施氏鲟Asnanos1基因序列分析及其在雌雄不同组织中表达

本研究首先从施氏鲟(Acipenser schrenckii)性腺转录组中获得nanos1(Asnanos1)基因全长c DNA,并对其序列的准确性和特征分别进行PCR验证和生物信息学分析。进一步利用荧光定量RT-PCR技术检测Asnanos1基因在雌、雄施氏鲟的性腺、心脏、鳃、脑、肾、肝脏、脾脏等组织中的表达量。利用荧光定量RT-PCR技术检测Asnanos1基因在雌、雄施氏鲟的性腺、心脏、鳃、脑、肾、肝脏、脾脏等组织中的表达量。结果显示:Asnanos1的c DNA全长为1 389 bp,其中,5′非编码区(UTR)为414 bp,3′UTR为279 bp,ORF为696 bp。该基因共编码231个氨基酸。Asnanos1基因在施氏鲟除心脏以外的各组织中均有表达,在雌、雄鱼的鳃、脑、肾、肝脏、脾脏等组织中表达量无显著差异,但在卵巢中的表达量显著高于其他各组织(P0.05)。所得到的结果可为人工培育全雌施氏鲟苗种提供一些基础资料。     

黄河三角洲湿地资源生态旅游开发利用研究

在研究黄河三角洲自然环境演变的基础上,对黄河三角洲湿地生态旅游资源的特征及其生态旅游开发潜力进行了分析研究,并针对黄河三角洲湿地生态旅游开发方式和途径以及开发中应注意的问题提出了建议。     

三苯基氯化锡对扁藻细胞超微结构的影响

于１９９２年４月－１９９２年６月以扁藻为材料进行半抑制深度三苯基氯化锡处理７２ｈ实验，用透射电镜观察观察中毒状态扁藻细胞超微结构的变化。结果表明，线粒体发生水肿，内脊局部瓦解，基质内出现由双层膜包围的同心质膜轮；叶绿体内光合片层的网状结构被破坏，光合片层上下重叠成厚块状；蛋白核中央髓部肿胀，淀粉鞘破裂成多角形淀粉板，并分散在髓部周围；叶绿体基质中出现大量淀粉粒，眼点因受淀粉粒挤压而由藻体中下部移至     

Neutron background in large-scale xenon detectors for dark matter searches

Simulations of the neutron background for future large-scale particle dark matter detectors are presented. Neutrons were generated in rock and detector elements via spontaneous fission and (α,n) reactions, and by cosmic-ray muons. The simulation techniques and results are discussed in the context of the expected sensitivity of a generic liquid xenon dark matter detector. Methods of neutron background suppression are investigated. A sensitivity of 10⁻⁹–10⁻¹⁰ pb to WIMP-nucleon interactions can be achieved by a tonne-scale detector.     

西风爆发事件对暖池厄尔尼诺和冷舌厄尔尼诺的作用——一个概念模型得到的结果

本文通过1个概念模型讨论了西风爆发事件对暖池厄尔尼诺与冷舌厄尔尼诺两种事件的作用,并讨论了它们对应的物理机制。结果表明,由西风爆发所诱导的纬向平流异常和海洋波动对厄尔尼诺的多样性有着至关重要的作用。由于模型包含了最基本的海-洋与大气物理变量及其相互作用机制,本结果可为解释西风爆发事件对厄尔尼诺多样性影响研究提供最基本的框架。     

利用ND1、ITS1序列研究三种背角无齿蚌（Anodontawoodiana）分类学问题

利用ND1、ITS1序列差异对采自太湖、在形态上被认为是背角无齿蚌、圆背角无齿蚌、椭圆背角无齿蚌的三种个体进行鉴定,并与从NCBI中下载到的同属不同种褶纹冠蚌的ND1、ITS1序列进行对比,分析了基于Kimura2-parameter模型参数得到的遗传距离,并构建了NJ树。结果表明,本研究中所有背角无齿蚌种间遗传距离变...     

