牙鲆GHR基因Promoter区微卫星序列多态性与生长性状关系的初步研究

采用牙鲆GHR基因5'端Promoter区的1个微卫星标记,对胶南和日照2个牙鲆养殖群体进行了群体遗传多样性的研究,并探索该基因多态性位点与牙鲆生长性状之间的相关性.结果表明,2个群体在该座位的等位基因数为12和9个,有效等位基因数为6.26和5.04个,多态信息含量为0.84和0.80.2个群体该座位的Hardy-Weinberg遗传偏离指数均为正值,并没有显示出杂合子缺失,但各基因型分布频率都在一定程度上偏离Hardy-Weinberg平衡(P<0.01).连锁分析中发现,在胶南群体中,IM基因型对应的个体在全重、全长、体长、头长、体高和眼径形态学数据中均是最大的;在日照群体中,BC基因型对应的个体在全重、全长、体高、尾柄高、尾柄长和眼径数据中均是最大的;而CJ基因型对应的个体在体长和头长这两组数据中是最大的.该结果为研究GHR基因的变异对鱼类生长发育的影响及探索将该基因作为生长遗传标记的可行性奠定了实验基础.     

Wedge impact with the influence of ice

This work presents a canonical study on a wedge entering water near a single piece of ice using computational-fluid-dynamics (CFD) and a Wagner-type theoretical model with corrections for non-linear effects. Calculations for a series of conditions with ice of different sizes and locations relative to the wedge are conducted. The hydrodynamic force due to impact, the pressure distribution on the wedge surface, and the pile-up phenomenon are examined to study the role of ice in the impact process. The theoretical model is shown to be accurate and can serve as a useful method to assess slamming loads under the influence of ice. It is shown that even for the case of a small piece of ice, the slamming force on the wedge can increase by 30%.     

斑马鱼GSTM单核苷酸多态性与低温耐受性的相关分析

为分析谷胱甘肽硫-转移酶M基因(glutathione S-transferases M,GSTM)与鱼类低温耐受性的相关性,本实验运用PCR-SSCP技术研究了130尾斑马鱼(Danio rerio)GSTM基因5?UTR、3?UTR和第一内含子序列的单核苷酸多态性(Single nucleotide polymorphisms,SNPs),同时分析了筛选到的基因型与其低温耐受性状的关联性。结果显示,在其5UTR区域检测到AB、BC、AC 3种基因型个体,共A、B、C 3个等位基因,其观测杂合度和期望杂合度分别是1.000和0.570,多态信息含量为0.472,所检测群体在该座位偏离了Hardy-Weinberg平衡;第1内含子中检测到DD、DE和EE 3种基因型个体,共D、E两个等位基因,其观测杂合度和期望杂合度分别为0.408和0.477,多态信息含量为0.362,群体在该座位符合Hardy-Weinberg平衡。3'UTR区域中没有发现多态性。上述2个SNP座位与斑马鱼低温耐受性状的关联分析结果表明,5'UTR区3种基因型与低温耐受性状均没有显著相关性(χ2=4.029,P0.05)。而第1内含子3种基因型与低温耐受性状显著相关(χ2=8.498,P0.05):DD基因型在耐低温群体中占优势(50.00%),并表现为对受低温胁迫斑马鱼的保护性因素(OR=0.520,95%CI=0.255–1.061),而DE基因型在不耐低温群体中占优势(51.31%),表现为低温胁迫下斑马鱼的危险因素(OR=3.012,95%CI=1.413–6.419)。研究结果为GSTM基因SNPs位点与斑马鱼低温耐受性能关联分析提供了依据,也将为海水经济鱼类抗寒标记筛选育种提供参考。     

基于窗口速度平滑伪距的遥感平台定位算法

对浮空器遥感平台进行精密的控制定位是其完成对地观测、中继通信以及环境遥感等任务的关键。在自主地面测控系统对浮空器平台跟踪定位的基础上,给出加窗径向速度平滑伪距的多站跟踪定位方法。仿真结果表明:在GDOP值超过6的情况下,利用加窗速度平滑伪距方法仍能改善遥感平台定位的精度,而且能够有效地削弱测距链路中粗差的影响,从而自主地根据近地空间环境对浮空器进行控制。     

带丁坝型半封闭矩形海湾中M_2分潮潮波和潮流特性分析

将黄渤海海域概化为矩形海湾,山东半岛概化为垂直于海岸的巨型丁坝,形成一个带丁坝型半封闭矩形海湾。利用DELFT3D-FLOW计算模块在上述海湾中进行了M2分潮数值模拟,对其无潮点和辐射状潮流场的特性进行了分析。研究发现:考虑科氏力影响和巨型丁坝反射作用,坝前形成了明显的无潮点,但在等水深条件下并未形成辐射状潮流场,而叠加上倾斜海底地形后其得以形成;无潮点和辐射状潮流场顶端位置受水深影响明显,随着平均水深的增大,无潮点将向湾口和湾中轴线方向偏移,而辐射状潮流场顶端则向湾口方向偏移。研究结果有助于加深对带丁坝型半封闭矩形海湾中驻潮波系统形成机制和动力特性的理解和认识。     

