菲律宾蛤仔(Ruditapes philippinarum)蛋白聚糖的分离提取及其抗肿瘤活性的初步研究

采用凝胶过滤层析、SDS-PAGE电泳、Bradford法和苯酚-硫酸法等生物化学方法对菲律宾蛤仔中的蛋白聚糖(PG1和PG2)进行分离纯化,研究其理化性质。采用MTT方法研究蛋白聚糖对人肝癌细胞SMMC-7721和人正常肝细胞HL-7702生长的影响。通过小鼠体内实验研究蛋白聚糖的抗肿瘤活性和对荷瘤小鼠免疫功能的影响。结果表明,PG1分子量大约为20kDa,多糖含量为70.09%,蛋白质含量为15.4%。PG2的多糖含量为82.53%,蛋白质含量为7.26%。PG1在200μg/ml浓度时对SMMC-7721的生长抑制率为73.30%,随浓度的升高,抑制率并未发生明显改变。PG2对SMMC-7721的生长抑制作用呈剂量依赖性,在500μg/ml的浓度下,其抑制效果与PG1的抑制效果相当。在同样浓度下,PG1对人正常肝细胞的生长和功能没有明显影响。PG1呈剂量-效应关系明显增加荷瘤小鼠脾指数,促进荷瘤小鼠脾淋巴细胞转化,且没有降低体重及其他毒副作用。PG1对小鼠S180肉瘤的抑制作用可能是通过直接杀伤肿瘤细胞的细胞毒作用和提高小鼠细胞免疫水平来实现。     

重金属锌,铅对菲律宾蛤仔的急性毒性试验

本文研究了必需元素Ｚｎ及非必需元素Ｐｂ对菲律宾蛤仔的急性毒性作用，得到Ｚｎ对其的４８ｈＬＤ５０和９６ｈＬＤ５０分别为１４７．９１、１６．４０ｍｇ／ｄｍ＾３；Ｐｂ对其的４８ｈＬＤ５０和９６ｈＬＤ５０分别为３１．６２、１４．２８ｍｇ／ｄｍ＾３。估算得到Ｚｎ、Ｐｂ对菲律宾蛤仔的安全浓度分别为０．８２、０．７１ｍｇ／ｄｍ＾３。     

菲律宾上游油气工业概况及其税费制度

2004年以来30多个国内外石油公司进入上游领域总合同数目达29个,合同覆盖面积扩大了15倍,巴拉望盆地西北地区是菲律宾最重要的油气区,菲律宾的石油工业的财税体制在亚太地区是最具吸引力的地区之一。菲律宾产量分成合同中（PSC）方式分成比例为60％：40％,其中准予扣减的项目包括过去成本的回收;操作、资金和利息成本的回收;深水激励;当地参与激励;矿区使用费;公司所得税;国家参与和加速折旧等。     

2004年全球重大气候事件概述

2004年全球气候持续偏暖。年初，暴雪席卷欧洲多国，美国受到罕见大雪袭击，南亚和墨西哥遭遇低温严寒。北半球夏秋季，西太平洋、大西洋热带风暴活动频繁。日本、菲律宾和美国受灾严重。年内，非洲、亚洲部分国家发生严重干旱；同时，南亚、南美洲及非洲多国暴雨致洪。年内，欧洲伊比利亚半岛、日本和澳大利亚遭遇高温热浪。     

菲律宾迪纳加特岛30#矿权区红土型镍矿床地质特征及成矿作用探讨

30~#矿权区位于菲律宾迪纳加特岛北部,区内发育有红土型镍矿床。本次勘查圈定4处矿(化)体,其中以Ⅰ、Ⅱ、Ⅲ矿(化)体的矿化范围较大,厚度变化系数为45%~50%(相对稳定),经济价值较高。红土型镍矿体平面上呈地毯状展布于地表,厚度受地形控制,富镍矿体分布于矿权区东部的5、6、11、12小区;剖面上从地表向深部矿体为铁质红土层、松散红土层及高岭土化蛇纹石化橄榄岩层。30~#矿权区红土镍矿矿床的形成经历了从普通矿到富矿两个阶段,红土镍矿石属于酸性矿石。     

Mineralogical and Geochemical Constraints on Arsenic Mobility in a Philippine Geothermal Field

Arsenic is usually associated with sulphide minerals formed in the geothermal environment. However, sulphide minerals are prone to dissolution after contact with meteoric water under surface oxidizing conditions. Secondary precipitates that form from the dissolution of the primary sulfides exert a greater influence on arsenic mobility in the geothermal environment. Fe-hydroxides have very good affinity with dissolved arsenate and are stable under most surface oxidizing conditions. Both amorphous silica directly precipitated from geothermal fluids and possibly a kaolinite alteration can host a small significant amount of arsenic. These silicates are also more stable under a wide range of pH and redox conditions.     

