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21.
固定化鲅鱼乙酰胆碱酯酶的制备及部分性质测定 总被引:3,自引:0,他引:3
酶的固定化是酶传感器制备过程中重要的1个环节.研究采用直接共价法固定鲅鱼乙酰胆碱酯酶(AChE).制备方法为:0.1g CNBr活化的Sepharose 4B凝胶用1mmol/L的HCl充分溶胀后,与活力为10U的AChE溶液混合,于4℃下150r/min振荡8h.所制备的固定化酶活力回收率较高(96%),对pH值和温度变化的适应能力均优于非固定化酶;在3个月保存期内,前者的活力损失13%,而后者的活力则下降89%.这说明固定过程能够大大提高AChE的抗逆性和保持酶活力的稳定,有利于酶传感器的制备. 相似文献
22.
以1,4—苯二酚和不同的短链脂肪酸为基本原料,经溴代、加成、形成酰卤和酰化等反应,合成了六种新的溴化脂肪酸—2,3,5,6—四溴—1,4—苯二酯。通过对产物的碳、氢、溴等元素的定量分析,以及红外吸收光谱和氢核磁共振谱的研究,验证了化合物的结构。 相似文献
23.
设计出双级看门狗(WATCHDOG)实用电路.能可靠地监视微机的运行状态,保证软件在强干扰的恶劣环境下能正常运行。 相似文献
24.
在Gaussian波场基础上,推导出以P-M谱和平均JONSWAP谱代表的充分成长和有限风区的深水风浪平均波长与平均波周期之间的关系为.推导中运用了Rice跨零点问题的解并通过滑动时间平均技术估计4阶谱矩.在风浪水槽进行了实验,实验结果与有限风区下推导出的关系相比较,符合较好. 相似文献
25.
中国第22次南极科学考察(2005年11月至2006年3月)期间,测定了南极普里兹湾海域5个站位的从表层至150 m水深的不同层位水样中溶解态和颗粒态234Th,238U的放射性比活度以及颗粒有机碳.利用234Th/238U在上层水体中的不平衡,计算了南极普里兹湾上层水体中234Th的平均停留时间和输出通量.结果显示,随着纬度的增加,上层水体中颗粒态和溶解态234Th的平均停留时间总体趋向减小,并在中纬度站位出现了最低值,分别为1~8和29~48 d,而颗粒态和溶解态234Th的输出通量则在中纬度站位出现了最大值,分别为21~38和26~39 dpm/(m3·d).运用箱型清除模式,利用两种不同的方法估算了各水柱中从真光层底部输出的POC通量,平均值分别达到104.7 mmol/(m2·d)(E法)和120.6 mmol/(m2·d)(B法),表明南极普里兹湾夏季存在很高的新生产力,它将会对该海域碳的生物泵过程产生重要作用. 相似文献
26.
Eurgain H. John Sonia D. Batten Roger P. Harris Graeme C. Hays 《Journal of Sea Research》2001,46(3-4)
Zooplankton sampling has been carried out by the Continuous Plankton Recorder (CPR) survey since the 1930s enabling the study of long-term changes in plankton populations, the elucidation of seasonal patterns of abundance, and more recently providing zooplankton biomass estimates for ecosystem models. Data for zooplankton abundance collected by CPR tows in the Western English Channel (between 1988 and 1998) were compared to vertically integrated samples collected from station L4 off Plymouth, UK. Comparisons were made for locally abundant copepods (including Acartia, Calanus, Para/Pseudocalanus, Centropages, Oithona and Temora) collected by CPR and WP-2 nets. All dominant species recorded at L4 were also common to the CPR data. However, the position of the taxa in the two datasets was not equivalent. Seasonal cycles revealed by CPR data were significantly similar to those recorded throughout the water column at L4 for most taxa. However, absolute levels of abundance differed for the two datasets: abundances were underestimated by CPR samples when compared to those of vertically integrated samples by a factor of between 2 and 35, with the exception of Centropages. The differing mesh sizes (200 and 270 μm) of the WP-2 net and CPR mesh could only partially explain these differences in abundance, implying that the behaviour of individual taxa and their depth in the water column also influenced the abundance recorded. 相似文献
27.
Michael Riedel 《Marine Geophysical Researches》2007,28(4):355-371
Two single-channel seismic (SCS) data sets collected in 2000 and 2005 were used for a four-dimensional (4D) time-lapse analysis
of an active cold vent (Bullseye Vent). The data set acquired in 2000 serves as a reference in the applied processing sequence.
The 4D processing sequence utilizes time- and phase-matching, gain adjustments and shaping filters to transform the 2005 data
set so that it is most comparable to the conditions under which the 2000 data were acquired. The cold vent is characterized
by seismic blanking, which is a result of the presence of gas hydrate in the subsurface either within coarser-grained turbidite
sands or in fractures, as well as free gas trapped in these fracture systems. The area of blanking was defined using the seismic
attributes instantaneous amplitude and similarity. Several areas were identified where blanking was reduced in 2005 relative
to 2000. But most of the centre of Bullseye Vent and the area around it were seen to be characterized by intensified blanking
in 2005. Tracing these areas of intensified blanking through the three-dimensional (3D) seismic volume defined several apparent
new flow pathways that were not seen in the 2000 data, which are interpreted as newly generated fractures/faults for upward
fluid migration. Intensified blanking is interpreted as a result of new formation of gas hydrate in the subsurface along new
fracture pathways. Areas with reduced blanking may be zones where formerly plugged fractures that had trapped some free gas
may have been opened and free gas was liberated. 相似文献
28.
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30.
Kazuo Abe 《Journal of Oceanography》2002,58(4):577-588
The relationship between dissolved cadmium (Cd) and phosphate (PO4) was examined at three stations in the subtropical area near the Ryukyu Islands in May 1999. Preformed PO4 was obtained using the Redfield ratio in order to separate the surface water and the other layers in this study area. Almost
0 μM (−0.043 μM to 0.094 μM) was estimated in the layers above 300 m and 250 m at Sts. 1 and 3 and at St. 2, respectively.
Up to these depths, water was considered to be uniform, and these layers were defined as the surface water in this study area.
In the surface water, the slopes of the regression lines of the Cd-PO4 plot were 0.162, 0.156, and 0.226 (nM/μM) at Sts. 1, 2, and 3, respectively, and these values were much closer to the estimated
regenerated ratio of Cd to PO4 from the Apparent Oxygen Utilization (AOU)-Cd/PO4 plots, which was 0.197 (nM/μM) in this study area. Below surface layers, the slopes of the Cd-PO4 plot changed to 0.371, 0.352, and 0.362 (nM//μM) at Sts. 1, 2, and 3, respectively. In the relationships between Cd and PO4, clear deviations or kinks were observed at three stations at a PO4 concentration of approximately 0.2 μM in the plot, which was attributable to the discontinuity of surface water and the other
layers across the North Pacific subtropical mode water. In studies of the interaction between surface water and biogenic particles
concerning the Cd/PO4 ratio, separate analyses of seawater (surface water and the other layers) should be carried out to obtain the individual
surface water ratio because the Cd/PO4 ratio in the surface water is expected to differ from that of the underlying water. Furthermore, the biological fractionation
of these constituents is based on the surface water ratio.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献