日本囊对虾Marsupenaeus japonicus)组织蛋白酶D基因克隆及表达分析

组织蛋白酶D是溶酶体天冬氨酸蛋白酶家族的主要成员,广泛参与动物机体细胞内蛋白质的降解过程,对维持细胞稳态和正常代谢具有重要的作用。为研究组织蛋白酶D在甲壳动物非特异性免疫和幼体发育过程中的作用,本研究采用RACE技术首次克隆得到日本囊对虾组织蛋白酶D基因cDNA序列,命名为MjCatD,其中开放阅读框长为1161 bp,编码386个氨基酸残基。序列分析和同源建模显示该基因编码的蛋白含有保守的N-糖基化位点、天冬氨酸蛋白酶签名序列、酶活化位点和非消化性组织蛋白酶D的特征序列,并且呈保守的双叶形结构。同源性比较和系统进化分析发现,MjCatD与斑节对虾、美洲螯龙虾和脊尾白虾相似性较高,并且与它们紧密聚为一支。实时荧光定量PCR结果显示,MjCatD基因在日本囊对虾多个组织中均有表达,其中肝胰腺中表达量最高。在白斑综合征病毒(white spot syndrome virus, WSSV)感染后3～24 h日本囊对虾肝胰腺中MjCatD的表达量逐渐下降,而在48 h急剧上调至最高表达量并且与对照组差异极显著(P<0.01)。此外,MjCatD基因在幼体发育不同阶段中也表现出明显的变化趋势。以上研究表明,MjCatD基因可能参与日本囊对虾先天免疫反应和幼体发育过程。     

Discussion on applying an analytical method to optimize the anti-freeze design parameters for underground water pipelines in seasonally frozen areas

Adopting the quasi-three-dimensional (Quasi-3D) numerical method to optimize the anti-freeze design parameters of an underground pipeline usually involves heavy numerical calculations. Here, the fitting formulae between the safe conveyance distance (SCD) of a water pipeline and six influencing factors are established based on the lowest water temperature (LWT) along the pipeline axis direction. With reference to the current widely used anti-freeze design approaches for underground pipelines in seasonally frozen areas, this paper first analyzes the feasibility of applying the maximum frozen penetration (MFP) instead of the mean annual ground surface temperature (MAGST) and soil water content (SWC) to calculate the SCD. The results show that the SCD depends on the buried depth if the MFP is fixed and the variation of the MAGST and SWC combination does not significantly change the SCD. A comprehensive formula for the SCD is established based on the relationships between the SCD and several primary influencing factors and the interaction among them. This formula involves five easy-to-access parameters: the MFP, buried depth, pipeline diameter, flow velocity, and inlet water temperature. A comparison between the analytical method and the numerical results based on the Quasi-3D method indicates that the two methods are in good agreement overall. The analytic method can be used to optimize the anti-freeze design parameters of underground water pipelines in seasonally frozen areas under the condition of a 1.5 safety coefficient.     

Effects of initial step height on the headcut erosion of bank gullies: a case study using a 3D photo-reconstruction method in the dry-hot valley region of southwest China

Abstract

Headcut erosion has been recognized as one of the main processes involved in gully development in the dry-hot valley region of southwest China. To examine the effect of initial step height on headcut erosion processes, three headcuts were constructed ranging in height from 0.75 to 1.25 m on an active bank gully head, and a series of scouring experiments were conducted under a flow discharge of 120 L min^?1. The morphological evolutions of the plunge pools and soil loss volume were estimated by three-dimensional photo-reconstruction methods (3D-PR). As the step height increased, the experimental results showed that: (1) the transformed potential energy and shear stress would increase by approximately 4.89 J s^?1 and 26.4 Pa on average when the step height increased 0.25 m; (2) the mean depth and width of the plunge pool exhibited obvious growth, and the morphology of the cross-section developed from approximately V-shaped to U-shaped; and (3) soil loss volume increased logarithmically, with total soil loss volumes of 0.076, 0.105 and 0.116 m³, respectively. Although the significant effects of the initial step height on headcut erosion were verified, further quantitative studies are required to quantify the mechanism of headcut erosion, especially for plunge pool erosion.     

FLAC3D在边坡数值分析及位移监测中应用

以辽宁省岫岩县某山头边坡为例,通过槽探、钻探、大型剪切试验等多种手段,找出合理潜在滑动面。利用FLAC3D软件对该边坡进行三维数值模拟,分析应力应变关系,得出变形较大及应力集中区域,同时利用强度折减法得出边坡的安全系数,评价其稳定性。将从边坡后缘张拉裂隙上布设的位移监测点采集到的数据,与数值模拟结果进行对比分析,验证数值模拟的可靠性,为边坡抗滑设计提供依据。     

