渤海大型底栖动物次级生产力的初步研究

1998年 9月和 1999年 4月分别对渤海 2 0个站进行了大型底栖动物两个航次的调查取样。整个研究海域大型底栖动物年次级生产力平均值为 6 .4 9g(AFDW) /(m2 · a) ,其中渤海海峡以东海域较高 ,为 12 .5 9g(AFDW) /(m2· a) ;渤海中部为 4 .4 6 g(AFDW) /(m2· a) ,渤海大型底栖动物年平均 P/B为 0 .82     

Theoretical aspects of cap-rock and fault seals for single- and two-phase hydrocarbon columns

Cap-rock seals can be divided genetically into those that fail by capillary leakage (membrane seals) and those whose capillary entry pressures are so high that seal failure preferentially occurs by fracturing and/or wedging open of faults (hydraulic seals). A given membrane seal can trap a larger oil column than gas column at shallow depths, but below a critical depth (interval), gas is more easily sealed than oil. This critical depth increases with lower API gravity, lower oil GOR and overpressured conditions (for the gas phase). These observations arise from a series of modelling studies of membrane sealing and can be conveniently represented using pressure/ depth (P/D) profiles through sealed hydrocarbon columns. P/D diagrams have been applied to the more complex situation of the membrane sealing of a gas cap underlain by an oil rim; at seal capacity, such a two-phase column will be always greater than if only oil or gas occurs below the seal.These conclusions contrast with those for hydraulic seals where the seal capacity to oil always exceeds that for gas. Moreover, a trapped two-phase column, at hydraulic seal capacity will be less than the maximum-allowed oil-only column, but more than the maximum gas-only column. Unlike membrane seals, hydraulic seal capacity should be directly related to cap-rock thickness, in addition to the magnitude of the minimum effective stress in the sealing layer and the degree of overpressure development in the sequence as a whole.Fault-related seals are effectively analogous to membrane cap-rocks which have been tilted to the angle of the fault plane. Consequently, all of the above conclusions derived for membrane cap-rocks apply to both sealing faults sensu stricto (fault plane itself seals) and juxtaposition faults (hydrocarbon trapped laterally against a juxtaposed sealing unit). The maximum-allowed two-phase column trapped by a sealing fault is greater than for equivalent oil-only and gas-only columns, but less than that predicted for a horizontal membrane cap-rock under similar conditions. Where a two-phase column is present on both sides of a sealing fault (which is at two-phase seal capacity), a deeper oil/water contact (OWC) in one fault block is associated with a deeper gas/oil contact (GOC) compared with the adjacent fault block. If the fault seal is discontinuous in the gas leg, however, the deeper OWC is accompanied by a shallower GOC, whereas a break in the fault seal in the oil leg results in a common OWC in both fault blocks, even though separate GOC's exist. Schematic P/D profiles are provided for each of the above situations from which a series of fundamental equations governing single- and two-phase cap-rock and fault seal capacities can be derived. These relationships may have significant implications for exploration prospect appraisal exercises where more meaningful estimates of differential seal capacities can be made.The membrane sealing theory developed herein assumes that all reservoirs and seals are water-wet and no hydrodynamic flow exists. The conclusions on membrane seal capacity place constraints on the migration efficiency of gas along low-permeabiligy paths at depth where fracturing, wedging open of faults and/or diffusion process may be more important. Contrary to previous assertions, it is speculated that leakage of hydrocarbons through membrane seals occurs in distinct pulses such that the seal is at or near the theoretically calculated seal capacity, once this has been initially attained.Finally, the developed seal theory and P/D profile concepts are applied to a series of development geological problems including the effects of differential depletion, and degree of aquifer support, on sealing fault leakage, and the evaluation of barriers to vertical cross-flow using RFT profiles through depleted reservoirs. It is shown that imbibition processes and dynamic effects related to active cross-flow across such barriers often preclude quantitative analysis and solution of these problems for which simulation studies are usually required.     

