碱消解-高效液相色谱-电感耦合等离子体质谱法测定生物样品中的甲基汞和乙基汞

建立了碱消解-高效液相色谱-电感耦合等离子体质谱联用系统测定生物样品中甲基汞(MeHg)与乙基汞(EtHg)的分析方法。为提高灵敏度,选用微流量的PFA雾化器,在优化的检测条件下,MeHg及EtHg检出限可达到0.036μg/L和0.03μg/L;线性范围达到4个数量级,两条工作曲线线性相关系数为1。对1.78μg/L MeHg、1.65μg/L EtHg的混合标准溶液重复测定7次,色谱峰面积的相对标准偏差(RSD)分别为1.79%和1.44%。对标准物质BCR 464(金枪鱼)的分析结果表明,测定值与标准值基本吻合,但略低于标准值;甲基汞和乙基汞的加标回收率分别为85.9%和84.5%。高效液相色谱与质谱联用技术的高灵敏度和低检出限能够满足生物样品中汞形态定量分析的要求。     

滑坡体三维地质建模与可视化分析

针对滑坡地质研究的自身特点,提出了面向滑坡地质体三维建模的NURBS-BRep混合数据结构和地质结构单元实体构造技术.通过对滑坡基础地质数据的预处理、滑坡数字地形和滑动面的三维建模、地质结构面的系统构造、地质结构体的生成和显示,形成了一套完整的滑坡三维地质模型的构建方法.将该方法应用于某水库滑坡,建立了相应的三维滑坡地质模型,并基于此模型进行了三维剖切分析、数字钻孔、等值线生成、滑块自动剖分、滑坡失稳可视化动态模拟和滑坡体方量精确计算等一系列实用的可视化分析,为滑坡稳定性的准确计算和客观评价提供了有力的支持.     

1998年夏、冬季南海水团分析

为了解南海水团的特征和分布 ,基于 1 998年夏季和冬季两个航次的实测资料 ,采用聚类分析、判别分析和模糊分析方法 ,对南海的水团进行了分析。结果表明 ,南海外海水可划分为 6个水团 ,即南海表层水团、南海次表层水团、南海次 中层混合水团、南海中层水团、南海深层水团和南海底盆水。越南附近夏季存在一个暖涡 ;1 998年夏季还可鉴别出黑潮表层水团和黑潮次表层水团 ,但在冬季观测期间无黑潮水越过 1 1 9.5°E经线进入南海 ;这些现象可能与厄尔尼诺现象有关联。夏季有苏禄海海水在 5 0— 75m层经由民都洛海峡侵入南海     

Numerical Experiment on Two-Dimensional Line Thermal

The time evolution of a two-dimensional line thermal-a turbulent flow produced by an initial element with signifi-cant buoyancy released in a large water body, is numerically studied with the two-equation k - e model for turbulence closure. The numerical results show that the thermal is characterized by a vortex pair flow and a kidney shaped concentra-tion structure with double peak maxima; the computed flow details and scalar mixing characteristics can be described by self-similar relations beyond a dimensionless time around 10. There are two regions in the flow field of a line thermal: a mixing region where the concentration of tracer fluid is high and the flow is turbulent and rotational with a pair of vortex eyes, and an ambient region where the concentration is zero and the flow is potential and well-described by a model of doublet with strength very close to those given by early experimental and analytical studies. The added virtual mass coeffi-cient of the thermal motion is found to be approximat     

大菱鲆幼鱼视网膜结构及不同光谱下视蛋白基因表达特征的研究

大菱鲆(Scophthalmus maximus)是中国重要的养殖鱼类之一, 其生活史经历不同的栖息地和光环境, 视觉器官结构及视觉功能也具有适应性发育特征和可塑性。本研究以不同发育阶段的大菱鲆幼鱼为对象, 探究了其视网膜结构变化、视蛋白基因的表达特征及其与光谱之间的关系。结果表明, 随着大菱鲆幼鱼的生长发育, 其视网膜的外核层逐渐变厚; 视锥视杆层的厚度变化不明显; 而内核层与神经节细胞层逐渐变薄。视紫红质基因rh1与视蛋白基因总表达量的比例上升, 由2月龄的57.35%上升到9月龄77.19%; 视锥蛋白基因与视蛋白基因总表达量的比例下降; 其中红视蛋白基因lws 由2月龄的4.49%下降至9月龄的0.13%。将7月龄的大菱鲆幼鱼用不同光谱处理75 d后, 其视蛋白基因的表达会随光谱环境的变化而发生改变。与全光谱相比, rh2b1、sws2、sws1在红、黄光下的表达量显著下降, 而rh2b1在蓝、绿光下表达显著上升、sws2在绿光下表达显著上升, 其他则变化不显著(P<0.05)。黄、绿、蓝及全光谱下rh2基因家族与视蛋白基因总表达量的比例最高, 而红光下基因rh1的表达量占比最高。大菱鲆幼鱼在不同阶段以及不同光谱处理下表现出了视蛋白基因表达的可塑性以适应不同水层的光谱环境。本研究为探究大菱鲆对光环境的适应机制及工厂化养殖光照调控技术的建立提供理论参考。     

