Effect of growth phase on the fatty acid compositions of four species of marine diatoms

The fatty acid compositions of four species of marine diatoms (Chaetoceros gracilis MACC/B13, Cylindrotheca fusiformis MACC/B211, Phaeodactylum tricornutum MACC/B221 and Nitzschia closterium MACC/B222), cultivated at 22 ℃±1 ℃ with the salinity of 28 in f/2 medium and harvested in the exponential growth phase, the early stationary phase and the late stationary phase, were determined. The results showed that growth phase has significant effect on most fatty acid contents in the four species of marine diatoms. The proportions of 16:0 and 16: 1n-7 fatty acids increased while those of 16 :3n-4 and eicosapentaenoic acid (EPA) decreased with increasing culture age in all species studied. The subtotal of saturated fatty acids (SFA) increased with the increasing culture age in all species with the exception of B13. The subtotal of monounsaturated fatty acids (MUFA) increased while that of polyunsaturated fatty acids (PUFA) decreased with culture age in the four species of marine diatoms. MUFA reached their lowest value in the exponential growth phase, whereas PUFA reached their highest value in the same phase.     

空气滤膜采集大气浮尘样品中多元素的测定

用氢氟酸-盐酸-硝酸-高氯酸体系对空气滤膜上附着的浮尘样品进行分解,电感耦合等离子体发射光谱法、电感耦合等离子体质谱法同时测定溶液中15个主量和微量元素铁、铜、锌、镍、镧、铈、锂、锰、铬、镉、钴、铅、钍、铊、铀。将国家一级标准物质模拟浮尘样品加入空白滤膜进行测定,结果与标准值相符。各元素4次测定结果的相对标准偏差(RSD)小于5%。     

磺酸自硬砂引起球铁件表层异常组织的研究

通过实验研究,用甲苯磺酸作催化剂的呋喃树脂自硬砂与稀土镁作催化剂生产的球铁件,通常在其铸件表层出现异常组织。提出导致异常组织产生的原因,同时又降低了试件的冲击值,并且采用反应型或屏蔽型涂料将可防止球铁件表层异常组织缺陷,为生产性能良好的球铁件提供技术依据。     

Morphological and toxicity characteristics of Pseudo-nitzschia pungens strain PP0201-01 isolated from the East China Sea

The morphology and toxicity ofPseudo-nitzschia strain PP0201-01, isolated near the estuary of Changjiang (Yangtze) River in Year 2002 and tentatively identified asPseudo-nitzschia pungens, were studied. The strain was identified morphologically asP. pungens using scanning electronic microscope (SEM). The analytical results of LC-PDA (liquid chromatography with photodiode array detector), LC-MS (liquid chromatography with mass detector), and the toxicity data from mouse bioassay, showed that no domoic acid was produced by the strain. However, the mechanism of domoic acid production byPseudo-nitzschia species is complicated. More works are needed to assess the toxic species and to evaluate the potential risk of amnesic shellfish poisoning (ASP) in China. Supported by the projects of Ministry of Science and Technology (No. 2001CB409704 and No. 2001BA804A20), and the innovation project of the Institute of Oceanology, CAS (No. 200223107).     

马氏珠母贝TRACP基因的克隆及组织表达

【目的】克隆马氏珠母贝(Pinctada martensii)抗酒石酸酸性磷酸酶(Tartrate resistant acid phosphatase,TRACP)基因,分析该基因在不同组织中的表达模式。【方法】用RACE技术克隆得马氏珠母贝TRACP基因(PmTRACP),用实时荧光定量PCR分析该基因在外套膜、闭壳肌、足、性腺、珍珠囊、肝胰腺和鳃中的表达。【结果与结论】PmTRACP基因长度为2 034 bp,开放式阅读框972 bp,编码323个氨基酸,5′UTR长度为27 bp,3′UTR长度为1 035 bp。预测PmTRACP分子质量约为36.39 ku,理论等电点为5.97。该基因含有一个钙调神经磷酸酶样磷酸酯酶结构域。PmTRACP与其他物种TRACP的同源性为48%～65%,与长牡蛎(Crassostrea gigas)TRACP的同源性最高,同时D³²、D⁷⁰、Y⁷³、N¹⁰⁸、H²⁰³、H²¹²、H²³⁷、H²³⁹等8个氨基酸活性位点和N¹¹⁴糖基化位点在不同物种TRACP中高度保守。PmTRACP与长牡蛎TRACP的亲缘关系最近。PmTRACP在马氏珠母贝各个组织均有表达,且在肝胰腺和珍珠囊中高表达。     

