裙带菜配子体基因工程选择标记的研究

研究了裙带菜(Undaria pinnatifida)配子体对除草剂草丁膦的敏感性以及温度对草丁膦处理效果的影响。结果表明,裙带菜配子体对草丁膦敏感;统计学分析得出了草丁膦在不同温度条件下处理裙带菜配子体的半致死浓度(LD50)及95%可信限;温差达到10℃时,相同处理时间各组内不同处理温度样品的LD50之间呈现显著差异,第6天的LD50由10℃组的15.78 mg/L降为20℃组的11.56 mg/L和25℃组的10.33 mg/L。研究发现可以尝试采用草丁膦作为裙带菜配子体遗传转化的选择试剂。     

长江口徐六泾悬浮细颗粒泥沙絮凝体特性

2003年6月14日-22日, 利用现场激光粒度仪LISST-100在不扰动颗粒物的情况下, 于长江口徐六泾定点连续观测了洪季大、小潮表层粘性悬浮细颗粒泥沙絮凝体的实有粒径、体积浓度, 配合OBS-3A现场测量的悬沙浓度计算了现场絮凝体的有效密度和静水沉速.观测结果显示, 徐六泾大、小潮表层絮凝体体积浓度、粒径、有效密度和静水沉速的平均值分别为98.0 μl/L、39.8 μm、1173 kg/m^3、1.14 mm/s和70.8 μl/L、64.4 μm、919 kg/m^3、2.32 mm/s.研究表明： ①长江口徐六泾表层絮凝体体积浓度主要受水流流速影响, 再悬浮现象明显, 体积浓度过程线滞后流速过程线, 落潮期间滞后10-30 min, 涨潮则滞后30-50 min; ②小于一定流速时絮凝体平均粒径随流速增大而增大, 大于一定流速时絮凝体平均粒径则随流速增大而减小, 徐六泾大、小潮表层絮凝体在50 cm/s的垂线平均流速时出现平均粒径与垂线平均流速关系的转折; ③徐六泾大、小潮表层絮凝体平均粒径在体积浓度75 μl/L时出现平均粒径与体积浓度关系的转折, 体积浓度小于75 μl/L时粒径随体积浓度增加而增大, 超过75 μl/L时粒径随体积浓度的增加变化不明显; ④絮凝体有效密度由粒径大小控制, 粒径大, 有效密度小, 反之亦然, 粒径和有效密度共同决定絮凝体静水沉速, 有效密度和沉速与平均粒径之间均存在良好的幂指数关系.     

冬季和春季长江口及其近海水域浮游病毒丰度的分析

采用荧光显微技术,对2006年长江口及近海水域20个站点的表层及10m层或潜水体冬、春两季的浮游病毒丰度进行了检测,对浮游病毒丰度在季节（冬、春两季）、水平分布和垂直分布上的变化进行了探讨.调查区浮游病毒丰度在冬、春季节上并无明显差异,但在水平分布上存在很大差异,河口区浮游病毒直接检测量（Virus Direct Count, VDC）达到10^7个/ml,近海水域VDC为10^6个/ml,河口区的浮游病毒丰度都明显高于近海水域病毒丰度 （P＜0.01）.在垂直分布上,冬、春两季长江口水域水深小于10m的站位,表层浮游病毒丰度与底层病毒丰度无明显差别,水深大于10m的站位,表层水样的浮游病毒丰度都高于10m水层病毒丰度,说明长江口浮游病毒的垂直分布与站位总水深有关.还通过比较各站点VDC与叶绿素a含量的数据,分析了二者之间的相关性：冬季浮游病毒丰度与叶绿素a含量成正相关性;春季浮游病毒丰度与叶绿素a含量成负相关性,但病毒丰度受叶绿素a含量的影响仅为10%-11%.     

Impacts of Aerosol-Radiation Interactions on the Wintertime Particulate Pollution under Different Synoptic Patterns in the Guanzhong Basin,China

The effects of aerosol–radiation interactions(ARI) are not only important for regional and global climate, but they can also drive particulate matter(PM) pollution. In this study, the ARI contribution to the near-surface fine PM(PM2.5)concentrations in the Guanzhong Basin(GZB) is evaluated under four unfavorable synoptic patterns, including "northlow", "transition", "southeast-trough", and "inland-high", based on WRF-Chem model simulations of a persistent heavy PM pollution episode in January 2019. Simulations show that ARI consistently decreases both solar radiation reaching down to the surface(SWDOWN) and surface temperature(TSFC), which then reduces wind speed, induces sinking motion,and influences cloud formation in the GZB. However, large differences under the four synoptic patterns still exist. The average reductions of SWDOWN and daytime TSFC in the GZB range from 15.2% and 1.04°C in the case of the"transition" pattern to 26.7% and 1.69°C in the case of the "north-low" pattern, respectively. Furthermore, ARI suppresses the development of the planetary boundary layer(PBL), with the decrease of PBL height(PBLH) varying from 18.7% in the case of the "transition" pattern to 32.0% in the case of the "north-low" pattern. The increase of daytime near-surface PM2.5 in the GZB due to ARI is 12.0%, 8.1%, 9.5%, and 9.7% under the four synoptic patterns, respectively. Ensemble analyses also reveal that when near-surface PM2.5 concentrations are low, ARI tends to lower PM2.5 concentrations with decreased PBLH, which is caused by enhanced divergence or a transition from divergence to convergence in an area. ARI contributes 15%–25% toward the near-surface PM2.5 concentrations during the severe PM pollution period under the four synoptic patterns.     