Long-Term Effect of TMD on Vibration Control of An MDOF Offshore Fixed Platform

A three-dimensional fixed offshore platform in deep water modeled by the finite element method is studied in this paper. Analysis of the dynamic response of the MDOF structure is realized taking the non-linearity of the wave drag force and the wave-structure interaction into account. The structural response statistics, which have Gaussian distributions, are used to evaluate the vibration effect of the structure without TMD and with TMD. And an optimal method to design TMD controlling the first mode of the multi-mode structure is proposed. Moreover, the probabilities of occurrence of sea states at the platform site are considered for prediction of the long-term effect of a TMD. Simulation results demonstrate that the long-term effect of a well-designed TMD is good and the practical use is possible due to the good stability of its optimal parameters under different sea states.     

Numerical Simulation of Dynamic Response of Supple Nets

1 .Introduction Owing to the decline of capture fisheries andsaturation of traditional inshorefarms ,sea cage cul-ture as a newtype of aquaculture is developingrapidlyin China .However ,cage culture is a highriskoperation.The practice is vulnerable to natural hazards ,such as strong tides ,storms and typhoons .So,it is necessaryto assess the behavior of a sea cage exposedto waves and current before the designandinstallation of the structure . Computer simulation,due to its many advantages ,ha…     

Application of LICOM to the numerical study of the water exchangebetween the South China Sea and its adjacent oceans

1 IntroductionThe South China Sea (SCS) is the largestmarginal sea in the western Pacific (see Fig. 1). It con-nects with the SCS through the Taiwan Strait, with thePacific through the Luzon Strait, with the Sulu Seathrough the Mindoro and Balabac Straits and with theJava Sea and Andaman Sea through the Sunda Shelf(For convenience, here we refer to the section at 1.5°N,Fig. 2). It is shown that the seasonal SCS circulation ismostly affected by the summer/winter monsoon, andthe no…     

Seasonal and Spatial Variations of Total Mass Flux around Coral Reefs in the Southern Ryukyus,Japan

Total mass flux, size distribution of sediment particles and some chemical components such as total carbon (TC), total nitrogen (TN) and calcium carbonate (CaCO₃) were monitored monthly using a multi-cup sediment traps at seven coral reef sites (6 reef flat and 1 reef slope) of the Marine Protected Areas around Ishigaki, Kohama, Kuroshima and Iriomote Islands in the southern Ryukyus, Japan from September 2000 to September 2001. The size distribution of trapped sediments revealed mostly uni-modal fine sand to mud in the reef flat and gravelly to coarse sand in the reef slope. The total mass flux ranged between 0.54 to 872 gm⁻²d⁻¹, and showed a pronounced seasonality (high in summer-autumn and low in spring) at each site, which was consistent with the rainfall and typhoon regime. Exceptionally high values were observed on the reef slope (Iriomote) in February–March 2001 (1533 gm⁻²d⁻¹) owing to a large amount of bottom sediment re-suspension. On the reef flat (Todoroki South and North; Ishigaki), values obtained in July–August 2001 (872 gm⁻²d⁻¹) and August–September 2001 (800 gm^{− 2}d⁻¹) indicate the high terrestrial discharge from Todoroki River. Trapped sediment particles consist of CaCO₃ (1.2–27.1%) and a non-carbonate fraction (98.8–72.9%), which contains total carbon (4.9–26%), carbonate carbon (CO₂-C) (0.2–3.1%) and non-carbonate carbon (NC-C) (7.9–25.6%). Total nitrogen content was in the range 0.02–0.48%. TN is contained mainly in the carbonate fraction and NC-C may be contained in the non-carbonate fraction. The low TN/OC ratio of the trapped sediments suggests that they were mostly of terrestrial origin and that both fractions migrated. The high total mass flux derived from Todoroki River exceeded the threshold at which a lethal effect on coral community is caused. The results stress the importance of conducting seasonal studies of sedimentation over more than one year and at more than one location in south Japan coral reef ecosystems to gain an understanding of the processes controlling the total mass fluxes and their nutrients content, also to develop an awareness of how to prevent the damage of coral reef ecosystems and, if it does occur, to allow mitigation measures to be undertaken.     

JONSWAP谱和N谱的几个对应特征量间的关系

研究了风浪频谱ＪＯＮＳＷＡＰ谱的峰度因子γ、尖度因子Ｐｊ和Ｎ谱尖度因子ＰＮ，参变量ｐ，ｑ间的关系；并在此关系上给出了由这两种谱计算的几个对应参量间的关系，在使用不同的谱形进行特征量的计算时，应注意不同频谱间的成长因子致性，以免引起计算结果的错误。