Observer presence may alter the behaviour of reef fishes associated with coral colonies

Although underwater visual census (UVC) is the most frequently used technique for quantifying reef fish assemblages, remote video analysis has been gaining attention as a potential alternative. In the South Atlantic Ocean, Millepora spp. (class Hydrozoa) are the only branching coral species; however, little is known about the ecological role that they play for reef fish communities. We compared these two observation methods (remote video and UVC) to estimate reef fish abundance and species richness associated with colonies of the fire‐coral Millepora alcicornis at Tamandaré Reefs, Northeast Brazil. Additionally, the two different techniques were used to compare species behaviour in association with fire‐corals in order to examine the biases associated with each technique and provide useful information for behavioural ecologists studying fish–coral associations. There were no differences in reef fish abundance or species richness sampled by remote video or UVC. However, a significant difference in the behaviour of associated fish was recorded between the two methods. In the presence of a diver carrying out a UVC, fish were observed spending more time sheltered amongst the coral branches compared with passively swimming on coral colonies with the remote video technique. Specifically, on the remote video recordings agonistic interactions between fish and passive swimming accounted for 33.3% and 22.2% of the census time, respectively. By comparison, when observed by a diver fish spent 34.8% of their time sheltering amongst the coral branches. We demonstrate that both techniques are similarly effective for recording fish abundance and species richness associated with fire‐corals. However, differences were observed in the ability of each method to detect the behaviour of coral‐associated fishes. Our findings show that behavioural ecologists studying complex fish–coral associations need to ensure that their aims are clearly defined and that they choose the most appropriate technique for their study in order to minimize methodological biases.     

半潜平台浮子式系泊系统参数优化研究

通过在系泊缆中设置浮子可以改善系泊系统性能,降低平台运动响应。建立浮子式系泊系统的数值计算模型,验证浮子式系泊系统数值计算方法,详细分析浮子设计参数(设置位置和净浮力大小)变化对系泊缆张力特性与平台运动的影响规律,并根据得到的规律选择了优化的浮子系泊系统方案。最后对优化方案进行评估,表明优化方案可以显著降低系泊中的平台水平运动,尤其是低频运动,同时系泊缆张力变化不大,仍满足安全要求。研究结果可为今后浮子式系泊系统设计提供参考。     

中太平洋深海沉积物中元素组合特征及地质意义

深海沉积物中稀土(REY,包括Y)是继多金属结核、富钴结壳和热液硫化物之后又一有潜力的深海矿产资源。对中太平洋46个富稀土的沉积物样品(∑REY=(730~1 596)×10-6)和53个相对贫稀土的沉积物样品(∑REY=(324~487)×10-6)进行主微量和稀土地球化学分析,利用聚类分析和因子分析法,对元素组合特征进行了分析,划分元素组合和4个主因子。综合特征表明,2类沉积物REY的富集均与磷酸盐有关,REY含量高低取决于磷酸盐含量,但沉积物的形成具有多源多期的特点,富REY沉积物中铝硅酸岩(黏土矿物和沸石)对沉积物中磷酸盐富集REY的过程具有重要的促进作用,而在贫REY沉积物中铝硅酸岩对REY的富集意义不明显。     

Prediction of the dynamic response of a slender marine structure under an irregular ocean wave using the NARX-based quadratic Volterra series

The time series of the dynamic response of a slender marine structure was predicted in approximate sense using a truncated quadratic Volterra series. The wave-structure interaction system was identified using the NARX (Nonlinear Autoregressive with Exogenous Input) technique, and the network parameters were determined through supervised training using prepared datasets. The dataset used for network training was obtained by nonlinear finite element analysis of the slender marine structure under random ocean waves of white noise. The nonlinearities involved in the analysis were both large deformation of the structure under consideration and the quadratic term of the relative velocity between the water particle and structure in the Morison formula. The linear and quadratic frequency response functions of the given system were extracted using the multi-tone harmonic probing method and the time series of the response of the structure was predicted using the quadratic Volterra series. To check the applicability of the method, the response of a slender marine structure under a realistic ocean wave environment with a given significant wave height and modal period was predicted and compared with the nonlinear time domain simulation results. The predicted time series of the response of structure with quadratic Volterra series successfully captured the slowly varying response with reasonably good accuracy. This method can be used to predict the response of the slender offshore structure exposed to a Morison type load without relying on the computationally expensive time domain analysis, especially for screening purposes.     

Numerical algorithm for the dynamic analysis of base-isolated structures with dry friction

This paper deals with the techniques for solving ordinary differential equations with essential nonlinearity arising from the representation of frictional force by the sign function of the relative velocity. This problem is connected with dynamic behavior studies of base-isolated structures with dry friction devices.Mathematical evidence is presented concerning the inaccuracies of the solution which are connected with the representation of the frictional force by the sign function. An approximation of this sign model is made on the basis of the appropriate linearization of a small part of the frictional force function. A numerical algorithm for the dynamic analysis of base-isolated structures with dry friction is proposed. Numerical tests are carried out for establishing the exactness of the proposed techniques. The obtained results prove the accuracy of the techniques.