Advanced characterization of marine dissolved organic matter by combining reversed-phase liquid chromatography and FT-ICR-MS

Marine dissolved organic matter (DOM) was separated by reversed-phase (RP) liquid chromatography method and analyzed with fluorescence/absorption detection and Fourier transform ion cyclotron mass spectrometry (FT-ICR-MS). The three key characteristics of the RP method are: (a) The C18-RP column chosen provides enhanced separation when the aqueous phase is 100% buffer-free water, and it does not degrade over time; (b) the water eluent adjusted to pH 7 significantly improves the resolution of water soluble compounds; (c) the initial flow maintained at low levels improves the separation of polar compounds. In samples, containing “fresh” DOM, specific peaks were detected, which were absent in “old” DOM samples. The combination with size exclusion chromatography (SEC) also demonstrated the relation between polarity and molecular size of DOM. FT-ICR-MS was applied to evaluate the quality of separation on a molecular scale demonstrating that physico-chemical characteristics of DOM can be related to molecular formulas. Sample extracts were separated into 4 preparative fractions, and a large suite of the identified molecular formulas only occurred in specific fractions. This is an important basis for the application of further analytical techniques in order to perform a more target-oriented analysis aiming at the determination of source and process biomarkers for DOM.     

Three-dimensional modeling of tidal circulation and mixing over the Java Sea

A combination of a three-dimensional hydrodynamic model and in-situ measurements provides the structures of barotropic tides, tidal circulation and their relationship with turbulent mixing in the Java Sea, which allow us to understand the impact of the tides on material distribution. The model retains high horizontal and vertical resolutions and is forced by the boundary conditions taken from a global model. The measurements are composed of the sea level at coastal stations and currents at moorings embedded in Seawatch buoys, in addition to hydrographic data. The simulated tidal elevations are in good agreement with the data for the K₁ and M₂ constituents. The K₁ tide clearly shows the lowest mode resonance in the Java Sea with intensification around the nodal point in the central region. The M₂ tide is secondary and propagates westward from the eastern open boundary, along with a counterclockwise amphidromic point in the western part. The K₁ tide produces a major component of tidal energy, which flows westward and dissipates through the node region near the Karimata Strait. Meanwhile, the M₂ tide dissipates in the entire Java Sea. However, the residual currents are mainly induced by the M₂ tide, which flows westward following the M₂ tidal wave propagation. The tidal mixing is mainly caused by K₁ tide which peaks at the central region and is consistent with the uniform temperature and salinity along the vertical dimension. This mixing is expected to play an important role in the vertical exchange of nutrients and control of biological productivity.     

ABUNDANCE AND PRODUCTION OF BRANCHIURA SOWERBYI (OLIGOCHAETA: TUBIFICIDAE) IN TWO TYPICAL SHALLOW LAKES (HUBEI, CHINA)

ＩＮＴＲＯＤＵＣＴＩＯＮＢｒａｎｃｈｉｕｒａｓｏｗｅｒｂｙｉ,ａｃｏｓｍｏｐｏｌｉｔａｎｔｕｂｉｆｉｃｉｄ,ｉｓａｐｒｅｄｏｍｉｎａｎｔｚｏｏｂｅｎｔｈｏｓｉｎｍａｎｙｓｈａｌｌｏｗｌａｋｅｓｉｎＣｈｉｎａ(Ｌｉａｎｇ,1979).Ｉｎ1984,ｔｈｅｐｒｏｄｕｃｔｉｏｎｒａｔｅｓｏｆｔｈｅｓｐｅｃｉｅｓｗｅｒｅｆｉｒｓｔｅｓｔｉｍａｔｅｄｉｎＤｏｎｇｈｕＬａｋｅ,Ｗｕｈａｎ(Ｌｉａｎｇ,1984).Ａｆｔｅｒｔｈａｔ,ｎｏｄａｔａｏｎｐｒｏｄｕｃｔｉｏｎｏｆｔｈｉｓｗｏｒｍｏｒｏｔｈｅｒｚｏｏｂｅｎｔｈｏｓｗｅｒｅｐ…     

海洋放线菌H2003代谢产物中环二肽成分及其抗肿瘤活性的初步研究

海洋来源放线菌H2003发酵产物的粗提物对小鼠乳腺癌细胞tsFT210具有明显的细胞毒活性。为了发现其抗肿瘤活性成分,采用活性跟踪的分离方法,经多次硅胶柱色谱、凝胶柱色谱及高效液相色谱分离,从菌株H2003发酵液中分离得到了7个环二肽化合物。经核磁共振光谱、质谱和旋光分析,其结构分别鉴定为cyclo(L-Leu-L-Pro)(1),cyclo(D-Val-L-Pro)(2),cyclo(Gly-L-Leu)(3),cyclo(L-Pro-L-Try)(4),cyclo(Gly-D-Phe)(5),cyclo(Gly-L-Pro)(6)和cyclo(L-Ala-L-Phe)(7)。其中化合物1通过SRB法测试表现出弱的抗肿瘤活性,系首次报道。     