长江口及其邻近海域大型底栖动物生物量、丰度和次级生产力的初步研究

为了解长江口及邻近海域大型底栖动物生物量、丰度和次级生产力的分布情况于2004年2月、5月、8月和11月共4个航次分别在长江口40个观测站采集大型底栖动物定量样品并利用Brey的经验公式对大型底栖动物栖息丰度、生物量、次级生产力和P/B值进行了研究计算.该调查海域共采到大型底栖动物202种,其中多毛类102种,软体动物51种,甲壳类27种,棘皮动物7种,其它动物15种.大型底栖动物年平均丰度为394.7 ind/m2;年平均生物量以去灰分干重计,为2.58 g(AFDW)/m2;年平均次级生产力以去灰分干重计,为3.52 g(AFDW)/(m2*a);P/B值平均为1.53.结果表明,长江口大型底栖动物次级生产力自长江入海口向东呈递增趋势.本文分析了长江口及其邻近海域大型底栖动物优势种的组成,主要生态类群的分布特征和次级生产力分布格局与生态环境的关系.通过比较,发现长江口大型底栖动物优势种发生了较大的变化;次级生产力高于东海而低于渤海和胶州湾;P/B值高于南黄海、胶州湾和渤海,也说明了长江口大型底栖动物群落中个体小、生活史短,代谢快的种类所占的比例高于以上海域.     

基于多元统计分析的渔山列岛潮间带软体动物功能群和次级生产力比较

渔山列岛于2008年成为国家级海洋生态保护区,为了解渔山列岛保护区内潮间带软体动物次级生产力的时空变化。于1982年和2010年的3个季节(春、夏、冬)在渔山列岛进行潮间带软体动物调查,采用多元统计分析软体动物时空差异,以Brey经验公式计算分析软体动物次级生产力(P值,P/B值)。结果表明,年际间物种食性结构发生了明显的改变,1982年潮间带软体动物对次级生产力贡献率最大的物种有单齿螺(Monodonta labio)、锈凹螺(Chlorostoma rustica),占软体动物总次级生产力的42.88%,2010年潮间带软体动物对次级生产力贡献率最大的物种有覆瓦小蛇螺(Serpulorbis imbricata)、条纹隔贻贝(Septifer virgatus),占软体动物总次级生产力的75.96%。1982年,年均栖息密度为2758ind/m2,年均生物量为2100.26g/m2,年均次级生产力为240.04g/(m2·a),年均P/B值为0.63a–1;2010年,年均栖息密度为699ind/m2,年均生物量为1101.85g/m2,年均次级生产力为94.82g/(m2·a),年均P/B值为0.55a–1。多因素方差分析表明软体动物次级生产力年际间差异显著(F=5.761,P0.05),季节间和潮位间差异都不显著(F=0.135,P0.05;F=2.076,P0.05),P/B的值较低,表明该海域软体动物世代更替速度较慢,2010年的P/B值(0.55a–1)低于1982年的P/B值(0.63a–1),表明近30年来群落结构受到影响。     

基准网平差初至波定位方法及应用

在滩浅海地震勘探中,由于海水深度和潮流的变化,在水面上按照预定位置沉放的检波器,在海底不可能放置在预定的位置。为此,利用基准网平差初至波定位方法对海底检波器位置进行了准确的二次定位,并对初至波二次定位和声波二次定位进行了比较分析。基准网平差初至波定位方法在KD-1滩浅海地区高精度地震勘探应用中取得了良好的效果。     

深圳湾北岸泥滩大型底栖动物次级生产力研究

根据2005年3、6、9月和12月在深圳湾北岸泥滩9个取样站采集的大型底栖动物4个季度的定量样品,运用Brey(1990)的经验公式进行了大型底栖动物栖息丰度、生物量、次级生产力和P/B值的研究计算.整个研究区域大型底栖动物年次级生产力平均值(去灰干重)为54.55g/(m2.a),其中观鸟屋附近泥滩(A断面)较高,为68.85g/(m2.a),凤塘河口附近泥滩(H断面)为50.62g/(m2.a)次之,沙嘴码头附近泥滩(F断面)较低,为44.18g/(m2.a),深圳湾北岸泥滩大型底栖动物年平均P/B为2.95.可见,大型底栖动物次级生产力越靠近深圳河河口越低,这与越靠近深圳河河口,大型底栖动物个体越小、生活史更短的结果是一致的.     

