Growth inhibition and oxidative stress in two species of marine diatoms exposed to 1-phenylethanol

1-phenylethanol (1-PEA) is a flavor extensively used in the production of cosmetics, beverages, and food. The release of 1-PEA into coastal environments has aroused great concern. However, its potential effects on marine organisms are still unknown. In order to provide a better understanding of the ecological risks of 1-PEA in marine environments, this study determined the toxic effects of 1-PEA on two marine diatoms (Phaeodactylum tricornutum and Skeletonema costatum). The diatoms were grown in culture medium containing different concentrations of 1-PEA for 96 h. The contents of chlorophyll a, chlorophyll c, glutathione (GSH), malondialdehyde (MDA), and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), were measured at the end of the exposure period. 1-PEA was shown to significantly inhibit the growth of diatoms, with 96-h EC50 values of 257.14 mg/Land 126.46 mg/L in P. tricornutum and S. costatum, respectively. In P. tricornutum, the levels of SOD, CAT, GPx, GSH, and MDA were stimulated only when 1-PEA concentrations were close to or greater than the 96-h EC50 value. However, in S. costatum, the activities of SOD and CAT, and the syntheses of two chlorophylls were inhibited even at an exposure concentration below the 96-h EC50 value. Taken together, these findings indicate a potential ecological risk by discharging 1-PEA into coastal areas and its species-specific toxic effects on marine organisms.     

Isolation of protein from Chlorella sorokiniana CY1 using liquid biphasic flotation assisted with sonication through sugaring-out effect

Microalgae,a sustainable source of multi beneficial components has been discovered and could be utilised in pharmaceutical,bioenergy and food applications.This study aims to investigate the sugaring-out effect on the recovery of protein from wet green microalga,Chlorella sorokiniana CY1 which was assisted with sonication.A comparison of monosaccharides and disaccharides as one of the phaseforming constituents shows that the monosaccharides,glucose was the most suitable sugar in forming the phases with acetonitrile to enhance the production of protein(52% of protein).The protein productivity of microalgae was found to be significantly influenced by the volume ratio of both phases,as the yield of protein increased to 77%.The interval time between the sonication as well as the sonication modes were influencing the protein productivity as well.The optimum protein productivity was obtained with 10s of resting time in between sonication.Pulse mode of sonication was suitable to break down the cell wall of microalgae compared to continuous mode as a lower protein yield was obtained with the application of continuous mode.The optimum condition for protein extraction were found as followed:200g/L glucose as bottom phase with volume ratio of 1:1.25,10s of resting time for ultrasonication,5s of ultrasonication in pulse mode and 0.25g of biomass weight.The high yield of protein about 81% could be obtained from microalgae which demonstrates the potential of this source and expected to play an important role in the future.     

An iterative PS-InSAR method for the analysis of large spatio-temporal baseline data stacks for land subsidence estimation

South-west of Tehran, the capital city of Iran, is subjected to a high deformation rate due to excessive groundwater extractions. Persistent Scatterrer SAR Interferometry (PS-InSAR) technique is used to monitor Tehran’s deformation. Three time series data including two Sentinel-1A (S-1A) spanning from 2014 to 2017, and an ENVISAT-ASAR data stack spanning from 2004 to 2010, are analyzed. The PS-InSAR technique does not perform well on ENVISAT-ASAR due to poor selection of PS points induced by large perpendicular baselines and strong temporal decorrelation of the dataset. In this paper, a novel Iterative PSI method (IPSI) is proposed to increase the PS points which are lost in PS-InSAR technique because of the unsuccessful derivation of the absolute phase value due to an integer ambiguity. The method selects PS points based on simultaneous analysis of their amplitude and phase. Results demonstrate that the density of PSs has been increased by about 4.5 times. Line of Sight (LOS) velocities obtained from both S-1A and ENVISAT-ASAR data analysis are highly compatible with each other, indicating the reliability of the both applied methods. The maximum cumulative displacements are estimated as 39.6 cm and 88.4 cm for Sentinel-1A and ENVISAT-ASAR datasets respectively. Moreover, the subsidence area has grown in the period between the data acquisition time. The methods are successfully validated by subsidence rates obtained from precise leveling and GPS observations.     

多时相阴影轮廓差分辅助下的城市水体提取

如何避免水体提取中阴影信息与水体信息的混淆,是利用遥感数据提取城市水体信息需要解决的一个问题。本文以高分一号WFV图像及Landsat8 OLI图像为数据源,利用阴影轮廓的位置与形状在不同太阳高度角及太阳方位角下的差异性,提出一种基于多时相阴影轮廓差分的城市水体提取方法(WMSD)。以广州市天河区为试验区进行水体信息提取,同时运用NDWI、MNDWI及SWI指数法分别提取水体信息,进行精度对比分析。结果显示,本文所提出的WMSD方法分类精度超过88%,较NDWI法、SWI法及MNDWI法的水体提取精度分别提高了8.50%、9.50%及4.67%。说明基于阴影轮廓位置与形状的差异提取水体信息的方法能够较好地解决阴影与水体提取信息混淆的问题,为利用遥感数据提取城市地区水体提供了一个可行的处理方法。     