Grey relational analysis on the relation between marine environmental factors and oxidation-reduction potential

The effects of marine environmental factors-temperature(T),dissolved oxygen(DO),salinity(S) and pH-on the oxidation-reduction potential(ORP) of natural seawater were studied in laboratory.The results show an indistinct relationship between these four factors and the ORP,but they did impact the ORP.Common mathematical methods were not applicable for describing the relationship.Therefore,a grey relational analysis(GRA) method was developed.The degrees of correlation were calculated according to GRA and the va...     

Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses

The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder Sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes,12 confirmed hybridization of the paternal and matemal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish.     

Adaptation management of mountain tourism service: the case of the source regions of the Yangtze and Yellow River

Mountain areas are often rich in ecological diversity and recreational opportunities. Mountain tourism is thought to be an effective and important means for maintaining and expanding rural economies and, thus, improving the living conditions of rural societies. As mountain tourism service research is a professional field with several disciplines involved, a multi-disciplinary management pIatform is needed and it facilitates participation in sustainable mountain development by diverse stakeholders. With the source regions of the Yangtze and the Yellow River as a case study, this paper presents a conceptual framework for an adaptation management of mountain tourism services according to technical, policy, social and economic dimensions. The framework is based on a vulnerability assessment of mountain ecosystems, and can serve as a reference for the development of tourism service in other mountain areas.     

人工育珠对三角帆蚌(Hyriopsis cumingii)遗传基因影响的初步研究

采用酚-氯仿和试剂盒两种提取法对三角帆蚌怀珠群与非怀珠群各6个个体进行基因组DNA的提取,然后在优化RAPD(随机扩增多态性DNA)检测条件基础上,从80个随机引物中筛选出12个扩增较好且多态性强的引物进行RAPD扩增,产物通过水平板琼脂糖凝胶电泳和垂直板聚丙烯酰胺凝胶电泳两种方法进行检验并对DNA的多态性进行分析。结果显示在怀珠群和非怀珠群检测到的位点数、多态位点比例、平均Shannon多态性信息指数、平均Nei's基因多样性指数、群体内个体间平均遗传相似率和平均遗传距离分别为100、33%、0.1927、0.1324、0.904、0.096,95、47.37%、0.2711、0.1879、0.861、0.139,群体间的平均遗传距离为0.821,非怀珠群的变异性大于怀珠群;本研究还获得了两群体各自的特异扩增谱带及两群体间表达差异较大谱带,这些位点很可能是由人工育珠所引起。根据MEGA4.0软件的UPGMA和NJ程序构建的分子系统树可直观地将两群体分开。     

GISVlog模型的设计与实现

本文以地理信息系统(GIS为基础,分析了地理信息系统沃客(GISVlog,)形成的两个背景——网络新技术提高GIS生命力和沃客(Vlog,Vedio log的缩写)为网络增值;分别论述了GISVlog形成对GIS发展和Vlog发展的意义;在普通GIS的系统功能结构和Vlog的系统功能结构的基础上构建GISVlog的框架结构并加以实现;论文最后展望了GISVlog的发展前景,提出GISVlog是网络搜索技术新的研究方向。     

Non-stationary covariance function modelling in 2D least-squares collocation

Standard least-squares collocation (LSC) assumes 2D stationarity and 3D isotropy, and relies on a covariance function to account for spatial dependence in the observed data. However, the assumption that the spatial dependence is constant throughout the region of interest may sometimes be violated. Assuming a stationary covariance structure can result in over-smoothing of, e.g., the gravity field in mountains and under-smoothing in great plains. We introduce the kernel convolution method from spatial statistics for non-stationary covariance structures, and demonstrate its advantage for dealing with non-stationarity in geodetic data. We then compared stationary and non- stationary covariance functions in 2D LSC to the empirical example of gravity anomaly interpolation near the Darling Fault, Western Australia, where the field is anisotropic and non-stationary. The results with non-stationary covariance functions are better than standard LSC in terms of formal errors and cross-validation against data not used in the interpolation, demonstrating that the use of non-stationary covariance functions can improve upon standard (stationary) LSC.