Comparison of flow patterns in the near wake of a circular cylinder and a square cylinder placed near a plane wall

The flow patterns in the near wake of a cylinder (either circular or square in shape, D=25 mm) placed in the proximity of a fully developed turbulent boundary layer (thickness δ=0.4D) are investigated experimentally using particle image velocimetry (PIV). The effects of changing the gap height (S) between the cylinder bottom and the wall surface, over the gap ratio range S/D=0.1–1.0, have been investigated. The results show that both the ensemble-averaged and instantaneous flow fields are strongly dependent on S/D. The flow patterns for the two types of cylinders share many similarities with respect to the change in S/D, such as the reduced recirculation length and increased velocity fluctuation in the near wake with increasing S/D, as well as the trend of suppression of vortex shedding at small S/D and onset of vortex shedding at large S/D. However, developments of the shear layers, in terms of wake width, flow curvature, etc., are considerably different for these two types of cylinders. In general, the wake development and momentum exchange for the square cylinder are slower those for the circular cylinder at the same gap ratio. Correspondingly, it is shown that the periodic vortex shedding is delayed and weakened in the case of square cylinder, as compared to that of the circular cylinder at the same S/D.     

基于差值权重和快速傅立叶变换的“北京一号”卫星影像融合方法研究

提出一种基于差值权重和快速傅立叶变换的影像融合方法--DWF,对"北京一号"小卫星的全色和多光谱影像进行融合.该算法根据卫星影像的成像过程,通过傅立叶变换获取全色影像的高频、低频信息和多光谱影像在HIS颜色空间中分量的低频信息,由这3种信息通过基于差值权重的决策计算获得融合影像的空间信息.实验采用PCA、GS、DWF以及基于小波的HIS变换的融合方法,结果表明DWF方法优于其他融合方法,比较适合于"北京一号"卫星的全色与多光谱影像融合.     

NEW 10BE COSMOGENIC AGES FROM THE VIMMERBY MORAINE CONFIRM THE TIMING OF SCANDINAVIAN ICE SHEET DEGLACIATION IN SOUTHERN SWEDEN

The overall pattern of deglaciation of the southern part of the Scandinavian Ice Sheet has been considered established, although details of the chronology and ice sheet dynamics are less well known. Even less is known for the south Swedish Upland because the area was deglaciated mostly by stagnation. Within this area lies the conspicuous Vimmerby moraine, for which we have used the terrestrial cosmogenic nuclide (¹⁰Be) exposure dating technique to derive the exposure age of six glacially transported boulders. The six ¹⁰Be cosmogenic ages are internally consistent, ranging from 14.9 ± 1.5 to 12.4 ± 1.3 ka with a mean of 13.6 ±0.9 ka. Adjusting for the effects of surface erosion, snow burial and glacio-isostatic rebound causes the mean age to increase only by c. 6% to c. 14.4± 0.9 ka. The ¹⁰Be derived age for the Vimmerby moraine is in agreement with previous estimates forthe timing of deglaciation based on radiocarbon dating and varve chronology. This result shows promise for further terrestrial cosmogenic nuclide exposure studies in southern Sweden.     

一株H_5N_1亚型禽流感病毒株NA基因的克隆与序列分析

用RT-PCR方法从1个H5N1亚型禽流感病毒分离株A/Chicken/Guangdong/DH/1997扩增NA基因cDNA片段,将其克隆至pMD18-T载体,获得重组质粒pMD-NA,并对其核苷酸序列进行测定和分析。结果表明,该毒株的NA基因长度为1350bp,编码449个氨基酸,与其它H5N1亚型AIV分离株的核苷酸序列同源性为97.0%～99.4%,氨基酸序列同源性为97.7%～99.1%,提示禽流感病毒NA基因保守性较高。NA基因氨基酸序列的聚类分析表明该毒株与来自香港的A/Pheasant/HK/FY155/01和A/Ch/HK/FY150/01两个分离株处于同一进化枝,亲缘关系较近。