     

凡纳滨对虾性别分化的初步研究

通过组织学连续切片与扫描电镜观察,研究凡纳滨对虾的性别分化。结果显示:凡纳滨对虾外部形态的性别分化最早发生在仔虾后第37天,此时在扫描电镜下可观察到雄性附肢的芽基,仔虾后第45天,光镜下可观察到交接器;仔虾后第24天出现原始生殖细胞(PGCS),位于背大动脉的两侧及围心腔膜的腹面,细胞个体及细胞核明显比周围的细胞大,具有强嗜碱性,细胞核圆形或椭圆形,核内染色质清晰可见,随着生殖腺的发育,在第43天出现生殖管,第55天生殖腺可区分为精巢和卵巢。     

Reproductive Biology of Yeilowfin Tuna T. albacares in the West-Central Indian Ocean

Survey of yellowfin tuna in the west-central Indian Ocean was conducted on board of Chinese Iongliners during 2003, 2004 and 2005, which is a part of Chinese Tuna Fishery Scientific Observer Program (CTFSOP). The reproductive biology has been investigated. A total of 1023 samples are collected including 417 ovaries and 606 testes. Spawning activities of yellowfin tuna have been studied for both male and female from January to June. The data showed that the average monthly sex ratio is 0.59, and the minimum length at sexual maturity is 101 cm for female and 110 cm for male respectively. Length at 50% sexual maturity is esti- mated at 113.77 cm for female and 120.20 cm for male, whereas maturation rate is 0.066 cm-1 for female and 0.091 cm-1 for male. Sex ratio by length class indicates that the proportion of male is higher than female's along with size increasing; for instance, in the group of the body length longer than 145 cm, some females have their body length from 145 to 160 cm and males have their body length at 160 cm and even longer. Statistically, yellowfin tuna has a significant seasonal reproduction.