3D打印技术在矿床学中的应用研究——以内蒙古乌力吉敖包萤石矿Ⅲ号矿体为例

3D打印(3D printing)是一种以三维模型为样本的快速成形技术,它通过逐层打印或粉末熔铸的方式来构造需要的立体图形。本文从3D打印技术的原理及其发展现状入手,重点列举内蒙古乌力吉敖包萤石矿,利用3D打印机打印具有现实可观性的地质体实体,探讨3D打印技术作为新兴的科技手段应用于矿床学教学与研究的应用价值和发展前景。研究发现,3D打印技术在矿体三维可视化中的应用符合地质图件立体化和3D打印技术多领域化的趋势,未来3D打印技术在地质领域的应用也有助于开拓矿床学教学和研究的新方式与新思路。     

塔里木溢流玄武岩火山通道的三维结构及其热成因气体释放

塔里木上奥陶统-志留系沉积地层中广泛发育早二叠世溢流玄武岩的火山通道相岩床-岩墙网络。三维地震数据解释结果显示,这些火山通道以平行围岩地层的岩床和斜切围岩地层的"碟状岩床"为主要特征。在玄武岩喷发过程中,火山通道岩浆的热量可以导致沉积围岩发生热接触变质并将沉积围岩中的有机质转化为"热成因气体"。在塔北英买2井区火山通道烘烤沉积围岩模型基础上,利用有限元热模拟方法确定了该区早二叠世玄武岩喷发时火山通道热烘烤影响范围随时间的变化。基于沉积围岩有机质丰度估算,该区热烘烤成因甲烷释放量可达11.3Gt(即113亿吨)。如果整个塔里木溢流玄武岩省具有与英买2地区相同的释放强度,则塔里木溢流玄武岩省活动期间释放的甲烷总量可达7062.5Gt,必然导致非常显著的环境效应。同时,玄武岩火山通道岩浆引起的热接触变质作用对已存在的油藏具有明显的破坏作用,塔里木盆地古生界总量约8~10Gt的油藏破坏和大量沥青的形成可能与此有关。     

地震叠前反演方法预测页岩脆性指数在南川地区的应用

页岩气的有效开发依赖水平井的压裂效果,而页岩脆性是影响页岩压裂的关键因素。通过对比分析现有的3种脆性指数计算方法,即矿物脆性指数计算法、岩石力学脆性指数计算法、基于多元回归脆性指数计算法,优选基于多元回归的脆性指数计算法计算五峰—龙马溪组(简称龙马溪组)页岩储层脆性指数。以四川盆地东部南川地区龙马溪组海相页岩气为例,利用南川地区三维地震资料叠前反演方法,获取多元回归脆性指数计算方程所需的参数,预测页岩脆性指数。结果表明,在已有钻井矿物脆性指标约束下,叠前反演法计算的脆性指数平面预测效果较好,且与实际压裂数据具有较好的一致性,能够满足南川地区页岩气勘探开发的需要。     

A virtual globe-based time-critical adaptive visualization method for 3D city models

3D city models, which are important items of content on the virtual globe, are characterized by complicated structures and large amounts of data. These factors make the visualization of 3D city models highly dependent upon the performance of computer hardware. Thus, achieving the efficient rendering of 3D city models using different hardware performance levels represents one of the key problems currently facing researchers. This paper proposes a time-critical adaptive visualization method that first estimates the possible rendering time for each model according to the data structure of the model in addition to the CPU/GPU performance of the computer. It then dynamically adjusts the rendering level for each model based on the results of an estimation of the rendering time to ensure that the final scene can be completed within a given time. To verify the effectiveness and flexibility of this method, it is applied using different computers. The results show that the adaptive visualization method presented in this paper not only can adapt to computers with different levels of performances but also demonstrates an obvious improvement in the time estimation precision, visual effects, and optimization speed relative to existing adaptive visualization methods.     

广东省仁化县书楼丘铀矿床三维地质建模及成矿预测

综合地质、地球化学等资料进行多元信息成矿预测是目前深部固体矿产主要工作方法。广东仁化县书楼丘铀矿床位于诸广南铀矿田北部,是诸广南铀矿田重要的组成之一。本次为了拓宽书楼丘矿床深部找矿思路,探讨其找矿潜力,在前人研究和勘探基础上,总结了矿床综合信息找矿模型,以三维定量预测研究理论基础,根据矿床成矿条件和找矿标志特征,提取地质、化探、钻探资料的成矿有利信息,并利用COCAD软件构建了地形-地表、岩体、构造、矿体等地质模型,对信息进行综合成矿有利分析,运用信息量法进行研究区深部矿产资源预测,共圈定了3片靶区。     

磁法三维建模在龙王沟磁铁矿勘探中的应用

以龙王沟磁铁矿高精度磁测数据为基础,采用小波多尺度分解方法,对化极后的磁法数据进行多尺度分解,通过功率谱法计算小波多尺度分解后的各阶细节异常的场源深度。结合地形、地质及钻孔资料,采用2.5D人机交互反演对磁测剖面上的磁铁矿体进行拟合,利用Golden Software公司开发的Voxler软件,在虚拟三维环境下实现二维小波多尺度分解数据的可视化,建立三维地质体模型,直观的预测磁铁矿体的空间范围和分布形态,实现地质、物探和地上、地下信息一体化集成展示,进行专业分析评价和辅助决策,可以有效的提高地质找矿效果。