Phaeocystis globosa与Phaeocystis antarctica叶绿体psbA基因的比较

测定了2株球形棕囊藻Phaeocystis globosa P1、P2的psbA基因序列。发现得到的2个序列完全相同。以P2序列对比分析了P．globosa和P．antarctica的psbA基因在DNA序列、氮基酸序列和RNA二级结构上的差异性，发现2种棕囊藻psbA基因DNA序列和氨基酸序列非常保守，无插入／缺失，其核苷酸和氨基酸变异率分别为1．88％和1．13％。与核基因核苷酸的碱基替换不同，psbA基因核苷酸的碱基替换主要发生在密码子的第1位上。且不引起氨基酸的变化，引起氨基酸变化的碱基替换都发生在密码子的第2位和第3位上。在RNA二级结构上两序列的1～4茎环结构完全相同，表现出明显的棕囊藻属的特异性，其它结构区域差异较大。种间差异表现明显。由于psbA基因DNA序列和氨基酸序列非常保守，可能不适宜棕囊藻属的系统发育分析。但其RNA二级结构可能对于棕囊藻的分子分类有一定的参考价值。     

一株深海耐嗜压菌的细胞毒活性次生代谢产物研究

通过CCK8法检测分离自深海一株深海耐压菌Shewanella piezotolerans WP3次生代谢产物的细胞毒活性,研究了温度、压力、噬菌体对WP3次生代谢产物细胞毒活性的影响.发现WP3次生代谢产物具有良好细胞毒活性.低温因素可提高其细胞毒活性,在100μg/cm^3的含量下,代谢产物对肿瘤细胞Bel7402、CNE2、A549、HT1080细胞毒活性均有提高,其中对A549细胞毒活性提高了1.3倍.在最适温度（20℃）情况下,高压可降低其细胞毒活性.20 MPa培养条件下,代谢产物对4株肿瘤细胞均未表现出明显活性,而常压培养条件下对SW 480具有93.3%的抑制率,对HT1080抑制率表现出87.5%,对CNE2抑制率是79.8%.在低温情况下,压力因素对其细胞毒活性无影响,噬菌体对细胞毒活性影响不大.HPLC指纹图谱分析发现不同温度条件下,其次生代谢产物指纹图谱有变化,结合细胞毒活性结果分析,为后期分离鉴定细胞毒活性化合物提供指导.     

近地天体望远镜增加卡氏内氏焦点的可行性研究

近地天体望远镜是巡天望远镜, 具有短焦距、大视场、低空间分辨率的光学特点. 望远镜只有一个主焦点, 焦距1.8 m, 底片比例9um/'', 像斑几何能量集中度EE80 ≤2''(像斑环绕能量的80%,即80% encircled energy, 记为EE80), 有效视场直径为4.28-°(14.3deg2), 目前配10k times 10k的STA1600LN CCD (charge-coupled device) camera, 观测视场为9deg2. 通过光学系统设计, 在原光学系统上增加副镜及场改正镜, 获得了焦距9m的卡氏焦点和内氏焦点，底片比例43.6 um /"，在直径15''的可用视场内，像斑EE80≤0.5",为近地天体望远镜实现多终端观测提供了理论依据.     

近场动力学理论在区域地面沉降建模中的应用研究

地面沉降是由自然和人类活动共同作用导致的缓变性地质灾害,是区域可持续发展的重要影响因素。以往地面沉降在建模时需要地裂缝等不连续结构的位置、走向、深度等信息,在构建微分方程模拟时需假设模型连续,这对开展地面沉降-地裂缝-地面塌陷一体化模拟带来一定的局限性。于2000年出现的近场动力学理论提出以积分形式描述材料受力过程,方法具有无需先验知识与连续性假设的优点,在材料的疲劳、损伤、断裂等模拟研究中展现出良好的应用前景。本文综述了其在岩石水力压裂、滑坡、饱水岩土材料等领域的研究进展;提出了应用近场动力学进行区域地面沉降建模求解的一般方法;结合InSAR技术获取的沉降信息、三维地震频谱谐振勘探技术获取的地下结构与密度信息等,提出了模型的反演调参与优化方法。以北京东部梨园-台湖镇-张家湾镇区域为实验区,建立了其4 km×6 km范围、0.2 km深度的近场动力学地面沉降模型,以实验区地下水位月均下降速率为边界条件,对2007—2010年地面沉降过程进行模拟。模拟结果与实测值的平均绝对误差为18 mm,表明近场动力学在地面沉降建模中有较好的适用性。