柠檬酸发酵中的限制性因子

回顾柠檬酸发酵生化机制的发展历史，综合多年来的研究成果和结合生产实践中出现的问题，推断最有可能的柠檬酸发酵途径。影响柠檬酸发酵的限制性因子有Ｆｅ２＋、Ｍｎ２＋、Ｍｇ２＋、Ｋ＋、Ｐｉ、ＡＤＰ、ＡＴＰ、ＧＴＰ、ＣＯ２等，它们分别作用于发酵的关键酶顺乌头酸酶、丙酮酸羧化酶、柠檬酸合成酶、丙酮酸激酶等。生产实践中应该根据不同菌株而选择控制Ｆｅ２＋、Ｍｇ２＋、Ｍｎ２＋，Ｋ＋等金属离子。     

厦门酸雨与气象要素的关系及污染源的分析

根据厦门市１９８３－１９９１年９年春雨，梅雨及台风雨酸度的观测结果，结合各场雨水同步观测的气象要素资料，分析各类型雨水酸度与气象要素的关系。同时依降水的酸化原理估计了外来源及局的地源对厦门酸雨形成的贡献。     

Limnology of Opal Lake

Opal Lake, a cold, acid lake on the central volcanic plateau of the North Island, New Zealand, had an average pH of 4.3 and was highly eutrophic. The biota was less diverse than in neighbouring near‐neutral waters. Macrophytes were rare; phytoplankton were mainly represented by the Chlorophyceae, and there were only 4 zooplankton species. The mean standing crop of macroinvertebrates was 6240 per square metre; this was higher than in other eutrophic lakes in the region and comprised more than 98% chironomid larvae represented by 4 species. Molluscs were absent. The fauna was more diverse than in more acid lakes of the .central volcanic plateau.     

The glacial and interstadial sediments at the Burn of Benholm,Kincardineshire: evidence for onshore pre‐Devensian ice movement in northeast Scotland

Pebbly clays and diamictons containing marine shell fragments and peat lenses exposed beneath subglacially deposited Late Devensian till at the Burn of Benholm provide new insights into the glacial history of Quaternary sequences in eastern Scotland. The peat yielded pollen of interstadial affinity (including Bruckenthalia spiculifolia) and non‐finite radiocarbon dates. Comparisons with other pre‐Late Devensian pollen records in northern Scotland suggest that the peat lenses are remnants of an Early Devensian interstadial deposit, of Oxygen Isotope Substage 5c or 5a age. Reworked faunal assemblages in the shelly sediments include Quaternary marine molluscs of low boreal aspect, as well as Mesozoic and Palaeozoic microfossils. Amino acid ratios from fragments of Arctica islandica suggest that the shells are of Oxygen Isotope Stage 9 age or older. The fabric and composition of the shelly sediments are consistent with their emplacement as deformation till during the onshore movement of glacially transported rafts of marine sediment. Folded and sheared contacts between the shelly deposits, peat lenses and the overlying Late Devensian till indicate that the fossiliferous sediments were glacitectonised during the main Late Devensian glaciation, when ice moved from Strathmore and overrode the site from the southwest. British Geological Survey. © NERC 2000.     

Physiological acclimation strategies of riparian plants to environment change in the delta of the Tarim River,China

The occurrence and development of riparian forests, which were mainly dominated by mesophytes species related closely with surface water. Since there was no water discharged to the lower reaches of Tarim River in the past three decade years, the riparian forests degrade severely. The groundwater table, the saline content of the groundwater, as well as the content of free proline, soluble sugars, plant endogenous hormones (abscisic acid (ABA), and cytokinins (CTK)) of the leaves and relative rates of sap flow of the Populus euphratica Oliv. (arbor species), Tamarix ramosissima Ldb. (bush species), and Apocynum venetum L. (herb species) were monitored and analyzed at the lower reaches of the Tarim River in the study area where five positions on a transect were fixed at 100 m intervals along a sampling direction from riverbank to the sand dunes before and after water release. The physiological responses and acclimation strategies of three species to variations in water and salinity stress were discussed. It was found that A. venetum population recovered to groundwater table ranging from −1.73 to −3.56 m, and when exposed to saline content of the groundwater ranging from 36.59 to 93.48 m mol/L; P. euphratica appeared to be more sensitive to the elevation of groundwater table than the A. venetum and T. ramosissima at groundwater table ranging from −5.08 to −5.80 m, and when exposed to saline content of the groundwater ranging from 42.17 to 49.55 m mol/L. T. ramosissima tended to be the best candidate species for reclamation in this hyper-arid area because it responded to groundwater table ranging from −1.73 to −7.05 m, and when exposed to saline content of the groundwater ranging from 36.59 to 93.48 m mol/L. These results explained the distribution patterns of desert vegetation in the lower reaches of the Tarim River. Understanding the relationships among ecological factors variables, physiological response and acclimation strategies of plant individuals could provide guidance to sustainable management, reclamation and development of this and similar regions.