高空西风急流对祁连山区降水的影响

利用NCEP/NCAR逐日每6 h再分析资料,对2002~2004年发生在祁连山区附近的20次降水过程进行了合成分析。发现在40°N200 hPa高空存在一支东西走向的高空西风急流;降水一般发生在高空西风急流轴的南侧,在高空西风急流轴的北侧有冷空气下沉,南侧有上升的暖湿气流,在高空急流轴的下方形成了次级环流;在高空急流轴出口区的右侧,存在强烈的辐散上升运动,而对应的低层存在强烈的辐合上升区,这种高低层辐散辐合的配置非常有利于强对流天气形成;这支高空西风急流轴的存在对祁连山区的降水有深远影响,而急流轴位置的变化势必影响到降水落区。因此,利用高空西风急流轴预报祁连山区降水及河西走廊地区降水具有很好的指导意义。     

7个月周期极移的非线性动力机制

从滞弹性阻尼形变摄动造成CW频率调制假设出发,对CW的共振激发模型加上了参数的时变调制,变成了参数共振模型。经正演计算发现,参数共振模型完全符合CW的实际,表明滞弹性阻尼形变摄动造成频率的3%调制,进一步使得CW振幅调制可达70%以上。这一参数共振模型是一个非线性动力系统,在非线性情况下,运动将发生分岔,即多解。     

Chiari病合并神经性关节病的影像表现

目的:分析Chiari病合并神经性关节病的影像表现。方法:回顾性分析8例Chiari病所致神经性关节病的影像学表现。结果:8例中全部为Chiari病合并脊髓空洞症,其中3例为Chiari病术后。17个关节病累及肩关节11例,肘关节6例。增生为主型7例,影像学表现为关节增生硬化及骨赘形成。吸收为主型10例,影像学表现为关节面不规则骨质碎裂、溶解、残端刀削样改变。两型均可伴骨髓水肿(其中5个部位累及骨干)、关节游离体及关节肿胀积液。X线能较好显示关节脱位、骨质增生硬化及吸收。16层CT结合后处理还可多角度观察到大部分游离体位置及数目。MRI能准确地诊断Chiari病合并的脊髓空洞、显示骨干水肿及软组织改变能力优于X线及CT,水成像序列显示关节积液内微小游离体的能力优于其他检查。结论:X线、CT与MRI检查手段相结合有助于Chiari病合并神经性关节病的影像学诊断。     

岩心核磁共振测量数据处理方法在北黄海东部坳陷中的应用

岩心核磁共振测井技术具有测量结果准确、对岩石结构无损害的优势,在油田勘探开发中应用越来越广泛。针对北黄海东部坳陷中、新生代有岩心核磁共振的资料进行解谱处理方法研究,确定了预处理、解谱和地层参数求取方法与流程,实现了岩心核磁共振资料解谱处理。根据最优解谱方法得到有效孔隙度、可动流体孔隙度、束缚水孔隙度和渗透率等地层参数。     

山东蓝宝石的宝石矿物学特征

对山东蓝宝石进行了系统的宝石矿物学研究,描述了山东蓝宝石的形态,大小,结构及包裹体特征,运用电子探针,粉晶Ｘ射线分析,顺磁共振光谱及化学全分析等测试手段,揭示了山东蓝宝石的成分,结构和物性之间的相互关系。     

结合混合源面波与三分量频率谐振的浅震勘探在城市地质结构精细划分中的应用

结合了混合源面波与三分量频率谐振的浅震勘探技术,综合了主动源面波探测精度高和被动源面波探测深度大的优点,同时增加了三分量频率谐振拟波阻抗比值这一参数进行综合推断解释,大大提高了探测的分辨率和准确性.采用该浅震勘探技术在成都天府国际生物城开展了浅层地质结构的精细化探测工作,快速获取了工作区60 m以浅地层结构的三维S波速度及三分量频率谐振拟波阻抗比值分布特征,利用波速与波阻抗的差异性,结合大量的工程钻孔资料标定,实现了对地层结构的精细划分,同时也证明了该浅震勘探技术在城市浅层地质结构精细化探测中的有效性.