"资源一号"02C卫星影像土地利用变化检测能力分析

“资源一号”02C卫星自成功发射以来,被广泛应用于土地资源、矿产资源、地质环境调查,以及国土资源、地质灾害应急监测等应用领域。“资源一号”02C卫星是一颗填补我国高分辨率遥感数据空白的卫星,是根据国土资源主体业务需求定制的第一颗国产高分辨率业务卫星。本文以吉林省和浙江省某试验区“资源一号”02C卫星数据为例,对“资源一号”02C卫星数据融合影像进行了土地利用遥感监测变化信息检测能力的方法试验。根据试验区数据变化信息提取试验结果,对“资源一号”02C卫星影像在土地利用动态遥感监测中的变化检测能力和适用性开展了试验、分析和评价工作。     

背负式移动激光扫描系统测绘大比例尺地形图精度试验研究

将背负式移动激光扫描系统应用在测绘大比例尺地形图中,其扫描精度至关重要。本文利用徕卡Pegasus Backpack对苏州工业园园区测绘地理信息大楼进行扫描,采用Inertial Explorer、Infinity、AoTumatic Processing对点云数据进行预处理,运用RealWorks提取特征点,将特征点在MicroStation V8联图中绘制成1：500地形图。通过与传统方法绘制的1：500地形图相叠合,发现两幅地形图具有很好重合度。对地物检测点精度分析后,得到点位中误差为0.026 m,高程中误差为0.041 m。研究结果表明徕卡Pegasus Backpack满足1：500地形图测量精度要求。     

DFT对Rb+水合团簇及其相关性质的分析

运用密度泛函理论(DFT)在ωB97XD/gen (O、H元素Aug-cc-PVDZ; Rb元素Lanl2DZ)基组水平上研究了[Rb(H_2O)_n]~+(n=1～12)水合团簇的结构和光谱学性质。研究结果表明,通过分析气相和液相中的稳定能以及水合团簇可知,稳定构型出现在8配位的团簇中。第一配位层水分子数为8时形成Rb~+第一近似饱和水合层。此外,拉曼光谱显示[Rb(H_2O)_n]~+(n=1～12)水合团簇中O-H伸缩振动峰随着水分子数增加发生移动和加宽,Rb~+发生水合时,O-H对称和非对称伸缩振动发生红移,随着水合数的增多,频率蓝移。此外,文中对拉曼光谱中O-H振动的频率位置结合团簇结构进行了说明。     

基于TCP-InSAR的采动区铁路动态沉降监测与时序分析

为高效获取采动区长时间序列形变,监控煤炭开采对矿区铁路的影响,研究一种基于TCP-InSAR(temporarily coherent point interferometric synthetic aperture radar)的采动区铁路形变监测方法。该方法根据时间序列SAR影像间的相干性,选取临时相干点构建Delaunay三角网,并通过离群值探测去除具有相位模糊度的TCP间的弧段,最后采用最小二乘解算得到区域地表变形。实验使用2016-10～2017-04时间段内的15景Sentinel-1A数据,利用TCP-InSAR技术获得某矿区的铁路形变。结果表明,受采动影响,该时间段内铁路最大下沉值为95mm,最大倾斜坡度为0.37‰。利用TCP-InSAR技术可实现采动区内铁路长时间的动态形变监测。     

非等间距GM(1,1)模型的总体最小二乘算法及其病态问题

在非等间距GM(1,1)模型中,系数矩阵中有无误差的常数项和有误差的随机项,并且系数矩阵与观测向量误差同源,即系数矩阵与观测向量中有相同的元素存在,这些相同元素应该有相同的改正数,为此本文推导了一种适合非等间距GM(1,1)模型求解的总体最小二乘算法。同时,考虑到非等间距GM(1,1)模型中存在病态问题时影响总体最小二乘计算结果的稳定性,提出对系数矩阵常数列乘以某一常数的方法,以改善病态问题。     

大连长兴岛海域疏浚区GF-1 WFV影像悬浮物遥感反演研究

利用2017年8-9月期间大连长兴岛海域实测遥感反射率、悬浮物浓度及GF-1 WFV数据,研究了该区域悬浮物浓度的遥感反演算法,发现遥感反射率与悬浮物浓度的散点图存在两种不同的变化趋势,将2008-2015年期间辽东湾内其它海域的数据与长兴岛海域数据进行叠加后,可初步推断产生两种变化趋势的主要原因在于疏浚区与非疏浚区悬浮物的粒径大小不同,导致其光学特性和反演算法也存在差异。将两种算法分别应用于2017年9月20日辽东湾及长兴岛近岸海域的GF-1 WFV4影像,发现采用非疏浚区算法反演长兴岛海域悬浮物浓度时,出现明显的低估现象,而疏浚区算法则能较好地反映出疏浚施工过程中悬浮物的分布规律,具有较好的工程应用价值。