Mineral provinces and matter provenance of the surficial sediments in the western Philippine Sea: implication for modern sedimentation in West Pacific marginal basin

The characteristics and distribution patterns of detrital minerals (0.063~0.125 mm) in marine sediments provide a significant indicator for the identification of the origin of sediment.The detrital mineral composition of 219 surface sediment samples was analysed to identify the distribution of sediments within the western Philippine Sea. The area can be divided into three mineral provinces: (I) province east of the Philippine Trench, the detrital minerals in this province are mainly composed of calcareous or siliceous organisms, with the addition of volcanogenic minerals from an adjacent island arc; (II) middle mineral province, clastic minerals including feldspar, quartz and colorless volcanic glass, sourced from seamounts with intermediate-acid volcanic rock, or erupting intermediate-acid volcano; (III) province west of the Palau—Kyūshū Ridge, the matter provenance within this province is complex; the small quantity of feldspar and quartz may be sourced from seamounts or erupting volcano with intermediate-acid composition, with a component of volcanic scoria sourced from a volcano erupting on the Palau—Kyūshū Ridge. it is suggested that, (1) Biogenic debris of the study area is closely related to water depth, with the amount of biogenic debris controlled by carbonate lysocline. (2) Volcaniclastic matter derived from the adjacent island arc can be entrained by oceanic currents and transported towards the abyssal basin over a short distance. The weathering products of volcanic rocks of the submarine plateau (e.g.,Benham Plateau) and adjacent ridges provide an important source of detrital sedimentation, and the influence scope of them is constrained by the intensity of submarine weathering. (3) Terrigenous sediments from the continent of Asia and the adjacent Philippine island arc have little influence on the sedimentation of this study area, and the felsic mineral component is probably sourced from volcanic seamounts of intermediate-acid composition.     

Accumulation of petroleum hydrocarbons and heavy metals in clams （Ruditapes philippinarum） in Jiaozhou Bay, China

Accumulation and distributions of aliphatic and polyaromatic hydrocarbons (PAHs) and heavy metals were measured in tissues of the clam Ruditapes philippinarum collected from 5 sites in Jiaozhou Bay, Qingdao, China. The concentrations of total aliphatic hydrocarbon and PAHs ranged from 570 to 2 574 ng/gdw (gram dry weight) and from 276 to 939 ng/gdw, in the most and least polluted sites, respectively. The bio-accumulation of hydrocarbons and PAHs in the clams appeared to be selective. Aliphatic hydrocarbons were predominantly represented by short chain (<nC₂₃) n-alkanes, suggesting that petroleum hydrocarbons were likely the major contamination source. The selective uptake of 3 and 4 ring PAHs, such as naphthalene, fluorene, phenanthrene, fluoranthene and pyrene, by the clams was probably related to the physiological and bio-kinetic processes that were energetically favorable for uptake of compounds with fewer rings. Accumulation of the metals Cd, Cu, Zn, Pb, Cr, Hg, and As in the clam tissues also showed high variability, ranging from 0.043 to 87 μg/gdw. Among the 7 detected metals, Zn, Cd, Cu, and As had a particularly high potential of accumulation in R. philippinarum. In general, a positive correlation was found between the tissue concentrations and sediment concentrations of hydrocarbons and of some metals. Our study suggests that moderate contamination with polyaromatic hydrocarbons, and low to moderate contamination with metals, currently exists for clam R. philippinarum in Jiaozhou Bay, in comparison with other regional studies. A long-term monitoring program is certainly needed for assessment of the potential ecological influence and toxicity of these contaminants of R. philippinarum in Jiaozhou Bay.     

中国人的自信从何而来

某所大学请我去演讲,我问了大学生一个问题:作为中国人,你有自豪感吗?大家一致回答,有!我问:那到底是什么让你产生这种自豪感的呢?有人回答是长江、长城,也有回答是经济的高速发展、5000年的文明、文字,奥运、火箭升空……我紧接着又问:假如清晨醒来,你突然发现自己变成了一个菲律宾人,你还会为自己感到自豪吗?或者,你变成了一个卢旺达人,生活在非洲,经历过种族屠杀,经常处于饥饿状态,此时,你还能够